4MD7

Crystal Structure of full-length symmetric CK2 holoenzyme

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000785	cellular_component	chromatin
A	0003682	molecular_function	chromatin binding
A	0004674	molecular_function	protein serine/threonine kinase activity
A	0005102	molecular_function	signaling receptor binding
A	0005515	molecular_function	protein binding
A	0005576	cellular_component	extracellular region
A	0005634	cellular_component	nucleus
A	0005654	cellular_component	nucleoplasm
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0005886	cellular_component	plasma membrane
A	0005956	cellular_component	protein kinase CK2 complex
A	0007165	biological_process	signal transduction
A	0008285	biological_process	negative regulation of cell population proliferation
A	0016055	biological_process	Wnt signaling pathway
A	0016605	cellular_component	PML body
A	0019887	molecular_function	protein kinase regulator activity
A	0019904	molecular_function	protein domain specific binding
A	0030674	molecular_function	protein-macromolecule adaptor activity
A	0031507	biological_process	heterochromatin formation
A	0031519	cellular_component	PcG protein complex
A	0032435	biological_process	negative regulation of proteasomal ubiquitin-dependent protein catabolic process
A	0032927	biological_process	positive regulation of activin receptor signaling pathway
A	0033211	biological_process	adiponectin-activated signaling pathway
A	0034774	cellular_component	secretory granule lumen
A	0035102	cellular_component	PRC1 complex
A	0042802	molecular_function	identical protein binding
A	0043537	biological_process	negative regulation of blood vessel endothelial cell migration
A	0045893	biological_process	positive regulation of DNA-templated transcription
A	0046872	molecular_function	metal ion binding
A	0060391	biological_process	positive regulation of SMAD protein signal transduction
A	0061154	biological_process	endothelial tube morphogenesis
A	0061629	molecular_function	RNA polymerase II-specific DNA-binding transcription factor binding
A	0065003	biological_process	protein-containing complex assembly
A	0070062	cellular_component	extracellular exosome
A	0075342	biological_process	symbiont-mediated disruption of host cell PML body
A	1903901	biological_process	negative regulation of viral life cycle
A	1904813	cellular_component	ficolin-1-rich granule lumen
B	0000785	cellular_component	chromatin
B	0003682	molecular_function	chromatin binding
B	0004674	molecular_function	protein serine/threonine kinase activity
B	0005102	molecular_function	signaling receptor binding
B	0005515	molecular_function	protein binding
B	0005576	cellular_component	extracellular region
B	0005634	cellular_component	nucleus
B	0005654	cellular_component	nucleoplasm
B	0005737	cellular_component	cytoplasm
B	0005829	cellular_component	cytosol
B	0005886	cellular_component	plasma membrane
B	0005956	cellular_component	protein kinase CK2 complex
B	0007165	biological_process	signal transduction
B	0008285	biological_process	negative regulation of cell population proliferation
B	0016055	biological_process	Wnt signaling pathway
B	0016605	cellular_component	PML body
B	0019887	molecular_function	protein kinase regulator activity
B	0019904	molecular_function	protein domain specific binding
B	0030674	molecular_function	protein-macromolecule adaptor activity
B	0031507	biological_process	heterochromatin formation
B	0031519	cellular_component	PcG protein complex
B	0032435	biological_process	negative regulation of proteasomal ubiquitin-dependent protein catabolic process
B	0032927	biological_process	positive regulation of activin receptor signaling pathway
B	0033211	biological_process	adiponectin-activated signaling pathway
B	0034774	cellular_component	secretory granule lumen
B	0035102	cellular_component	PRC1 complex
B	0042802	molecular_function	identical protein binding
B	0043537	biological_process	negative regulation of blood vessel endothelial cell migration
B	0045893	biological_process	positive regulation of DNA-templated transcription
B	0046872	molecular_function	metal ion binding
B	0060391	biological_process	positive regulation of SMAD protein signal transduction
B	0061154	biological_process	endothelial tube morphogenesis
B	0061629	molecular_function	RNA polymerase II-specific DNA-binding transcription factor binding
B	0065003	biological_process	protein-containing complex assembly
B	0070062	cellular_component	extracellular exosome
B	0075342	biological_process	symbiont-mediated disruption of host cell PML body
B	1903901	biological_process	negative regulation of viral life cycle
B	1904813	cellular_component	ficolin-1-rich granule lumen
C	0000785	cellular_component	chromatin
C	0003682	molecular_function	chromatin binding
C	0004674	molecular_function	protein serine/threonine kinase activity
C	0005102	molecular_function	signaling receptor binding
C	0005515	molecular_function	protein binding
C	0005576	cellular_component	extracellular region
C	0005634	cellular_component	nucleus
