4WJY
Esherichia coli nitrite reductase NrfA H264N
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0005506 | molecular_function | iron ion binding |
| A | 0005509 | molecular_function | calcium ion binding |
| A | 0009061 | biological_process | anaerobic respiration |
| A | 0016491 | molecular_function | oxidoreductase activity |
| A | 0016966 | molecular_function | nitric oxide reductase activity |
| A | 0019645 | biological_process | anaerobic electron transport chain |
| A | 0020037 | molecular_function | heme binding |
| A | 0030288 | cellular_component | outer membrane-bounded periplasmic space |
| A | 0042128 | biological_process | nitrate assimilation |
| A | 0042279 | molecular_function | nitrite reductase (cytochrome, ammonia-forming) activity |
| A | 0042597 | cellular_component | periplasmic space |
| A | 0046872 | molecular_function | metal ion binding |
| B | 0005506 | molecular_function | iron ion binding |
| B | 0005509 | molecular_function | calcium ion binding |
| B | 0009061 | biological_process | anaerobic respiration |
| B | 0016491 | molecular_function | oxidoreductase activity |
| B | 0016966 | molecular_function | nitric oxide reductase activity |
| B | 0019645 | biological_process | anaerobic electron transport chain |
| B | 0020037 | molecular_function | heme binding |
| B | 0030288 | cellular_component | outer membrane-bounded periplasmic space |
| B | 0042128 | biological_process | nitrate assimilation |
| B | 0042279 | molecular_function | nitrite reductase (cytochrome, ammonia-forming) activity |
| B | 0042597 | cellular_component | periplasmic space |
| B | 0046872 | molecular_function | metal ion binding |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 23 |
| Details | binding site for residue HEC A 501 |
| Chain | Residue |
| A | ASN89 |
| A | TYR216 |
| A | VAL225 |
| A | ASN264 |
| A | HIS388 |
| A | HEC503 |
| A | HOH623 |
| A | HOH638 |
| A | HOH640 |
| A | HOH672 |
| A | HOH693 |
| A | ASP100 |
| A | HOH719 |
| A | HOH762 |
| A | HOH805 |
| A | HOH898 |
| A | THR104 |
| A | ARG106 |
| A | CYS122 |
| A | CYS125 |
| A | LYS126 |
| A | CYS212 |
| A | HIS213 |
| site_id | AC2 |
| Number of Residues | 22 |
| Details | binding site for residue HEC A 502 |
| Chain | Residue |
| A | HIS49 |
| A | GLN52 |
| A | TYR53 |
| A | TRP56 |
| A | GLY159 |
| A | CYS160 |
| A | CYS163 |
| A | HIS164 |
| A | PHE171 |
| A | GLY174 |
| A | PRO176 |
| A | ARG201 |
| A | GLN205 |
| A | ILE283 |
| A | MET287 |
| A | LYS289 |
| A | TYR298 |
| A | THR299 |
| A | HIS301 |
| A | HEC503 |
| A | HOH688 |
| A | HOH696 |
| site_id | AC3 |
| Number of Residues | 24 |
| Details | binding site for residue HEC A 503 |
| Chain | Residue |
| A | GLN62 |
| A | PRO91 |
| A | ARG92 |
| A | GLY93 |
| A | HIS94 |
| A | PHE96 |
| A | ALA97 |
| A | ASP100 |
| A | CYS125 |
| A | LEU158 |
| A | VAL208 |
| A | CYS209 |
| A | CYS212 |
| A | HIS213 |
| A | HIS286 |
| A | MET287 |
| A | ILE303 |
| A | HEC501 |
| A | HEC502 |
| A | HEC504 |
| A | CA507 |
| A | HOH708 |
| A | HOH791 |
| A | HOH799 |
| site_id | AC4 |
| Number of Residues | 23 |
| Details | binding site for residue HEC A 504 |
| Chain | Residue |
| A | HIS213 |
