4U4W
Structure of a nitrate/nitrite antiporter NarK in nitrate-bound occluded state
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0005452 | molecular_function | solute:inorganic anion antiporter activity |
| A | 0005886 | cellular_component | plasma membrane |
| A | 0015112 | molecular_function | nitrate transmembrane transporter activity |
| A | 0015113 | molecular_function | nitrite transmembrane transporter activity |
| A | 0015706 | biological_process | nitrate transmembrane transport |
| A | 0015707 | biological_process | nitrite transport |
| A | 0016020 | cellular_component | membrane |
| A | 0022857 | molecular_function | transmembrane transporter activity |
| A | 0042128 | biological_process | nitrate assimilation |
| A | 0043602 | biological_process | nitrate catabolic process |
| A | 0055085 | biological_process | transmembrane transport |
| B | 0005452 | molecular_function | solute:inorganic anion antiporter activity |
| B | 0005886 | cellular_component | plasma membrane |
| B | 0015112 | molecular_function | nitrate transmembrane transporter activity |
| B | 0015113 | molecular_function | nitrite transmembrane transporter activity |
| B | 0015706 | biological_process | nitrate transmembrane transport |
| B | 0015707 | biological_process | nitrite transport |
| B | 0016020 | cellular_component | membrane |
| B | 0022857 | molecular_function | transmembrane transporter activity |
| B | 0042128 | biological_process | nitrate assimilation |
| B | 0043602 | biological_process | nitrate catabolic process |
| B | 0055085 | biological_process | transmembrane transport |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 8 |
| Details | binding site for residue NO3 A 501 |
| Chain | Residue |
| A | PHE49 |
| A | ARG89 |
| A | PHE147 |
| A | ASN175 |
| A | TYR263 |
| A | PHE267 |
| A | ARG305 |
| A | SER411 |
| site_id | AC2 |
| Number of Residues | 3 |
| Details | binding site for residue OLA A 502 |
| Chain | Residue |
| A | TYR92 |
| A | OLC509 |
| A | PRO91 |
| site_id | AC3 |
| Number of Residues | 4 |
| Details | binding site for residue OLA A 503 |
| Chain | Residue |
| A | PRO43 |
| A | LEU47 |
| A | ILE218 |
| A | PHE222 |
| site_id | AC4 |
| Number of Residues | 3 |
| Details | binding site for residue OLA A 504 |
| Chain | Residue |
| A | PHE121 |
| A | PHE197 |
| A | PRO221 |
| site_id | AC5 |
| Number of Residues | 5 |
| Details | binding site for residue OLA A 505 |
| Chain | Residue |
| A | LEU88 |
| A | SER131 |
| A | LEU138 |
| A | PHE142 |
| B | OLA502 |
| site_id | AC6 |
| Number of Residues | 4 |
| Details | binding site for residue OLA A 506 |
| Chain | Residue |
| A | PHE420 |
| A | ALA424 |
| A | GLY437 |
| B | LYS203 |
| site_id | AC7 |
| Number of Residues | 2 |
| Details | binding site for residue OLC A 507 |
| Chain | Residue |
| A | LEU183 |
| A | GLN292 |
| site_id | AC8 |
| Number of Residues | 8 |
| Details | binding site for residue OLC A 508 |
| Chain | Residue |
| A | PHE107 |
| A | GLY110 |
| A | ILE228 |
| A | TRP231 |
| A | PHE232 |
| A | OLC509 |
| B | ILE228 |
| B | PHE232 |
| site_id | AC9 |
| Number of Residues | 7 |
| Details | binding site for residue OLC A 509 |
| Chain | Residue |
| A | ILE98 |
| A | PHE99 |
| A | OLA502 |
| A | OLC508 |
| B | TRP39 |
| B | OLA505 |
| B | OLA506 |
| site_id | AD1 |
| Number of Residues | 6 |
| Details | binding site for residue NO3 B 501 |
| Chain | Residue |
| B | PHE49 |
| B | ARG89 |
| B | PHE147 |
| B | ASN175 |
| B | TYR263 |
| B | PHE267 |
| site_id | AD2 |
| Number of Residues | 5 |
| Details | binding site for residue OLA B 502 |
| Chain | Residue |
| A | ILE135 |
| A | OLA505 |
| B | LEU187 |
| B | LEU191 |
| B | OLA503 |
| site_id | AD3 |
| Number of Residues | 4 |
| Details | binding site for residue OLA B 503 |