C	0005654	cellular_component	nucleoplasm
C	0005737	cellular_component	cytoplasm
C	0005829	cellular_component	cytosol
C	0005886	cellular_component	plasma membrane
C	0005956	cellular_component	protein kinase CK2 complex
C	0007165	biological_process	signal transduction
C	0008285	biological_process	negative regulation of cell population proliferation
C	0016055	biological_process	Wnt signaling pathway
C	0016605	cellular_component	PML body
C	0019887	molecular_function	protein kinase regulator activity
C	0019904	molecular_function	protein domain specific binding
C	0030674	molecular_function	protein-macromolecule adaptor activity
C	0031507	biological_process	heterochromatin formation
C	0031519	cellular_component	PcG protein complex
C	0032435	biological_process	negative regulation of proteasomal ubiquitin-dependent protein catabolic process
C	0032927	biological_process	positive regulation of activin receptor signaling pathway
C	0033211	biological_process	adiponectin-activated signaling pathway
C	0034774	cellular_component	secretory granule lumen
C	0035102	cellular_component	PRC1 complex
C	0042802	molecular_function	identical protein binding
C	0043537	biological_process	negative regulation of blood vessel endothelial cell migration
C	0045893	biological_process	positive regulation of DNA-templated transcription
C	0046872	molecular_function	metal ion binding
C	0060391	biological_process	positive regulation of SMAD protein signal transduction
C	0061154	biological_process	endothelial tube morphogenesis
C	0061629	molecular_function	RNA polymerase II-specific DNA-binding transcription factor binding
C	0065003	biological_process	protein-containing complex assembly
C	0070062	cellular_component	extracellular exosome
C	0075342	biological_process	symbiont-mediated disruption of host cell PML body
C	1903901	biological_process	negative regulation of viral life cycle
C	1904813	cellular_component	ficolin-1-rich granule lumen
D	0000785	cellular_component	chromatin
D	0003682	molecular_function	chromatin binding
D	0004674	molecular_function	protein serine/threonine kinase activity
D	0005102	molecular_function	signaling receptor binding
D	0005515	molecular_function	protein binding
D	0005576	cellular_component	extracellular region
D	0005634	cellular_component	nucleus
D	0005654	cellular_component	nucleoplasm
D	0005737	cellular_component	cytoplasm
D	0005829	cellular_component	cytosol
D	0005886	cellular_component	plasma membrane
D	0005956	cellular_component	protein kinase CK2 complex
D	0007165	biological_process	signal transduction
D	0008285	biological_process	negative regulation of cell population proliferation
D	0016055	biological_process	Wnt signaling pathway
D	0016605	cellular_component	PML body
D	0019887	molecular_function	protein kinase regulator activity
D	0019904	molecular_function	protein domain specific binding
D	0030674	molecular_function	protein-macromolecule adaptor activity
D	0031507	biological_process	heterochromatin formation
D	0031519	cellular_component	PcG protein complex
D	0032435	biological_process	negative regulation of proteasomal ubiquitin-dependent protein catabolic process
D	0032927	biological_process	positive regulation of activin receptor signaling pathway
D	0033211	biological_process	adiponectin-activated signaling pathway
D	0034774	cellular_component	secretory granule lumen
D	0035102	cellular_component	PRC1 complex
D	0042802	molecular_function	identical protein binding
D	0043537	biological_process	negative regulation of blood vessel endothelial cell migration
D	0045893	biological_process	positive regulation of DNA-templated transcription
D	0046872	molecular_function	metal ion binding
D	0060391	biological_process	positive regulation of SMAD protein signal transduction
D	0061154	biological_process	endothelial tube morphogenesis
D	0061629	molecular_function	RNA polymerase II-specific DNA-binding transcription factor binding
D	0065003	biological_process	protein-containing complex assembly
D	0070062	cellular_component	extracellular exosome
D	0075342	biological_process	symbiont-mediated disruption of host cell PML body
D	1903901	biological_process	negative regulation of viral life cycle
D	1904813	cellular_component	ficolin-1-rich granule lumen
E	0000166	molecular_function	nucleotide binding
E	0000785	cellular_component	chromatin
E	0004672	molecular_function	protein kinase activity
E	0004674	molecular_function	protein serine/threonine kinase activity
E	0005515	molecular_function	protein binding
E	0005524	molecular_function	ATP binding
E	0005634	cellular_component	nucleus
E	0005654	cellular_component	nucleoplasm
E	0005829	cellular_component	cytosol
E	0005886	cellular_component	plasma membrane
E	0005956	cellular_component	protein kinase CK2 complex
E	0006302	biological_process	double-strand break repair
E	0006457	biological_process	protein folding
E	0006468	biological_process	protein phosphorylation
E	0006915	biological_process	apoptotic process
E	0006974	biological_process	DNA damage response
