4N66

Thermolysin in complex with UBTLN37

Functional Information from GO Data

Chain	GOid	namespace	contents
E	0004222	molecular_function	metalloendopeptidase activity
E	0006508	biological_process	proteolysis

Functional Information from PDB Data

site_id	AC1
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN E 501

Chain	Residue
E	HIS142
E	HIS146
E	GLU166
E	2GZ513

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site_id	AC2
Number of Residues	6
Details	BINDING SITE FOR RESIDUE CA E 502

Chain	Residue
E	HOH601
E	ASP138
E	GLU177
E	ASP185
E	GLU187
E	GLU190

---

site_id	AC3
Number of Residues	6
Details	BINDING SITE FOR RESIDUE CA E 503

Chain	Residue
E	ASP57
E	ASP59
E	GLN61
E	HOH619
E	HOH620
E	HOH627

---

site_id	AC4
Number of Residues	6
Details	BINDING SITE FOR RESIDUE CA E 504

Chain	Residue
E	TYR193
E	THR194
E	ILE197
E	ASP200
E	HOH628
E	HOH684

---

site_id	AC5
Number of Residues	6
Details	BINDING SITE FOR RESIDUE CA E 505

Chain	Residue
E	GLU177
E	ASN183
E	ASP185
E	GLU190
E	HOH645
E	HOH658

---

site_id	AC6
Number of Residues	5
Details	BINDING SITE FOR RESIDUE DMS E 506

Chain	Residue
E	ILE1
E	THR2
E	GLY3
E	GLN31
E	ASN33

---

site_id	AC7
Number of Residues	5
Details	BINDING SITE FOR RESIDUE DMS E 507

Chain	Residue
E	TYR66
E	HIS216
E	SER218
E	TYR251
E	HOH663

---

site_id	AC8
Number of Residues	5
Details	BINDING SITE FOR RESIDUE DMS E 508

Chain	Residue
E	TYR110
E	ASN112
E	PHE114
E	2GZ513
E	2GZ513

---

site_id	AC9
Number of Residues	5
Details	BINDING SITE FOR RESIDUE DMS E 509

Chain	Residue
E	GLY95
E	PRO184
E	TRP186
E	HOH655
E	HOH783

---

site_id	BC1
Number of Residues	8
Details	BINDING SITE FOR RESIDUE GOL E 510

Chain	Residue
E	PHE114
E	TRP115
E	HIS146
E	TYR157
E	2GZ513
E	HOH609
E	HOH646
E	HOH648

---

site_id	BC2
Number of Residues	6
Details	BINDING SITE FOR RESIDUE GOL E 511

Chain	Residue
E	GLY109
E	TYR110
E	ASN111
E	ASN112
E	GLN158
E	HOH894

---

site_id	BC3
Number of Residues	11
Details	BINDING SITE FOR RESIDUE GOL E 512

Chain	Residue
E	GLY247
E	GLY248
E	VAL255
E	GLN273
E	TYR274
E	LEU275
E	HOH632
E	HOH664
E	HOH672
E	HOH687
E	HOH849

---

site_id	BC4
Number of Residues	20
Details	BINDING SITE FOR RESIDUE 2GZ E 513

Chain	Residue
E	TYR106
E	ASN112
E	ALA113
E	PHE114
E	TRP115
E	VAL139
E	HIS142
E	GLU143
E	HIS146
E	TYR157
E	GLU166
E	LEU202
E	ARG203
E	HIS231
E	ZN501
E	DMS508
E	DMS508
E	GOL510
E	HOH673
E	HOH947

---

Functional Information from PROSITE/UniProt

site_id	PS00142
Number of Residues	10
Details	ZINC_PROTEASE Neutral zinc metallopeptidases, zinc-binding region signature. VVAHELTHAV

Chain	Residue	Details
E	VAL139-VAL148

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1
Details	ACT_SITE: ACT_SITE => ECO:0000255|PROSITE-ProRule:PRU10095, ECO:0000269|PubMed:4808703

Chain	Residue	Details
E	GLU143

---

site_id	SWS_FT_FI2
Number of Residues	1
Details	ACT_SITE: Proton donor => ECO:0000255|PROSITE-ProRule:PRU10095, ECO:0000269|PubMed:4808703

Chain	Residue	Details
E	HIS231

---

site_id	SWS_FT_FI3
Number of Residues	16
Details	BINDING:

Chain	Residue	Details
E	ASP57
E	ASP185
E	GLU187
E	GLU190
E	TYR193
E	THR194
E	ILE197
E	ASP200
E	ASP59
E	GLN61
E	ASP138
E	HIS142
E	HIS146
E	GLU166
E	GLU177
E	ASN183

---

Catalytic Information from CSA

site_id	MCSA1
Number of Residues	7
Details	M-CSA 176

Chain	Residue	Details
E	HIS142	metal ligand
E	GLU143	electrostatic stabiliser, metal ligand
E	HIS146	metal ligand
E	TYR157	electrostatic stabiliser, hydrogen bond donor, steric role
E	GLU166	metal ligand
E	ASP226	activator, electrostatic stabiliser, hydrogen bond acceptor
E	HIS231	hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor

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