4V0J
The channel-block Ser202Glu, Thr104Lys double mutant of Stearoyl-ACP- Desaturase from Castor bean (Ricinus communis)
Functional Information from GO Data
Chain | GOid | namespace | contents |
A | 0006631 | biological_process | fatty acid metabolic process |
A | 0006633 | biological_process | fatty acid biosynthetic process |
A | 0016491 | molecular_function | oxidoreductase activity |
A | 0045300 | molecular_function | acyl-[acyl-carrier-protein] desaturase activity |
B | 0006631 | biological_process | fatty acid metabolic process |
B | 0006633 | biological_process | fatty acid biosynthetic process |
B | 0016491 | molecular_function | oxidoreductase activity |
B | 0045300 | molecular_function | acyl-[acyl-carrier-protein] desaturase activity |
C | 0006631 | biological_process | fatty acid metabolic process |
C | 0006633 | biological_process | fatty acid biosynthetic process |
C | 0016491 | molecular_function | oxidoreductase activity |
C | 0045300 | molecular_function | acyl-[acyl-carrier-protein] desaturase activity |
D | 0006631 | biological_process | fatty acid metabolic process |
D | 0006633 | biological_process | fatty acid biosynthetic process |
D | 0016491 | molecular_function | oxidoreductase activity |
D | 0045300 | molecular_function | acyl-[acyl-carrier-protein] desaturase activity |
E | 0006631 | biological_process | fatty acid metabolic process |
E | 0006633 | biological_process | fatty acid biosynthetic process |
E | 0016491 | molecular_function | oxidoreductase activity |
E | 0045300 | molecular_function | acyl-[acyl-carrier-protein] desaturase activity |
F | 0006631 | biological_process | fatty acid metabolic process |
F | 0006633 | biological_process | fatty acid biosynthetic process |
F | 0016491 | molecular_function | oxidoreductase activity |
F | 0045300 | molecular_function | acyl-[acyl-carrier-protein] desaturase activity |
Functional Information from PDB Data
site_id | AC1 |
Number of Residues | 5 |
Details | BINDING SITE FOR RESIDUE FE2 A 364 |
Chain | Residue |
A | GLU105 |
A | GLU143 |
A | HIS146 |
A | GLU229 |
A | FE2365 |
site_id | AC2 |
Number of Residues | 5 |
Details | BINDING SITE FOR RESIDUE FE2 A 365 |
Chain | Residue |
A | FE2364 |
A | GLU143 |
A | GLU196 |
A | GLU229 |
A | HIS232 |
site_id | AC3 |
Number of Residues | 4 |
Details | BINDING SITE FOR RESIDUE FE2 B 364 |
Chain | Residue |
B | GLU105 |
B | GLU143 |
B | HIS146 |
B | GLU229 |
site_id | AC4 |
Number of Residues | 4 |
Details | BINDING SITE FOR RESIDUE FE2 B 365 |
Chain | Residue |
B | GLU143 |
B | GLU196 |
B | GLU229 |
B | HIS232 |
site_id | AC5 |
Number of Residues | 5 |
Details | BINDING SITE FOR RESIDUE FE2 C 364 |
Chain | Residue |
C | GLU105 |
C | GLU143 |
C | HIS146 |
C | GLU229 |
C | FE2365 |
site_id | AC6 |
Number of Residues | 5 |
Details | BINDING SITE FOR RESIDUE FE2 C 365 |
Chain | Residue |
C | GLU143 |
C | GLU196 |
C | GLU229 |
C | HIS232 |
C | FE2364 |
site_id | AC7 |
Number of Residues | 4 |
Details | BINDING SITE FOR RESIDUE FE2 D 364 |
Chain | Residue |
D | GLU105 |
D | GLU143 |
D | HIS146 |
D | GLU229 |
site_id | AC8 |
