Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

4V45

E. COLI (lacZ) BETA-GALACTOSIDASE-TRAPPED 2-F-GALACTOSYL-ENZYME INTERMEDIATE

This is a non-PDB format compatible entry.

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000287	molecular_function	magnesium ion binding
A	0003824	molecular_function	catalytic activity
A	0004553	molecular_function	hydrolase activity, hydrolyzing O-glycosyl compounds
A	0004565	molecular_function	beta-galactosidase activity
A	0005975	biological_process	carbohydrate metabolic process
A	0005990	biological_process	lactose catabolic process
A	0009056	biological_process	catabolic process
A	0009341	cellular_component	beta-galactosidase complex
A	0016798	molecular_function	hydrolase activity, acting on glycosyl bonds
A	0030246	molecular_function	carbohydrate binding
A	0031420	molecular_function	alkali metal ion binding
A	0042802	molecular_function	identical protein binding
A	0046872	molecular_function	metal ion binding
B	0000287	molecular_function	magnesium ion binding
B	0003824	molecular_function	catalytic activity
B	0004553	molecular_function	hydrolase activity, hydrolyzing O-glycosyl compounds
B	0004565	molecular_function	beta-galactosidase activity
B	0005975	biological_process	carbohydrate metabolic process
B	0005990	biological_process	lactose catabolic process
B	0009056	biological_process	catabolic process
B	0009341	cellular_component	beta-galactosidase complex
B	0016798	molecular_function	hydrolase activity, acting on glycosyl bonds
B	0030246	molecular_function	carbohydrate binding
B	0031420	molecular_function	alkali metal ion binding
B	0042802	molecular_function	identical protein binding
B	0046872	molecular_function	metal ion binding
C	0000287	molecular_function	magnesium ion binding
C	0003824	molecular_function	catalytic activity
C	0004553	molecular_function	hydrolase activity, hydrolyzing O-glycosyl compounds
C	0004565	molecular_function	beta-galactosidase activity
C	0005975	biological_process	carbohydrate metabolic process
C	0005990	biological_process	lactose catabolic process
C	0009056	biological_process	catabolic process
C	0009341	cellular_component	beta-galactosidase complex
C	0016798	molecular_function	hydrolase activity, acting on glycosyl bonds
C	0030246	molecular_function	carbohydrate binding
C	0031420	molecular_function	alkali metal ion binding
C	0042802	molecular_function	identical protein binding
C	0046872	molecular_function	metal ion binding
D	0000287	molecular_function	magnesium ion binding
D	0003824	molecular_function	catalytic activity
D	0004553	molecular_function	hydrolase activity, hydrolyzing O-glycosyl compounds
D	0004565	molecular_function	beta-galactosidase activity
D	0005975	biological_process	carbohydrate metabolic process
D	0005990	biological_process	lactose catabolic process
D	0009056	biological_process	catabolic process
D	0009341	cellular_component	beta-galactosidase complex
D	0016798	molecular_function	hydrolase activity, acting on glycosyl bonds
D	0030246	molecular_function	carbohydrate binding
D	0031420	molecular_function	alkali metal ion binding
D	0042802	molecular_function	identical protein binding
D	0046872	molecular_function	metal ion binding
E	0000287	molecular_function	magnesium ion binding
E	0003824	molecular_function	catalytic activity
E	0004553	molecular_function	hydrolase activity, hydrolyzing O-glycosyl compounds
E	0004565	molecular_function	beta-galactosidase activity
E	0005975	biological_process	carbohydrate metabolic process
E	0005990	biological_process	lactose catabolic process
E	0009056	biological_process	catabolic process
E	0009341	cellular_component	beta-galactosidase complex
E	0016798	molecular_function	hydrolase activity, acting on glycosyl bonds
E	0030246	molecular_function	carbohydrate binding
E	0031420	molecular_function	alkali metal ion binding
E	0042802	molecular_function	identical protein binding
E	0046872	molecular_function	metal ion binding
F	0000287	molecular_function	magnesium ion binding
F	0003824	molecular_function	catalytic activity