E	0007165	biological_process	signal transduction
E	0008284	biological_process	positive regulation of cell population proliferation
E	0016055	biological_process	Wnt signaling pathway
E	0016301	molecular_function	kinase activity
E	0016605	cellular_component	PML body
E	0016740	molecular_function	transferase activity
E	0017148	biological_process	negative regulation of translation
E	0018105	biological_process	peptidyl-serine phosphorylation
E	0030177	biological_process	positive regulation of Wnt signaling pathway
E	0030307	biological_process	positive regulation of cell growth
E	0031507	biological_process	heterochromatin formation
E	0031519	cellular_component	PcG protein complex
E	0032435	biological_process	negative regulation of proteasomal ubiquitin-dependent protein catabolic process
E	0035102	cellular_component	PRC1 complex
E	0042176	biological_process	regulation of protein catabolic process
E	0042802	molecular_function	identical protein binding
E	0045732	biological_process	positive regulation of protein catabolic process
E	0045893	biological_process	positive regulation of DNA-templated transcription
E	0048511	biological_process	rhythmic process
E	0050821	biological_process	protein stabilization
E	0051726	biological_process	regulation of cell cycle
E	0051879	molecular_function	Hsp90 protein binding
E	0070822	cellular_component	Sin3-type complex
E	0075342	biological_process	symbiont-mediated disruption of host cell PML body
E	0106310	molecular_function	protein serine kinase activity
E	1901797	biological_process	negative regulation of signal transduction by p53 class mediator
E	1905337	biological_process	positive regulation of aggrephagy
E	1905818	biological_process	regulation of chromosome separation
E	2000042	biological_process	negative regulation of double-strand break repair via homologous recombination
E	2001234	biological_process	negative regulation of apoptotic signaling pathway
F	0000166	molecular_function	nucleotide binding
F	0000785	cellular_component	chromatin
F	0004672	molecular_function	protein kinase activity
F	0004674	molecular_function	protein serine/threonine kinase activity
F	0005515	molecular_function	protein binding
F	0005524	molecular_function	ATP binding
F	0005634	cellular_component	nucleus
F	0005654	cellular_component	nucleoplasm
F	0005829	cellular_component	cytosol
F	0005886	cellular_component	plasma membrane
F	0005956	cellular_component	protein kinase CK2 complex
F	0006302	biological_process	double-strand break repair
F	0006457	biological_process	protein folding
F	0006468	biological_process	protein phosphorylation
F	0006915	biological_process	apoptotic process
F	0006974	biological_process	DNA damage response
F	0007165	biological_process	signal transduction
F	0008284	biological_process	positive regulation of cell population proliferation
F	0016055	biological_process	Wnt signaling pathway
F	0016301	molecular_function	kinase activity
F	0016605	cellular_component	PML body
F	0016740	molecular_function	transferase activity
F	0017148	biological_process	negative regulation of translation
F	0018105	biological_process	peptidyl-serine phosphorylation
F	0030177	biological_process	positive regulation of Wnt signaling pathway
F	0030307	biological_process	positive regulation of cell growth
F	0031507	biological_process	heterochromatin formation
F	0031519	cellular_component	PcG protein complex
F	0032435	biological_process	negative regulation of proteasomal ubiquitin-dependent protein catabolic process
F	0035102	cellular_component	PRC1 complex
F	0042176	biological_process	regulation of protein catabolic process
F	0042802	molecular_function	identical protein binding
F	0045732	biological_process	positive regulation of protein catabolic process
F	0045893	biological_process	positive regulation of DNA-templated transcription
F	0048511	biological_process	rhythmic process
F	0050821	biological_process	protein stabilization
F	0051726	biological_process	regulation of cell cycle
F	0051879	molecular_function	Hsp90 protein binding
F	0070822	cellular_component	Sin3-type complex
F	0075342	biological_process	symbiont-mediated disruption of host cell PML body
F	0106310	molecular_function	protein serine kinase activity
F	1901797	biological_process	negative regulation of signal transduction by p53 class mediator
F	1905337	biological_process	positive regulation of aggrephagy
F	1905818	biological_process	regulation of chromosome separation
F	2000042	biological_process	negative regulation of double-strand break repair via homologous recombination
F	2001234	biological_process	negative regulation of apoptotic signaling pathway
G	0000166	molecular_function	nucleotide binding
G	0000785	cellular_component	chromatin
G	0004672	molecular_function	protein kinase activity
G	0004674	molecular_function	protein serine/threonine kinase activity
G	0005515	molecular_function	protein binding
G	0005524	molecular_function	ATP binding
G	0005634	cellular_component	nucleus
G	0005654	cellular_component	nucleoplasm
