4V40

BETA-GALACTOSIDASE

This is a non-PDB format compatible entry.

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000287	molecular_function	magnesium ion binding
A	0003824	molecular_function	catalytic activity
A	0004553	molecular_function	hydrolase activity, hydrolyzing O-glycosyl compounds
A	0004565	molecular_function	beta-galactosidase activity
A	0005975	biological_process	carbohydrate metabolic process
A	0005990	biological_process	lactose catabolic process
A	0009341	cellular_component	beta-galactosidase complex
A	0016787	molecular_function	hydrolase activity
A	0016798	molecular_function	hydrolase activity, acting on glycosyl bonds
A	0030246	molecular_function	carbohydrate binding
A	0031420	molecular_function	alkali metal ion binding
A	0042802	molecular_function	identical protein binding
A	0046872	molecular_function	metal ion binding
B	0000287	molecular_function	magnesium ion binding
B	0003824	molecular_function	catalytic activity
B	0004553	molecular_function	hydrolase activity, hydrolyzing O-glycosyl compounds
B	0004565	molecular_function	beta-galactosidase activity
B	0005975	biological_process	carbohydrate metabolic process
B	0005990	biological_process	lactose catabolic process
B	0009341	cellular_component	beta-galactosidase complex
B	0016787	molecular_function	hydrolase activity
B	0016798	molecular_function	hydrolase activity, acting on glycosyl bonds
B	0030246	molecular_function	carbohydrate binding
B	0031420	molecular_function	alkali metal ion binding
B	0042802	molecular_function	identical protein binding
B	0046872	molecular_function	metal ion binding
C	0000287	molecular_function	magnesium ion binding
C	0003824	molecular_function	catalytic activity
C	0004553	molecular_function	hydrolase activity, hydrolyzing O-glycosyl compounds
C	0004565	molecular_function	beta-galactosidase activity
C	0005975	biological_process	carbohydrate metabolic process
C	0005990	biological_process	lactose catabolic process
C	0009341	cellular_component	beta-galactosidase complex
C	0016787	molecular_function	hydrolase activity
C	0016798	molecular_function	hydrolase activity, acting on glycosyl bonds
C	0030246	molecular_function	carbohydrate binding
C	0031420	molecular_function	alkali metal ion binding
C	0042802	molecular_function	identical protein binding
C	0046872	molecular_function	metal ion binding
D	0000287	molecular_function	magnesium ion binding
D	0003824	molecular_function	catalytic activity
D	0004553	molecular_function	hydrolase activity, hydrolyzing O-glycosyl compounds
D	0004565	molecular_function	beta-galactosidase activity
D	0005975	biological_process	carbohydrate metabolic process
D	0005990	biological_process	lactose catabolic process
D	0009341	cellular_component	beta-galactosidase complex
D	0016787	molecular_function	hydrolase activity
D	0016798	molecular_function	hydrolase activity, acting on glycosyl bonds
D	0030246	molecular_function	carbohydrate binding
D	0031420	molecular_function	alkali metal ion binding
D	0042802	molecular_function	identical protein binding
D	0046872	molecular_function	metal ion binding
E	0000287	molecular_function	magnesium ion binding
E	0003824	molecular_function	catalytic activity
E	0004553	molecular_function	hydrolase activity, hydrolyzing O-glycosyl compounds
E	0004565	molecular_function	beta-galactosidase activity
E	0005975	biological_process	carbohydrate metabolic process
E	0005990	biological_process	lactose catabolic process
E	0009341	cellular_component	beta-galactosidase complex
E	0016787	molecular_function	hydrolase activity
E	0016798	molecular_function	hydrolase activity, acting on glycosyl bonds
E	0030246	molecular_function	carbohydrate binding
E	0031420	molecular_function	alkali metal ion binding
E	0042802	molecular_function	identical protein binding
E	0046872	molecular_function	metal ion binding
F	0000287	molecular_function	magnesium ion binding
F	0003824	molecular_function	catalytic activity
F	0004553	molecular_function	hydrolase activity, hydrolyzing O-glycosyl compounds
