4RJK
Acetolactate synthase from Bacillus subtilis bound to LThDP - crystal form II
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0000287 | molecular_function | magnesium ion binding |
| A | 0003824 | molecular_function | catalytic activity |
| A | 0003984 | molecular_function | acetolactate synthase activity |
| A | 0030976 | molecular_function | thiamine pyrophosphate binding |
| A | 0034077 | biological_process | butanediol metabolic process |
| B | 0000287 | molecular_function | magnesium ion binding |
| B | 0003824 | molecular_function | catalytic activity |
| B | 0003984 | molecular_function | acetolactate synthase activity |
| B | 0030976 | molecular_function | thiamine pyrophosphate binding |
| B | 0034077 | biological_process | butanediol metabolic process |
| C | 0000287 | molecular_function | magnesium ion binding |
| C | 0003824 | molecular_function | catalytic activity |
| C | 0003984 | molecular_function | acetolactate synthase activity |
| C | 0030976 | molecular_function | thiamine pyrophosphate binding |
| C | 0034077 | biological_process | butanediol metabolic process |
| D | 0000287 | molecular_function | magnesium ion binding |
| D | 0003824 | molecular_function | catalytic activity |
| D | 0003984 | molecular_function | acetolactate synthase activity |
| D | 0030976 | molecular_function | thiamine pyrophosphate binding |
| D | 0034077 | biological_process | butanediol metabolic process |
| E | 0000287 | molecular_function | magnesium ion binding |
| E | 0003824 | molecular_function | catalytic activity |
| E | 0003984 | molecular_function | acetolactate synthase activity |
| E | 0030976 | molecular_function | thiamine pyrophosphate binding |
| E | 0034077 | biological_process | butanediol metabolic process |
| F | 0000287 | molecular_function | magnesium ion binding |
| F | 0003824 | molecular_function | catalytic activity |
| F | 0003984 | molecular_function | acetolactate synthase activity |
| F | 0030976 | molecular_function | thiamine pyrophosphate binding |
| F | 0034077 | biological_process | butanediol metabolic process |
| G | 0000287 | molecular_function | magnesium ion binding |
| G | 0003824 | molecular_function | catalytic activity |
| G | 0003984 | molecular_function | acetolactate synthase activity |
| G | 0030976 | molecular_function | thiamine pyrophosphate binding |
| G | 0034077 | biological_process | butanediol metabolic process |
| H | 0000287 | molecular_function | magnesium ion binding |
| H | 0003824 | molecular_function | catalytic activity |
| H | 0003984 | molecular_function | acetolactate synthase activity |
| H | 0030976 | molecular_function | thiamine pyrophosphate binding |
| H | 0034077 | biological_process | butanediol metabolic process |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 25 |
| Details | BINDING SITE FOR RESIDUE TPP A 601 |
| Chain | Residue |
| A | ILE398 |
| A | GLY453 |
| A | ASP478 |
| A | THR480 |
| A | TYR481 |
| A | ASP482 |
| A | MET483 |
| A | VAL484 |
| A | TYR547 |
| A | MG602 |
| A | HOH780 |
| A | GLY399 |
| A | HOH791 |
| B | PRO37 |
| B | GLU61 |
| B | PRO87 |
| B | ASN91 |
| B | GLN124 |
| A | SER400 |
| A | HIS401 |
| A | GLN424 |
| A | LEU426 |
| A | GLY450 |
| A | ASP451 |
| A | GLY452 |
| site_id | AC2 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE MG A 602 |
