4LNO
B. subtilis glutamine synthetase structures reveal large active site conformational changes and basis for isoenzyme specific regulation: form two of GS-1
Functional Information from GO Data
Chain | GOid | namespace | contents |
A | 0000287 | molecular_function | magnesium ion binding |
A | 0003824 | molecular_function | catalytic activity |
A | 0004356 | molecular_function | glutamine synthetase activity |
A | 0005515 | molecular_function | protein binding |
A | 0005524 | molecular_function | ATP binding |
A | 0005737 | cellular_component | cytoplasm |
A | 0006542 | biological_process | glutamine biosynthetic process |
A | 0016595 | molecular_function | glutamate binding |
A | 0016874 | molecular_function | ligase activity |
A | 0043562 | biological_process | cellular response to nitrogen levels |
A | 0045892 | biological_process | negative regulation of DNA-templated transcription |
A | 0046872 | molecular_function | metal ion binding |
A | 0070406 | molecular_function | glutamine binding |
A | 0090295 | biological_process | nitrogen catabolite repression of transcription |
A | 0140297 | molecular_function | DNA-binding transcription factor binding |
A | 1904797 | biological_process | negative regulation of core promoter binding |
B | 0000287 | molecular_function | magnesium ion binding |
B | 0003824 | molecular_function | catalytic activity |
B | 0004356 | molecular_function | glutamine synthetase activity |
B | 0005515 | molecular_function | protein binding |
B | 0005524 | molecular_function | ATP binding |
B | 0005737 | cellular_component | cytoplasm |
B | 0006542 | biological_process | glutamine biosynthetic process |
B | 0016595 | molecular_function | glutamate binding |
B | 0016874 | molecular_function | ligase activity |
B | 0043562 | biological_process | cellular response to nitrogen levels |
B | 0045892 | biological_process | negative regulation of DNA-templated transcription |
B | 0046872 | molecular_function | metal ion binding |
B | 0070406 | molecular_function | glutamine binding |
B | 0090295 | biological_process | nitrogen catabolite repression of transcription |
B | 0140297 | molecular_function | DNA-binding transcription factor binding |
B | 1904797 | biological_process | negative regulation of core promoter binding |
C | 0000287 | molecular_function | magnesium ion binding |
C | 0003824 | molecular_function | catalytic activity |
C | 0004356 | molecular_function | glutamine synthetase activity |
C | 0005515 | molecular_function | protein binding |
C | 0005524 | molecular_function | ATP binding |
C | 0005737 | cellular_component | cytoplasm |
C | 0006542 | biological_process | glutamine biosynthetic process |
C | 0016595 | molecular_function | glutamate binding |
C | 0016874 | molecular_function | ligase activity |
C | 0043562 | biological_process | cellular response to nitrogen levels |
C | 0045892 | biological_process | negative regulation of DNA-templated transcription |
C | 0046872 | molecular_function | metal ion binding |
C | 0070406 | molecular_function | glutamine binding |
C | 0090295 | biological_process | nitrogen catabolite repression of transcription |
C | 0140297 | molecular_function | DNA-binding transcription factor binding |
C | 1904797 | biological_process | negative regulation of core promoter binding |
D | 0000287 | molecular_function | magnesium ion binding |