F	0004565	molecular_function	beta-galactosidase activity
F	0005975	biological_process	carbohydrate metabolic process
F	0005990	biological_process	lactose catabolic process
F	0009341	cellular_component	beta-galactosidase complex
F	0016787	molecular_function	hydrolase activity
F	0016798	molecular_function	hydrolase activity, acting on glycosyl bonds
F	0030246	molecular_function	carbohydrate binding
F	0031420	molecular_function	alkali metal ion binding
F	0042802	molecular_function	identical protein binding
F	0046872	molecular_function	metal ion binding
G	0000287	molecular_function	magnesium ion binding
G	0003824	molecular_function	catalytic activity
G	0004553	molecular_function	hydrolase activity, hydrolyzing O-glycosyl compounds
G	0004565	molecular_function	beta-galactosidase activity
G	0005975	biological_process	carbohydrate metabolic process
G	0005990	biological_process	lactose catabolic process
G	0009341	cellular_component	beta-galactosidase complex
G	0016787	molecular_function	hydrolase activity
G	0016798	molecular_function	hydrolase activity, acting on glycosyl bonds
G	0030246	molecular_function	carbohydrate binding
G	0031420	molecular_function	alkali metal ion binding
G	0042802	molecular_function	identical protein binding
G	0046872	molecular_function	metal ion binding
H	0000287	molecular_function	magnesium ion binding
H	0003824	molecular_function	catalytic activity
H	0004553	molecular_function	hydrolase activity, hydrolyzing O-glycosyl compounds
H	0004565	molecular_function	beta-galactosidase activity
H	0005975	biological_process	carbohydrate metabolic process
H	0005990	biological_process	lactose catabolic process
H	0009341	cellular_component	beta-galactosidase complex
H	0016787	molecular_function	hydrolase activity
H	0016798	molecular_function	hydrolase activity, acting on glycosyl bonds
H	0030246	molecular_function	carbohydrate binding
H	0031420	molecular_function	alkali metal ion binding
H	0042802	molecular_function	identical protein binding
H	0046872	molecular_function	metal ion binding
I	0000287	molecular_function	magnesium ion binding
I	0003824	molecular_function	catalytic activity
I	0004553	molecular_function	hydrolase activity, hydrolyzing O-glycosyl compounds
I	0004565	molecular_function	beta-galactosidase activity
I	0005975	biological_process	carbohydrate metabolic process
I	0005990	biological_process	lactose catabolic process
I	0009341	cellular_component	beta-galactosidase complex
I	0016787	molecular_function	hydrolase activity
I	0016798	molecular_function	hydrolase activity, acting on glycosyl bonds
I	0030246	molecular_function	carbohydrate binding
I	0031420	molecular_function	alkali metal ion binding
I	0042802	molecular_function	identical protein binding
I	0046872	molecular_function	metal ion binding
J	0000287	molecular_function	magnesium ion binding
J	0003824	molecular_function	catalytic activity
J	0004553	molecular_function	hydrolase activity, hydrolyzing O-glycosyl compounds
J	0004565	molecular_function	beta-galactosidase activity
J	0005975	biological_process	carbohydrate metabolic process
J	0005990	biological_process	lactose catabolic process
J	0009341	cellular_component	beta-galactosidase complex
J	0016787	molecular_function	hydrolase activity
J	0016798	molecular_function	hydrolase activity, acting on glycosyl bonds
J	0030246	molecular_function	carbohydrate binding
J	0031420	molecular_function	alkali metal ion binding
J	0042802	molecular_function	identical protein binding
J	0046872	molecular_function	metal ion binding
K	0000287	molecular_function	magnesium ion binding
K	0003824	molecular_function	catalytic activity
K	0004553	molecular_function	hydrolase activity, hydrolyzing O-glycosyl compounds
K	0004565	molecular_function	beta-galactosidase activity
K	0005975	biological_process	carbohydrate metabolic process
K	0005990	biological_process	lactose catabolic process
K	0009341	cellular_component	beta-galactosidase complex
K	0016787	molecular_function	hydrolase activity
K	0016798	molecular_function	hydrolase activity, acting on glycosyl bonds
