4M4W

Mechanistic implications for the bacterial primosome assembly of the structure of a helicase-helicase loader complex

GO（遺伝子オントロジー）由来の情報

鎖名	GO（遺伝子オントロジー）id	名前空間	内容
A	0003677	molecular_function	DNA binding
A	0003678	molecular_function	DNA helicase activity
A	0004386	molecular_function	helicase activity
A	0005515	molecular_function	protein binding
A	0005524	molecular_function	ATP binding
A	0005829	cellular_component	cytosol
A	0006260	biological_process	DNA replication
A	0006268	biological_process	DNA unwinding involved in DNA replication
A	0006269	biological_process	DNA replication, synthesis of primer
A	0016787	molecular_function	hydrolase activity
A	0016853	molecular_function	isomerase activity
A	0016887	molecular_function	ATP hydrolysis activity
A	0042802	molecular_function	identical protein binding
A	1990077	cellular_component	primosome complex
B	0003677	molecular_function	DNA binding
B	0003678	molecular_function	DNA helicase activity
B	0004386	molecular_function	helicase activity
B	0005515	molecular_function	protein binding
B	0005524	molecular_function	ATP binding
B	0005829	cellular_component	cytosol
B	0006260	biological_process	DNA replication
B	0006268	biological_process	DNA unwinding involved in DNA replication
B	0006269	biological_process	DNA replication, synthesis of primer
B	0016787	molecular_function	hydrolase activity
B	0016853	molecular_function	isomerase activity
B	0016887	molecular_function	ATP hydrolysis activity
B	0042802	molecular_function	identical protein binding
B	1990077	cellular_component	primosome complex
C	0003677	molecular_function	DNA binding
C	0003678	molecular_function	DNA helicase activity
C	0004386	molecular_function	helicase activity
C	0005515	molecular_function	protein binding
C	0005524	molecular_function	ATP binding
C	0005829	cellular_component	cytosol
C	0006260	biological_process	DNA replication
C	0006268	biological_process	DNA unwinding involved in DNA replication
C	0006269	biological_process	DNA replication, synthesis of primer
C	0016787	molecular_function	hydrolase activity
C	0016853	molecular_function	isomerase activity
C	0016887	molecular_function	ATP hydrolysis activity
C	0042802	molecular_function	identical protein binding
C	1990077	cellular_component	primosome complex
D	0003677	molecular_function	DNA binding
D	0003678	molecular_function	DNA helicase activity
D	0004386	molecular_function	helicase activity
D	0005515	molecular_function	protein binding
D	0005524	molecular_function	ATP binding
D	0005829	cellular_component	cytosol
D	0006260	biological_process	DNA replication
D	0006268	biological_process	DNA unwinding involved in DNA replication
D	0006269	biological_process	DNA replication, synthesis of primer
D	0016787	molecular_function	hydrolase activity
D	0016853	molecular_function	isomerase activity
D	0016887	molecular_function	ATP hydrolysis activity
D	0042802	molecular_function	identical protein binding
D	1990077	cellular_component	primosome complex
E	0003677	molecular_function	DNA binding
E	0003678	molecular_function	DNA helicase activity
E	0004386	molecular_function	helicase activity
E	0005515	molecular_function	protein binding
E	0005524	molecular_function	ATP binding
E	0005829	cellular_component	cytosol
E	0006260	biological_process	DNA replication
E	0006268	biological_process	DNA unwinding involved in DNA replication
E	0006269	biological_process	DNA replication, synthesis of primer
E	0016787	molecular_function	hydrolase activity
E	0016853	molecular_function	isomerase activity
E	0016887	molecular_function	ATP hydrolysis activity
E	0042802	molecular_function	identical protein binding
E	1990077	cellular_component	primosome complex
F	0003677	molecular_function	DNA binding
F	0003678	molecular_function	DNA helicase activity
F	0004386	molecular_function	helicase activity
F	0005515	molecular_function	protein binding
F	0005524	molecular_function	ATP binding
F	0005829	cellular_component	cytosol
F	0006260	biological_process	DNA replication
F	0006268	biological_process	DNA unwinding involved in DNA replication
F	0006269	biological_process	DNA replication, synthesis of primer
F	0016787	molecular_function	hydrolase activity
F	0016853	molecular_function	isomerase activity
F	0016887	molecular_function	ATP hydrolysis activity
F	0042802	molecular_function	identical protein binding
F	1990077	cellular_component	primosome complex
G	0003678	molecular_function	DNA helicase activity
G	0005524	molecular_function	ATP binding
G	0006260	biological_process	DNA replication
H	0003678	molecular_function	DNA helicase activity
H	0005524	molecular_function	ATP binding
H	0006260	biological_process	DNA replication
I	0003678	molecular_function	DNA helicase activity
I	0005524	molecular_function	ATP binding
I	0006260	biological_process	DNA replication
J	0003677	molecular_function	DNA binding
J	0005515	molecular_function	protein binding
J	0005524	molecular_function	ATP binding
J	0006260	biological_process	DNA replication
J	0006269	biological_process	DNA replication, synthesis of primer
J	0016787	molecular_function	hydrolase activity
J	0016887	molecular_function	ATP hydrolysis activity
J	0046872	molecular_function	metal ion binding
J	1990077	cellular_component	primosome complex
K	0003677	molecular_function	DNA binding
K	0005515	molecular_function	protein binding
K	0005524	molecular_function	ATP binding
K	0006260	biological_process	DNA replication
K	0006269	biological_process	DNA replication, synthesis of primer
K	0016787	molecular_function	hydrolase activity
K	0016887	molecular_function	ATP hydrolysis activity
K	0046872	molecular_function	metal ion binding
K	1990077	cellular_component	primosome complex
L	0003677	molecular_function	DNA binding
L	0005515	molecular_function	protein binding
L	0005524	molecular_function	ATP binding
L	0006260	biological_process	DNA replication
L	0006269	biological_process	DNA replication, synthesis of primer
L	0016787	molecular_function	hydrolase activity
L	0016887	molecular_function	ATP hydrolysis activity
L	0046872	molecular_function	metal ion binding
L	1990077	cellular_component	primosome complex
M	0003677	molecular_function	DNA binding
M	0005515	molecular_function	protein binding
M	0005524	molecular_function	ATP binding
M	0006260	biological_process	DNA replication
M	0006269	biological_process	DNA replication, synthesis of primer
M	0016787	molecular_function	hydrolase activity
M	0016887	molecular_function	ATP hydrolysis activity
M	0046872	molecular_function	metal ion binding
M	1990077	cellular_component	primosome complex
N	0003677	molecular_function	DNA binding
N	0005515	molecular_function	protein binding
N	0005524	molecular_function	ATP binding
N	0006260	biological_process	DNA replication
N	0006269	biological_process	DNA replication, synthesis of primer
N	0016787	molecular_function	hydrolase activity
N	0016887	molecular_function	ATP hydrolysis activity
N	0046872	molecular_function	metal ion binding
N	1990077	cellular_component	primosome complex
O	0003677	molecular_function	DNA binding
O	0005515	molecular_function	protein binding
O	0005524	molecular_function	ATP binding
O	0006260	biological_process	DNA replication
O	0006269	biological_process	DNA replication, synthesis of primer
O	0016787	molecular_function	hydrolase activity
O	0016887	molecular_function	ATP hydrolysis activity
O	0046872	molecular_function	metal ion binding
O	1990077	cellular_component	primosome complex

