Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

4N4N

Nitrosomonas europea HAO

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0016491	molecular_function	oxidoreductase activity
A	0019331	biological_process	anaerobic respiration, using ammonium as electron donor
A	0020037	molecular_function	heme binding
A	0033740	molecular_function	hydroxylamine oxidoreductase activity
A	0042802	molecular_function	identical protein binding
A	0044222	cellular_component	anammoxosome
A	0046872	molecular_function	metal ion binding
A	0047991	molecular_function	hydroxylamine oxidase activity
A	0070207	biological_process	protein homotrimerization
A	0140305	molecular_function	hydroxylamine dehydrogenase activity
C	0016491	molecular_function	oxidoreductase activity
C	0019331	biological_process	anaerobic respiration, using ammonium as electron donor
C	0020037	molecular_function	heme binding
C	0033740	molecular_function	hydroxylamine oxidoreductase activity
C	0042802	molecular_function	identical protein binding
C	0044222	cellular_component	anammoxosome
C	0046872	molecular_function	metal ion binding
C	0047991	molecular_function	hydroxylamine oxidase activity
C	0070207	biological_process	protein homotrimerization
C	0140305	molecular_function	hydroxylamine dehydrogenase activity
E	0016491	molecular_function	oxidoreductase activity
E	0019331	biological_process	anaerobic respiration, using ammonium as electron donor
E	0020037	molecular_function	heme binding
E	0033740	molecular_function	hydroxylamine oxidoreductase activity
E	0042802	molecular_function	identical protein binding
E	0044222	cellular_component	anammoxosome
E	0046872	molecular_function	metal ion binding
E	0047991	molecular_function	hydroxylamine oxidase activity
E	0070207	biological_process	protein homotrimerization
E	0140305	molecular_function	hydroxylamine dehydrogenase activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	21
Details	BINDING SITE FOR RESIDUE HEC A 601

Chain	Residue
A	TYR140
A	HIS267
A	SER277
A	ARG278
A	ARG319
A	LEU320
A	MET339
A	HIS347
A	HEC602
A	HEC606
A	HOH767
A	LYS141
A	HOH785
A	HOH979
A	CYS196
A	HIS200
A	GLU203
A	HIS228
A	ASN259
A	CYS263
A	CYS266

site_id	AC2
Number of Residues	19
Details	BINDING SITE FOR RESIDUE HEC A 602

Chain	Residue
A	THR122
A	HIS123
A	LYS141
A	LYS144
A	LEU145
A	VAL148
A	VAL167
A	CYS196
A	CYS199
A	HIS200
A	CYS263
A	HIS267
A	PHE272
A	SER273
A	ALA274
A	HEC601
A	HEC604
A	HOH825
A	HOH903

site_id	AC3
Number of Residues	17
Details	BINDING SITE FOR RESIDUE HEC A 603

Chain	Residue
A	TYR81
A	TYR88
A	ASP102
A	CYS103
A	CYS106
A	HIS107
A	GLU110
A	CYS169
A	HIS173
A	HIS184
A	ILE188
A	MET190
A	HEC604
A	HOH838
C	SER389
C	GLU390
C	ARG391

site_id	AC4
Number of Residues	23
Details	BINDING SITE FOR RESIDUE HEC A 604

Chain	Residue
A	TYR81
A	PRO84
A	HIS107
A	TRP114
A	TRP118
A	HIS123
A	VAL167
A	GLY168
A	CYS169
A	CYS172
A	HIS173
A	MET190
A	PRO191
A	LYS262
A	ASP264
A	ARG269
A	HIS270
A	PHE272
A	HEC602
A	HEC603
C	HIS388
C	SER389
C	HOH776