Number of Residues | 5 |
Details | BINDING SITE FOR RESIDUE FE2 D 365 |
Chain | Residue |
D | GLU143 |
D | GLU196 |
D | GLU229 |
D | HIS232 |
D | HOH2006 |
site_id | AC9 |
Number of Residues | 5 |
Details | BINDING SITE FOR RESIDUE FE2 E 364 |
Chain | Residue |
E | GLU105 |
E | GLU143 |
E | HIS146 |
E | GLU229 |
E | HOH2002 |
site_id | BC1 |
Number of Residues | 5 |
Details | BINDING SITE FOR RESIDUE FE2 E 365 |
Chain | Residue |
E | GLU143 |
E | GLU196 |
E | GLU229 |
E | HIS232 |
E | HOH2002 |
site_id | BC2 |
Number of Residues | 4 |
Details | BINDING SITE FOR RESIDUE FE2 F 364 |
Chain | Residue |
F | GLU105 |
F | GLU143 |
F | HIS146 |
F | GLU229 |
site_id | BC3 |
Number of Residues | 4 |
Details | BINDING SITE FOR RESIDUE FE2 F 365 |
Chain | Residue |
F | GLU143 |
F | GLU196 |
F | GLU229 |
F | HIS232 |
site_id | BC4 |
Number of Residues | 6 |
Details | BINDING SITE FOR RESIDUE GOL C 1364 |
Chain | Residue |
C | GLU106 |
C | ASN144 |
C | ASP148 |
D | GLU106 |
D | ASN144 |
D | ASP148 |
site_id | BC5 |
Number of Residues | 6 |
Details | BINDING SITE FOR RESIDUE GOL F 1364 |
Chain | Residue |
E | GLU106 |
E | ASN144 |
E | ASP148 |
F | GLU106 |
F | ASN144 |
F | ASP148 |
site_id | BC6 |
Number of Residues | 6 |
Details | BINDING SITE FOR RESIDUE GOL A 1364 |
Chain | Residue |
A | GLU106 |
A | ASN144 |
A | ASP148 |
B | GLU106 |
B | ASN144 |
B | ASP148 |
Functional Information from PROSITE/UniProt
site_id | PS00574 |
Number of Residues | 20 |
Details | FATTY_ACID_DESATUR_2 Fatty acid desaturases family 2 signature. SAvaqRLgvytakDYadILE |
Chain | Residue | Details |
A | SER283-GLU302 |
Functional Information from SwissProt/UniProt
site_id | SWS_FT_FI1 |
Number of Residues | 36 |
Details | BINDING: BINDING => ECO:0000269|PubMed:12704186, ECO:0000269|PubMed:17088542, ECO:0000269|PubMed:8861937 |
Chain | Residue | Details |
A | GLU105 | |
B | GLU196 | |
B | GLU229 | |
B | HIS232 | |
C | GLU105 | |
C | GLU143 | |
C | HIS146 | |
C | GLU196 | |
C | GLU229 | |
C | HIS232 | |
D | GLU105 | |
A | GLU143 | |
D | GLU143 | |
D | HIS146 | |
D | GLU196 | |
D | GLU229 | |
D | HIS232 | |
E | GLU105 | |
E | GLU143 | |
E | HIS146 | |
E | GLU196 | |
E | GLU229 | |
A | HIS146 | |
E | HIS232 | |
F | GLU105 | |
F | GLU143 | |
F | HIS146 | |
F | GLU196 | |
F | GLU229 | |
F | HIS232 | |
A | GLU196 | |
A | GLU229 | |
A | HIS232 | |
B | GLU105 | |
B | GLU143 | |
B | HIS146 |
Catalytic Information from CSA
site_id | MCSA1 |
Number of Residues | 9 |
Details | M-CSA 136 |
Chain | Residue | Details |
A | TRP62 | hydrogen bond donor, single electron acceptor, single electron donor, single electron relay |
A | GLU105 | metal ligand |
A | GLU143 | metal ligand |
A | HIS146 | hydrogen bond donor, metal ligand, single electron acceptor, single electron donor, single electron relay |
A | GLU196 | metal ligand |
A | THR199 | electrostatic stabiliser, hydrogen bond donor |
A | ASP228 | hydrogen bond acceptor, single electron acceptor, single electron donor, single electron relay |
A | GLU229 | metal ligand |
A | HIS232 | metal ligand |
site_id | MCSA2 |
Number of Residues | 9 |
Details | M-CSA 136 |
