4PL7
Structure of Komagataella pastoris actin-thymosin beta4 hybrid
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0003723 | molecular_function | RNA binding |
| A | 0003779 | molecular_function | actin binding |
| A | 0003785 | molecular_function | actin monomer binding |
| A | 0005515 | molecular_function | protein binding |
| A | 0005524 | molecular_function | ATP binding |
| A | 0005576 | cellular_component | extracellular region |
| A | 0005634 | cellular_component | nucleus |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0005829 | cellular_component | cytosol |
| A | 0005856 | cellular_component | cytoskeleton |
| A | 0007015 | biological_process | actin filament organization |
| A | 0016787 | molecular_function | hydrolase activity |
| A | 0019899 | molecular_function | enzyme binding |
| A | 0030334 | biological_process | regulation of cell migration |
| A | 0031093 | cellular_component | platelet alpha granule lumen |
| A | 0033209 | biological_process | tumor necrosis factor-mediated signaling pathway |
| A | 0042989 | biological_process | sequestering of actin monomers |
| A | 0043124 | biological_process | negative regulation of canonical NF-kappaB signal transduction |
| A | 0043536 | biological_process | positive regulation of blood vessel endothelial cell migration |
| A | 0050728 | biological_process | negative regulation of inflammatory response |
| A | 0140311 | molecular_function | protein sequestering activity |
| A | 1905273 | biological_process | positive regulation of proton-transporting ATP synthase activity, rotational mechanism |
| A | 2001028 | biological_process | positive regulation of endothelial cell chemotaxis |
| A | 2001171 | biological_process | positive regulation of ATP biosynthetic process |
| B | 0003723 | molecular_function | RNA binding |
| B | 0003779 | molecular_function | actin binding |
| B | 0003785 | molecular_function | actin monomer binding |
| B | 0005515 | molecular_function | protein binding |
| B | 0005524 | molecular_function | ATP binding |
| B | 0005576 | cellular_component | extracellular region |
| B | 0005634 | cellular_component | nucleus |
| B | 0005737 | cellular_component | cytoplasm |
| B | 0005829 | cellular_component | cytosol |
| B | 0005856 | cellular_component | cytoskeleton |
| B | 0007015 | biological_process | actin filament organization |
| B | 0016787 | molecular_function | hydrolase activity |
| B | 0019899 | molecular_function | enzyme binding |
| B | 0030334 | biological_process | regulation of cell migration |
| B | 0031093 | cellular_component | platelet alpha granule lumen |
| B | 0033209 | biological_process | tumor necrosis factor-mediated signaling pathway |
| B | 0042989 | biological_process | sequestering of actin monomers |
| B | 0043124 | biological_process | negative regulation of canonical NF-kappaB signal transduction |
| B | 0043536 | biological_process | positive regulation of blood vessel endothelial cell migration |
| B | 0050728 | biological_process | negative regulation of inflammatory response |
| B | 0140311 | molecular_function | protein sequestering activity |
| B | 1905273 | biological_process | positive regulation of proton-transporting ATP synthase activity, rotational mechanism |
| B | 2001028 | biological_process | positive regulation of endothelial cell chemotaxis |
| B | 2001171 | biological_process | positive regulation of ATP biosynthetic process |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 27 |
