4OMF
The F420-reducing [NiFe]-hydrogenase complex from Methanothermobacter marburgensis, the first X-ray structure of a group 3 family member
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0008901 | molecular_function | ferredoxin hydrogenase activity |
| A | 0016151 | molecular_function | nickel cation binding |
| A | 0016491 | molecular_function | oxidoreductase activity |
| A | 0046872 | molecular_function | metal ion binding |
| A | 0050454 | molecular_function | coenzyme F420 hydrogenase activity |
| A | 0050660 | molecular_function | flavin adenine dinucleotide binding |
| A | 0051536 | molecular_function | iron-sulfur cluster binding |
| B | 0000166 | molecular_function | nucleotide binding |
| B | 0016151 | molecular_function | nickel cation binding |
| B | 0016491 | molecular_function | oxidoreductase activity |
| B | 0046872 | molecular_function | metal ion binding |
| B | 0050454 | molecular_function | coenzyme F420 hydrogenase activity |
| B | 0050660 | molecular_function | flavin adenine dinucleotide binding |
| B | 0051536 | molecular_function | iron-sulfur cluster binding |
| B | 0051539 | molecular_function | 4 iron, 4 sulfur cluster binding |
| B | 0052592 | molecular_function | oxidoreductase activity, acting on CH or CH2 groups, with an iron-sulfur protein as acceptor |
| G | 0016151 | molecular_function | nickel cation binding |
| G | 0016491 | molecular_function | oxidoreductase activity |
| G | 0046872 | molecular_function | metal ion binding |
| G | 0050454 | molecular_function | coenzyme F420 hydrogenase activity |
| G | 0050660 | molecular_function | flavin adenine dinucleotide binding |
| G | 0051536 | molecular_function | iron-sulfur cluster binding |
| G | 0051539 | molecular_function | 4 iron, 4 sulfur cluster binding |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 9 |
| Details | BINDING SITE FOR RESIDUE SF4 G 301 |
| Chain | Residue |
| A | HIS150 |
| G | GLY56 |
| G | CYS57 |
| G | ASP60 |
| G | SER131 |
| G | CYS132 |
| G | PHE138 |
| G | GLY170 |
| G | CYS171 |
| site_id | AC2 |
| Number of Residues | 10 |
| Details | BINDING SITE FOR RESIDUE SF4 G 302 |
| Chain | Residue |
| G | GLY207 |
| G | CYS230 |
| G | THR232 |
| G | LEU235 |
| G | LEU244 |
| G | CYS249 |
| G | ILE250 |
| G | CYS252 |
| G | GLY253 |
| G | CYS255 |
| site_id | AC3 |
| Number of Residues | 8 |
| Details | BINDING SITE FOR RESIDUE SF4 G 303 |
| Chain | Residue |
| G | CYS220 |
| G | ILE221 |
| G | GLY222 |
| G | CYS223 |
| G | GLY224 |
| G | CYS226 |
| G | CYS259 |
| G | PRO260 |
| site_id | AC4 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE DTZ G 304 |
| Chain | Residue |
| G | CYS206 |
| G | CYS206 |
| G | CYS208 |
| G | CYS208 |
| G | LYS251 |
| G | HOH565 |
| site_id | AC5 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE MG A 501 |
| Chain | Residue |
| A | GLU233 |
| A | HOH666 |
| A | HOH707 |
| G | ALA148 |
| G | HOH475 |
| site_id | AC6 |
| Number of Residues | 12 |
| Details | BINDING SITE FOR RESIDUE NFU A 502 |
| Chain | Residue |
| A | CYS63 |
| A | CYS66 |
| A | HIS70 |
| A | ALA326 |
| A | PRO327 |
| A | ARG328 |
| A | ASP331 |
| A | VAL349 |
| A | PRO350 |
| A | THR351 |
| A | CYS380 |
| A | CYS383 |
| site_id | AC7 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE MG A 503 |
| Chain | Residue |
| A | GLU44 |
| A | ALA347 |
| A | HIS386 |
| A | HOH961 |
| A | HOH962 |
| A | HOH963 |
| site_id | AC8 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE MG A 504 |
| Chain | Residue |
| A | HOH601 |
| A | HOH925 |
| A | HOH926 |
| A | HOH959 |
| A | HOH965 |
| A | HOH966 |
| site_id | AC9 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE CL A 505 |
| Chain | Residue |
| A | ILE156 |
| A | ARG308 |
| A | HOH683 |
| A | HOH699 |
| site_id | BC1 |
| Number of Residues | 12 |
| Details | BINDING SITE FOR RESIDUE SF4 B 401 |
| Chain | Residue |
| B | ILE102 |
| B | PRO103 |
| B | CYS104 |
| B | CYS134 |
| B | MET135 |
| B | GLU136 |
| B | ASN137 |
| B | CYS192 |
| B | CYS195 |
| B | LYS261 |
| G | ILE221 |
| G | ARG261 |
| site_id | BC2 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE MG B 402 |
| Chain | Residue |
| B | HOH501 |
| B | HOH570 |
| B | HOH626 |
| B | HOH660 |
| B | HOH694 |
| B | HOH695 |
| site_id | BC3 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE KEN B 403 |
| Chain | Residue |
| B | ARG110 |
| B | GLN113 |
| B | ILE126 |
| B | ASP204 |
| site_id | BC4 |
| Number of Residues | 31 |
| Details | BINDING SITE FOR RESIDUE FAD B 405 |
| Chain | Residue |
| B | GLY73 |
| B | THR74 |
| B | LYS75 |
| B | TYR76 |
| B | THR77 |
| B | SER79 |
| B | ASN81 |
| B | TYR133 |
| B | CYS134 |
| B | MET135 |
| B | GLU136 |
| B | ASN137 |
| B | TYR198 |
| B | GLY208 |
| B | SER209 |
| B | VAL210 |
| B | HOH507 |
| B | HOH514 |
| B | HOH591 |
| B | HOH619 |
| B | ALA23 |
| B | GLN24 |
| B | ASP25 |
| B | GLY26 |
| B | GLY27 |
| B | ILE28 |
| B | VAL29 |
| B | THR30 |
| B | VAL47 |
| B | ALA48 |
| B | ALA71 |
Functional Information from PROSITE/UniProt
| site_id | PS00198 |
| Number of Residues | 12 |
| Details | 4FE4S_FER_1 4Fe-4S ferredoxin-type iron-sulfur binding region signature. CiKCGiCYvQCP |
| Chain | Residue | Details |
| G | CYS249-PRO260 |
| site_id | PS00507 |
| Number of Residues | 26 |
| Details | NI_HGENASE_L_1 Nickel-dependent hydrogenases large subunit signature 1. RGLEkmvtgkapetapvmvQRiCGVC |
| Chain | Residue | Details |
| A | ARG41-CYS66 |
| site_id | PS00508 |
| Number of Residues | 10 |
| Details | NI_HGENASE_L_2 Nickel-dependent hydrogenases large subunit signature 2. YDPCLSCat.H |
| Chain | Residue | Details |
| A | TYR377-HIS386 |