K	0030246	molecular_function	carbohydrate binding
K	0031420	molecular_function	alkali metal ion binding
K	0042802	molecular_function	identical protein binding
K	0046872	molecular_function	metal ion binding
L	0000287	molecular_function	magnesium ion binding
L	0003824	molecular_function	catalytic activity
L	0004553	molecular_function	hydrolase activity, hydrolyzing O-glycosyl compounds
L	0004565	molecular_function	beta-galactosidase activity
L	0005975	biological_process	carbohydrate metabolic process
L	0005990	biological_process	lactose catabolic process
L	0009341	cellular_component	beta-galactosidase complex
L	0016787	molecular_function	hydrolase activity
L	0016798	molecular_function	hydrolase activity, acting on glycosyl bonds
L	0030246	molecular_function	carbohydrate binding
L	0031420	molecular_function	alkali metal ion binding
L	0042802	molecular_function	identical protein binding
L	0046872	molecular_function	metal ion binding
M	0000287	molecular_function	magnesium ion binding
M	0003824	molecular_function	catalytic activity
M	0004553	molecular_function	hydrolase activity, hydrolyzing O-glycosyl compounds
M	0004565	molecular_function	beta-galactosidase activity
M	0005975	biological_process	carbohydrate metabolic process
M	0005990	biological_process	lactose catabolic process
M	0009341	cellular_component	beta-galactosidase complex
M	0016787	molecular_function	hydrolase activity
M	0016798	molecular_function	hydrolase activity, acting on glycosyl bonds
M	0030246	molecular_function	carbohydrate binding
M	0031420	molecular_function	alkali metal ion binding
M	0042802	molecular_function	identical protein binding
M	0046872	molecular_function	metal ion binding
N	0000287	molecular_function	magnesium ion binding
N	0003824	molecular_function	catalytic activity
N	0004553	molecular_function	hydrolase activity, hydrolyzing O-glycosyl compounds
N	0004565	molecular_function	beta-galactosidase activity
N	0005975	biological_process	carbohydrate metabolic process
N	0005990	biological_process	lactose catabolic process
N	0009341	cellular_component	beta-galactosidase complex
N	0016787	molecular_function	hydrolase activity
N	0016798	molecular_function	hydrolase activity, acting on glycosyl bonds
N	0030246	molecular_function	carbohydrate binding
N	0031420	molecular_function	alkali metal ion binding
N	0042802	molecular_function	identical protein binding
N	0046872	molecular_function	metal ion binding
O	0000287	molecular_function	magnesium ion binding
O	0003824	molecular_function	catalytic activity
O	0004553	molecular_function	hydrolase activity, hydrolyzing O-glycosyl compounds
O	0004565	molecular_function	beta-galactosidase activity
O	0005975	biological_process	carbohydrate metabolic process
O	0005990	biological_process	lactose catabolic process
O	0009341	cellular_component	beta-galactosidase complex
O	0016787	molecular_function	hydrolase activity
O	0016798	molecular_function	hydrolase activity, acting on glycosyl bonds
O	0030246	molecular_function	carbohydrate binding
O	0031420	molecular_function	alkali metal ion binding
O	0042802	molecular_function	identical protein binding
O	0046872	molecular_function	metal ion binding
P	0000287	molecular_function	magnesium ion binding
P	0003824	molecular_function	catalytic activity
P	0004553	molecular_function	hydrolase activity, hydrolyzing O-glycosyl compounds
P	0004565	molecular_function	beta-galactosidase activity
P	0005975	biological_process	carbohydrate metabolic process
P	0005990	biological_process	lactose catabolic process
P	0009341	cellular_component	beta-galactosidase complex
P	0016787	molecular_function	hydrolase activity
P	0016798	molecular_function	hydrolase activity, acting on glycosyl bonds
P	0030246	molecular_function	carbohydrate binding
P	0031420	molecular_function	alkali metal ion binding
P	0042802	molecular_function	identical protein binding
P	0046872	molecular_function	metal ion binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	3
Details	binding site for residue MG A 1101

