Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

4LNN

B. subtilis glutamine synthetase structures reveal large active site conformational changes and basis for isoenzyme specific regulation: structure of apo form of GS

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000166	molecular_function	nucleotide binding
A	0000287	molecular_function	magnesium ion binding
A	0003824	molecular_function	catalytic activity
A	0004356	molecular_function	glutamine synthetase activity
A	0005515	molecular_function	protein binding
A	0005524	molecular_function	ATP binding
A	0005737	cellular_component	cytoplasm
A	0006351	biological_process	DNA-templated transcription
A	0006542	biological_process	glutamine biosynthetic process
A	0016595	molecular_function	glutamate binding
A	0016874	molecular_function	ligase activity
A	0043562	biological_process	cellular response to nitrogen levels
A	0045892	biological_process	negative regulation of DNA-templated transcription
A	0046872	molecular_function	metal ion binding
A	0070406	molecular_function	glutamine binding
A	0090295	biological_process	nitrogen catabolite repression of transcription
A	0140297	molecular_function	DNA-binding transcription factor binding
A	0140416	molecular_function	transcription regulator inhibitor activity
B	0000166	molecular_function	nucleotide binding
B	0000287	molecular_function	magnesium ion binding
B	0003824	molecular_function	catalytic activity
B	0004356	molecular_function	glutamine synthetase activity
B	0005515	molecular_function	protein binding
B	0005524	molecular_function	ATP binding
B	0005737	cellular_component	cytoplasm
B	0006351	biological_process	DNA-templated transcription
B	0006542	biological_process	glutamine biosynthetic process
B	0016595	molecular_function	glutamate binding
B	0016874	molecular_function	ligase activity
B	0043562	biological_process	cellular response to nitrogen levels
B	0045892	biological_process	negative regulation of DNA-templated transcription
B	0046872	molecular_function	metal ion binding
B	0070406	molecular_function	glutamine binding
B	0090295	biological_process	nitrogen catabolite repression of transcription
B	0140297	molecular_function	DNA-binding transcription factor binding
B	0140416	molecular_function	transcription regulator inhibitor activity
C	0000166	molecular_function	nucleotide binding
C	0000287	molecular_function	magnesium ion binding
C	0003824	molecular_function	catalytic activity
C	0004356	molecular_function	glutamine synthetase activity
C	0005515	molecular_function	protein binding
C	0005524	molecular_function	ATP binding
C	0005737	cellular_component	cytoplasm
C	0006351	biological_process	DNA-templated transcription
C	0006542	biological_process	glutamine biosynthetic process
C	0016595	molecular_function	glutamate binding
C	0016874	molecular_function	ligase activity
C	0043562	biological_process	cellular response to nitrogen levels
C	0045892	biological_process	negative regulation of DNA-templated transcription
C	0046872	molecular_function	metal ion binding
C	0070406	molecular_function	glutamine binding
C	0090295	biological_process	nitrogen catabolite repression of transcription
C	0140297	molecular_function	DNA-binding transcription factor binding
C	0140416	molecular_function	transcription regulator inhibitor activity
D	0000166	molecular_function	nucleotide binding
D	0000287	molecular_function	magnesium ion binding
D	0003824	molecular_function	catalytic activity
D	0004356	molecular_function	glutamine synthetase activity
D	0005515	molecular_function	protein binding
D	0005524	molecular_function	ATP binding
D	0005737	cellular_component	cytoplasm
D	0006351	biological_process	DNA-templated transcription
D	0006542	biological_process	glutamine biosynthetic process
D	0016595	molecular_function	glutamate binding
D	0016874	molecular_function	ligase activity
D	0043562	biological_process	cellular response to nitrogen levels
D	0045892	biological_process	negative regulation of DNA-templated transcription
D	0046872	molecular_function	metal ion binding
D	0070406	molecular_function	glutamine binding
D	0090295	biological_process	nitrogen catabolite repression of transcription
