4RQK

Crystal structure of PDK1 in complex with ATP and the PIF-pocket ligand RS1

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004672	molecular_function	protein kinase activity
A	0004674	molecular_function	protein serine/threonine kinase activity
A	0005524	molecular_function	ATP binding
A	0006468	biological_process	protein phosphorylation

Functional Information from PDB Data

site_id	AC1
Number of Residues	11
Details	BINDING SITE FOR RESIDUE GOL A 401

Chain	Residue
A	ALA103
A	HIS351
A	HOH2109
A	THR104
A	SER105
A	HIS139
A	SER191
A	TRP347
A	GLU348
A	ASN349
A	LEU350

---

site_id	AC2
Number of Residues	6
Details	BINDING SITE FOR RESIDUE GOL A 402

Chain	Residue
A	PHE82
A	LYS83
A	PHE84
A	GLU194
A	GLY334
A	LYS337

---

site_id	AC3
Number of Residues	22
Details	BINDING SITE FOR RESIDUE ATP A 403

Chain	Residue
A	GLY89
A	GLY91
A	SER92
A	SER94
A	VAL96
A	ALA109
A	LYS111
A	SER160
A	ALA162
A	GLU166
A	LEU212
A	HOH2030
A	HOH2089
A	HOH2111
A	HOH2113
A	HOH2146
A	HOH2153
A	HOH2185
A	HOH2186
A	HOH2218
A	HOH2221
A	HOH2266

---

site_id	AC4
Number of Residues	10
Details	BINDING SITE FOR RESIDUE R1S A 404

Chain	Residue
A	LYS115
A	ILE118
A	ILE119
A	VAL127
A	ARG131
A	THR148
A	PHE149
A	TYR156
A	PHE157
A	HOH2152

---

site_id	AC5
Number of Residues	12
Details	BINDING SITE FOR RESIDUE EPE A 405

Chain	Residue
A	ARG172
A	GLY175
A	LEU254
A	LYS257
A	GLN274
A	GLY278
A	LEU279
A	PRO280
A	GLY288
A	PHE291
A	ALA292
A	HOH2091

---

Functional Information from PROSITE/UniProt

site_id	PS00107
Number of Residues	24
Details	PROTEIN_KINASE_ATP Protein kinases ATP-binding region signature. LGEGSFSTVVlArelatsre..........YAIK

Chain	Residue	Details
A	LEU88-LYS111

---

site_id	PS00108
Number of Residues	13
Details	PROTEIN_KINASE_ST Serine/Threonine protein kinases active-site signature. IiHrDLKpeNILL

Chain	Residue	Details
A	ILE201-LEU213

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	44
Details	Region: {"description":"PIF-pocket","evidences":[{"source":"PubMed","id":"22999883","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	1
Details	Active site: {"description":"Proton acceptor","evidences":[{"source":"PROSITE-ProRule","id":"PRU00159","evidenceCode":"ECO:0000255"},{"source":"PROSITE-ProRule","id":"PRU10027","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	7
Details	Binding site: {"evidences":[{"source":"PubMed","id":"12169624","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"15741170","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"22999883","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	1
Details	Binding site: {"evidences":[{"source":"PubMed","id":"22999883","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	1
Details	Modified residue: {"description":"Phosphoserine; by autocatalysis","evidences":[{"source":"PubMed","id":"10455013","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"11481331","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"15772071","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"16780920","evidenceCode":"ECO:0000269"},{"source":"Reference","evidenceCode":"ECO:0000269","citation":{"citationType":"submission","publicationDate":"MAR-2009","submissionDatabase":"UniProtKB","authors":["Bienvenut W.V.","Waridel P.","Quadroni M."]}}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	1
Details	Modified residue: {"description":"N6-acetyllysine","evidences":[{"source":"UniProtKB","id":"Q9Z2A0","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	1
Details	Modified residue: {"description":"Phosphothreonine; by MELK","evidences":[{"source":"PubMed","id":"22544756","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---