F	0004553	molecular_function	hydrolase activity, hydrolyzing O-glycosyl compounds
F	0004565	molecular_function	beta-galactosidase activity
F	0005975	biological_process	carbohydrate metabolic process
F	0005990	biological_process	lactose catabolic process
F	0009056	biological_process	catabolic process
F	0009341	cellular_component	beta-galactosidase complex
F	0016798	molecular_function	hydrolase activity, acting on glycosyl bonds
F	0030246	molecular_function	carbohydrate binding
F	0031420	molecular_function	alkali metal ion binding
F	0042802	molecular_function	identical protein binding
F	0046872	molecular_function	metal ion binding
G	0000287	molecular_function	magnesium ion binding
G	0003824	molecular_function	catalytic activity
G	0004553	molecular_function	hydrolase activity, hydrolyzing O-glycosyl compounds
G	0004565	molecular_function	beta-galactosidase activity
G	0005975	biological_process	carbohydrate metabolic process
G	0005990	biological_process	lactose catabolic process
G	0009056	biological_process	catabolic process
G	0009341	cellular_component	beta-galactosidase complex
G	0016798	molecular_function	hydrolase activity, acting on glycosyl bonds
G	0030246	molecular_function	carbohydrate binding
G	0031420	molecular_function	alkali metal ion binding
G	0042802	molecular_function	identical protein binding
G	0046872	molecular_function	metal ion binding
H	0000287	molecular_function	magnesium ion binding
H	0003824	molecular_function	catalytic activity
H	0004553	molecular_function	hydrolase activity, hydrolyzing O-glycosyl compounds
H	0004565	molecular_function	beta-galactosidase activity
H	0005975	biological_process	carbohydrate metabolic process
H	0005990	biological_process	lactose catabolic process
H	0009056	biological_process	catabolic process
H	0009341	cellular_component	beta-galactosidase complex
H	0016798	molecular_function	hydrolase activity, acting on glycosyl bonds
H	0030246	molecular_function	carbohydrate binding
H	0031420	molecular_function	alkali metal ion binding
H	0042802	molecular_function	identical protein binding
H	0046872	molecular_function	metal ion binding
I	0000287	molecular_function	magnesium ion binding
I	0003824	molecular_function	catalytic activity
I	0004553	molecular_function	hydrolase activity, hydrolyzing O-glycosyl compounds
I	0004565	molecular_function	beta-galactosidase activity
I	0005975	biological_process	carbohydrate metabolic process
I	0005990	biological_process	lactose catabolic process
I	0009056	biological_process	catabolic process
I	0009341	cellular_component	beta-galactosidase complex
I	0016798	molecular_function	hydrolase activity, acting on glycosyl bonds
I	0030246	molecular_function	carbohydrate binding
I	0031420	molecular_function	alkali metal ion binding
I	0042802	molecular_function	identical protein binding
I	0046872	molecular_function	metal ion binding
J	0000287	molecular_function	magnesium ion binding
J	0003824	molecular_function	catalytic activity
J	0004553	molecular_function	hydrolase activity, hydrolyzing O-glycosyl compounds
J	0004565	molecular_function	beta-galactosidase activity
J	0005975	biological_process	carbohydrate metabolic process
J	0005990	biological_process	lactose catabolic process
J	0009056	biological_process	catabolic process
J	0009341	cellular_component	beta-galactosidase complex
J	0016798	molecular_function	hydrolase activity, acting on glycosyl bonds
J	0030246	molecular_function	carbohydrate binding
J	0031420	molecular_function	alkali metal ion binding
J	0042802	molecular_function	identical protein binding
J	0046872	molecular_function	metal ion binding
K	0000287	molecular_function	magnesium ion binding
K	0003824	molecular_function	catalytic activity
K	0004553	molecular_function	hydrolase activity, hydrolyzing O-glycosyl compounds
K	0004565	molecular_function	beta-galactosidase activity
K	0005975	biological_process	carbohydrate metabolic process
K	0005990	biological_process	lactose catabolic process
K	0009056	biological_process	catabolic process
