Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

4LNN

B. subtilis glutamine synthetase structures reveal large active site conformational changes and basis for isoenzyme specific regulation: structure of apo form of GS

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000287	molecular_function	magnesium ion binding
A	0003824	molecular_function	catalytic activity
A	0004356	molecular_function	glutamine synthetase activity
A	0005515	molecular_function	protein binding
A	0005524	molecular_function	ATP binding
A	0005737	cellular_component	cytoplasm
A	0006542	biological_process	glutamine biosynthetic process
A	0006807	biological_process	obsolete nitrogen compound metabolic process
A	0016595	molecular_function	glutamate binding
A	0016874	molecular_function	ligase activity
A	0043562	biological_process	cellular response to nitrogen levels
A	0045892	biological_process	negative regulation of DNA-templated transcription
A	0046872	molecular_function	metal ion binding
A	0070406	molecular_function	glutamine binding
A	0090295	biological_process	nitrogen catabolite repression of transcription
A	0140297	molecular_function	DNA-binding transcription factor binding
A	1904797	biological_process	negative regulation of core promoter binding
B	0000287	molecular_function	magnesium ion binding
B	0003824	molecular_function	catalytic activity
B	0004356	molecular_function	glutamine synthetase activity
B	0005515	molecular_function	protein binding
B	0005524	molecular_function	ATP binding
B	0005737	cellular_component	cytoplasm
B	0006542	biological_process	glutamine biosynthetic process
B	0006807	biological_process	obsolete nitrogen compound metabolic process
B	0016595	molecular_function	glutamate binding
B	0016874	molecular_function	ligase activity
B	0043562	biological_process	cellular response to nitrogen levels
B	0045892	biological_process	negative regulation of DNA-templated transcription
B	0046872	molecular_function	metal ion binding
B	0070406	molecular_function	glutamine binding
B	0090295	biological_process	nitrogen catabolite repression of transcription
B	0140297	molecular_function	DNA-binding transcription factor binding
B	1904797	biological_process	negative regulation of core promoter binding
C	0000287	molecular_function	magnesium ion binding
C	0003824	molecular_function	catalytic activity
C	0004356	molecular_function	glutamine synthetase activity
C	0005515	molecular_function	protein binding
C	0005524	molecular_function	ATP binding
C	0005737	cellular_component	cytoplasm
C	0006542	biological_process	glutamine biosynthetic process
C	0006807	biological_process	obsolete nitrogen compound metabolic process
C	0016595	molecular_function	glutamate binding
C	0016874	molecular_function	ligase activity
C	0043562	biological_process	cellular response to nitrogen levels
C	0045892	biological_process	negative regulation of DNA-templated transcription
C	0046872	molecular_function	metal ion binding
C	0070406	molecular_function	glutamine binding
C	0090295	biological_process	nitrogen catabolite repression of transcription
C	0140297	molecular_function	DNA-binding transcription factor binding
C	1904797	biological_process	negative regulation of core promoter binding
D	0000287	molecular_function	magnesium ion binding
D	0003824	molecular_function	catalytic activity
D	0004356	molecular_function	glutamine synthetase activity
D	0005515	molecular_function	protein binding
D	0005524	molecular_function	ATP binding
D	0005737	cellular_component	cytoplasm
D	0006542	biological_process	glutamine biosynthetic process
D	0006807	biological_process	obsolete nitrogen compound metabolic process
D	0016595	molecular_function	glutamate binding
D	0016874	molecular_function	ligase activity
D	0043562	biological_process	cellular response to nitrogen levels
D	0045892	biological_process	negative regulation of DNA-templated transcription
D	0046872	molecular_function	metal ion binding
D	0070406	molecular_function	glutamine binding
D	0090295	biological_process	nitrogen catabolite repression of transcription
D	0140297	molecular_function	DNA-binding transcription factor binding
D	1904797	biological_process	negative regulation of core promoter binding
E	0000287	molecular_function	magnesium ion binding
E	0003824	molecular_function	catalytic activity
E	0004356	molecular_function	glutamine synthetase activity
E	0005515	molecular_function	protein binding
E	0005524	molecular_function	ATP binding
E	0005737	cellular_component	cytoplasm
E	0006542	biological_process	glutamine biosynthetic process
E	0006807	biological_process	obsolete nitrogen compound metabolic process
E	0016595	molecular_function	glutamate binding
E	0016874	molecular_function	ligase activity
E	0043562	biological_process	cellular response to nitrogen levels
E	0045892	biological_process	negative regulation of DNA-templated transcription
E	0046872	molecular_function	metal ion binding
E	0070406	molecular_function	glutamine binding
E	0090295	biological_process	nitrogen catabolite repression of transcription
E	0140297	molecular_function	DNA-binding transcription factor binding
E	1904797	biological_process	negative regulation of core promoter binding
F	0000287	molecular_function	magnesium ion binding
F	0003824	molecular_function	catalytic activity
F	0004356	molecular_function	glutamine synthetase activity
F	0005515	molecular_function	protein binding
F	0005524	molecular_function	ATP binding
F	0005737	cellular_component	cytoplasm
F	0006542	biological_process	glutamine biosynthetic process
F	0006807	biological_process	obsolete nitrogen compound metabolic process
F	0016595	molecular_function	glutamate binding
F	0016874	molecular_function	ligase activity
F	0043562	biological_process	cellular response to nitrogen levels
F	0045892	biological_process	negative regulation of DNA-templated transcription
F	0046872	molecular_function	metal ion binding
F	0070406	molecular_function	glutamine binding
F	0090295	biological_process	nitrogen catabolite repression of transcription
F	0140297	molecular_function	DNA-binding transcription factor binding