site_id	AC5
Number of Residues	20
Details	BINDING SITE FOR RESIDUE HEC A 605

Chain	Residue
C	HEC601
A	HIS287
A	ASN294
A	TYR298
A	PRO332
A	THR333
A	CYS334
A	CYS337
A	HIS338
A	THR353
A	ARG354
A	TRP355
A	ALA356
A	TRP380
A	MET400
A	HIS483
A	HEC606
A	HEC607
A	HOH711
A	HOH858

site_id	AC6
Number of Residues	23
Details	BINDING SITE FOR RESIDUE HEC A 606

Chain	Residue
A	PRO226
A	SER227
A	HIS228
A	ASP231
A	ALA234
A	CYS256
A	HIS257
A	ASN259
A	ASN265
A	CYS266
A	ALA282
A	CYS283
A	CYS286
A	HIS287
A	CYS334
A	HIS338
A	ILE349
A	THR353
A	ALA356
A	HEC601
A	HEC605
A	HOH749
C	HEC601

site_id	AC7
Number of Residues	22
Details	BINDING SITE FOR RESIDUE HEC A 607

Chain	Residue
A	HIS303
A	LEU306
A	PHE324
A	ASN330
A	ALA331
A	PRO332
A	THR383
A	CYS384
A	CYS387
A	HIS388
A	PHE392
A	TYR396
A	VAL484
A	HEC605
A	HOH709
A	HOH908
E	LYS262
E	ASP264
E	THR268
E	ARG269
E	HOH719
E	HOH818

site_id	AC8
Number of Residues	23
Details	BINDING SITE FOR RESIDUE HEC A 608

Chain	Residue
A	THR490
A	TYR491
A	HOH788
A	HOH971
E	TRP221
E	ARG225
E	ALA234
E	ASN235
E	THR238
E	TRP241
E	CYS253
E	CYS256
E	HIS257
E	THR285
E	CYS286
E	HIS287
E	HIS292
E	ASN357
E	PHE452
E	HEC606
E	HEC607
E	HOH713
E	HOH779

site_id	AC9
Number of Residues	4
Details	BINDING SITE FOR RESIDUE K A 609

Chain	Residue
A	ASP312
A	HOH916
C	ASP312
E	ASP312

site_id	BC1
Number of Residues	24
Details	BINDING SITE FOR RESIDUE HEC C 601

Chain	Residue
A	TRP221
A	ARG225
A	ALA234
A	ASN235
A	THR238
A	TRP241
A	GLY252
A	CYS253
A	CYS256
A	HIS257
A	THR285
A	CYS286
A	HIS287
A	HIS292
A	ASN357
A	PHE452
A	HEC605
A	HEC606
A	HOH775
A	HOH968
C	THR490
C	TYR491
C	HOH704
C	HOH851

site_id	BC2
Number of Residues	21
Details	BINDING SITE FOR RESIDUE HEC C 602

Chain	Residue
C	TYR140
C	LYS141
C	CYS196
C	HIS200
C	GLU203
C	HIS228
C	ASN259
C	CYS263
C	CYS266
C	HIS267
C	SER277
C	ARG278
C	ARG319
C	LEU320
C	MET339
C	HIS347
C	HEC603
C	HEC607
C	HOH710
C	HOH770
C	HOH873

site_id	BC3
Number of Residues	19
Details	BINDING SITE FOR RESIDUE HEC C 603

Chain	Residue
C	THR122
C	HIS123
C	LYS141
C	LYS144
C	LEU145
C	VAL167
C	VAL176
C	CYS196
C	CYS199
C	HIS200
C	CYS263
C	HIS267
C	PHE272
C	SER273
C	ALA274
C	HEC602
C	HEC605
C	HOH798
C	HOH824

site_id	BC4
Number of Residues	20
Details	BINDING SITE FOR RESIDUE HEC C 604

Chain	Residue
A	LYS161
C	TYR81
C	TYR88
C	PRO94
C	ASP102
C	CYS103
C	CYS106
C	HIS107
C	GLU110
C	CYS169
C	HIS173
C	HIS184
C	ILE188
C	MET190
C	HEC605
C	HOH778
C	HOH817
E	SER389
E	GLU390
E	ARG391