K	0009341	cellular_component	beta-galactosidase complex
K	0016798	molecular_function	hydrolase activity, acting on glycosyl bonds
K	0030246	molecular_function	carbohydrate binding
K	0031420	molecular_function	alkali metal ion binding
K	0042802	molecular_function	identical protein binding
K	0046872	molecular_function	metal ion binding
L	0000287	molecular_function	magnesium ion binding
L	0003824	molecular_function	catalytic activity
L	0004553	molecular_function	hydrolase activity, hydrolyzing O-glycosyl compounds
L	0004565	molecular_function	beta-galactosidase activity
L	0005975	biological_process	carbohydrate metabolic process
L	0005990	biological_process	lactose catabolic process
L	0009056	biological_process	catabolic process
L	0009341	cellular_component	beta-galactosidase complex
L	0016798	molecular_function	hydrolase activity, acting on glycosyl bonds
L	0030246	molecular_function	carbohydrate binding
L	0031420	molecular_function	alkali metal ion binding
L	0042802	molecular_function	identical protein binding
L	0046872	molecular_function	metal ion binding
M	0000287	molecular_function	magnesium ion binding
M	0003824	molecular_function	catalytic activity
M	0004553	molecular_function	hydrolase activity, hydrolyzing O-glycosyl compounds
M	0004565	molecular_function	beta-galactosidase activity
M	0005975	biological_process	carbohydrate metabolic process
M	0005990	biological_process	lactose catabolic process
M	0009056	biological_process	catabolic process
M	0009341	cellular_component	beta-galactosidase complex
M	0016798	molecular_function	hydrolase activity, acting on glycosyl bonds
M	0030246	molecular_function	carbohydrate binding
M	0031420	molecular_function	alkali metal ion binding
M	0042802	molecular_function	identical protein binding
M	0046872	molecular_function	metal ion binding
N	0000287	molecular_function	magnesium ion binding
N	0003824	molecular_function	catalytic activity
N	0004553	molecular_function	hydrolase activity, hydrolyzing O-glycosyl compounds
N	0004565	molecular_function	beta-galactosidase activity
N	0005975	biological_process	carbohydrate metabolic process
N	0005990	biological_process	lactose catabolic process
N	0009056	biological_process	catabolic process
N	0009341	cellular_component	beta-galactosidase complex
N	0016798	molecular_function	hydrolase activity, acting on glycosyl bonds
N	0030246	molecular_function	carbohydrate binding
N	0031420	molecular_function	alkali metal ion binding
N	0042802	molecular_function	identical protein binding
N	0046872	molecular_function	metal ion binding
O	0000287	molecular_function	magnesium ion binding
O	0003824	molecular_function	catalytic activity
O	0004553	molecular_function	hydrolase activity, hydrolyzing O-glycosyl compounds
O	0004565	molecular_function	beta-galactosidase activity
O	0005975	biological_process	carbohydrate metabolic process
O	0005990	biological_process	lactose catabolic process
O	0009056	biological_process	catabolic process
O	0009341	cellular_component	beta-galactosidase complex
O	0016798	molecular_function	hydrolase activity, acting on glycosyl bonds
O	0030246	molecular_function	carbohydrate binding
O	0031420	molecular_function	alkali metal ion binding
O	0042802	molecular_function	identical protein binding
O	0046872	molecular_function	metal ion binding
P	0000287	molecular_function	magnesium ion binding
P	0003824	molecular_function	catalytic activity
P	0004553	molecular_function	hydrolase activity, hydrolyzing O-glycosyl compounds
P	0004565	molecular_function	beta-galactosidase activity
P	0005975	biological_process	carbohydrate metabolic process
P	0005990	biological_process	lactose catabolic process
P	0009056	biological_process	catabolic process
P	0009341	cellular_component	beta-galactosidase complex
P	0016798	molecular_function	hydrolase activity, acting on glycosyl bonds
P	0030246	molecular_function	carbohydrate binding
P	0031420	molecular_function	alkali metal ion binding
P	0042802	molecular_function	identical protein binding
P	0046872	molecular_function	metal ion binding