D | 0003824 | molecular_function | catalytic activity |
D | 0004356 | molecular_function | glutamine synthetase activity |
D | 0005515 | molecular_function | protein binding |
D | 0005524 | molecular_function | ATP binding |
D | 0005737 | cellular_component | cytoplasm |
D | 0006542 | biological_process | glutamine biosynthetic process |
D | 0016595 | molecular_function | glutamate binding |
D | 0016874 | molecular_function | ligase activity |
D | 0043562 | biological_process | cellular response to nitrogen levels |
D | 0045892 | biological_process | negative regulation of DNA-templated transcription |
D | 0046872 | molecular_function | metal ion binding |
D | 0070406 | molecular_function | glutamine binding |
D | 0090295 | biological_process | nitrogen catabolite repression of transcription |
D | 0140297 | molecular_function | DNA-binding transcription factor binding |
D | 1904797 | biological_process | negative regulation of core promoter binding |
E | 0000287 | molecular_function | magnesium ion binding |
E | 0003824 | molecular_function | catalytic activity |
E | 0004356 | molecular_function | glutamine synthetase activity |
E | 0005515 | molecular_function | protein binding |
E | 0005524 | molecular_function | ATP binding |
E | 0005737 | cellular_component | cytoplasm |
E | 0006542 | biological_process | glutamine biosynthetic process |
E | 0016595 | molecular_function | glutamate binding |
E | 0016874 | molecular_function | ligase activity |
E | 0043562 | biological_process | cellular response to nitrogen levels |
E | 0045892 | biological_process | negative regulation of DNA-templated transcription |
E | 0046872 | molecular_function | metal ion binding |
E | 0070406 | molecular_function | glutamine binding |
E | 0090295 | biological_process | nitrogen catabolite repression of transcription |
E | 0140297 | molecular_function | DNA-binding transcription factor binding |
E | 1904797 | biological_process | negative regulation of core promoter binding |
F | 0000287 | molecular_function | magnesium ion binding |
F | 0003824 | molecular_function | catalytic activity |
F | 0004356 | molecular_function | glutamine synthetase activity |
F | 0005515 | molecular_function | protein binding |
F | 0005524 | molecular_function | ATP binding |
F | 0005737 | cellular_component | cytoplasm |
F | 0006542 | biological_process | glutamine biosynthetic process |
F | 0016595 | molecular_function | glutamate binding |
F | 0016874 | molecular_function | ligase activity |
F | 0043562 | biological_process | cellular response to nitrogen levels |
F | 0045892 | biological_process | negative regulation of DNA-templated transcription |
F | 0046872 | molecular_function | metal ion binding |
F | 0070406 | molecular_function | glutamine binding |
F | 0090295 | biological_process | nitrogen catabolite repression of transcription |
F | 0140297 | molecular_function | DNA-binding transcription factor binding |
F | 1904797 | biological_process | negative regulation of core promoter binding |
Functional Information from PDB Data
site_id | AC1 |
Number of Residues | 5 |
Details | BINDING SITE FOR RESIDUE MG A 501 |
Chain | Residue |
A | GLU134 |
A | GLU189 |
A | GLU196 |
A | GLN503 |
A | HOH608 |
site_id | AC2 |
Number of Residues | 5 |
Details | BINDING SITE FOR RESIDUE MG A 502 |
Chain | Residue |
B | GLU65 |
A | GLU132 |