site_id	BC5
Number of Residues	22
Details	BINDING SITE FOR RESIDUE HEC C 605

Chain	Residue
C	TYR81
C	PRO84
C	HIS107
C	TRP114
C	HIS123
C	VAL167
C	GLY168
C	CYS169
C	CYS172
C	HIS173
C	MET190
C	PRO191
C	LYS262
C	ASP264
C	ARG269
C	HIS270
C	PHE272
C	HEC603
C	HEC604
E	HIS388
E	SER389
E	HOH757

site_id	BC6
Number of Residues	21
Details	BINDING SITE FOR RESIDUE HEC C 606

Chain	Residue
C	HIS287
C	ASN294
C	TYR298
C	PRO332
C	THR333
C	CYS334
C	CYS337
C	HIS338
C	THR353
C	ARG354
C	TRP355
C	ALA356
C	TRP380
C	MET400
C	ALA482
C	HIS483
C	HEC607
C	HEC608
C	HOH702
C	HOH840
E	HEC601

site_id	BC7
Number of Residues	22
Details	BINDING SITE FOR RESIDUE HEC C 607

Chain	Residue
C	PRO226
C	SER227
C	HIS228
C	ASP231
C	ALA234
C	CYS256
C	HIS257
C	ASN259
C	ASN265
C	CYS266
C	ALA282
C	CYS283
C	CYS286
C	HIS287
C	CYS334
C	ILE349
C	THR353
C	ALA356
C	HEC602
C	HEC606
C	HOH857
E	HEC601

site_id	BC8
Number of Residues	19
Details	BINDING SITE FOR RESIDUE HEC C 608

Chain	Residue
A	LYS262
A	ASP264
A	THR268
A	ARG269
A	HOH808
C	HIS303
C	PHE324
C	ASN330
C	PRO332
C	THR383
C	CYS384
C	CYS387
C	HIS388
C	PHE392
C	TYR396
C	VAL484
C	HEC606
C	HOH776
C	HOH812

site_id	BC9
Number of Residues	23
Details	BINDING SITE FOR RESIDUE HEC E 601

Chain	Residue
C	TRP221
C	ARG225
C	ALA234
C	ASN235
C	THR238
C	GLY252
C	CYS253
C	CYS256
C	HIS257
C	THR285
C	CYS286
C	HIS287
C	HIS292
C	ASN357
C	PHE452
C	HEC606
C	HEC607
C	HOH729
E	THR490
E	TYR491
E	HOH704
E	HOH753
E	HOH948

site_id	CC1
Number of Residues	21
Details	BINDING SITE FOR RESIDUE HEC E 602

Chain	Residue
E	TYR140
E	LYS141
E	CYS196
E	HIS200
E	GLU203
E	HIS228
E	ASN259
E	CYS263
E	CYS266
E	HIS267
E	SER277
E	ARG278
E	ARG319
E	LEU320
E	MET339
E	HIS347
E	HEC603
E	HEC607
E	HOH799
E	HOH851
E	HOH886

site_id	CC2
Number of Residues	17
Details	BINDING SITE FOR RESIDUE HEC E 603

Chain	Residue
E	THR122
E	HIS123
E	LYS141
E	LYS144
E	LEU145
E	VAL167
E	CYS196
E	CYS199
E	HIS200
E	CYS263
E	HIS267
E	PHE272
E	ALA274
E	HEC602
E	HEC605
E	HOH765
E	HOH891

site_id	CC3
Number of Residues	18
Details	BINDING SITE FOR RESIDUE HEC E 604

Chain	Residue
A	SER389
A	GLU390
A	ARG391
E	TYR81
E	TYR88
E	PRO94
E	GLU96
E	ASP102
E	CYS103
E	CYS106
E	HIS107
E	GLU110
E	HIS173
E	HIS184
E	ILE188
E	HEC605
E	HOH792
E	HOH993

