Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

4NKX

Human steroidogenic cytochrome P450 17A1 mutant A105L with substrate progesterone

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004497	molecular_function	monooxygenase activity
A	0004508	molecular_function	steroid 17-alpha-monooxygenase activity
A	0005506	molecular_function	iron ion binding
A	0005783	cellular_component	endoplasmic reticulum
A	0005789	cellular_component	endoplasmic reticulum membrane
A	0006629	biological_process	lipid metabolic process
A	0006694	biological_process	steroid biosynthetic process
A	0006702	biological_process	androgen biosynthetic process
A	0006704	biological_process	glucocorticoid biosynthetic process
A	0007548	biological_process	sex differentiation
A	0008202	biological_process	steroid metabolic process
A	0008395	molecular_function	steroid hydroxylase activity
A	0016020	cellular_component	membrane
A	0016491	molecular_function	oxidoreductase activity
A	0016705	molecular_function	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
A	0016712	molecular_function	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen
A	0016829	molecular_function	lyase activity
A	0019825	molecular_function	oxygen binding
A	0020037	molecular_function	heme binding
A	0030424	cellular_component	axon
A	0034651	biological_process	cortisol biosynthetic process
A	0042445	biological_process	hormone metabolic process
A	0042446	biological_process	hormone biosynthetic process
A	0042448	biological_process	progesterone metabolic process
A	0043025	cellular_component	neuronal cell body
A	0046872	molecular_function	metal ion binding
A	0120254	biological_process	olefinic compound metabolic process
B	0004497	molecular_function	monooxygenase activity
B	0004508	molecular_function	steroid 17-alpha-monooxygenase activity
B	0005506	molecular_function	iron ion binding
B	0005783	cellular_component	endoplasmic reticulum
B	0005789	cellular_component	endoplasmic reticulum membrane
B	0006629	biological_process	lipid metabolic process
B	0006694	biological_process	steroid biosynthetic process
B	0006702	biological_process	androgen biosynthetic process
B	0006704	biological_process	glucocorticoid biosynthetic process
B	0007548	biological_process	sex differentiation
B	0008202	biological_process	steroid metabolic process
B	0008395	molecular_function	steroid hydroxylase activity
B	0016020	cellular_component	membrane
B	0016491	molecular_function	oxidoreductase activity
B	0016705	molecular_function	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
B	0016712	molecular_function	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen
B	0016829	molecular_function	lyase activity
B	0019825	molecular_function	oxygen binding
B	0020037	molecular_function	heme binding
B	0030424	cellular_component	axon
B	0034651	biological_process	cortisol biosynthetic process
B	0042445	biological_process	hormone metabolic process
B	0042446	biological_process	hormone biosynthetic process
B	0042448	biological_process	progesterone metabolic process
B	0043025	cellular_component	neuronal cell body
B	0046872	molecular_function	metal ion binding
B	0120254	biological_process	olefinic compound metabolic process
C	0004497	molecular_function	monooxygenase activity
C	0004508	molecular_function	steroid 17-alpha-monooxygenase activity
C	0005506	molecular_function	iron ion binding
C	0005783	cellular_component	endoplasmic reticulum
C	0005789	cellular_component	endoplasmic reticulum membrane
C	0006629	biological_process	lipid metabolic process
C	0006694	biological_process	steroid biosynthetic process
C	0006702	biological_process	androgen biosynthetic process
C	0006704	biological_process	glucocorticoid biosynthetic process
C	0007548	biological_process	sex differentiation
C	0008202	biological_process	steroid metabolic process
C	0008395	molecular_function	steroid hydroxylase activity
C	0016020	cellular_component	membrane
C	0016491	molecular_function	oxidoreductase activity
C	0016705	molecular_function	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
C	0016712	molecular_function	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen
C	0016829	molecular_function	lyase activity
C	0019825	molecular_function	oxygen binding
C	0020037	molecular_function	heme binding
C	0030424	cellular_component	axon
C	0034651	biological_process	cortisol biosynthetic process
C	0042445	biological_process	hormone metabolic process
C	0042446	biological_process	hormone biosynthetic process
C	0042448	biological_process	progesterone metabolic process
C	0043025	cellular_component	neuronal cell body
C	0046872	molecular_function	metal ion binding
C	0120254	biological_process	olefinic compound metabolic process
D	0004497	molecular_function	monooxygenase activity
D	0004508	molecular_function	steroid 17-alpha-monooxygenase activity
D	0005506	molecular_function	iron ion binding
D	0005783	cellular_component	endoplasmic reticulum
D	0005789	cellular_component	endoplasmic reticulum membrane
D	0006629	biological_process	lipid metabolic process
D	0006694	biological_process	steroid biosynthetic process
D	0006702	biological_process	androgen biosynthetic process
D	0006704	biological_process	glucocorticoid biosynthetic process
D	0007548	biological_process	sex differentiation
D	0008202	biological_process	steroid metabolic process
D	0008395	molecular_function	steroid hydroxylase activity
D	0016020	cellular_component	membrane
D	0016491	molecular_function	oxidoreductase activity
D	0016705	molecular_function	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
D	0016712	molecular_function	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen
D	0016829	molecular_function	lyase activity
D	0019825	molecular_function	oxygen binding
D	0020037	molecular_function	heme binding
D	0030424	cellular_component	axon
D	0034651	biological_process	cortisol biosynthetic process
D	0042445	biological_process	hormone metabolic process
D	0042446	biological_process	hormone biosynthetic process
D	0042448	biological_process	progesterone metabolic process
D	0043025	cellular_component	neuronal cell body
D	0046872	molecular_function	metal ion binding
D	0120254	biological_process	olefinic compound metabolic process