F	1904797	biological_process	negative regulation of core promoter binding
G	0000287	molecular_function	magnesium ion binding
G	0003824	molecular_function	catalytic activity
G	0004356	molecular_function	glutamine synthetase activity
G	0005515	molecular_function	protein binding
G	0005524	molecular_function	ATP binding
G	0005737	cellular_component	cytoplasm
G	0006542	biological_process	glutamine biosynthetic process
G	0006807	biological_process	obsolete nitrogen compound metabolic process
G	0016595	molecular_function	glutamate binding
G	0016874	molecular_function	ligase activity
G	0043562	biological_process	cellular response to nitrogen levels
G	0045892	biological_process	negative regulation of DNA-templated transcription
G	0046872	molecular_function	metal ion binding
G	0070406	molecular_function	glutamine binding
G	0090295	biological_process	nitrogen catabolite repression of transcription
G	0140297	molecular_function	DNA-binding transcription factor binding
G	1904797	biological_process	negative regulation of core promoter binding
H	0000287	molecular_function	magnesium ion binding
H	0003824	molecular_function	catalytic activity
H	0004356	molecular_function	glutamine synthetase activity
H	0005515	molecular_function	protein binding
H	0005524	molecular_function	ATP binding
H	0005737	cellular_component	cytoplasm
H	0006542	biological_process	glutamine biosynthetic process
H	0006807	biological_process	obsolete nitrogen compound metabolic process
H	0016595	molecular_function	glutamate binding
H	0016874	molecular_function	ligase activity
H	0043562	biological_process	cellular response to nitrogen levels
H	0045892	biological_process	negative regulation of DNA-templated transcription
H	0046872	molecular_function	metal ion binding
H	0070406	molecular_function	glutamine binding
H	0090295	biological_process	nitrogen catabolite repression of transcription
H	0140297	molecular_function	DNA-binding transcription factor binding
H	1904797	biological_process	negative regulation of core promoter binding
I	0000287	molecular_function	magnesium ion binding
I	0003824	molecular_function	catalytic activity
I	0004356	molecular_function	glutamine synthetase activity
I	0005515	molecular_function	protein binding
I	0005524	molecular_function	ATP binding
I	0005737	cellular_component	cytoplasm
I	0006542	biological_process	glutamine biosynthetic process
I	0006807	biological_process	obsolete nitrogen compound metabolic process
I	0016595	molecular_function	glutamate binding
I	0016874	molecular_function	ligase activity
I	0043562	biological_process	cellular response to nitrogen levels
I	0045892	biological_process	negative regulation of DNA-templated transcription
I	0046872	molecular_function	metal ion binding
I	0070406	molecular_function	glutamine binding
I	0090295	biological_process	nitrogen catabolite repression of transcription
I	0140297	molecular_function	DNA-binding transcription factor binding
I	1904797	biological_process	negative regulation of core promoter binding
J	0000287	molecular_function	magnesium ion binding
J	0003824	molecular_function	catalytic activity
J	0004356	molecular_function	glutamine synthetase activity
J	0005515	molecular_function	protein binding
J	0005524	molecular_function	ATP binding
J	0005737	cellular_component	cytoplasm
J	0006542	biological_process	glutamine biosynthetic process
J	0006807	biological_process	obsolete nitrogen compound metabolic process
J	0016595	molecular_function	glutamate binding
J	0016874	molecular_function	ligase activity
J	0043562	biological_process	cellular response to nitrogen levels
J	0045892	biological_process	negative regulation of DNA-templated transcription
J	0046872	molecular_function	metal ion binding
J	0070406	molecular_function	glutamine binding
J	0090295	biological_process	nitrogen catabolite repression of transcription
J	0140297	molecular_function	DNA-binding transcription factor binding
J	1904797	biological_process	negative regulation of core promoter binding
K	0000287	molecular_function	magnesium ion binding
K	0003824	molecular_function	catalytic activity
K	0004356	molecular_function	glutamine synthetase activity
K	0005515	molecular_function	protein binding
K	0005524	molecular_function	ATP binding
K	0005737	cellular_component	cytoplasm
K	0006542	biological_process	glutamine biosynthetic process
K	0006807	biological_process	obsolete nitrogen compound metabolic process
K	0016595	molecular_function	glutamate binding
K	0016874	molecular_function	ligase activity
K	0043562	biological_process	cellular response to nitrogen levels
K	0045892	biological_process	negative regulation of DNA-templated transcription
K	0046872	molecular_function	metal ion binding
K	0070406	molecular_function	glutamine binding
K	0090295	biological_process	nitrogen catabolite repression of transcription
K	0140297	molecular_function	DNA-binding transcription factor binding
K	1904797	biological_process	negative regulation of core promoter binding
L	0000287	molecular_function	magnesium ion binding
L	0003824	molecular_function	catalytic activity
L	0004356	molecular_function	glutamine synthetase activity
L	0005515	molecular_function	protein binding
L	0005524	molecular_function	ATP binding
L	0005737	cellular_component	cytoplasm
L	0006542	biological_process	glutamine biosynthetic process
L	0006807	biological_process	obsolete nitrogen compound metabolic process
L	0016595	molecular_function	glutamate binding
L	0016874	molecular_function	ligase activity
L	0043562	biological_process	cellular response to nitrogen levels
L	0045892	biological_process	negative regulation of DNA-templated transcription
L	0046872	molecular_function	metal ion binding
L	0070406	molecular_function	glutamine binding
L	0090295	biological_process	nitrogen catabolite repression of transcription
L	0140297	molecular_function	DNA-binding transcription factor binding
L	1904797	biological_process	negative regulation of core promoter binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	3
Details	BINDING SITE FOR RESIDUE MG A 501