| A | TRP270 |
| A | HIS275 |
| A | VAL280 |
| A | THR281 |
| A | CYS282 |
| A | CYS285 |
| A | HIS286 |
| A | ASN305 |
| A | PRO306 |
| A | VAL329 |
| A | HIS388 |
| A | HIS391 |
| A | MET392 |
| A | HIS393 |
| A | HEC503 |
| A | HEC505 |
| A | CA507 |
| A | HOH742 |
| A | HOH779 |
| A | HOH786 |
| A | HOH845 |
| A | HOH859 |
| site_id | AC5 |
| Number of Residues | 16 |
| Details | binding site for residue HEC A 505 |
| Chain | Residue |
| A | ARG332 |
| A | MET392 |
| A | HEC504 |
| A | EDO508 |
| A | HOH826 |
| A | HOH862 |
| B | HIS318 |
| B | GLN320 |
| B | HEC505 |
| A | HIS275 |
| A | VAL280 |
| A | PHE307 |
| A | THR313 |
| A | CYS314 |
| A | CYS317 |
| A | HIS318 |
| site_id | AC6 |
| Number of Residues | 6 |
| Details | binding site for residue CA A 506 |
| Chain | Residue |
| A | GLU215 |
| A | TYR216 |
| A | LYS261 |
| A | GLN263 |
| A | HOH657 |
| A | HOH765 |
| site_id | AC7 |
| Number of Residues | 7 |
| Details | binding site for residue CA A 507 |
| Chain | Residue |
| A | PRO91 |
| A | HEC503 |
| A | HEC504 |
| A | HOH742 |
| A | HOH779 |
| A | HOH799 |
| A | HOH906 |
| site_id | AC8 |
| Number of Residues | 3 |
| Details | binding site for residue EDO A 508 |
| Chain | Residue |
| A | CYS317 |
| A | HEC505 |
| B | HEC505 |
| site_id | AC9 |
| Number of Residues | 4 |
| Details | binding site for residue EDO A 509 |
| Chain | Residue |
| A | ASP308 |
| A | PHE310 |
| A | ALA311 |
| A | LYS322 |
| site_id | AD1 |
| Number of Residues | 22 |
| Details | binding site for residue HEC B 501 |
| Chain | Residue |
| B | TYR88 |
| B | ASN89 |
| B | ASP100 |
| B | THR104 |
| B | ARG106 |
| B | LEU118 |
| B | CYS122 |
| B | CYS125 |
| B | LYS126 |
| B | CYS212 |
| B | HIS213 |
| B | TYR216 |
| B | ASN264 |
| B | HIS388 |
| B | HEC503 |
| B | HOH639 |
| B | HOH658 |
| B | HOH663 |
| B | HOH679 |
| B | HOH715 |
| B | HOH721 |
| B | HOH788 |
| site_id | AD2 |
| Number of Residues | 22 |
| Details | binding site for residue HEC B 502 |
| Chain | Residue |
| B | HIS49 |
| B | GLN52 |
| B | TYR53 |
| B | TRP56 |
| B | GLY159 |
| B | CYS160 |
| B | CYS163 |
| B | HIS164 |
| B | PHE171 |
| B | GLY174 |
| B | LEU178 |
| B | ARG201 |
| B | GLN205 |
| B | MET287 |
| B | LYS289 |
| B | TYR298 |
| B | THR299 |
| B | HIS301 |
| B | HEC503 |
| B | HOH702 |
| B | HOH707 |
| B | HOH834 |
| site_id | AD3 |
| Number of Residues | 6 |
| Details | binding site for residue CA B 506 |
| Chain | Residue |
| B | GLU215 |
| B | TYR216 |
| B | LYS261 |
| B | GLN263 |
| B | HOH624 |
| B | HOH692 |
| site_id | AD4 |
| Number of Residues | 6 |
| Details | binding site for residue CA B 507 |
| Chain | Residue |
| B | PRO91 |
| B | HEC503 |
| B | HEC504 |
| B | HOH778 |
| B | HOH809 |
| B | HOH856 |
| site_id | AD5 |
| Number of Residues | 7 |
| Details | binding site for residue EDO B 508 |
| Chain | Residue |
| B | ARG74 |
| B | ASP345 |
| B | HIS349 |
| B | ILE431 |
| B | ILE434 |
| B | HOH698 |
| B | HOH741 |
| site_id | AD6 |
| Number of Residues | 28 |
| Details | binding site for Di-peptide HEC B 503 and CYS B 209 |
| Chain | Residue |
| B | GLN62 |
| B | PRO91 |
| B | ARG92 |
| B | GLY93 |
| B | HIS94 |
| B | PHE96 |
| B | ALA97 |