| Chain | Residue |
| A | ASP451 |
| A | ASP478 |
| A | THR480 |
| A | TPP601 |
| A | HOH791 |
| site_id | AC3 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE PG4 A 603 |
| Chain | Residue |
| A | ARG73 |
| A | THR75 |
| A | GLY76 |
| A | ARG224 |
| A | PRO225 |
| site_id | AC4 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE PG4 A 604 |
| Chain | Residue |
| A | ASN386 |
| A | ASP389 |
| A | ASP390 |
| H | ALA203 |
| H | LYS207 |
| H | THR210 |
| H | THR304 |
| site_id | AC5 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE PG4 A 605 |
| Chain | Residue |
| A | ASN100 |
| A | THR101 |
| A | GLN163 |
| A | ASP314 |
| A | PG4608 |
| B | ARG121 |
| site_id | AC6 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE PG4 A 606 |
| Chain | Residue |
| A | HIS391 |
| A | THR393 |
| A | LEU415 |
| A | LYS440 |
| A | GLU443 |
| site_id | AC7 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE PG4 A 607 |
| Chain | Residue |
| A | ALA161 |
| A | GLY162 |
| A | HOH759 |
| D | GLY162 |
| D | PRO191 |
| D | ILE312 |
| site_id | AC8 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE PG4 A 608 |
| Chain | Residue |
| A | LEU97 |
| A | ASN100 |
| A | THR101 |
| A | PRO134 |
| A | PG4605 |
| B | ARG121 |
| B | SER125 |
| site_id | AC9 |
| Number of Residues | 24 |
| Details | BINDING SITE FOR RESIDUE TPP B 601 |
| Chain | Residue |
| A | PRO37 |
| A | GLU61 |
| A | PRO87 |
| A | ASN91 |
| A | GLN124 |
| B | ILE398 |
| B | GLY399 |
| B | SER400 |
| B | HIS401 |
| B | GLN424 |
| B | LEU426 |
| B | GLY450 |
| B | ASP451 |
| B | GLY452 |
| B | GLY453 |
| B | ASP478 |
| B | THR480 |
| B | TYR481 |
| B | ASP482 |
| B | MET483 |
| B | VAL484 |
| B | TYR547 |
| B | MG602 |
| B | HOH812 |
| site_id | BC1 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE MG B 602 |
| Chain | Residue |
| B | ASP451 |
| B | ASP478 |
| B | THR480 |
| B | TPP601 |
| B | HOH812 |
| site_id | BC2 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE PG4 B 603 |
| Chain | Residue |
| B | SER160 |
| B | ARG224 |
| B | HOH800 |
| B | GLY72 |
| B | ARG73 |
| B | GLY76 |
| B | PRO105 |
| site_id | BC3 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE PG4 B 604 |
| Chain | Residue |
| B | GLY442 |
| B | PRO470 |
| B | ALA535 |
| B | GLU536 |
| C | LYS233 |
| C | GLU337 |
| site_id | BC4 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE PG4 B 605 |
| Chain | Residue |
| B | SER331 |
| B | ASN334 |
| B | HIS335 |
| C | ASP198 |
| site_id | BC5 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE PG4 B 606 |
| Chain | Residue |
| B | LEU415 |
| B | VAL439 |
| B | LYS440 |
| B | GLU443 |
| site_id | BC6 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE PG4 B 607 |
| Chain | Residue |
| B | ALA203 |
| B | LYS207 |
| B | ASP322 |
| site_id | BC7 |
| Number of Residues | 25 |
| Details | BINDING SITE FOR RESIDUE TPP C 601 |
| Chain | Residue |
| C | ILE398 |
| C | GLY399 |
| C | SER400 |
| C | HIS401 |
| C | GLN424 |
| C | LEU426 |
| C | GLY450 |
| C | ASP451 |
| C | GLY452 |
| C | GLY453 |
| C | PHE456 |
| C | ASP478 |
| C | THR480 |
| C | TYR481 |
| C | ASP482 |
| C | MET483 |
| C | VAL484 |
| C | TYR547 |
| C | MG602 |
| C | HOH817 |
| D | PRO37 |
| D | GLU61 |
| D | PRO87 |
| D | ASN91 |
| D | GLN124 |
| site_id | BC8 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE MG C 602 |