Chain	Residue
A	GLU134
A	GLU189
A	GLU196

site_id	AC2
Number of Residues	3
Details	BINDING SITE FOR RESIDUE MG A 502

Chain	Residue
A	GLU132
A	HIS245
A	GLU333

site_id	AC3
Number of Residues	4
Details	BINDING SITE FOR RESIDUE MG B 501

Chain	Residue
B	HOH610
B	GLU134
B	GLU189
B	GLU196

site_id	AC4
Number of Residues	2
Details	BINDING SITE FOR RESIDUE MG B 502

Chain	Residue
B	GLU132
B	GLU333

site_id	AC5
Number of Residues	2
Details	BINDING SITE FOR RESIDUE MG B 503

Chain	Residue
B	VAL61
B	HIS416

site_id	AC6
Number of Residues	3
Details	BINDING SITE FOR RESIDUE MG C 501

Chain	Residue
C	GLU134
C	GLU189
C	GLU196

site_id	AC7
Number of Residues	2
Details	BINDING SITE FOR RESIDUE MG C 502

Chain	Residue
C	GLU132
C	GLU333

site_id	AC8
Number of Residues	3
Details	BINDING SITE FOR RESIDUE SO4 D 501

Chain	Residue
D	VAL78
D	ARG206
D	ASP210

site_id	AC9
Number of Residues	3
Details	BINDING SITE FOR RESIDUE MG D 502

Chain	Residue
D	GLU134
D	GLU189
D	GLU196

site_id	BC1
Number of Residues	3
Details	BINDING SITE FOR RESIDUE MG D 503

Chain	Residue
D	GLU132
D	HIS245
D	GLU333

site_id	BC2
Number of Residues	3
Details	BINDING SITE FOR RESIDUE SO4 D 504

Chain	Residue
C	LYS224
D	GLU85
D	LYS86

site_id	BC3
Number of Residues	3
Details	BINDING SITE FOR RESIDUE MG E 501

Chain	Residue
E	GLU132
E	ARG321
E	GLU333

site_id	BC4
Number of Residues	3
Details	BINDING SITE FOR RESIDUE MG E 502

Chain	Residue
E	GLU134
E	GLU189
E	GLU196

site_id	BC5
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 E 503

Chain	Residue
E	THR83
E	ALA84
E	GLY87
E	LYS88

site_id	BC6
Number of Residues	5
Details	BINDING SITE FOR RESIDUE MG F 501

Chain	Residue
A	GLU65
F	GLU132
F	HIS245
F	ARG321
F	GLU333

site_id	BC7
Number of Residues	5
Details	BINDING SITE FOR RESIDUE MG F 502

Chain	Residue
F	GLU134
F	HIS187
F	GLU189
F	GLU196
F	HOH610

site_id	BC8
Number of Residues	3
Details	BINDING SITE FOR RESIDUE SO4 F 503

Chain	Residue
F	THR83
F	GLU85
F	LYS88

site_id	BC9
Number of Residues	3
Details	BINDING SITE FOR RESIDUE MG G 501

Chain	Residue
G	GLU134
G	GLU189
G	GLU196

site_id	CC1
Number of Residues	2
Details	BINDING SITE FOR RESIDUE MG G 502

Chain	Residue
G	GLU132
G	GLU333

site_id	CC2
Number of Residues	5
Details	BINDING SITE FOR RESIDUE SO4 H 501

Chain	Residue
H	THR83
H	ALA84
H	GLU85
H	GLY87
H	LYS88

site_id	CC3
Number of Residues	3