D	0140297	molecular_function	DNA-binding transcription factor binding
D	0140416	molecular_function	transcription regulator inhibitor activity
E	0000166	molecular_function	nucleotide binding
E	0000287	molecular_function	magnesium ion binding
E	0003824	molecular_function	catalytic activity
E	0004356	molecular_function	glutamine synthetase activity
E	0005515	molecular_function	protein binding
E	0005524	molecular_function	ATP binding
E	0005737	cellular_component	cytoplasm
E	0006351	biological_process	DNA-templated transcription
E	0006542	biological_process	glutamine biosynthetic process
E	0016595	molecular_function	glutamate binding
E	0016874	molecular_function	ligase activity
E	0043562	biological_process	cellular response to nitrogen levels
E	0045892	biological_process	negative regulation of DNA-templated transcription
E	0046872	molecular_function	metal ion binding
E	0070406	molecular_function	glutamine binding
E	0090295	biological_process	nitrogen catabolite repression of transcription
E	0140297	molecular_function	DNA-binding transcription factor binding
E	0140416	molecular_function	transcription regulator inhibitor activity
F	0000166	molecular_function	nucleotide binding
F	0000287	molecular_function	magnesium ion binding
F	0003824	molecular_function	catalytic activity
F	0004356	molecular_function	glutamine synthetase activity
F	0005515	molecular_function	protein binding
F	0005524	molecular_function	ATP binding
F	0005737	cellular_component	cytoplasm
F	0006351	biological_process	DNA-templated transcription
F	0006542	biological_process	glutamine biosynthetic process
F	0016595	molecular_function	glutamate binding
F	0016874	molecular_function	ligase activity
F	0043562	biological_process	cellular response to nitrogen levels
F	0045892	biological_process	negative regulation of DNA-templated transcription
F	0046872	molecular_function	metal ion binding
F	0070406	molecular_function	glutamine binding
F	0090295	biological_process	nitrogen catabolite repression of transcription
F	0140297	molecular_function	DNA-binding transcription factor binding
F	0140416	molecular_function	transcription regulator inhibitor activity
G	0000166	molecular_function	nucleotide binding
G	0000287	molecular_function	magnesium ion binding
G	0003824	molecular_function	catalytic activity
G	0004356	molecular_function	glutamine synthetase activity
G	0005515	molecular_function	protein binding
G	0005524	molecular_function	ATP binding
G	0005737	cellular_component	cytoplasm
G	0006351	biological_process	DNA-templated transcription
G	0006542	biological_process	glutamine biosynthetic process
G	0016595	molecular_function	glutamate binding
G	0016874	molecular_function	ligase activity
G	0043562	biological_process	cellular response to nitrogen levels
G	0045892	biological_process	negative regulation of DNA-templated transcription
G	0046872	molecular_function	metal ion binding
G	0070406	molecular_function	glutamine binding
G	0090295	biological_process	nitrogen catabolite repression of transcription
G	0140297	molecular_function	DNA-binding transcription factor binding
G	0140416	molecular_function	transcription regulator inhibitor activity
H	0000166	molecular_function	nucleotide binding
H	0000287	molecular_function	magnesium ion binding
H	0003824	molecular_function	catalytic activity
H	0004356	molecular_function	glutamine synthetase activity
H	0005515	molecular_function	protein binding
H	0005524	molecular_function	ATP binding
H	0005737	cellular_component	cytoplasm
H	0006351	biological_process	DNA-templated transcription
H	0006542	biological_process	glutamine biosynthetic process
H	0016595	molecular_function	glutamate binding
H	0016874	molecular_function	ligase activity
H	0043562	biological_process	cellular response to nitrogen levels
H	0045892	biological_process	negative regulation of DNA-templated transcription
H	0046872	molecular_function	metal ion binding
H	0070406	molecular_function	glutamine binding
H	0090295	biological_process	nitrogen catabolite repression of transcription
H	0140297	molecular_function	DNA-binding transcription factor binding
H	0140416	molecular_function	transcription regulator inhibitor activity