Functional Information from PROSITE/UniProt

site_id	PS00608
Number of Residues	15
Details	GLYCOSYL_HYDROL_F2_2 Glycosyl hydrolases family 2 acid/base catalyst. DRNHPSVIIWSlg.NE

Chain	Residue	Details
A	ASP447-GLU461

site_id	PS00719
Number of Residues	26
Details	GLYCOSYL_HYDROL_F2_1 Glycosyl hydrolases family 2 signature 1. NaVRCSHYPnhplWYtlcDryGLYVV

Chain	Residue	Details
A	ASN385-VAL410

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	16
Details	ACT_SITE: Proton donor => ECO:0000269\|PubMed:6420154

Chain	Residue	Details
C	SER462
D	SER462
E	SER462
F	SER462
G	SER462
H	SER462
I	SER462
J	SER462
K	SER462
L	SER462
M	SER462
N	SER462
O	SER462
P	SER462
B	SER462
A	SER462

site_id	SWS_FT_FI2
Number of Residues	16
Details	ACT_SITE: Nucleophile => ECO:0000269\|PubMed:1350782

Chain	Residue	Details
C	TYR538
D	TYR538
E	TYR538
F	TYR538
G	TYR538
H	TYR538
I	TYR538
J	TYR538
K	TYR538
L	TYR538
M	TYR538
N	TYR538
O	TYR538
P	TYR538
B	TYR538
A	TYR538

site_id	SWS_FT_FI3
Number of Residues	112
Details	BINDING:

Chain	Residue	Details
O	MET202
O	SER462
O	TYR538
O	CYS602
O	GLY605
O	SER1000
P	VAL103
P	MET202
P	SER462
P	TYR538
P	CYS602
P	GLY605
P	SER1000
E	CYS602
E	GLY605
E	SER1000
F	VAL103
F	MET202
F	SER462
F	TYR538
F	CYS602
F	GLY605
F	SER1000
G	VAL103
G	MET202
G	SER462
G	TYR538
G	CYS602
G	GLY605
G	SER1000
H	VAL103
H	MET202
H	SER462
H	TYR538
H	CYS602
H	GLY605
H	SER1000
I	VAL103
I	MET202
I	SER462
I	TYR538
I	CYS602
I	GLY605
I	SER1000
J	VAL103
J	MET202
J	SER462
J	TYR538
J	CYS602
J	GLY605
J	SER1000
K	VAL103
K	MET202
K	SER462
K	TYR538
K	CYS602
K	GLY605
K	SER1000
L	VAL103
L	MET202
L	SER462
L	TYR538
L	CYS602
L	GLY605
L	SER1000
M	VAL103
M	MET202
M	SER462
M	TYR538
M	CYS602
M	GLY605
M	SER1000
N	VAL103
N	MET202
N	SER462
N	TYR538
N	CYS602
N	GLY605
N	SER1000
O	VAL103
B	SER462
B	TYR538
B	CYS602
B	GLY605
B	SER1000
C	VAL103
C	MET202
C	SER462
C	TYR538
C	CYS602
C	GLY605
C	SER1000
D	VAL103
D	MET202
D	SER462
D	TYR538
D	CYS602
D	GLY605
D	SER1000
E	VAL103
E	MET202
E	SER462
E	TYR538
A	CYS602
A	GLY605
A	SER1000
B	VAL103
B	MET202
A	TYR538
A	MET202
A	SER462
A	VAL103

site_id	SWS_FT_FI4
Number of Residues	48
Details	BINDING: BINDING => ECO:0000269\|PubMed:11045615

Chain	Residue	Details
C	THR417
C	GLY419
D	THR417
D	GLY419
E	THR417
E	GLY419
F	THR417
F	GLY419
G	THR417
G	GLY419
H	THR417
H	GLY419
I	THR417
I	GLY419
J	THR417
J	GLY419
K	THR417
K	GLY419
L	THR417
L	GLY419
M	THR417
M	GLY419
N	THR417
N	GLY419
O	THR417
O	GLY419
P	THR417
P	GLY419
D	ASP598
E	ASP598
F	ASP598
G	ASP598
H	ASP598
I	ASP598
J	ASP598
K	ASP598
L	ASP598
M	ASP598
N	ASP598
O	ASP598
P	ASP598
C	ASP598
B	THR417
B	GLY419
B	ASP598
A	ASP598
A	THR417
A	GLY419

site_id	SWS_FT_FI5
Number of Residues	32
Details	SITE: Transition state stabilizer

Chain	Residue	Details
F	TYR392
G	GLU358
I	GLU358
I	TYR392
J	GLU358
J	TYR392
K	GLU358
K	TYR392
L	GLU358
L	TYR392
M	GLU358
M	TYR392
N	GLU358
N	TYR392
O	GLU358
O	TYR392
P	GLU358
P	TYR392
E	TYR392
F	GLU358
G	TYR392
H	GLU358
H	TYR392
B	GLU358
B	TYR392
C	GLU358
C	TYR392
D	GLU358
D	TYR392
E	GLU358
A	TYR392
A	GLU358

site_id	SWS_FT_FI6
Number of Residues	16
Details	SITE: Important for ensuring that an appropriate proportion of lactose is converted to allolactose

Chain	Residue	Details
L	SER1000
J	SER1000
K	SER1000
M	SER1000
N	SER1000
O	SER1000
P	SER1000
D	SER1000
E	SER1000
F	SER1000
G	SER1000
H	SER1000
I	SER1000
A	SER1000
B	SER1000
C	SER1000