G	0005829	cellular_component	cytosol
G	0005886	cellular_component	plasma membrane
G	0005956	cellular_component	protein kinase CK2 complex
G	0006302	biological_process	double-strand break repair
G	0006457	biological_process	protein folding
G	0006468	biological_process	protein phosphorylation
G	0006915	biological_process	apoptotic process
G	0006974	biological_process	DNA damage response
G	0007165	biological_process	signal transduction
G	0008284	biological_process	positive regulation of cell population proliferation
G	0016055	biological_process	Wnt signaling pathway
G	0016301	molecular_function	kinase activity
G	0016605	cellular_component	PML body
G	0016740	molecular_function	transferase activity
G	0017148	biological_process	negative regulation of translation
G	0018105	biological_process	peptidyl-serine phosphorylation
G	0030177	biological_process	positive regulation of Wnt signaling pathway
G	0030307	biological_process	positive regulation of cell growth
G	0031507	biological_process	heterochromatin formation
G	0031519	cellular_component	PcG protein complex
G	0032435	biological_process	negative regulation of proteasomal ubiquitin-dependent protein catabolic process
G	0035102	cellular_component	PRC1 complex
G	0042176	biological_process	regulation of protein catabolic process
G	0042802	molecular_function	identical protein binding
G	0045732	biological_process	positive regulation of protein catabolic process
G	0045893	biological_process	positive regulation of DNA-templated transcription
G	0048511	biological_process	rhythmic process
G	0050821	biological_process	protein stabilization
G	0051726	biological_process	regulation of cell cycle
G	0051879	molecular_function	Hsp90 protein binding
G	0070822	cellular_component	Sin3-type complex
G	0075342	biological_process	symbiont-mediated disruption of host cell PML body
G	0106310	molecular_function	protein serine kinase activity
G	1901797	biological_process	negative regulation of signal transduction by p53 class mediator
G	1905337	biological_process	positive regulation of aggrephagy
G	1905818	biological_process	regulation of chromosome separation
G	2000042	biological_process	negative regulation of double-strand break repair via homologous recombination
G	2001234	biological_process	negative regulation of apoptotic signaling pathway
H	0000166	molecular_function	nucleotide binding
H	0000785	cellular_component	chromatin
H	0004672	molecular_function	protein kinase activity
H	0004674	molecular_function	protein serine/threonine kinase activity
H	0005515	molecular_function	protein binding
H	0005524	molecular_function	ATP binding
H	0005634	cellular_component	nucleus
H	0005654	cellular_component	nucleoplasm
H	0005829	cellular_component	cytosol
H	0005886	cellular_component	plasma membrane
H	0005956	cellular_component	protein kinase CK2 complex
H	0006302	biological_process	double-strand break repair
H	0006457	biological_process	protein folding
H	0006468	biological_process	protein phosphorylation
H	0006915	biological_process	apoptotic process
H	0006974	biological_process	DNA damage response
H	0007165	biological_process	signal transduction
H	0008284	biological_process	positive regulation of cell population proliferation
H	0016055	biological_process	Wnt signaling pathway
H	0016301	molecular_function	kinase activity
H	0016605	cellular_component	PML body
H	0016740	molecular_function	transferase activity
H	0017148	biological_process	negative regulation of translation
H	0018105	biological_process	peptidyl-serine phosphorylation
H	0030177	biological_process	positive regulation of Wnt signaling pathway
H	0030307	biological_process	positive regulation of cell growth
H	0031507	biological_process	heterochromatin formation
H	0031519	cellular_component	PcG protein complex
H	0032435	biological_process	negative regulation of proteasomal ubiquitin-dependent protein catabolic process
H	0035102	cellular_component	PRC1 complex
H	0042176	biological_process	regulation of protein catabolic process
H	0042802	molecular_function	identical protein binding
H	0045732	biological_process	positive regulation of protein catabolic process
H	0045893	biological_process	positive regulation of DNA-templated transcription
H	0048511	biological_process	rhythmic process
H	0050821	biological_process	protein stabilization
H	0051726	biological_process	regulation of cell cycle
H	0051879	molecular_function	Hsp90 protein binding
H	0070822	cellular_component	Sin3-type complex
H	0075342	biological_process	symbiont-mediated disruption of host cell PML body
H	0106310	molecular_function	protein serine kinase activity
H	1901797	biological_process	negative regulation of signal transduction by p53 class mediator
H	1905337	biological_process	positive regulation of aggrephagy
H	1905818	biological_process	regulation of chromosome separation
H	2000042	biological_process	negative regulation of double-strand break repair via homologous recombination
H	2001234	biological_process	negative regulation of apoptotic signaling pathway

