Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

4O9C

Crystal structure of Beta-ketothiolase (PhaA) from Ralstonia eutropha H16

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003985	molecular_function	acetyl-CoA C-acetyltransferase activity
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0006635	biological_process	fatty acid beta-oxidation
A	0016740	molecular_function	transferase activity
A	0016746	molecular_function	acyltransferase activity
A	0016747	molecular_function	acyltransferase activity, transferring groups other than amino-acyl groups
A	0042619	biological_process	poly-hydroxybutyrate biosynthetic process
B	0003985	molecular_function	acetyl-CoA C-acetyltransferase activity
B	0005737	cellular_component	cytoplasm
B	0005829	cellular_component	cytosol
B	0006635	biological_process	fatty acid beta-oxidation
B	0016740	molecular_function	transferase activity
B	0016746	molecular_function	acyltransferase activity
B	0016747	molecular_function	acyltransferase activity, transferring groups other than amino-acyl groups
B	0042619	biological_process	poly-hydroxybutyrate biosynthetic process
C	0003985	molecular_function	acetyl-CoA C-acetyltransferase activity
C	0005737	cellular_component	cytoplasm
C	0005829	cellular_component	cytosol
C	0006635	biological_process	fatty acid beta-oxidation
C	0016740	molecular_function	transferase activity
C	0016746	molecular_function	acyltransferase activity
C	0016747	molecular_function	acyltransferase activity, transferring groups other than amino-acyl groups
C	0042619	biological_process	poly-hydroxybutyrate biosynthetic process
D	0003985	molecular_function	acetyl-CoA C-acetyltransferase activity
D	0005737	cellular_component	cytoplasm
D	0005829	cellular_component	cytosol
D	0006635	biological_process	fatty acid beta-oxidation
D	0016740	molecular_function	transferase activity
D	0016746	molecular_function	acyltransferase activity
D	0016747	molecular_function	acyltransferase activity, transferring groups other than amino-acyl groups
D	0042619	biological_process	poly-hydroxybutyrate biosynthetic process
E	0003985	molecular_function	acetyl-CoA C-acetyltransferase activity
E	0005737	cellular_component	cytoplasm
E	0005829	cellular_component	cytosol
E	0006635	biological_process	fatty acid beta-oxidation
E	0016740	molecular_function	transferase activity
E	0016746	molecular_function	acyltransferase activity
E	0016747	molecular_function	acyltransferase activity, transferring groups other than amino-acyl groups
E	0042619	biological_process	poly-hydroxybutyrate biosynthetic process
F	0003985	molecular_function	acetyl-CoA C-acetyltransferase activity
F	0005737	cellular_component	cytoplasm
F	0005829	cellular_component	cytosol
F	0006635	biological_process	fatty acid beta-oxidation
F	0016740	molecular_function	transferase activity
F	0016746	molecular_function	acyltransferase activity
F	0016747	molecular_function	acyltransferase activity, transferring groups other than amino-acyl groups
F	0042619	biological_process	poly-hydroxybutyrate biosynthetic process
G	0003985	molecular_function	acetyl-CoA C-acetyltransferase activity
G	0005737	cellular_component	cytoplasm
G	0005829	cellular_component	cytosol
G	0006635	biological_process	fatty acid beta-oxidation
G	0016740	molecular_function	transferase activity
G	0016746	molecular_function	acyltransferase activity
G	0016747	molecular_function	acyltransferase activity, transferring groups other than amino-acyl groups
G	0042619	biological_process	poly-hydroxybutyrate biosynthetic process
H	0003985	molecular_function	acetyl-CoA C-acetyltransferase activity
H	0005737	cellular_component	cytoplasm
H	0005829	cellular_component	cytosol
H	0006635	biological_process	fatty acid beta-oxidation
H	0016740	molecular_function	transferase activity
H	0016746	molecular_function	acyltransferase activity
H	0016747	molecular_function	acyltransferase activity, transferring groups other than amino-acyl groups
H	0042619	biological_process	poly-hydroxybutyrate biosynthetic process

Functional Information from PDB Data

site_id	AC1
Number of Residues	14
Details	BINDING SITE FOR RESIDUE COA C 401

Chain	Residue
C	SER88
C	ALA319
C	PHE320
C	HIS349
C	HOH513
H	MET134
C	LEU148
C	HIS156
C	ARG221
C	PHE236
C	ALA244
C	SER248
C	GLY249
C	LEU250

Functional Information from PROSITE/UniProt

site_id	PS00099
Number of Residues	14
Details	THIOLASE_3 Thiolases active site. GLASLCIGgGmGvA

Chain	Residue	Details
A	GLY374-ALA387

site_id	PS00737
Number of Residues	17
Details	THIOLASE_2 Thiolases signature 2. NvnGGaIAiGHPiGaSG

Chain	Residue	Details
A	ASN339-GLY355

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	8
Details	ACT_SITE: Acyl-thioester intermediate => ECO:0000269\|PubMed:25152395

Chain	Residue	Details
A	SER88
B	SER88
C	SER88
D	SER88
E	SER88
F	SER88
G	SER88
H	SER88

site_id	SWS_FT_FI2
Number of Residues	16
Details	ACT_SITE: Proton acceptor => ECO:0000255\|PROSITE-ProRule:PRU10020, ECO:0000305\|PubMed:25152395

Chain	Residue	Details
A	HIS349
E	CYS379
F	HIS349
F	CYS379
G	HIS349
G	CYS379
H	HIS349
H	CYS379
A	CYS379
B	HIS349
B	CYS379
C	HIS349
C	CYS379
D	HIS349
D	CYS379
E	HIS349

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)