4MJ8

Crystal structure of spermidine N-acetyltransferase from Vibrio cholerae in complex with polyamine

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000287	molecular_function	magnesium ion binding
A	0004145	molecular_function	diamine N-acetyltransferase activity
A	0005737	cellular_component	cytoplasm
A	0006598	biological_process	polyamine catabolic process
A	0016740	molecular_function	transferase activity
A	0016746	molecular_function	acyltransferase activity
A	0016747	molecular_function	acyltransferase activity, transferring groups other than amino-acyl groups
A	0046203	biological_process	spermidine catabolic process
A	0046208	biological_process	spermine catabolic process
A	0046872	molecular_function	metal ion binding
B	0000287	molecular_function	magnesium ion binding
B	0004145	molecular_function	diamine N-acetyltransferase activity
B	0005737	cellular_component	cytoplasm
B	0006598	biological_process	polyamine catabolic process
B	0016740	molecular_function	transferase activity
B	0016746	molecular_function	acyltransferase activity
B	0016747	molecular_function	acyltransferase activity, transferring groups other than amino-acyl groups
B	0046203	biological_process	spermidine catabolic process
B	0046208	biological_process	spermine catabolic process
B	0046872	molecular_function	metal ion binding
C	0000287	molecular_function	magnesium ion binding
C	0004145	molecular_function	diamine N-acetyltransferase activity
C	0005737	cellular_component	cytoplasm
C	0006598	biological_process	polyamine catabolic process
C	0016740	molecular_function	transferase activity
C	0016746	molecular_function	acyltransferase activity
C	0016747	molecular_function	acyltransferase activity, transferring groups other than amino-acyl groups
C	0046203	biological_process	spermidine catabolic process
C	0046208	biological_process	spermine catabolic process
C	0046872	molecular_function	metal ion binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	15
Details	BINDING SITE FOR RESIDUE SPM A 201

Chain	Residue
A	ASN22
A	HOH329
B	HIS49
B	ILE50
B	ASP52
B	GLU55
B	HOH361
A	MET28
A	GLU33
A	GLU34
A	TYR36
A	GLU37
A	GLU41
A	HOH302
A	HOH326

---

site_id	AC2
Number of Residues	10
Details	BINDING SITE FOR RESIDUE SPM A 202

Chain	Residue
A	TYR30
A	TRP31
A	GLU33
A	GLU84
A	PHE85
A	GLN86
A	ILE87
A	LEU121
A	HIS122
A	HOH381

---

site_id	AC3
Number of Residues	2
Details	BINDING SITE FOR RESIDUE EOH A 203

Chain	Residue
A	HOH308
C	SER172

---

site_id	AC4
Number of Residues	1
Details	BINDING SITE FOR RESIDUE EOH A 204

Chain	Residue
A	LYS48

---

site_id	AC5
Number of Residues	14
Details	BINDING SITE FOR RESIDUE SPM B 201

Chain	Residue
B	ASN22
B	MET28
B	GLU33
B	GLU34
B	TYR36
B	GLU41
B	HOH301
B	HOH351
B	HOH404
C	HIS49
C	ILE50
C	ASP52
C	GLU55
C	HOH302

---

site_id	AC6
Number of Residues	1
Details	BINDING SITE FOR RESIDUE EOH B 202

Chain	Residue
B	LYS48

---

site_id	AC7
Number of Residues	15
Details	BINDING SITE FOR RESIDUE SPM C 201

Chain	Residue
A	HIS49
A	ILE50
A	ASP52
A	GLU55
A	HOH341
A	HOH347
C	ASN22
C	MET28
C	GLU33
C	GLU34
C	TYR36
C	GLU37
C	GLU41
C	HOH303
C	HOH394

---

site_id	AC8
Number of Residues	2
Details	BINDING SITE FOR RESIDUE EOH C 202

Chain	Residue
C	TRP31
C	GLN86

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	471
Details	Domain: {"description":"N-acetyltransferase","evidences":[{"source":"PROSITE-ProRule","id":"PRU00532","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	3
Details	Active site: {"description":"Proton donor","evidences":[{"source":"UniProtKB","id":"P0A951","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	9
Details	Binding site: {"evidences":[{"source":"PubMed","id":"25623305","evidenceCode":"ECO:0000269"},{"source":"Reference","evidenceCode":"ECO:0000269","citation":{"citationType":"submission","publicationDate":"SEP-2013","submissionDatabase":"PDB data bank","title":"Crystal structure of spermidine N-acetyltransferase from Vibrio cholerae in complex with polyamine.","authors":["Filippova E.V.","Minasov G.","Shuvalova L.","Kiryukhina O.","Kuhn M.L.","Anderson W.F."]}},{"source":"PDB","id":"4MJ8","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	3
Details	Binding site: {"evidences":[{"source":"PubMed","id":"25623305","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"4MI4","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	3
Details	Binding site: {"evidences":[{"source":"PubMed","id":"25623305","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"4MI4","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4R87","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	9
Details	Binding site: {"evidences":[{"source":"PubMed","id":"25623305","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"4R87","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	3
Details	Binding site: {"evidences":[{"source":"PubMed","id":"25623305","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"26410587","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"5CNP","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI8
Number of Residues	24
Details	Binding site: {"evidences":[{"source":"PubMed","id":"25623305","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"4R57","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4R87","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI9
Number of Residues	27
Details	Binding site: {"evidences":[{"source":"PubMed","id":"25623305","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"4R57","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI10
Number of Residues	3
Details	Site: {"description":"Could be important for selectivity toward long polyamines","evidences":[{"source":"PubMed","id":"25623305","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---