4WF2

Structure of E. coli BirA G142A bound to biotinol-5'-AMP

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000166	molecular_function	nucleotide binding
A	0000976	molecular_function	transcription cis-regulatory region binding
A	0003676	molecular_function	nucleic acid binding
A	0003677	molecular_function	DNA binding
A	0004077	molecular_function	biotin--[biotin carboxyl-carrier protein] ligase activity
A	0005515	molecular_function	protein binding
A	0005524	molecular_function	ATP binding
A	0005737	cellular_component	cytoplasm
A	0006355	biological_process	regulation of DNA-templated transcription
A	0006768	biological_process	biotin metabolic process
A	0009102	biological_process	biotin biosynthetic process
A	0009374	molecular_function	biotin binding
A	0016874	molecular_function	ligase activity
A	0017053	cellular_component	transcription repressor complex
A	0036211	biological_process	protein modification process
A	0042803	molecular_function	protein homodimerization activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	20
Details	binding site for residue BTX A 501

Chain	Residue
A	SER89
A	TRP123
A	PHE124
A	SER134
A	LYS183
A	GLY186
A	ILE187
A	ALA205
A	GLY206
A	ASN208
A	TRP223
A	THR90
A	HOH652
A	GLN112
A	GLY115
A	ARG116
A	GLY117
A	ARG118
A	ARG121
A	LYS122

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	19
Details	DNA_BIND: H-T-H motif => ECO:0000255|HAMAP-Rule:MF_00978

Chain	Residue	Details
A	GLY22-GLN41

---

site_id	SWS_FT_FI2
Number of Residues	2
Details	BINDING:

Chain	Residue	Details
A	SER89
A	ARG116

---

site_id	SWS_FT_FI3
Number of Residues	2
Details	BINDING: BINDING => ECO:0000255|HAMAP-Rule:MF_00978, ECO:0000269|PubMed:11353844, ECO:0000269|PubMed:1409631

Chain	Residue	Details
A	GLN112
A	LYS183

---

Catalytic Information from CSA

site_id	MCSA1
Number of Residues	3
Details	M-CSA 538

Chain	Residue	Details
A	ARG118	electrostatic stabiliser
A	LYS183	electrostatic stabiliser
A	ARG317

---