4U4W

Structure of a nitrate/nitrite antiporter NarK in nitrate-bound occluded state

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0005452	molecular_function	solute:inorganic anion antiporter activity
A	0005886	cellular_component	plasma membrane
A	0015112	molecular_function	nitrate transmembrane transporter activity
A	0015113	molecular_function	nitrite transmembrane transporter activity
A	0015291	molecular_function	secondary active transmembrane transporter activity
A	0015706	biological_process	nitrate transmembrane transport
A	0015707	biological_process	nitrite transport
A	0016020	cellular_component	membrane
A	0022857	molecular_function	transmembrane transporter activity
A	0042128	biological_process	nitrate assimilation
A	0043602	biological_process	nitrate catabolic process
A	0055085	biological_process	transmembrane transport
B	0005452	molecular_function	solute:inorganic anion antiporter activity
B	0005886	cellular_component	plasma membrane
B	0015112	molecular_function	nitrate transmembrane transporter activity
B	0015113	molecular_function	nitrite transmembrane transporter activity
B	0015291	molecular_function	secondary active transmembrane transporter activity
B	0015706	biological_process	nitrate transmembrane transport
B	0015707	biological_process	nitrite transport
B	0016020	cellular_component	membrane
B	0022857	molecular_function	transmembrane transporter activity
B	0042128	biological_process	nitrate assimilation
B	0043602	biological_process	nitrate catabolic process
B	0055085	biological_process	transmembrane transport

Functional Information from PDB Data

site_id	AC1
Number of Residues	8
Details	binding site for residue NO3 A 501

Chain	Residue
A	PHE49
A	ARG89
A	PHE147
A	ASN175
A	TYR263
A	PHE267
A	ARG305
A	SER411

---

site_id	AC2
Number of Residues	3
Details	binding site for residue OLA A 502

Chain	Residue
A	TYR92
A	OLC509
A	PRO91

---

site_id	AC3
Number of Residues	4
Details	binding site for residue OLA A 503

Chain	Residue
A	PRO43
A	LEU47
A	ILE218
A	PHE222

---

site_id	AC4
Number of Residues	3
Details	binding site for residue OLA A 504

Chain	Residue
A	PHE121
A	PHE197
A	PRO221

---

site_id	AC5
Number of Residues	5
Details	binding site for residue OLA A 505

Chain	Residue
A	LEU88
A	SER131
A	LEU138
A	PHE142
B	OLA502

---

site_id	AC6
Number of Residues	4
Details	binding site for residue OLA A 506

Chain	Residue
A	PHE420
A	ALA424
A	GLY437
B	LYS203

---

site_id	AC7
Number of Residues	2
Details	binding site for residue OLC A 507

Chain	Residue
A	LEU183
A	GLN292

---

site_id	AC8
Number of Residues	8
Details	binding site for residue OLC A 508

Chain	Residue
A	PHE107
A	GLY110
A	ILE228
A	TRP231
A	PHE232
A	OLC509
B	ILE228
B	PHE232

---

site_id	AC9
Number of Residues	7
Details	binding site for residue OLC A 509

Chain	Residue
A	ILE98
A	PHE99
A	OLA502
A	OLC508
B	TRP39
B	OLA505
B	OLA506

---

site_id	AD1
Number of Residues	6
Details	binding site for residue NO3 B 501

Chain	Residue
B	PHE49
B	ARG89
B	PHE147
B	ASN175
B	TYR263
B	PHE267

---

site_id	AD2
Number of Residues	5
Details	binding site for residue OLA B 502

Chain	Residue
A	ILE135
A	OLA505
B	LEU187
B	LEU191
B	OLA503

---

site_id	AD3
Number of Residues	4
Details	binding site for residue OLA B 503

Chain	Residue
B	LEU47
B	TRP219
B	PHE222
B	OLA502

---

site_id	AD4
Number of Residues	3
Details	binding site for residue OLA B 504

Chain	Residue
B	PHE194
B	PHE197
B	TRP217

---

site_id	AD5
Number of Residues	4
Details	binding site for residue OLA B 505

Chain	Residue
A	OLC509
B	SER35
B	TRP39
B	ALA166

---

site_id	AD6
Number of Residues	2
Details	binding site for residue OLA B 506

Chain	Residue
A	OLC509
B	SER306

---

site_id	AD7
Number of Residues	15
Details	binding site for residue OLC B 507

Chain	Residue
A	ILE111
A	TRP118
A	PHE121
A	ASP125
A	LEU139
A	PHE142
B	SER192
B	ILE193
B	PHE194
B	ALA195
B	VAL196
B	ILE218
B	PHE222
B	ILE225
B	HOH645

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	502
Details	Transmembrane: {"description":"Helical","evidences":[{"source":"PubMed","id":"25959928","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"4U4T","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4U4V","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	118
Details	Topological domain: {"description":"Periplasmic","evidences":[{"source":"PubMed","id":"25959928","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"4U4T","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4U4V","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	108
Details	Topological domain: {"description":"Cytoplasmic","evidences":[{"source":"PubMed","id":"25959928","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"4U4T","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4U4V","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	4
Details	Binding site: {"evidences":[{"source":"PubMed","id":"23665960","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"4JRE","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	2
Details	Binding site: {"evidences":[{"source":"PubMed","id":"25959928","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"4U4T","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4U4W","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	2
Details	Binding site: {"evidences":[{"source":"PubMed","id":"25959928","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"4U4T","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	2
Details	Site: {"description":"Important for activity","evidences":[{"source":"PubMed","id":"23665960","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"25959928","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---