| Chain | Residue |
| B | LEU47 |
| B | TRP219 |
| B | PHE222 |
| B | OLA502 |
| site_id | AD4 |
| Number of Residues | 3 |
| Details | binding site for residue OLA B 504 |
| Chain | Residue |
| B | PHE194 |
| B | PHE197 |
| B | TRP217 |
| site_id | AD5 |
| Number of Residues | 4 |
| Details | binding site for residue OLA B 505 |
| Chain | Residue |
| A | OLC509 |
| B | SER35 |
| B | TRP39 |
| B | ALA166 |
| site_id | AD6 |
| Number of Residues | 2 |
| Details | binding site for residue OLA B 506 |
| Chain | Residue |
| A | OLC509 |
| B | SER306 |
| site_id | AD7 |
| Number of Residues | 15 |
| Details | binding site for residue OLC B 507 |
| Chain | Residue |
| A | ILE111 |
| A | TRP118 |
| A | PHE121 |
| A | ASP125 |
| A | LEU139 |
| A | PHE142 |
| B | SER192 |
| B | ILE193 |
| B | PHE194 |
| B | ALA195 |
| B | VAL196 |
| B | ILE218 |
| B | PHE222 |
| B | ILE225 |
| B | HOH645 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 218 |
| Details | TOPO_DOM: Cytoplasmic => ECO:0000269|PubMed:25959928, ECO:0007744|PDB:4U4T, ECO:0007744|PDB:4U4V |
| Chain | Residue | Details |
| A | MET1-ASN37 | |
| B | MET234-ARG253 | |
| B | ASP313-GLY316 | |
| B | SER374-ALA405 | |
| A | VAL96-ARG102 | |
| A | ALA152-ALA166 | |
| A | MET234-ARG253 | |
| A | ASP313-GLY316 | |
| A | SER374-ALA405 | |
| B | MET1-ASN37 | |
| B | VAL96-ARG102 | |
| B | ALA152-ALA166 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 524 |
| Details | TRANSMEM: Helical => ECO:0000269|PubMed:25959928, ECO:0007744|PDB:4U4T, ECO:0007744|PDB:4U4V |
| Chain | Residue | Details |
| A | LEU38-ALA59 | |
| A | PHE348-ILE373 | |
| A | ALA406-SER427 | |
| A | VAL436-GLY458 | |
| B | LEU38-ALA59 | |
| B | LEU74-MET95 | |
| B | ARG103-ALA122 | |
| B | SER131-MET151 | |
| B | LEU167-VAL189 | |
| B | LEU212-GLY233 | |
| B | GLY254-SER281 | |
| A | LEU74-MET95 | |
| B | ILE290-SER312 | |
| B | GLY317-THR338 | |
| B | PHE348-ILE373 | |
| B | ALA406-SER427 | |
| B | VAL436-GLY458 | |
| A | ARG103-ALA122 | |
| A | SER131-MET151 | |
| A | LEU167-VAL189 | |
| A | LEU212-GLY233 | |
| A | GLY254-SER281 | |
| A | ILE290-SER312 | |
| A | GLY317-THR338 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 126 |
| Details | TOPO_DOM: Periplasmic => ECO:0000269|PubMed:25959928, ECO:0007744|PDB:4U4T, ECO:0007744|PDB:4U4V |
| Chain | Residue | Details |
| A | VAL60-GLN73 | |
| B | LYS282-GLN289 | |
| B | LEU339-SER347 | |
| B | SER428-PRO435 | |
| A | VAL123-TYR130 | |
| A | SER190-TYR211 | |
| A | LYS282-GLN289 | |
| A | LEU339-SER347 | |
| A | SER428-PRO435 | |
| B | VAL60-GLN73 | |
| B | VAL123-TYR130 | |
| B | SER190-TYR211 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 8 |
| Details | TOPO_DOM: Cytoplasmic => ECO:0000269|PubMed:15919996, ECO:0000269|PubMed:25959928, ECO:0007744|PDB:4U4T, ECO:0007744|PDB:4U4V |
| Chain | Residue | Details |
| A | ARG459-LYS463 | |
| B | ARG459-LYS463 |
| site_id | SWS_FT_FI5 |
| Number of Residues | 4 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:23665960, ECO:0007744|PDB:4JRE |
| Chain | Residue | Details |
| A | ARG89 | |
| A | TYR263 | |
| B | ARG89 | |
| B | TYR263 |
| site_id | SWS_FT_FI6 |
| Number of Residues | 2 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:25959928, ECO:0007744|PDB:4U4T, ECO:0007744|PDB:4U4W |
| Chain | Residue | Details |
| A | ASN175 | |
| B | ASN175 |
| site_id | SWS_FT_FI7 |
| Number of Residues | 2 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:25959928, ECO:0007744|PDB:4U4T |
| Chain | Residue | Details |
| A | SER411 | |
| B | SER411 |
| site_id | SWS_FT_FI8 |
| Number of Residues | 2 |
| Details | SITE: Important for activity => ECO:0000305|PubMed:23665960, ECO:0000305|PubMed:25959928 |
| Chain | Residue | Details |
| A | ARG305 | |
| B | ARG305 |