Functional Information from PDB Data

site_id	AC1
Number of Residues	18
Details	BINDING SITE FOR RESIDUE HEM A 600

Chain	Residue
A	ARG96
A	VAL366
A	ILE371
A	HIS373
A	PRO434
A	PHE435
A	ARG440
A	SER441
A	CYS442
A	GLY444
A	ILE112
A	ALA113
A	TRP121
A	ARG125
A	ILE299
A	ALA302
A	THR306
A	VAL310

site_id	AC2
Number of Residues	10
Details	BINDING SITE FOR RESIDUE STR A 601

Chain	Residue
A	ALA113
A	PHE114
A	ASN202
A	ILE205
A	ASP298
A	GLY301
A	GLU305
A	THR306
A	ILE371
A	VAL483

site_id	AC3
Number of Residues	18
Details	BINDING SITE FOR RESIDUE HEM B 600

Chain	Residue
B	ARG96
B	ILE112
B	ALA113
B	TRP121
B	ARG125
B	ALA302
B	GLY303
B	THR306
B	VAL310
B	VAL366
B	LEU370
B	ILE371
B	HIS373
B	PRO434
B	PHE435
B	ARG440
B	CYS442
B	ALA448

site_id	AC4
Number of Residues	6
Details	BINDING SITE FOR RESIDUE STR B 601

Chain	Residue
B	ALA113
B	ASN202
B	ASP298
B	THR306
B	ILE371
B	VAL483

site_id	AC5
Number of Residues	21
Details	BINDING SITE FOR RESIDUE HEM C 600

Chain	Residue
C	ARG96
C	ILE112
C	ALA113
C	TRP121
C	ARG125
C	ILE299
C	ALA302
C	GLY303
C	THR306
C	THR307
C	VAL366
C	LEU370
C	ILE371
C	HIS373
C	PRO434
C	PHE435
C	ARG440
C	CYS442
C	ILE443
C	ALA448
C	STR601

site_id	AC6
Number of Residues	8
Details	BINDING SITE FOR RESIDUE STR C 601

Chain	Residue
C	ALA113
C	ASN202
C	ILE205
C	ASP298
C	VAL366
C	ILE371
C	VAL483
C	HEM600

site_id	AC7
Number of Residues	20
Details	BINDING SITE FOR RESIDUE HEM D 600

Chain	Residue
D	ALA448
D	STR601
D	ARG96
D	ILE112
D	ALA113
D	TRP121
D	ARG125
D	ALA302
D	GLY303
D	THR306
D	VAL310
D	VAL366
D	LEU370
D	HIS373
D	PRO434
D	PHE435
D	ARG440
D	CYS442
D	ILE443
D	GLY444

site_id	AC8
Number of Residues	9
Details	BINDING SITE FOR RESIDUE STR D 601

Chain	Residue
D	ALA113
D	ASN202
D	ILE205
D	ASP298
D	THR306
D	VAL366
D	ILE371
D	VAL483
D	HEM600

Functional Information from PROSITE/UniProt

site_id	PS00086
Number of Residues	10
Details	CYTOCHROME_P450 Cytochrome P450 cysteine heme-iron ligand signature. FGaGPRSCIG

Chain	Residue	Details
A	PHE435-GLY444

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	4
Details	Binding site: {"evidences":[{"source":"PubMed","id":"25301938","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI2
Number of Residues	4
Details	Binding site: {"description":"axial binding residue"}

Chain

Residue

Details

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)