4M65

In situ thermolysin crystallized on a MiTeGen micromesh with asparagine ligand

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004222	molecular_function	metalloendopeptidase activity
A	0005576	cellular_component	extracellular region
A	0006508	biological_process	proteolysis
A	0008233	molecular_function	peptidase activity
A	0008237	molecular_function	metallopeptidase activity
A	0016787	molecular_function	hydrolase activity
A	0046872	molecular_function	metal ion binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	6
Details	BINDING SITE FOR RESIDUE CA A 401

Chain	Residue
A	ASP138
A	GLU177
A	ASP185
A	GLU187
A	GLU190
A	HOH505

---

site_id	AC2
Number of Residues	6
Details	BINDING SITE FOR RESIDUE CA A 402

Chain	Residue
A	GLU190
A	HOH591
A	HOH701
A	GLU177
A	ASN183
A	ASP185

---

site_id	AC3
Number of Residues	6
Details	BINDING SITE FOR RESIDUE CA A 403

Chain	Residue
A	ASP57
A	ASP59
A	GLN61
A	HOH513
A	HOH521
A	HOH636

---

site_id	AC4
Number of Residues	6
Details	BINDING SITE FOR RESIDUE CA A 404

Chain	Residue
A	TYR193
A	THR194
A	ILE197
A	ASP200
A	EDO416
A	HOH552

---

site_id	AC5
Number of Residues	7
Details	BINDING SITE FOR RESIDUE CA A 405

Chain	Residue
A	TYR211
A	HOH832
A	HOH925
A	HOH926
A	HOH927
A	HOH928
A	HOH931

---

site_id	AC6
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN A 406

Chain	Residue
A	HIS142
A	HIS146
A	GLU166
A	ASN420

---

site_id	AC7
Number of Residues	2
Details	BINDING SITE FOR RESIDUE H2S A 407

Chain	Residue
A	GLN246
A	HOH672

---

site_id	AC8
Number of Residues	1
Details	BINDING SITE FOR RESIDUE H2S A 408

Chain	Residue
A	ARG285

---

site_id	AC9
Number of Residues	7
Details	BINDING SITE FOR RESIDUE EDO A 409

Chain	Residue
A	ASP43
A	SER53
A	LEU54
A	TRP55
A	ALA56
A	LYS219
A	HOH645

---

site_id	BC1
Number of Residues	5
Details	BINDING SITE FOR RESIDUE EDO A 410

Chain	Residue
A	LYS239
A	HIS250
A	HOH507
A	HOH865
A	HOH919

---

site_id	BC2
Number of Residues	2
Details	BINDING SITE FOR RESIDUE EDO A 411

Chain	Residue
A	TYR251
A	HOH614

---

site_id	BC3
Number of Residues	8
Details	BINDING SITE FOR RESIDUE EDO A 412

Chain	Residue
A	TRP115
A	ASP150
A	TYR157
A	EDO414
A	ASN420
A	HOH596
A	HOH888
A	HOH902

---

site_id	BC4
Number of Residues	7
Details	BINDING SITE FOR RESIDUE EDO A 413

Chain	Residue
A	ASN96
A	ASP185
A	GLU187
A	GLU190
A	GLY199
A	HOH638
A	HOH658

---

site_id	BC5
Number of Residues	7
Details	BINDING SITE FOR RESIDUE EDO A 414

Chain	Residue
A	TYR106
A	TRP115
A	ASN116
A	EDO412
A	ASN420
A	HOH673
A	HOH673

---

site_id	BC6
Number of Residues	2
Details	BINDING SITE FOR RESIDUE EDO A 415

Chain	Residue
A	HOH591
A	HOH638

---

site_id	BC7
Number of Residues	10
Details	BINDING SITE FOR RESIDUE EDO A 416

Chain	Residue
A	GLU190
A	ASP191
A	TYR193
A	THR194
A	PRO195
A	ILE197
A	CA404
A	HOH552
A	HOH858
A	HOH924

---

site_id	BC8
Number of Residues	6
Details	BINDING SITE FOR RESIDUE EDO A 417

Chain	Residue
A	THR26
A	ALA58
A	TYR221
A	THR222
A	GLY223
A	HOH775

---

site_id	BC9
Number of Residues	5
Details	BINDING SITE FOR RESIDUE EDO A 418

Chain	Residue
A	ASN112
A	ALA113
A	GLU143
A	ARG203
A	ASN420

---

site_id	CC1
Number of Residues	4
Details	BINDING SITE FOR RESIDUE EDO A 419

Chain	Residue
A	HOH704
A	HOH738
A	GLU160
A	TYR221

---

site_id	CC2
Number of Residues	17
Details	BINDING SITE FOR RESIDUE ASN A 420

Chain	Residue
A	ASN112
A	ALA113
A	PHE114
A	TRP115
A	HIS142
A	GLU143
A	HIS146
A	TYR157
A	GLU166
A	HIS231
A	ZN406
A	EDO412
A	EDO414
A	EDO418
A	HOH830
A	HOH838
A	HOH912

---

Functional Information from PROSITE/UniProt

site_id	PS00142
Number of Residues	10
Details	ZINC_PROTEASE Neutral zinc metallopeptidases, zinc-binding region signature. VVAHELTHAV

Chain	Residue	Details
A	VAL139-VAL148

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