4PJK

Dicty myosin II R238E.E459R mutant (with ADP.Pi) in the Pi release state

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003774	molecular_function	cytoskeletal motor activity
A	0005524	molecular_function	ATP binding
A	0016459	cellular_component	myosin complex
A	0051015	molecular_function	actin filament binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	6
Details	binding site for residue MG A 801

Chain	Residue
A	THR186
A	SER237
A	ADP802
A	HOH911
A	HOH955
A	HOH902

---

site_id	AC2
Number of Residues	23
Details	binding site for residue ADP A 802

Chain	Residue
A	LYS130
A	TYR135
A	GLU180
A	GLY182
A	ALA183
A	GLY184
A	LYS185
A	THR186
A	GLU187
A	ASN233
A	MG801
A	PO4803
A	HOH911
A	HOH955
A	HOH902
A	HOH925
A	HOH1116
A	HOH1092
A	HOH1107
A	HOH1113
A	ASN127
A	PRO128
A	PHE129

---

site_id	AC3
Number of Residues	10
Details	binding site for residue PO4 A 803

Chain	Residue
A	GLU180
A	SER181
A	LYS185
A	SER237
A	SER456
A	GLY457
A	ARG459
A	ADP802
A	HOH902
A	HOH922

---

site_id	AC4
Number of Residues	7
Details	binding site for residue PO4 A 804

Chain	Residue
A	GLU180
A	ASN660
A	VAL671
A	ASP674
A	GLN675
A	GOL805
A	HOH1146

---

site_id	AC5
Number of Residues	9
Details	binding site for residue GOL A 805

Chain	Residue
A	GLU180
A	TYR573
A	GLN675
A	ASN679
A	PO4804
A	HOH921
A	HOH954
A	HOH919
A	HOH1146

---

site_id	AC6
Number of Residues	7
Details	binding site for residue GOL A 806

Chain	Residue
A	LYS265
A	LYS423
A	ALA424
A	LEU431
A	HOH932
A	HOH1055
A	HOH956

---

site_id	AC7
Number of Residues	8
Details	binding site for residue GOL A 807

Chain	Residue
A	ILE35
A	TRP36
A	TYR37
A	ASN38
A	TYR47
A	ASP76
A	ASN78
A	HOH1076

---

site_id	AC8
Number of Residues	6
Details	binding site for residue GOL A 808

Chain	Residue
A	LYS16
A	GLU89
A	ASN149
A	VAL151
A	ALA152
A	HOH1183

---

site_id	AC9
Number of Residues	4
Details	binding site for residue GOL A 809

Chain	Residue
A	LYS42
A	SER549
A	HIS550
A	LYS554

---

site_id	AD1
Number of Residues	6
Details	binding site for residue GOL A 810

Chain	Residue
A	PRO220
A	GLU223
A	ALA224
A	ASN235
A	HIS279
A	HOH970

---

site_id	AD2
Number of Residues	8
Details	binding site for residue GOL A 811

Chain	Residue
A	GLN19
A	GLY20
A	PHE25
A	ARG397
A	ILE398
A	LEU399
A	GLN594
A	HOH976

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	52
Details	Domain: {"description":"Myosin N-terminal SH3-like","evidences":[{"source":"PROSITE-ProRule","id":"PRU01190","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	22
Details	Region: {"description":"Actin-binding"}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	7
Details	Binding site: {}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	1
Details	Modified residue: {"description":"N6,N6-dimethyllysine","evidences":[{"evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

Catalytic Information from CSA

site_id	MCSA1
Number of Residues	8
Details	M-CSA 534

Chain	Residue	Details
A	SER181	proton acceptor, proton donor, proton relay
A	GLY182	electrostatic stabiliser
A	THR186	metal ligand
A	ASN233	electrostatic stabiliser
A	SER236	proton acceptor, proton donor, proton relay
A	SER237	metal ligand
A	GLY457	electrostatic stabiliser
A	ARG459	electrostatic stabiliser, proton acceptor, proton donor

---