Functional Information from PDB Data

site_id	AC1
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN A 301

Chain	Residue
A	CYS109
A	CYS114
A	CYS137
A	CYS140

---

site_id	AC2
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN B 301

Chain	Residue
B	CYS109
B	CYS114
B	CYS137
B	CYS140

---

site_id	AC3
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN C 301

Chain	Residue
C	CYS114
C	CYS137
C	CYS140
C	CYS109

---

site_id	AC4
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN D 301

Chain	Residue
D	CYS109
D	CYS114
D	CYS137
D	CYS140

---

Functional Information from PROSITE/UniProt

site_id	PS00107
Number of Residues	24
Details	PROTEIN_KINASE_ATP Protein kinases ATP-binding region signature. LGRGKYSEVFeAinitnnek..........VVVK

Chain	Residue	Details
E	LEU45-LYS68

---

site_id	PS00108
Number of Residues	13
Details	PROTEIN_KINASE_ST Serine/Threonine protein kinases active-site signature. ImHrDVKphNVMI

Chain	Residue	Details
E	ILE152-ILE164

---

site_id	PS01101
Number of Residues	32
Details	CK2_BETA Casein kinase II regulatory subunit signature. CPrVyCenqpmLPIGlsdipgeamVKlyCPkC

Chain	Residue	Details
A	CYS109-CYS140

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	20
Details	Region: {"description":"Interaction with alpha subunit","evidences":[{"evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	12
Details	Motif: {"description":"KSSR motif","evidences":[{"source":"PubMed","id":"24216761","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	16
Details	Binding site: {}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	8
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"18691976","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	4
Details	Modified residue: {"description":"Phosphothreonine","evidences":[{"source":"PubMed","id":"23186163","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	1140
Details	Domain: {"description":"Protein kinase","evidences":[{"source":"PROSITE-ProRule","id":"PRU00159","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	20
Details	Region: {"description":"Interaction with beta subunit","evidences":[{"evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI8
Number of Residues	4
Details	Active site: {"description":"Proton acceptor"}

Chain

Residue

Details

---

site_id	SWS_FT_FI9
Number of Residues	36
Details	Binding site: {"evidences":[{"source":"PROSITE-ProRule","id":"PRU00159","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---