site_id	CC4
Number of Residues	22
Details	BINDING SITE FOR RESIDUE HEC E 605

Chain	Residue
A	HIS388
A	SER389
A	HOH709
E	TYR81
E	PRO84
E	HIS107
E	TRP114
E	HIS123
E	VAL167
E	GLY168
E	CYS169
E	CYS172
E	HIS173
E	MET190
E	PRO191
E	LYS262
E	ASP264
E	ARG269
E	HIS270
E	PHE272
E	HEC603
E	HEC604

site_id	CC5
Number of Residues	21
Details	BINDING SITE FOR RESIDUE HEC E 606

Chain	Residue
A	HEC608
E	CYS283
E	HIS287
E	ASN294
E	TYR298
E	PRO332
E	THR333
E	CYS334
E	CYS337
E	HIS338
E	THR353
E	ARG354
E	TRP355
E	ALA356
E	TRP380
E	MET400
E	ALA482
E	HIS483
E	HEC607
E	HEC608
E	HOH706

site_id	CC6
Number of Residues	23
Details	BINDING SITE FOR RESIDUE HEC E 607

Chain	Residue
A	HEC608
E	PRO226
E	SER227
E	HIS228
E	ASP231
E	ALA234
E	CYS256
E	HIS257
E	ASN259
E	ASN265
E	CYS266
E	ALA282
E	CYS283
E	CYS286
E	HIS287
E	CYS334
E	HIS338
E	ILE349
E	THR353
E	ALA356
E	HEC602
E	HEC606
E	HOH717

site_id	CC7
Number of Residues	21
Details	BINDING SITE FOR RESIDUE HEC E 608

Chain	Residue
C	TRP114
C	LYS262
C	ASP264
C	THR268
C	ARG269
C	HOH735
E	HIS303
E	LEU306
E	ASN330
E	PRO332
E	THR383
E	CYS384
E	CYS387
E	HIS388
E	PHE392
E	TYR396
E	VAL484
E	HEC606
E	HOH757
E	HOH776
E	HOH782

site_id	CC8
Number of Residues	5
Details	BINDING SITE FOR RESIDUE PO4 E 609

Chain	Residue
E	LEU39
E	ASP40
E	LYS43
E	GLU48
E	HOH989

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	48
Details	Binding site: {"description":"covalent","evidences":[{"source":"PubMed","id":"24302732","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"9095195","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1FGJ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4N4N","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4N4O","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI2
Number of Residues	45
Details	Binding site: {"description":"axial binding residue","evidences":[{"source":"PubMed","id":"24302732","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"9095195","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1FGJ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4N4N","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4N4O","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI3
Number of Residues	3
Details	Binding site: {"evidences":[{"source":"PubMed","id":"24302732","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"4N4O","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI4
Number of Residues	3
Details	Binding site: {"description":"covalent; ligand shared between tetrameric partners","evidences":[{"source":"PubMed","id":"24302732","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

Catalytic Information from CSA

site_id	MCSA1
Number of Residues	5
Details	M-CSA 581

Chain	Residue	Details
A	HIS257	electrostatic stabiliser
A	ASP291	steric role
A	HIS292	steric role
A	TYR358	steric role
A	TYR491	activator, covalently attached

site_id	MCSA2
Number of Residues	5
Details	M-CSA 581

Chain	Residue	Details
C	HIS257	electrostatic stabiliser
C	ASP291	steric role
C	HIS292	steric role
C	TYR358	steric role
C	TYR491	activator, covalently attached

site_id	MCSA3
Number of Residues	5
Details	M-CSA 581

Chain	Residue	Details
E	HIS257	electrostatic stabiliser
E	ASP291	steric role
E	HIS292	steric role
E	TYR358	steric role
E	TYR491	activator, covalently attached

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)