Chain	Residue
A	GLU416
A	HIS418
A	GLU461

---

site_id	AC2
Number of Residues	5
Details	binding site for residue MG A 1102

Chain	Residue
A	ASP15
A	ASN18
A	VAL21
A	GLN163
A	ASP193

---

site_id	AC3
Number of Residues	5
Details	binding site for residue MG B 1101

Chain	Residue
B	ASP201
B	GLU416
B	HIS418
B	GLU461
B	ASN102

---

site_id	AC4
Number of Residues	5
Details	binding site for residue MG B 1102

Chain	Residue
B	ASP15
B	ASN18
B	VAL21
B	GLN163
B	ASP193

---

site_id	AC5
Number of Residues	3
Details	binding site for residue MG C 1101

Chain	Residue
C	GLU416
C	HIS418
C	GLU461

---

site_id	AC6
Number of Residues	5
Details	binding site for residue MG C 1102

Chain	Residue
C	ASP15
C	ASN18
C	VAL21
C	GLN163
C	ASP193

---

site_id	AC7
Number of Residues	4
Details	binding site for residue MG D 1101

Chain	Residue
D	ASP201
D	GLU416
D	HIS418
D	GLU461

---

site_id	AC8
Number of Residues	5
Details	binding site for residue MG D 1102

Chain	Residue
D	ASP15
D	ASN18
D	VAL21
D	GLN163
D	ASP193

---

site_id	AC9
Number of Residues	3
Details	binding site for residue MG E 1101

Chain	Residue
E	GLU416
E	HIS418
E	GLU461

---

site_id	AD1
Number of Residues	5
Details	binding site for residue MG E 1102

Chain	Residue
E	ASP15
E	ASN18
E	VAL21
E	GLN163
E	ASP193

---

site_id	AD2
Number of Residues	4
Details	binding site for residue MG F 1101

Chain	Residue
F	ASN102
F	GLU416
F	HIS418
F	GLU461

---

site_id	AD3
Number of Residues	6
Details	binding site for residue MG F 1102

Chain	Residue
F	ASP15
F	ASN18
F	VAL21
F	TYR161
F	GLN163
F	ASP193

---

site_id	AD4
Number of Residues	3
Details	binding site for residue MG G 1101

Chain	Residue
G	GLU416
G	HIS418
G	GLU461

---

site_id	AD5
Number of Residues	5
Details	binding site for residue MG G 1102

Chain	Residue
G	ASP15
G	ASN18
G	VAL21
G	GLN163
G	ASP193

---

site_id	AD6
Number of Residues	3
Details	binding site for residue MG H 1101

Chain	Residue
H	GLU416
H	HIS418
H	GLU461

---

site_id	AD7
Number of Residues	5
Details	binding site for residue MG H 1102

Chain	Residue
H	ASP15
H	ASN18
H	VAL21
H	GLN163
H	ASP193

---

site_id	AD8
Number of Residues	4
Details	binding site for residue MG I 1101

Chain	Residue
I	ASN102
I	GLU416
I	HIS418
I	GLU461

---

site_id	AD9
Number of Residues	5
Details	binding site for residue MG I 1102

Chain	Residue
I	ASP15
I	ASN18
I	VAL21
I	GLN163
I	ASP193

---

site_id	AE1
Number of Residues	4
Details	binding site for residue MG J 1101

Chain	Residue
J	ASN102
J	GLU416
J	HIS418
J	GLU461

---

site_id	AE2
Number of Residues	6
Details	binding site for residue MG J 1102

Chain	Residue
J	ASP15
J	ASN18
J	VAL21
J	TYR161
J	GLN163
J	ASP193

---

site_id	AE3
Number of Residues	5
Details	binding site for residue MG K 1101

Chain	Residue
K	ASN102
K	ASP201
K	GLU416
K	HIS418
K	GLU461

---

site_id	AE4
Number of Residues	5
Details	binding site for residue MG K 1102

Chain	Residue
K	ASP15
K	ASN18