Details	BINDING SITE FOR RESIDUE MG H 502

Chain	Residue
H	GLU134
H	GLU189
H	GLU196

site_id	CC4
Number of Residues	2
Details	BINDING SITE FOR RESIDUE MG H 503

Chain	Residue
H	GLU132
H	GLU333

site_id	CC5
Number of Residues	3
Details	BINDING SITE FOR RESIDUE MG I 501

Chain	Residue
I	GLU134
I	GLU189
I	GLU196

site_id	CC6
Number of Residues	4
Details	BINDING SITE FOR RESIDUE MG I 502

Chain	Residue
H	GLU65
I	GLU132
I	ARG321
I	GLU333

site_id	CC7
Number of Residues	3
Details	BINDING SITE FOR RESIDUE MG J 501

Chain	Residue
J	GLU134
J	GLU189
J	GLU196

site_id	CC8
Number of Residues	2
Details	BINDING SITE FOR RESIDUE MG J 502

Chain	Residue
J	GLU132
J	GLU333

site_id	CC9
Number of Residues	3
Details	BINDING SITE FOR RESIDUE MG K 501

Chain	Residue
K	GLU134
K	GLU189
K	GLU196

site_id	DC1
Number of Residues	3
Details	BINDING SITE FOR RESIDUE MG K 502

Chain	Residue
K	GLU132
K	ARG321
K	GLU333

site_id	DC2
Number of Residues	3
Details	BINDING SITE FOR RESIDUE MG L 501

Chain	Residue
L	GLU134
L	GLU189
L	GLU196

site_id	DC3
Number of Residues	2
Details	BINDING SITE FOR RESIDUE MG L 502

Chain	Residue
L	GLU132
L	GLU333

Functional Information from PROSITE/UniProt

site_id	PS00180
Number of Residues	19
Details	GLNA_1 Glutamine synthetase signature 1. FDGSSiegfvrieESDmyL

Chain	Residue	Details
A	PHE52-LEU70

site_id	PS00181
Number of Residues	16
Details	GLNA_ATP Glutamine synthetase putative ATP-binding region signature. KPLfgv..NGSGmHcnlS

Chain	Residue	Details
A	LYS234-SER249

site_id	PS00182
Number of Residues	13
Details	GLNA_ADENYLATION Glutamine synthetase class-I adenylation site. KLeapapIDRNIY

Chain	Residue	Details
A	LYS361-TYR373

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	24
Details	BINDING: BINDING => ECO:0000269\|PubMed:24158439, ECO:0000269\|PubMed:25691471, ECO:0007744\|PDB:4LNF, ECO:0007744\|PDB:4LNI, ECO:0007744\|PDB:4LNK, ECO:0007744\|PDB:4LNN, ECO:0007744\|PDB:4S0R

Chain	Residue	Details
A	GLU132
A	GLU333
B	GLU132
B	GLU333
C	GLU132
C	GLU333
D	GLU132
D	GLU333
E	GLU132
E	GLU333
F	GLU132
F	GLU333
G	GLU132
G	GLU333
H	GLU132
H	GLU333
I	GLU132
I	GLU333
J	GLU132
J	GLU333
K	GLU132
K	GLU333
L	GLU132
L	GLU333

site_id	SWS_FT_FI2
Number of Residues	36
Details	BINDING: BINDING => ECO:0000269\|PubMed:24158439, ECO:0000269\|PubMed:25691471, ECO:0007744\|PDB:4LNF, ECO:0007744\|PDB:4LNI, ECO:0007744\|PDB:4LNK, ECO:0007744\|PDB:4S0R