I	0000166	molecular_function	nucleotide binding
I	0000287	molecular_function	magnesium ion binding
I	0003824	molecular_function	catalytic activity
I	0004356	molecular_function	glutamine synthetase activity
I	0005515	molecular_function	protein binding
I	0005524	molecular_function	ATP binding
I	0005737	cellular_component	cytoplasm
I	0006351	biological_process	DNA-templated transcription
I	0006542	biological_process	glutamine biosynthetic process
I	0016595	molecular_function	glutamate binding
I	0016874	molecular_function	ligase activity
I	0043562	biological_process	cellular response to nitrogen levels
I	0045892	biological_process	negative regulation of DNA-templated transcription
I	0046872	molecular_function	metal ion binding
I	0070406	molecular_function	glutamine binding
I	0090295	biological_process	nitrogen catabolite repression of transcription
I	0140297	molecular_function	DNA-binding transcription factor binding
I	0140416	molecular_function	transcription regulator inhibitor activity
J	0000166	molecular_function	nucleotide binding
J	0000287	molecular_function	magnesium ion binding
J	0003824	molecular_function	catalytic activity
J	0004356	molecular_function	glutamine synthetase activity
J	0005515	molecular_function	protein binding
J	0005524	molecular_function	ATP binding
J	0005737	cellular_component	cytoplasm
J	0006351	biological_process	DNA-templated transcription
J	0006542	biological_process	glutamine biosynthetic process
J	0016595	molecular_function	glutamate binding
J	0016874	molecular_function	ligase activity
J	0043562	biological_process	cellular response to nitrogen levels
J	0045892	biological_process	negative regulation of DNA-templated transcription
J	0046872	molecular_function	metal ion binding
J	0070406	molecular_function	glutamine binding
J	0090295	biological_process	nitrogen catabolite repression of transcription
J	0140297	molecular_function	DNA-binding transcription factor binding
J	0140416	molecular_function	transcription regulator inhibitor activity
K	0000166	molecular_function	nucleotide binding
K	0000287	molecular_function	magnesium ion binding
K	0003824	molecular_function	catalytic activity
K	0004356	molecular_function	glutamine synthetase activity
K	0005515	molecular_function	protein binding
K	0005524	molecular_function	ATP binding
K	0005737	cellular_component	cytoplasm
K	0006351	biological_process	DNA-templated transcription
K	0006542	biological_process	glutamine biosynthetic process
K	0016595	molecular_function	glutamate binding
K	0016874	molecular_function	ligase activity
K	0043562	biological_process	cellular response to nitrogen levels
K	0045892	biological_process	negative regulation of DNA-templated transcription
K	0046872	molecular_function	metal ion binding
K	0070406	molecular_function	glutamine binding
K	0090295	biological_process	nitrogen catabolite repression of transcription
K	0140297	molecular_function	DNA-binding transcription factor binding
K	0140416	molecular_function	transcription regulator inhibitor activity
L	0000166	molecular_function	nucleotide binding
L	0000287	molecular_function	magnesium ion binding
L	0003824	molecular_function	catalytic activity
L	0004356	molecular_function	glutamine synthetase activity
L	0005515	molecular_function	protein binding
L	0005524	molecular_function	ATP binding
L	0005737	cellular_component	cytoplasm
L	0006351	biological_process	DNA-templated transcription
L	0006542	biological_process	glutamine biosynthetic process
L	0016595	molecular_function	glutamate binding
L	0016874	molecular_function	ligase activity
L	0043562	biological_process	cellular response to nitrogen levels
L	0045892	biological_process	negative regulation of DNA-templated transcription
L	0046872	molecular_function	metal ion binding
L	0070406	molecular_function	glutamine binding
L	0090295	biological_process	nitrogen catabolite repression of transcription
L	0140297	molecular_function	DNA-binding transcription factor binding
L	0140416	molecular_function	transcription regulator inhibitor activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	3
Details	BINDING SITE FOR RESIDUE MG A 501