| Chain | Residue |
| C | ASP451 |
| C | ASP478 |
| C | THR480 |
| C | TPP601 |
| C | HOH817 |
| site_id | BC9 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE PG4 C 603 |
| Chain | Residue |
| C | LYS207 |
| C | THR304 |
| site_id | CC1 |
| Number of Residues | 8 |
| Details | BINDING SITE FOR RESIDUE PG4 C 604 |
| Chain | Residue |
| C | LEU74 |
| C | HIS391 |
| C | PRO414 |
| C | LEU415 |
| C | THR416 |
| C | VAL439 |
| C | LYS440 |
| C | GLU443 |
| site_id | CC2 |
| Number of Residues | 8 |
| Details | BINDING SITE FOR RESIDUE PG4 C 605 |
| Chain | Residue |
| C | GLY72 |
| C | ARG73 |
| C | THR75 |
| C | GLY76 |
| C | PRO105 |
| C | SER160 |
| C | PRO225 |
| C | HOH807 |
| site_id | CC3 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE PG4 C 606 |
| Chain | Residue |
| B | LYS233 |
| B | GLU337 |
| C | LYS444 |
| C | ALA535 |
| C | GLU536 |
| site_id | CC4 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE PG4 C 607 |
| Chain | Residue |
| C | ARG121 |
| D | ASN100 |
| site_id | CC5 |
| Number of Residues | 23 |
| Details | BINDING SITE FOR RESIDUE TPP D 601 |
| Chain | Residue |
| C | PRO37 |
| C | GLU61 |
| C | PRO87 |
| C | ASN91 |
| D | ILE398 |
| D | GLY399 |
| D | SER400 |
| D | HIS401 |
| D | GLN424 |
| D | LEU426 |
| D | GLY450 |
| D | ASP451 |
| D | GLY452 |
| D | GLY453 |
| D | ASP478 |
| D | THR480 |
| D | TYR481 |
| D | ASP482 |
| D | MET483 |
| D | VAL484 |
| D | TYR547 |
| D | MG602 |
| D | HOH707 |
| site_id | CC6 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE MG D 602 |
| Chain | Residue |
| D | ASP451 |
| D | ASP478 |
| D | THR480 |
| D | TPP601 |
| D | HOH707 |
| site_id | CC7 |
| Number of Residues | 9 |
| Details | BINDING SITE FOR RESIDUE PG4 D 603 |
| Chain | Residue |
| D | ARG254 |
| D | VAL388 |
| D | ASP389 |
| D | ASP390 |
| D | PG4609 |
| E | ALA203 |
| E | LYS207 |
| E | THR304 |
| E | ASP322 |
| site_id | CC8 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE PG4 D 604 |
| Chain | Residue |
| D | GLU27 |
| D | HOH760 |
| E | GLY442 |
| E | LYS444 |
| E | PRO470 |
| E | ASN534 |
| E | PG4608 |
| site_id | CC9 |
| Number of Residues | 8 |
| Details | BINDING SITE FOR RESIDUE PG4 D 605 |
| Chain | Residue |
| D | LYS207 |
| D | THR210 |
| D | THR304 |
| H | ASN386 |
| H | ALA387 |
| H | ASP389 |
| H | ASP390 |
| H | ARG530 |
| site_id | DC1 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE PG4 D 606 |
| Chain | Residue |
| D | LEU74 |
| D | VAL439 |
| D | LYS440 |
| D | GLU443 |
| site_id | DC2 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE PG4 D 607 |
| Chain | Residue |
| D | GLY72 |
| D | ARG73 |
| D | GLY76 |
| D | SER160 |
| D | ALA164 |
| D | ARG224 |
| D | PRO225 |
| site_id | DC3 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE PG4 D 608 |
| Chain | Residue |
| D | ASP389 |
| D | GLU443 |
| D | LYS444 |
| E | ARG186 |
| E | ASP322 |
| site_id | DC4 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE PG4 D 609 |
| Chain | Residue |
| D | ASP390 |
| D | HIS391 |
| D | GLU413 |
| D | PG4603 |
| E | ASP199 |
| E | HOH786 |
| site_id | DC5 |
| Number of Residues | 24 |
| Details | BINDING SITE FOR RESIDUE TPP E 601 |
| Chain | Residue |
| E | ILE398 |
| E | GLY399 |
| E | SER400 |
| E | HIS401 |
| E | GLN424 |
| E | LEU426 |
| E | GLY450 |
| E | ASP451 |
| E | GLY452 |
| E | GLY453 |