Chain | Residue | Details |
B | TRP62 | hydrogen bond donor, single electron acceptor, single electron donor, single electron relay |
B | GLU105 | metal ligand |
B | GLU143 | metal ligand |
B | HIS146 | hydrogen bond donor, metal ligand, single electron acceptor, single electron donor, single electron relay |
B | GLU196 | metal ligand |
B | THR199 | electrostatic stabiliser, hydrogen bond donor |
B | ASP228 | hydrogen bond acceptor, single electron acceptor, single electron donor, single electron relay |
B | GLU229 | metal ligand |
B | HIS232 | metal ligand |
site_id | MCSA3 |
Number of Residues | 9 |
Details | M-CSA 136 |
Chain | Residue | Details |
C | TRP62 | hydrogen bond donor, single electron acceptor, single electron donor, single electron relay |
C | GLU105 | metal ligand |
C | GLU143 | metal ligand |
C | HIS146 | hydrogen bond donor, metal ligand, single electron acceptor, single electron donor, single electron relay |
C | GLU196 | metal ligand |
C | THR199 | electrostatic stabiliser, hydrogen bond donor |
C | ASP228 | hydrogen bond acceptor, single electron acceptor, single electron donor, single electron relay |
C | GLU229 | metal ligand |
C | HIS232 | metal ligand |
site_id | MCSA4 |
Number of Residues | 9 |
Details | M-CSA 136 |
Chain | Residue | Details |
D | TRP62 | hydrogen bond donor, single electron acceptor, single electron donor, single electron relay |
D | GLU105 | metal ligand |
D | GLU143 | metal ligand |
D | HIS146 | hydrogen bond donor, metal ligand, single electron acceptor, single electron donor, single electron relay |
D | GLU196 | metal ligand |
D | THR199 | electrostatic stabiliser, hydrogen bond donor |
D | ASP228 | hydrogen bond acceptor, single electron acceptor, single electron donor, single electron relay |
D | GLU229 | metal ligand |
D | HIS232 | metal ligand |
site_id | MCSA5 |
Number of Residues | 9 |
Details | M-CSA 136 |
Chain | Residue | Details |
E | TRP62 | hydrogen bond donor, single electron acceptor, single electron donor, single electron relay |
E | GLU105 | metal ligand |
E | GLU143 | metal ligand |
E | HIS146 | hydrogen bond donor, metal ligand, single electron acceptor, single electron donor, single electron relay |
E | GLU196 | metal ligand |
E | THR199 | electrostatic stabiliser, hydrogen bond donor |
E | ASP228 | hydrogen bond acceptor, single electron acceptor, single electron donor, single electron relay |
E | GLU229 | metal ligand |
E | HIS232 | metal ligand |
site_id | MCSA6 |
Number of Residues | 9 |
Details | M-CSA 136 |
Chain | Residue | Details |
F | TRP62 | hydrogen bond donor, single electron acceptor, single electron donor, single electron relay |
F | GLU105 | metal ligand |
F | GLU143 | metal ligand |
F | HIS146 | hydrogen bond donor, metal ligand, single electron acceptor, single electron donor, single electron relay |
F | GLU196 | metal ligand |
F | THR199 | electrostatic stabiliser, hydrogen bond donor |
F | ASP228 | hydrogen bond acceptor, single electron acceptor, single electron donor, single electron relay |
F | GLU229 | metal ligand |
F | HIS232 | metal ligand |