UniProtにおけるモチーフ・データベースPROSITEからの機能情報

site_id	PS00039
残基数	9
詳細	DEAD_ATP_HELICASE DEAD-box subfamily ATP-dependent helicases signature. LLDEADRkI

鎖名	残基	詳細
A	LEU139-ILE147

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SwissProt/UniProtに記載されている蛋白質分子機能情報

site_id	SWS_FT_FI1
残基数	6
詳細	ACT_SITE: Nucleophile => ECO:0000305|PubMed:12235389, ECO:0000305|PubMed:23022319

鎖名	残基	詳細
A	GLU241
L	CYS70
L	HIS84
L	CYS101
M	CYS67
M	CYS70
M	HIS84
M	CYS101
N	CYS67
N	CYS70
N	HIS84
B	GLU241
N	CYS101
O	CYS67
O	CYS70
O	HIS84
O	CYS101
C	GLU241
D	GLU241
E	GLU241
F	GLU241
K	HIS84
K	CYS101
L	CYS67

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site_id	SWS_FT_FI2
残基数	54
詳細	BINDING: BINDING => ECO:0000305|PubMed:23022319, ECO:0007744|PDB:4ESV

鎖名	残基	詳細
A	SER213
B	SER213
B	GLY215
B	LYS216
B	THR217
B	ALA218
B	ARG250
B	LYS418
B	GLN419
B	ARG420
C	SER213
A	GLY215
C	GLY215
C	LYS216
C	THR217
C	ALA218
C	ARG250
C	LYS418
C	GLN419
C	ARG420
D	SER213
D	GLY215
A	LYS216
D	LYS216
D	THR217
D	ALA218
D	ARG250
D	LYS418
D	GLN419
D	ARG420
E	SER213
E	GLY215
E	LYS216
A	THR217
E	THR217
E	ALA218
E	ARG250
E	LYS418
E	GLN419
E	ARG420
F	SER213
F	GLY215
F	LYS216
F	THR217
A	ALA218
F	ALA218
F	ARG250
F	LYS418
F	GLN419
F	ARG420
A	ARG250
A	LYS418
A	GLN419
A	ARG420

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site_id	SWS_FT_FI3
残基数	6
詳細	BINDING: BINDING => ECO:0000305|PubMed:23022319

鎖名	残基	詳細
A	GLN362
B	GLN362
C	GLN362
D	GLN362
E	GLN362
F	GLN362

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site_id	SWS_FT_FI4
残基数	18
詳細	BINDING: BINDING => ECO:0000269|PubMed:23022319, ECO:0007744|PDB:4ESV

鎖名	残基	詳細
A	ARG381
D	ARG381
D	GLU382
D	GLY384
E	ARG381
E	GLU382
E	GLY384
F	ARG381
F	GLU382
F	GLY384
A	GLU382
A	GLY384
B	ARG381
B	GLU382
B	GLY384
C	ARG381
C	GLU382
C	GLY384

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site_id	SWS_FT_FI5
残基数	6
詳細	SITE: Gamma-phosphate sensor => ECO:0000305|PubMed:12235389

鎖名	残基	詳細
A	GLN362
B	GLN362
C	GLN362
D	GLN362
E	GLN362
F	GLN362

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