| Details | binding site for residue ATP A 501 |
| Chain | Residue |
| A | GLY13 |
| A | GLY182 |
| A | ARG210 |
| A | LYS213 |
| A | GLU214 |
| A | GLY301 |
| A | GLY302 |
| A | THR303 |
| A | MET305 |
| A | PHE306 |
| A | LYS336 |
| A | SER14 |
| A | CA502 |
| A | HOH611 |
| A | HOH625 |
| A | HOH626 |
| A | HOH628 |
| A | HOH634 |
| A | HOH635 |
| A | HOH686 |
| A | GLY15 |
| A | MET16 |
| A | LYS18 |
| A | GLY156 |
| A | ASP157 |
| A | GLY158 |
| A | VAL159 |
| site_id | AC2 |
| Number of Residues | 6 |
| Details | binding site for residue CA A 502 |
| Chain | Residue |
| A | ATP501 |
| A | HOH625 |
| A | HOH626 |
| A | HOH627 |
| A | HOH628 |
| A | HOH629 |
| site_id | AC3 |
| Number of Residues | 24 |
| Details | binding site for residue ATP B 501 |
| Chain | Residue |
| B | GLY13 |
| B | SER14 |
| B | GLY15 |
| B | MET16 |
| B | LYS18 |
| B | GLY156 |
| B | ASP157 |
| B | GLY158 |
| B | VAL159 |
| B | GLY182 |
| B | ARG210 |
| B | LYS213 |
| B | GLU214 |
| B | GLY302 |
| B | THR303 |
| B | MET305 |
| B | LYS336 |
| B | CA502 |
| B | HOH625 |
| B | HOH628 |
| B | HOH632 |
| B | HOH637 |
| B | HOH644 |
| B | HOH654 |
| site_id | AC4 |
| Number of Residues | 6 |
| Details | binding site for residue CA B 502 |
| Chain | Residue |
| B | ATP501 |
| B | HOH625 |
| B | HOH626 |
| B | HOH627 |
| B | HOH628 |
| B | HOH629 |
Functional Information from PROSITE/UniProt
| site_id | PS00406 |
| Number of Residues | 11 |
| Details | ACTINS_1 Actins signature 1. FVGDEAQs.KRG |
| Chain | Residue | Details |
| A | PHE53-GLY63 |
| site_id | PS00432 |
| Number of Residues | 9 |
| Details | ACTINS_2 Actins signature 2. WISKqEYDE |
| Chain | Residue | Details |
| A | TRP356-GLU364 |
| site_id | PS00500 |
| Number of Residues | 12 |
| Details | THYMOSIN_B4 Thymosin beta-4 family signature. LKKTETqeKNPL |
| Chain | Residue | Details |
| A | LEU406-LEU417 |
| site_id | PS01132 |
| Number of Residues | 13 |
| Details | ACTINS_ACT_LIKE Actins and actin-related proteins signature. LLTEApMNPksNR |
| Chain | Residue | Details |
| A | LEU104-ARG116 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 2 |
| Details | MOD_RES: Phosphoserine => ECO:0007744|PubMed:20068231, ECO:0007744|PubMed:21406692, ECO:0007744|PubMed:23186163 |
| Chain | Residue | Details |
| A | SER390 | |
| B | SER390 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 8 |
| Details | MOD_RES: N6-acetyllysine => ECO:0007744|PubMed:19608861 |
| Chain | Residue | Details |
| A | LYS392 | |
| A | LYS414 | |
| A | LYS420 | |
| A | LYS427 | |
| B | LYS392 | |
| B | LYS414 | |
| B | LYS420 | |
| B | LYS427 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 2 |
| Details | MOD_RES: N6-acetyllysine; alternate => ECO:0007744|PubMed:19608861 |
| Chain | Residue | Details |
| A | LYS400 | |
| B | LYS400 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 4 |
| Details | MOD_RES: Phosphothreonine => ECO:0007744|PubMed:23186163 |
| Chain | Residue | Details |
| A | THR411 | |
| A | THR422 | |
| B | THR411 | |
| B | THR422 |
| site_id | SWS_FT_FI5 |
| Number of Residues | 2 |
| Details | MOD_RES: Phosphoserine => ECO:0007744|PubMed:23186163 |
| Chain | Residue | Details |
| A | SER419 | |
| B | SER419 |
| site_id | SWS_FT_FI6 |
| Number of Residues | 4 |
| Details | CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternate => ECO:0007744|PubMed:28112733 |
| Chain | Residue | Details |
| A | LYS400 | |
| B | LYS400 |