A | HIS245 |
A | GLU333 |
A | HOH608 |
site_id | AC3 |
Number of Residues | 12 |
Details | BINDING SITE FOR RESIDUE GLN A 503 |
Chain | Residue |
A | GLU134 |
A | TYR156 |
A | GLU189 |
A | GLN194 |
A | GLY241 |
A | HIS245 |
A | ARG298 |
A | GLU304 |
A | MG501 |
A | HOH608 |
A | HOH609 |
A | HOH663 |
site_id | AC4 |
Number of Residues | 5 |
Details | BINDING SITE FOR RESIDUE MG B 501 |
Chain | Residue |
B | GLU134 |
B | GLU189 |
B | GLU196 |
B | GLN503 |
B | HOH686 |
site_id | AC5 |
Number of Residues | 3 |
Details | BINDING SITE FOR RESIDUE MG B 502 |
Chain | Residue |
B | GLU132 |
B | HIS245 |
B | GLU333 |
site_id | AC6 |
Number of Residues | 12 |
Details | BINDING SITE FOR RESIDUE GLN B 503 |
Chain | Residue |
B | GLU134 |
B | TYR156 |
B | GLU189 |
B | GLY241 |
B | HIS245 |
B | ARG298 |
B | GLU304 |
B | ALA305 |
B | ARG335 |
B | MG501 |
B | HOH604 |
C | HOH607 |
site_id | AC7 |
Number of Residues | 10 |
Details | BINDING SITE FOR RESIDUE GLN C 501 |
Chain | Residue |
C | GLU134 |
C | TYR156 |
C | GLU189 |
C | GLY241 |
C | HIS245 |
C | ARG298 |
C | GLU304 |
C | MG503 |
C | HOH603 |
C | HOH628 |
site_id | AC8 |
Number of Residues | 4 |
Details | BINDING SITE FOR RESIDUE MG C 502 |
Chain | Residue |
C | GLU132 |
C | HIS245 |
C | GLU333 |
D | GLU65 |
site_id | AC9 |
Number of Residues | 4 |
Details | BINDING SITE FOR RESIDUE MG C 503 |
Chain | Residue |
C | GLU134 |
C | GLU189 |
C | GLU196 |
C | GLN501 |
site_id | BC1 |
Number of Residues | 4 |
Details | BINDING SITE FOR RESIDUE MG D 501 |
Chain | Residue |
D | GLU134 |
D | GLU189 |
D | GLU196 |
D | GLN503 |
site_id | BC2 |
Number of Residues | 5 |
Details | BINDING SITE FOR RESIDUE MG D 502 |
Chain | Residue |
D | GLU132 |
D | HIS245 |
D | GLU333 |
D | HOH704 |
E | GLU65 |
site_id | BC3 |
Number of Residues | 9 |
Details | BINDING SITE FOR RESIDUE GLN D 503 |
Chain | Residue |
D | GLU134 |
D | TYR156 |
D | GLU189 |
D | GLY241 |
D | HIS245 |
D | ARG298 |
D | GLU304 |
D | MG501 |
D | HOH611 |
site_id | BC4 |
Number of Residues | 6 |
Details | BINDING SITE FOR RESIDUE MG E 501 |
Chain | Residue |
E | GLU132 |
E | HIS245 |
E | GLU333 |
E | HOH627 |
F | GLU65 |
F | HOH602 |
site_id | BC5 |
Number of Residues | 4 |
Details | BINDING SITE FOR RESIDUE MG E 502 |
Chain | Residue |
E | GLU134 |
E | GLU189 |
E | GLU196 |
E | GLN503 |
site_id | BC6 |
Number of Residues | 10 |
Details | BINDING SITE FOR RESIDUE GLN E 503 |
Chain | Residue |
E | GLU134 |
E | TYR156 |
E | GLU189 |
E | ASN240 |
E | GLY241 |
E | HIS245 |
E | ARG298 |
E | GLU304 |
E | MG502 |
E | HOH636 |
site_id | BC7 |
Number of Residues | 3 |
Details | BINDING SITE FOR RESIDUE MG F 501 |
Chain | Residue |
F | HIS245 |
F | GLU333 |
F | GLU132 |
site_id | BC8 |
Number of Residues | 4 |
Details | BINDING SITE FOR RESIDUE MG F 502 |
Chain | Residue |
F | GLU134 |
F | GLU189 |
F | GLU196 |
F | GLN503 |
site_id | BC9 |
Number of Residues | 9 |
Details | BINDING SITE FOR RESIDUE GLN F 503 |
Chain | Residue |
F | GLU134 |
F | GLU189 |
F | GLY241 |
F | HIS245 |
F | ARG298 |
F | GLU304 |
F | MG502 |
F | HOH612 |
F | HOH659 |
Functional Information from PROSITE/UniProt
site_id | PS00180 |
Number of Residues | 19 |
Details | GLNA_1 Glutamine synthetase signature 1. FDGSSiegfvrieESDmyL |
Chain | Residue | Details |
A | PHE52-LEU70 |
site_id | PS00181 |
Number of Residues | 16 |