K	VAL21
K	GLN163
K	ASP193

---

site_id	AE5
Number of Residues	3
Details	binding site for residue MG L 1101

Chain	Residue
L	HIS418
L	GLU461
L	GLU416

---

site_id	AE6
Number of Residues	5
Details	binding site for residue MG L 1102

Chain	Residue
L	ASP15
L	ASN18
L	VAL21
L	GLN163
L	ASP193

---

site_id	AE7
Number of Residues	3
Details	binding site for residue MG M 1101

Chain	Residue
M	GLU416
M	HIS418
M	GLU461

---

site_id	AE8
Number of Residues	5
Details	binding site for residue MG N 1101

Chain	Residue
N	ASN102
N	ASP201
N	GLU416
N	HIS418
N	GLU461

---

site_id	AE9
Number of Residues	5
Details	binding site for residue MG N 1102

Chain	Residue
N	ASP15
N	ASN18
N	VAL21
N	GLN163
N	ASP193

---

site_id	AF1
Number of Residues	5
Details	binding site for residue MG O 1101

Chain	Residue
O	ASN102
O	ASP201
O	GLU416
O	HIS418
O	GLU461

---

site_id	AF2
Number of Residues	5
Details	binding site for residue MG O 1102

Chain	Residue
O	ASP15
O	ASN18
O	VAL21
O	GLN163
O	ASP193

---

site_id	AF3
Number of Residues	3
Details	binding site for residue MG P 1101

Chain	Residue
P	GLU416
P	HIS418
P	GLU461

---

site_id	AF4
Number of Residues	5
Details	binding site for residue MG P 1102

Chain	Residue
P	ASP15
P	ASN18
P	VAL21
P	GLN163
P	ASP193

---

Functional Information from PROSITE/UniProt

site_id	PS00608
Number of Residues	15
Details	GLYCOSYL_HYDROL_F2_2 Glycosyl hydrolases family 2 acid/base catalyst. DRNHPSVIIWSlg.NE

Chain	Residue	Details
A	ASP447-GLU461

---

site_id	PS00719
Number of Residues	26
Details	GLYCOSYL_HYDROL_F2_1 Glycosyl hydrolases family 2 signature 1. NaVRCSHYPnhplWYtlcDryGLYVV

Chain	Residue	Details
A	ASN385-VAL410

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	16
Details	Active site: {"description":"Proton donor","evidences":[{"source":"PubMed","id":"6420154","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	16
Details	Active site: {"description":"Nucleophile","evidences":[{"source":"PubMed","id":"1350782","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	144
Details	Binding site: {}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	48
Details	Binding site: {"evidences":[{"source":"PubMed","id":"11045615","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	32
Details	Site: {"description":"Transition state stabilizer"}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	16
Details	Site: {"description":"Important for ensuring that an appropriate proportion of lactose is converted to allolactose"}

Chain

Residue

Details

---

Catalytic Information from CSA

site_id	MCSA1
Number of Residues	10
Details	M-CSA 422

Chain	Residue	Details
A	ASP201
A	ASN604
A	HIS357
A	HIS391
A	GLU416
A	HIS418
A	GLU461
A	GLU537
A	ASN597
A	PHE601

---

site_id	MCSA10
Number of Residues	10
Details	M-CSA 422

Chain	Residue	Details
J	ASP201
J	ASN604
J	HIS357
J	HIS391
J	GLU416
J	HIS418
J	GLU461
J	GLU537
J	ASN597
J	PHE601

