4NCY

In situ trypsin crystallized on a MiTeGen micromesh with imidazole ligand

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004175	molecular_function	endopeptidase activity
A	0004252	molecular_function	serine-type endopeptidase activity
A	0005515	molecular_function	protein binding
A	0005576	cellular_component	extracellular region
A	0005615	cellular_component	extracellular space
A	0006508	biological_process	proteolysis
A	0007586	biological_process	digestion
A	0008233	molecular_function	peptidase activity
A	0008236	molecular_function	serine-type peptidase activity
A	0016787	molecular_function	hydrolase activity
A	0046872	molecular_function	metal ion binding
A	0097180	cellular_component	serine protease inhibitor complex
A	0097655	molecular_function	serpin family protein binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	6
Details	BINDING SITE FOR RESIDUE CA A 301

Chain	Residue
A	GLU52
A	ASN54
A	VAL57
A	GLU62
A	HOH402
A	HOH410

---

site_id	AC2
Number of Residues	12
Details	BINDING SITE FOR RESIDUE BEN A 302

Chain	Residue
A	GLN174
A	SER177
A	TRP193
A	GLY194
A	GLY196
A	GLY204
A	HOH428
A	HOH808
A	HOH810
A	ASP171
A	SER172
A	CYS173

---

site_id	AC3
Number of Residues	10
Details	BINDING SITE FOR RESIDUE EDO A 303

Chain	Residue
A	ARG49
A	ILE55
A	ASN56
A	VAL58
A	VAL72
A	HIS73
A	PRO74
A	EDO304
A	HOH410
A	HOH438

---

site_id	AC4
Number of Residues	8
Details	BINDING SITE FOR RESIDUE EDO A 304

Chain	Residue
A	TYR22
A	HIS23
A	ILE55
A	ASN56
A	VAL72
A	EDO303
A	HOH449
A	HOH712

---

site_id	AC5
Number of Residues	7
Details	BINDING SITE FOR RESIDUE EDO A 305

Chain	Residue
A	CYS109
A	ALA110
A	PHE161
A	GLN188
A	LYS208
A	HOH417
A	HOH775

---

site_id	AC6
Number of Residues	1
Details	BINDING SITE FOR RESIDUE EDO A 306

Chain	Residue
A	HOH595

---

site_id	AC7
Number of Residues	5
Details	BINDING SITE FOR RESIDUE EDO A 307

Chain	Residue
A	VAL58
A	ASN77
A	ASN79
A	HOH424
A	HOH639

---

site_id	AC8
Number of Residues	10
Details	BINDING SITE FOR RESIDUE EDO A 308

Chain	Residue
A	GLY45
A	ALA67
A	SER68
A	LYS69
A	SER130
A	TYR131
A	HOH645
A	HOH726
A	HOH805
A	HOH817

---

site_id	AC9
Number of Residues	9
Details	BINDING SITE FOR RESIDUE SO4 A 309

Chain	Residue
A	HIS40
A	GLN174
A	GLY175
A	SER177
A	HOH602
A	HOH642
A	HOH644
A	HOH674
A	HOH694

---

site_id	BC1
Number of Residues	10
Details	BINDING SITE FOR RESIDUE SO4 A 310

Chain	Residue
A	PRO106
A	THR107
A	SER108
A	GLY128
A	LYS186
A	GLN188
A	IMD314
A	HOH441
A	HOH627
A	HOH766

---

site_id	BC2
Number of Residues	7
Details	BINDING SITE FOR RESIDUE SO4 A 311

Chain	Residue
A	LYS149
A	PRO153
A	GLY154
A	HOH430
A	HOH584
A	HOH604
A	HOH776

---

site_id	BC3
Number of Residues	8
Details	BINDING SITE FOR RESIDUE SO4 A 312

Chain	Residue
A	LYS69
A	LYS89
A	THR129
A	HOH525
A	HOH623
A	HOH684
A	HOH725
A	HOH766

---

site_id	BC4
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 A 313

Chain	Residue
A	LYS125
A	SER126
A	SER127
A	HOH697

---

site_id	BC5
Number of Residues	9
Details	BINDING SITE FOR RESIDUE IMD A 314

Chain	Residue
A	SER126
A	GLY128
A	GLN174
A	SER184
A	LYS186
A	CYS197
A	SO4310
A	ASN123
A	LYS125

---

Functional Information from PROSITE/UniProt

site_id	PS00134
Number of Residues	6
Details	TRYPSIN_HIS Serine proteases, trypsin family, histidine active site. VSAAHC

Chain	Residue	Details
A	VAL36-CYS41

---

site_id	PS00135
Number of Residues	12
Details	TRYPSIN_SER Serine proteases, trypsin family, serine active site. DScqGDSGGPVV

Chain	Residue	Details
A	ASP171-VAL182

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	220
Details	Domain: {"description":"Peptidase S1","evidences":[{"source":"PROSITE-ProRule","id":"PRU00274","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	3
Details	Active site: {"description":"Charge relay system"}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	7
Details	Binding site: {}

Chain

Residue

Details

---