Details | GLNA_ATP Glutamine synthetase putative ATP-binding region signature. KPLfgv..NGSGmHcnlS |
Chain | Residue | Details |
A | LYS234-SER249 |
site_id | PS00182 |
Number of Residues | 13 |
Details | GLNA_ADENYLATION Glutamine synthetase class-I adenylation site. KLeapapIDRNIY |
Chain | Residue | Details |
A | LYS361-TYR373 |
Functional Information from SwissProt/UniProt
site_id | SWS_FT_FI1 |
Number of Residues | 12 |
Details | BINDING: BINDING => ECO:0000269|PubMed:24158439, ECO:0000269|PubMed:25691471, ECO:0007744|PDB:4LNF, ECO:0007744|PDB:4LNI, ECO:0007744|PDB:4LNK, ECO:0007744|PDB:4LNN, ECO:0007744|PDB:4S0R |
Chain | Residue | Details |
A | GLU132 | |
E | GLU333 | |
F | GLU132 | |
F | GLU333 | |
A | GLU333 | |
B | GLU132 | |
B | GLU333 | |
C | GLU132 | |
C | GLU333 | |
D | GLU132 | |
D | GLU333 | |
E | GLU132 |
site_id | SWS_FT_FI2 |
Number of Residues | 18 |
Details | BINDING: BINDING => ECO:0000269|PubMed:24158439, ECO:0000269|PubMed:25691471, ECO:0007744|PDB:4LNF, ECO:0007744|PDB:4LNI, ECO:0007744|PDB:4LNK, ECO:0007744|PDB:4S0R |
Chain | Residue | Details |
A | GLU134 | |
D | GLU134 | |
D | GLU189 | |
D | GLU196 | |
E | GLU134 | |
E | GLU189 | |
E | GLU196 | |
F | GLU134 | |
F | GLU189 | |
F | GLU196 | |
A | GLU189 | |
A | GLU196 | |
B | GLU134 | |
B | GLU189 | |
B | GLU196 | |
C | GLU134 | |
C | GLU189 | |
C | GLU196 |
site_id | SWS_FT_FI3 |
Number of Residues | 24 |
Details | BINDING: BINDING => ECO:0000250|UniProtKB:P9WN39 |
Chain | Residue | Details |
A | GLU184 | |
C | ASN240 | |
C | ARG316 | |
C | ARG321 | |
D | GLU184 | |
D | ASN240 | |
D | ARG316 | |
D | ARG321 | |
E | GLU184 | |
E | ASN240 | |
E | ARG316 | |
A | ASN240 | |
E | ARG321 | |
F | GLU184 | |
F | ASN240 | |
F | ARG316 | |
F | ARG321 | |
A | ARG316 | |
A | ARG321 | |
B | GLU184 | |
B | ASN240 | |
B | ARG316 | |
B | ARG321 | |
C | GLU184 |
site_id | SWS_FT_FI4 |
Number of Residues | 6 |
Details | BINDING: BINDING => ECO:0000269|PubMed:24158439, ECO:0000269|PubMed:25691471, ECO:0007744|PDB:4LNF, ECO:0007744|PDB:4LNK, ECO:0007744|PDB:4LNN, ECO:0007744|PDB:4S0R |
Chain | Residue | Details |
A | GLY241 | |
B | GLY241 | |
C | GLY241 | |
D | GLY241 | |
E | GLY241 | |
F | GLY241 |
site_id | SWS_FT_FI5 |
Number of Residues | 6 |
Details | BINDING: BINDING => ECO:0000269|PubMed:24158439, ECO:0000269|PubMed:25691471, ECO:0007744|PDB:4LNF, ECO:0007744|PDB:4LNK, ECO:0007744|PDB:4S0R |
Chain | Residue | Details |
A | HIS245 | |
B | HIS245 | |
C | HIS245 | |
D | HIS245 | |
E | HIS245 | |
F | HIS245 |
site_id | SWS_FT_FI6 |
Number of Residues | 6 |
Details | BINDING: BINDING => ECO:0000250|UniProtKB:P77961 |
Chain | Residue | Details |
A | SER249 | |
B | SER249 | |
C | SER249 | |
D | SER249 | |
E | SER249 | |
F | SER249 |
site_id | SWS_FT_FI7 |
Number of Residues | 18 |
Details | BINDING: BINDING => ECO:0000250|UniProtKB:P0A1P6 |
Chain | Residue | Details |
A | ARG298 | |
D | ARG298 | |
D | GLU304 | |
D | ARG335 | |
E | ARG298 | |
E | GLU304 | |
E | ARG335 | |
F | ARG298 | |
F | GLU304 | |
F | ARG335 | |
A | GLU304 | |
A | ARG335 | |
B | ARG298 | |
B | GLU304 | |
B | ARG335 | |
C | ARG298 | |
C | GLU304 | |
C | ARG335 |
site_id | SWS_FT_FI8 |
Number of Residues | 6 |
Details | SITE: Important for inhibition by glutamine => ECO:0000269|PubMed:24158439 |
Chain | Residue | Details |
A | ARG62 | |
B | ARG62 | |
C | ARG62 | |
D | ARG62 | |
E | ARG62 | |
F | ARG62 |