4R43

Crystal Structure Analysis of MTB PEPCK

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000166	molecular_function	nucleotide binding
A	0004611	molecular_function	phosphoenolpyruvate carboxykinase activity
A	0004613	molecular_function	phosphoenolpyruvate carboxykinase (GTP) activity
A	0005525	molecular_function	GTP binding
A	0005576	cellular_component	extracellular region
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0005886	cellular_component	plasma membrane
A	0006094	biological_process	gluconeogenesis
A	0006107	biological_process	oxaloacetate metabolic process
A	0009274	cellular_component	peptidoglycan-based cell wall
A	0010106	biological_process	cellular response to iron ion starvation
A	0016829	molecular_function	lyase activity
A	0016831	molecular_function	carboxy-lyase activity
A	0017076	molecular_function	purine nucleotide binding
A	0019543	biological_process	propionate catabolic process
A	0030145	molecular_function	manganese ion binding
A	0032869	biological_process	cellular response to insulin stimulus
A	0033993	biological_process	response to lipid
A	0042594	biological_process	response to starvation
A	0046327	biological_process	glycerol biosynthetic process from pyruvate
A	0046872	molecular_function	metal ion binding
A	0052572	biological_process	response to host immune response
A	0071333	biological_process	cellular response to glucose stimulus
A	0071549	biological_process	cellular response to dexamethasone stimulus

Functional Information from PDB Data

site_id	AC1
Number of Residues	22
Details	BINDING SITE FOR RESIDUE GDP A 701

Chain	Residue
A	PRO270
A	ARG377
A	ARG420
A	TRP501
A	PHE502
A	PHE510
A	GLY514
A	PHE515
A	ASN518
A	MN702
A	HOH812
A	ALA272
A	HOH819
A	HOH821
A	HOH841
A	CYS273
A	GLY274
A	LYS275
A	THR276
A	ASN277
A	ASP362
A	PHE376

---

site_id	AC2
Number of Residues	6
Details	BINDING SITE FOR RESIDUE MN A 702

Chain	Residue
A	THR276
A	GDP701
A	HOH819
A	HOH820
A	HOH821
A	HOH822

---

site_id	AC3
Number of Residues	3
Details	BINDING SITE FOR RESIDUE PEG A 703

Chain	Residue
A	ARG301
A	TYR309
A	PRO407

---

site_id	AC4
Number of Residues	3
Details	BINDING SITE FOR RESIDUE PEG A 704

Chain	Residue
A	ARG307
A	ILE412
A	SER413

---

site_id	AC5
Number of Residues	3
Details	BINDING SITE FOR RESIDUE PEG A 705

Chain	Residue
A	ARG157
A	ASN340
A	HOH829

---

site_id	AC6
Number of Residues	5
Details	BINDING SITE FOR RESIDUE PEG A 706

Chain	Residue
A	PRO283
A	THR284
A	ILE285
A	GLY287
A	TRP288

---

site_id	AC7
Number of Residues	7
Details	BINDING SITE FOR RESIDUE PO4 A 707

Chain	Residue
A	LYS229
A	HIS249
A	SER271
A	LYS275
A	ASP296
A	HOH820
A	HOH821

---

Functional Information from PROSITE/UniProt

site_id	PS00505
Number of Residues	9
Details	PEPCK_GTP Phosphoenolpyruvate carboxykinase (GTP) signature. FPSACGKTN

Chain	Residue	Details
A	PHE269-ASN277

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1
Details	ACT_SITE: ACT_SITE => ECO:0000255|HAMAP-Rule:MF_00452

Chain	Residue	Details
A	CYS273

---

site_id	SWS_FT_FI2
Number of Residues	11
Details	BINDING: BINDING => ECO:0000255|HAMAP-Rule:MF_00452

Chain	Residue	Details
A	ARG81
A	ARG420
A	PHE515
A	TYR220
A	LYS229
A	HIS249
A	SER271
A	ALA272
A	ASP296
A	ASN387
A	ARG389

---

site_id	SWS_FT_FI3
Number of Residues	1
Details	MOD_RES: N-acetylthreonine => ECO:0007744|PubMed:21969609

Chain	Residue	Details
A	THR2

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