| E | ASP478 |
| E | THR480 |
| E | TYR481 |
| E | ASP482 |
| E | MET483 |
| E | VAL484 |
| E | TYR547 |
| E | MG602 |
| E | HOH750 |
| F | PRO37 |
| F | GLU61 |
| F | PRO87 |
| F | ASN91 |
| F | GLN124 |
| site_id | DC6 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE MG E 602 |
| Chain | Residue |
| E | ASP451 |
| E | ASP478 |
| E | THR480 |
| E | TPP601 |
| E | HOH750 |
| site_id | DC7 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE PG4 E 603 |
| Chain | Residue |
| A | LYS207 |
| A | THR304 |
| A | ASP322 |
| E | ASN386 |
| E | VAL388 |
| E | ASP389 |
| E | ASP390 |
| site_id | DC8 |
| Number of Residues | 8 |
| Details | BINDING SITE FOR RESIDUE PG4 E 604 |
| Chain | Residue |
| D | GLY442 |
| D | LYS444 |
| D | PRO470 |
| D | ASN534 |
| D | ALA535 |
| D | GLU536 |
| E | GLU27 |
| E | ASN184 |
| site_id | DC9 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE PG4 E 605 |
| Chain | Residue |
| E | LEU74 |
| E | LEU415 |
| E | VAL439 |
| E | LYS440 |
| site_id | EC1 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE PG4 E 606 |
| Chain | Residue |
| E | ASN100 |
| E | THR101 |
| E | ASP288 |
| E | ILE290 |
| E | ASP314 |
| F | ARG121 |
| site_id | EC2 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE PG4 E 607 |
| Chain | Residue |
| E | GLU364 |
| E | LYS382 |
| E | TYR408 |
| site_id | EC3 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE PG4 E 608 |
| Chain | Residue |
| A | ASP322 |
| D | ARG186 |
| D | PG4604 |
| E | ASP389 |
| E | GLU443 |
| E | LYS444 |
| site_id | EC4 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE PG4 E 609 |
| Chain | Residue |
| E | GLY72 |
| E | ARG73 |
| E | GLY76 |
| E | PRO105 |
| E | ALA164 |
| E | PRO225 |
| E | HOH800 |
| site_id | EC5 |
| Number of Residues | 22 |
| Details | BINDING SITE FOR RESIDUE TPP F 601 |
| Chain | Residue |
| E | PRO37 |
| E | GLU61 |
| E | ASN91 |
| F | GLY399 |
| F | SER400 |
| F | HIS401 |
| F | GLN424 |
| F | LEU426 |
| F | GLY450 |
| F | ASP451 |
| F | GLY452 |
| F | GLY453 |
| F | ASP478 |
| F | THR480 |
| F | TYR481 |
| F | ASP482 |
| F | MET483 |
| F | VAL484 |
| F | TYR547 |
| F | MG602 |
| F | HOH753 |
| F | HOH819 |
| site_id | EC6 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE MG F 602 |
| Chain | Residue |
| F | ASP451 |
| F | ASP478 |
| F | THR480 |
| F | TPP601 |
| F | HOH819 |
| site_id | EC7 |
| Number of Residues | 8 |
| Details | BINDING SITE FOR RESIDUE PG4 F 603 |
| Chain | Residue |
| F | ARG73 |
| F | LEU74 |
| F | THR75 |
| F | GLY76 |
| F | ARG224 |
| F | PRO225 |
| F | PG4606 |
| F | HOH770 |
| site_id | EC8 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE PG4 F 604 |
| Chain | Residue |
| F | LYS233 |
| F | GLU337 |
| G | ALA535 |
| G | GLU536 |
| site_id | EC9 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE PG4 F 605 |
| Chain | Residue |
| F | ASN534 |
| F | ALA535 |
| F | GLU536 |
| G | LYS233 |
| G | LYS237 |
| G | GLU337 |
| site_id | FC1 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE PG4 F 606 |
| Chain | Residue |
| F | THR393 |
| F | LEU415 |
| F | VAL439 |
| F | LYS440 |
| F | PG4603 |
| site_id | FC2 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE PG4 F 607 |
| Chain | Residue |
| F | ALA161 |
| F | GLY162 |
| F | PRO191 |
| F | LYS192 |
| F | LEU193 |
| F | ASP328 |