Catalytic Information from CSA

site_id	MCSA1
Number of Residues	10
Details	M-CSA 422

Chain	Residue	Details
A	MET202
A	GLU358
A	TYR392
A	THR417
A	GLY419
A	SER462
A	TYR538
A	ASP598
A	CYS602
A	GLY605

site_id	MCSA10
Number of Residues	10
Details	M-CSA 422

Chain	Residue	Details
J	MET202
J	GLU358
J	TYR392
J	THR417
J	GLY419
J	SER462
J	TYR538
J	ASP598
J	CYS602
J	GLY605

site_id	MCSA11
Number of Residues	10
Details	M-CSA 422

Chain	Residue	Details
K	MET202
K	GLU358
K	TYR392
K	THR417
K	GLY419
K	SER462
K	TYR538
K	ASP598
K	CYS602
K	GLY605

site_id	MCSA12
Number of Residues	10
Details	M-CSA 422

Chain	Residue	Details
L	MET202
L	GLU358
L	TYR392
L	THR417
L	GLY419
L	SER462
L	TYR538
L	ASP598
L	CYS602
L	GLY605

site_id	MCSA13
Number of Residues	10
Details	M-CSA 422

Chain	Residue	Details
M	MET202
M	GLU358
M	TYR392
M	THR417
M	GLY419
M	SER462
M	TYR538
M	ASP598
M	CYS602
M	GLY605

site_id	MCSA14
Number of Residues	10
Details	M-CSA 422

Chain	Residue	Details
N	MET202
N	GLU358
N	TYR392
N	THR417
N	GLY419
N	SER462
N	TYR538
N	ASP598
N	CYS602
N	GLY605

site_id	MCSA15
Number of Residues	10
Details	M-CSA 422

Chain	Residue	Details
O	MET202
O	GLU358
O	TYR392
O	THR417
O	GLY419
O	SER462
O	TYR538
O	ASP598
O	CYS602
O	GLY605

site_id	MCSA16
Number of Residues	10
Details	M-CSA 422

Chain	Residue	Details
P	MET202
P	GLU358
P	TYR392
P	THR417
P	GLY419
P	SER462
P	TYR538
P	ASP598
P	CYS602
P	GLY605

site_id	MCSA2
Number of Residues	10
Details	M-CSA 422

Chain	Residue	Details
B	MET202
B	GLU358
B	TYR392
B	THR417
B	GLY419
B	SER462
B	TYR538
B	ASP598
B	CYS602
B	GLY605

site_id	MCSA3
Number of Residues	10
Details	M-CSA 422

Chain	Residue	Details
C	MET202
C	GLU358
C	TYR392
C	THR417
C	GLY419
C	SER462
C	TYR538
C	ASP598
C	CYS602
C	GLY605

site_id	MCSA4
Number of Residues	10
Details	M-CSA 422

Chain	Residue	Details
D	MET202
D	GLU358
D	TYR392
D	THR417
D	GLY419
D	SER462
D	TYR538
D	ASP598
D	CYS602
D	GLY605

site_id	MCSA5
Number of Residues	10
Details	M-CSA 422

Chain	Residue	Details
E	MET202
E	GLU358
E	TYR392
E	THR417
E	GLY419
E	SER462
E	TYR538
E	ASP598
E	CYS602
E	GLY605

site_id	MCSA6
Number of Residues	10
Details	M-CSA 422

Chain	Residue	Details
F	MET202
F	GLU358
F	TYR392
F	THR417
F	GLY419
F	SER462
F	TYR538
F	ASP598
F	CYS602
F	GLY605

site_id	MCSA7
Number of Residues	10
Details	M-CSA 422

Chain	Residue	Details
G	MET202
G	GLU358
G	TYR392
G	THR417
G	GLY419
G	SER462
G	TYR538
G	ASP598
G	CYS602
G	GLY605

site_id	MCSA8
Number of Residues	10
Details	M-CSA 422

Chain	Residue	Details
H	MET202
H	GLU358
H	TYR392
H	THR417
H	GLY419
H	SER462
H	TYR538
H	ASP598
H	CYS602
H	GLY605

site_id	MCSA9
Number of Residues	10
Details	M-CSA 422

Chain	Residue	Details
I	MET202
I	GLU358
I	TYR392
I	THR417
I	GLY419
I	SER462
I	TYR538
I	ASP598
I	CYS602
I	GLY605

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)