| B | ASP100 |
| B | CYS125 |
| B | LYS126 |
| B | LEU158 |
| B | SER206 |
| B | MET207 |
| B | VAL208 |
| B | GLY210 |
| B | GLN211 |
| B | CYS212 |
| B | HIS213 |
| B | CYS282 |
| B | HIS286 |
| B | ILE303 |
| B | HEC501 |
| B | HEC502 |
| B | HEC504 |
| B | CA507 |
| B | HOH762 |
| B | HOH778 |
| B | HOH825 |
| site_id | AD7 |
| Number of Residues | 19 |
| Details | binding site for Di-peptide HEC B 504 and CYS B 282 |
| Chain | Residue |
| B | SER206 |
| B | CYS209 |
| B | HIS213 |
| B | TRP270 |
| B | HIS275 |
| B | THR281 |
| B | ILE283 |
| B | ASP284 |
| B | CYS285 |
| B | HIS286 |
| B | HIS388 |
| B | HIS391 |
| B | MET392 |
| B | HIS393 |
| B | HEC503 |
| B | HEC505 |
| B | CA507 |
| B | HOH687 |
| B | HOH809 |
| site_id | AD8 |
| Number of Residues | 20 |
| Details | binding site for Di-peptide HEC B 505 and CYS B 314 |
| Chain | Residue |
| A | HIS318 |
| A | GLN320 |
| A | HEC505 |
| A | EDO508 |
| B | ILE274 |
| B | HIS275 |
| B | ASN278 |
| B | VAL280 |
| B | PHE307 |
| B | PHE310 |
| B | THR313 |
| B | ALA315 |
| B | ASN316 |
| B | CYS317 |
| B | HIS318 |
| B | ARG332 |
| B | MET392 |
| B | HEC504 |
| B | HOH789 |
| B | HOH818 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 18 |
| Details | BINDING: axial binding residue => ECO:0007744|PDB:1GU6, ECO:0007744|PDB:2RDZ, ECO:0007744|PDB:2RF7, ECO:0007744|PDB:3L1T, ECO:0007744|PDB:3TOR |
| Chain | Residue | Details |
| A | HIS94 | |
| B | HIS94 | |
| B | LYS126 | |
| B | HIS164 | |
| B | HIS213 | |
| B | HIS275 | |
| B | HIS286 | |
| B | HIS301 | |
| B | HIS318 | |
| B | HIS393 | |
| A | LYS126 | |
| A | HIS164 | |
| A | HIS213 | |
| A | HIS275 | |
| A | HIS286 | |
| A | HIS301 | |
| A | HIS318 | |
| A | HIS393 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 16 |
| Details | BINDING: covalent => ECO:0007744|PDB:1GU6, ECO:0007744|PDB:2RDZ, ECO:0007744|PDB:2RF7, ECO:0007744|PDB:3L1T, ECO:0007744|PDB:3TOR |
| Chain | Residue | Details |
| A | CYS122 | |
| B | CYS125 | |
| B | CYS160 | |
| B | CYS163 | |
| B | CYS209 | |
| B | CYS212 | |
| B | CYS282 | |
| B | CYS314 | |
| A | CYS125 | |
| A | CYS160 | |
| A | CYS163 | |
| A | CYS209 | |
| A | CYS212 | |
| A | CYS282 | |
| A | CYS314 | |
| B | CYS122 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 4 |
| Details | BINDING: BINDING => ECO:0007744|PDB:1GU6, ECO:0007744|PDB:2RDZ, ECO:0007744|PDB:2RF7, ECO:0007744|PDB:3L1T, ECO:0007744|PDB:3TOR |
| Chain | Residue | Details |
| A | GLU215 | |
| A | LYS261 | |
| B | GLU215 | |
| B | LYS261 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 4 |
| Details | BINDING: BINDING => ECO:0000305 |
| Chain | Residue | Details |
| A | TYR216 | |
| A | ASN264 | |
| B | TYR216 | |
| B | ASN264 |
| site_id | SWS_FT_FI5 |
| Number of Residues | 2 |
| Details | BINDING: BINDING => ECO:0007744|PDB:1GU6, ECO:0007744|PDB:2RDZ, ECO:0007744|PDB:3L1T, ECO:0007744|PDB:3TOR |
| Chain | Residue | Details |
| A | GLN263 | |
| B | GLN263 |
| site_id | SWS_FT_FI6 |
| Number of Residues | 4 |
| Details | BINDING: covalent => ECO:0007744|PDB:1GU6, ECO:0007744|PDB:2RDZ, ECO:0007744|PDB:2RF7 |
| Chain | Residue | Details |
| A | CYS285 | |
| A | CYS317 | |
| B | CYS285 | |
| B | CYS317 |