| site_id | FC3 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE PG4 F 608 |
| Chain | Residue |
| F | LYS207 |
| F | ASP322 |
| site_id | FC4 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE PYR F 609 |
| Chain | Residue |
| E | GLN487 |
| E | LYS491 |
| F | ALA39 |
| F | LYS40 |
| F | GLN173 |
| site_id | FC5 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE PYR F 610 |
| Chain | Residue |
| F | SER437 |
| F | HOH778 |
| site_id | FC6 |
| Number of Residues | 23 |
| Details | BINDING SITE FOR RESIDUE TPP G 601 |
| Chain | Residue |
| G | ILE398 |
| G | GLY399 |
| G | SER400 |
| G | HIS401 |
| G | GLN424 |
| G | LEU426 |
| G | GLY450 |
| G | ASP451 |
| G | GLY452 |
| G | GLY453 |
| G | ASP478 |
| G | THR480 |
| G | TYR481 |
| G | ASP482 |
| G | MET483 |
| G | VAL484 |
| G | TYR547 |
| G | MG602 |
| G | HOH738 |
| H | PRO37 |
| H | GLU61 |
| H | PRO87 |
| H | ASN91 |
| site_id | FC7 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE MG G 602 |
| Chain | Residue |
| G | ASP451 |
| G | ASP478 |
| G | THR480 |
| G | TPP601 |
| G | HOH738 |
| site_id | FC8 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE PG4 G 603 |
| Chain | Residue |
| G | ARG73 |
| G | GLY76 |
| G | PRO105 |
| G | SER160 |
| G | ARG224 |
| site_id | FC9 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE PG4 G 604 |
| Chain | Residue |
| G | LYS207 |
| G | THR304 |
| G | ASP322 |
| site_id | GC1 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE PG4 G 605 |
| Chain | Residue |
| G | LEU74 |
| G | LEU415 |
| G | LYS440 |
| site_id | GC2 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE PG4 G 606 |
| Chain | Residue |
| G | GLU364 |
| G | TYR408 |
| site_id | GC3 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE PYR G 607 |
| Chain | Residue |
| F | ASP527 |
| F | ASN534 |
| G | GLN239 |
| G | ASP258 |
| G | GLN259 |
| site_id | GC4 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE PYR G 608 |
| Chain | Residue |
| G | GLY363 |
| G | GLN365 |
| G | ASP549 |
| G | ASN552 |
| site_id | GC5 |
| Number of Residues | 22 |
| Details | BINDING SITE FOR RESIDUE TDL H 601 |
| Chain | Residue |
| G | PRO37 |
| G | GLU61 |
| G | ASN91 |
| G | GLN124 |
| H | ILE398 |
| H | GLY399 |
| H | SER400 |
| H | HIS401 |
| H | GLN424 |
| H | LEU426 |
| H | GLY450 |
| H | ASP451 |
| H | GLY452 |
| H | GLY453 |
| H | ASP478 |
| H | THR480 |
| H | ASP482 |
| H | MET483 |
| H | VAL484 |
| H | TYR547 |
| H | MG602 |
| H | HOH760 |
| site_id | GC6 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE MG H 602 |
| Chain | Residue |
| H | ASP451 |
| H | ASP478 |
| H | THR480 |
| H | TDL601 |
| H | HOH760 |
| site_id | GC7 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE PG4 H 603 |
| Chain | Residue |
| H | ASN100 |
| H | PRO134 |
| site_id | GC8 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE PG4 H 604 |
| Chain | Residue |
| H | GLY72 |
| H | ARG73 |
| H | GLY76 |
| H | SER160 |
| H | ALA164 |
| H | ARG224 |
| H | PRO225 |
| site_id | GC9 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE PYR H 605 |
| Chain | Residue |
| A | HIS338 |
| H | LYS237 |
| H | GLU337 |
Functional Information from PROSITE/UniProt
| site_id | PS00187 |
| Number of Residues | 20 |
| Details | TPP_ENZYMES Thiamine pyrophosphate enzymes signature. IGaslvkPgekvVsVsGDGG |
| Chain | Residue | Details |
| A | ILE434-GLY453 |