---

site_id	MCSA11
Number of Residues	10
Details	M-CSA 422

Chain	Residue	Details
K	ASP201
K	ASN604
K	HIS357
K	HIS391
K	GLU416
K	HIS418
K	GLU461
K	GLU537
K	ASN597
K	PHE601

---

site_id	MCSA12
Number of Residues	10
Details	M-CSA 422

Chain	Residue	Details
L	ASP201
L	ASN604
L	HIS357
L	HIS391
L	GLU416
L	HIS418
L	GLU461
L	GLU537
L	ASN597
L	PHE601

---

site_id	MCSA13
Number of Residues	10
Details	M-CSA 422

Chain	Residue	Details
M	ASP201
M	ASN604
M	HIS357
M	HIS391
M	GLU416
M	HIS418
M	GLU461
M	GLU537
M	ASN597
M	PHE601

---

site_id	MCSA14
Number of Residues	10
Details	M-CSA 422

Chain	Residue	Details
N	ASP201
N	ASN604
N	HIS357
N	HIS391
N	GLU416
N	HIS418
N	GLU461
N	GLU537
N	ASN597
N	PHE601

---

site_id	MCSA15
Number of Residues	10
Details	M-CSA 422

Chain	Residue	Details
O	ASP201
O	ASN604
O	HIS357
O	HIS391
O	GLU416
O	HIS418
O	GLU461
O	GLU537
O	ASN597
O	PHE601

---

site_id	MCSA16
Number of Residues	10
Details	M-CSA 422

Chain	Residue	Details
P	ASP201
P	ASN604
P	HIS357
P	HIS391
P	GLU416
P	HIS418
P	GLU461
P	GLU537
P	ASN597
P	PHE601

---

site_id	MCSA2
Number of Residues	10
Details	M-CSA 422

Chain	Residue	Details
B	ASP201
B	ASN604
B	HIS357
B	HIS391
B	GLU416
B	HIS418
B	GLU461
B	GLU537
B	ASN597
B	PHE601

---

site_id	MCSA3
Number of Residues	10
Details	M-CSA 422

Chain	Residue	Details
C	ASP201
C	ASN604
C	HIS357
C	HIS391
C	GLU416
C	HIS418
C	GLU461
C	GLU537
C	ASN597
C	PHE601

---

site_id	MCSA4
Number of Residues	10
Details	M-CSA 422

Chain	Residue	Details
D	ASP201
D	ASN604
D	HIS357
D	HIS391
D	GLU416
D	HIS418
D	GLU461
D	GLU537
D	ASN597
D	PHE601

---

site_id	MCSA5
Number of Residues	10
Details	M-CSA 422

Chain	Residue	Details
E	ASP201
E	ASN604
E	HIS357
E	HIS391
E	GLU416
E	HIS418
E	GLU461
E	GLU537
E	ASN597
E	PHE601

---

site_id	MCSA6
Number of Residues	10
Details	M-CSA 422

Chain	Residue	Details
F	ASP201
F	ASN604
F	HIS357
F	HIS391
F	GLU416
F	HIS418
F	GLU461
F	GLU537
F	ASN597
F	PHE601

---

site_id	MCSA7
Number of Residues	10
Details	M-CSA 422

Chain	Residue	Details
G	ASP201
G	ASN604
G	HIS357
G	HIS391
G	GLU416
G	HIS418
G	GLU461
G	GLU537
G	ASN597
G	PHE601

---

site_id	MCSA8
Number of Residues	10
Details	M-CSA 422

Chain	Residue	Details
H	ASP201
H	ASN604
H	HIS357
H	HIS391
H	GLU416
H	HIS418
H	GLU461
H	GLU537
H	ASN597
H	PHE601

---

site_id	MCSA9
Number of Residues	10
Details	M-CSA 422

Chain	Residue	Details
I	ASP201
I	ASN604
I	HIS357
I	HIS391
I	GLU416
I	HIS418
I	GLU461
I	GLU537
I	ASN597
I	PHE601

---