Chain	Residue	Details
A	GLU134
A	GLU189
A	GLU196
B	GLU134
B	GLU189
B	GLU196
C	GLU134
C	GLU189
C	GLU196
D	GLU134
D	GLU189
D	GLU196
E	GLU134
E	GLU189
E	GLU196
F	GLU134
F	GLU189
F	GLU196
G	GLU134
G	GLU189
G	GLU196
H	GLU134
H	GLU189
H	GLU196
I	GLU134
I	GLU189
I	GLU196
J	GLU134
J	GLU189
J	GLU196
K	GLU134
K	GLU189
K	GLU196
L	GLU134
L	GLU189
L	GLU196

site_id	SWS_FT_FI3
Number of Residues	48
Details	BINDING: BINDING => ECO:0000250\|UniProtKB:P9WN39

Chain	Residue	Details
D	GLU184
D	ASN240
D	ARG316
D	ARG321
E	GLU184
E	ASN240
E	ARG316
E	ARG321
F	GLU184
F	ASN240
F	ARG316
F	ARG321
G	GLU184
G	ASN240
G	ARG316
G	ARG321
H	GLU184
H	ASN240
H	ARG316
H	ARG321
I	GLU184
I	ASN240
I	ARG316
I	ARG321
J	GLU184
J	ASN240
J	ARG316
J	ARG321
K	GLU184
K	ASN240
K	ARG316
K	ARG321
L	GLU184
L	ASN240
L	ARG316
L	ARG321
A	GLU184
A	ASN240
A	ARG316
A	ARG321
B	GLU184
B	ASN240
B	ARG316
B	ARG321
C	GLU184
C	ASN240
C	ARG316
C	ARG321

site_id	SWS_FT_FI4
Number of Residues	12
Details	BINDING: BINDING => ECO:0000269\|PubMed:24158439, ECO:0000269\|PubMed:25691471, ECO:0007744\|PDB:4LNF, ECO:0007744\|PDB:4LNK, ECO:0007744\|PDB:4LNN, ECO:0007744\|PDB:4S0R

Chain	Residue	Details
B	GLY241
C	GLY241
D	GLY241
E	GLY241
F	GLY241
G	GLY241
H	GLY241
I	GLY241
J	GLY241
K	GLY241
L	GLY241
A	GLY241

site_id	SWS_FT_FI5
Number of Residues	12
Details	BINDING: BINDING => ECO:0000269\|PubMed:24158439, ECO:0000269\|PubMed:25691471, ECO:0007744\|PDB:4LNF, ECO:0007744\|PDB:4LNK, ECO:0007744\|PDB:4S0R

Chain	Residue	Details
A	HIS245
B	HIS245
C	HIS245
D	HIS245
E	HIS245
F	HIS245
G	HIS245
H	HIS245
I	HIS245
J	HIS245
K	HIS245
L	HIS245

site_id	SWS_FT_FI6
Number of Residues	12
Details	BINDING: BINDING => ECO:0000250\|UniProtKB:P77961

Chain	Residue	Details
A	SER249
B	SER249
C	SER249
D	SER249
E	SER249
F	SER249
G	SER249
H	SER249
I	SER249
J	SER249
K	SER249
L	SER249

site_id	SWS_FT_FI7
Number of Residues	36
Details	BINDING: BINDING => ECO:0000250\|UniProtKB:P0A1P6

Chain	Residue	Details
E	ARG298
E	GLU304
E	ARG335
F	ARG298
F	GLU304
F	ARG335
G	ARG298
G	GLU304
G	ARG335
H	ARG298
H	GLU304
H	ARG335
I	ARG298
I	GLU304
I	ARG335
J	ARG298
J	GLU304
J	ARG335
K	ARG298
K	GLU304
K	ARG335
L	ARG298
L	GLU304
L	ARG335
A	ARG298
A	GLU304
A	ARG335
B	ARG298
B	GLU304
B	ARG335
C	ARG298
C	GLU304
C	ARG335
D	ARG298
D	GLU304
D	ARG335

site_id	SWS_FT_FI8
Number of Residues	12
Details	SITE: Important for inhibition by glutamine => ECO:0000269\|PubMed:24158439

Chain	Residue	Details
A	ARG62
B	ARG62
C	ARG62
D	ARG62
E	ARG62
F	ARG62
G	ARG62
H	ARG62
I	ARG62
J	ARG62
K	ARG62
L	ARG62

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)