Chain	Residue
A	GLU134
A	GLU189
A	GLU196

site_id	AC2
Number of Residues	3
Details	BINDING SITE FOR RESIDUE MG A 502

Chain	Residue
A	GLU132
A	HIS245
A	GLU333

site_id	AC3
Number of Residues	4
Details	BINDING SITE FOR RESIDUE MG B 501

Chain	Residue
B	HOH610
B	GLU134
B	GLU189
B	GLU196

site_id	AC4
Number of Residues	2
Details	BINDING SITE FOR RESIDUE MG B 502

Chain	Residue
B	GLU132
B	GLU333

site_id	AC5
Number of Residues	2
Details	BINDING SITE FOR RESIDUE MG B 503

Chain	Residue
B	VAL61
B	HIS416

site_id	AC6
Number of Residues	3
Details	BINDING SITE FOR RESIDUE MG C 501

Chain	Residue
C	GLU134
C	GLU189
C	GLU196

site_id	AC7
Number of Residues	2
Details	BINDING SITE FOR RESIDUE MG C 502

Chain	Residue
C	GLU132
C	GLU333

site_id	AC8
Number of Residues	3
Details	BINDING SITE FOR RESIDUE SO4 D 501

Chain	Residue
D	VAL78
D	ARG206
D	ASP210

site_id	AC9
Number of Residues	3
Details	BINDING SITE FOR RESIDUE MG D 502

Chain	Residue
D	GLU134
D	GLU189
D	GLU196

site_id	BC1
Number of Residues	3
Details	BINDING SITE FOR RESIDUE MG D 503

Chain	Residue
D	GLU132
D	HIS245
D	GLU333

site_id	BC2
Number of Residues	3
Details	BINDING SITE FOR RESIDUE SO4 D 504

Chain	Residue
C	LYS224
D	GLU85
D	LYS86

site_id	BC3
Number of Residues	3
Details	BINDING SITE FOR RESIDUE MG E 501

Chain	Residue
E	GLU132
E	ARG321
E	GLU333

site_id	BC4
Number of Residues	3
Details	BINDING SITE FOR RESIDUE MG E 502

Chain	Residue
E	GLU134
E	GLU189
E	GLU196

site_id	BC5
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 E 503

Chain	Residue
E	THR83
E	ALA84
E	GLY87
E	LYS88

site_id	BC6
Number of Residues	5
Details	BINDING SITE FOR RESIDUE MG F 501

Chain	Residue
A	GLU65
F	GLU132
F	HIS245
F	ARG321
F	GLU333

site_id	BC7
Number of Residues	5
Details	BINDING SITE FOR RESIDUE MG F 502

Chain	Residue
F	GLU134
F	HIS187
F	GLU189
F	GLU196
F	HOH610

site_id	BC8
Number of Residues	3
Details	BINDING SITE FOR RESIDUE SO4 F 503

Chain	Residue
F	THR83
F	GLU85
F	LYS88

site_id	BC9
Number of Residues	3
Details	BINDING SITE FOR RESIDUE MG G 501

Chain	Residue
G	GLU134
G	GLU189
G	GLU196

site_id	CC1
Number of Residues	2
Details	BINDING SITE FOR RESIDUE MG G 502

Chain	Residue
G	GLU132
G	GLU333

site_id	CC2
Number of Residues	5
Details	BINDING SITE FOR RESIDUE SO4 H 501

Chain	Residue
H	THR83
H	ALA84
H	GLU85
H	GLY87
H	LYS88

site_id	CC3
Number of Residues	3
Details	BINDING SITE FOR RESIDUE MG H 502

Chain	Residue
H	GLU134
H	GLU189
H	GLU196

site_id	CC4
Number of Residues	2
Details	BINDING SITE FOR RESIDUE MG H 503

Chain	Residue
H	GLU132
H	GLU333

site_id	CC5
Number of Residues	3
Details	BINDING SITE FOR RESIDUE MG I 501

Chain	Residue
I	GLU134
I	GLU189
I	GLU196

site_id	CC6
Number of Residues	4
Details	BINDING SITE FOR RESIDUE MG I 502

Chain	Residue
H	GLU65
I	GLU132
I	ARG321
I	GLU333

site_id	CC7
Number of Residues	3
Details	BINDING SITE FOR RESIDUE MG J 501

Chain	Residue
J	GLU134
J	GLU189
J	GLU196

site_id	CC8
Number of Residues	2
Details	BINDING SITE FOR RESIDUE MG J 502

Chain	Residue
J	GLU132
J	GLU333

site_id	CC9
Number of Residues	3
Details	BINDING SITE FOR RESIDUE MG K 501

Chain	Residue
K	GLU134
K	GLU189
K	GLU196

site_id	DC1
Number of Residues	3
Details	BINDING SITE FOR RESIDUE MG K 502

Chain	Residue
K	GLU132
K	ARG321
K	GLU333

site_id	DC2
Number of Residues	3
Details	BINDING SITE FOR RESIDUE MG L 501

Chain	Residue
L	GLU134
L	GLU189
L	GLU196

site_id	DC3
Number of Residues	2
Details	BINDING SITE FOR RESIDUE MG L 502

Chain	Residue
L	GLU132
L	GLU333

Functional Information from PROSITE/UniProt

site_id	PS00180
Number of Residues	19
Details	GLNA_1 Glutamine synthetase signature 1. FDGSSiegfvrieESDmyL

Chain	Residue	Details
A	PHE52-LEU70

site_id	PS00181
Number of Residues	16
Details	GLNA_ATP Glutamine synthetase putative ATP-binding region signature. KPLfgv..NGSGmHcnlS

Chain	Residue	Details
A	LYS234-SER249

site_id	PS00182
Number of Residues	13
Details	GLNA_ADENYLATION Glutamine synthetase class-I adenylation site. KLeapapIDRNIY

Chain	Residue	Details
A	LYS361-TYR373

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1020
Details	Domain: {"description":"GS beta-grasp","evidences":[{"source":"PROSITE-ProRule","id":"PRU01330","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

site_id	SWS_FT_FI2
Number of Residues	24
Details	Binding site: {"evidences":[{"source":"PubMed","id":"24158439","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"25691471","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"4LNF","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4LNI","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4LNK","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4LNN","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4S0R","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI3
Number of Residues	36
Details	Binding site: {"evidences":[{"source":"PubMed","id":"24158439","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"25691471","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"4LNF","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4LNI","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4LNK","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4S0R","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI4
Number of Residues	48
Details	Binding site: {"evidences":[{"source":"UniProtKB","id":"P9WN39","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

site_id	SWS_FT_FI5
Number of Residues	12
Details	Binding site: {"evidences":[{"source":"PubMed","id":"24158439","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"25691471","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"4LNF","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4LNK","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4LNN","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4S0R","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI6
Number of Residues	12
Details	Binding site: {"evidences":[{"source":"PubMed","id":"24158439","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"25691471","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"4LNF","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4LNK","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4S0R","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI7
Number of Residues	12
Details	Binding site: {"evidences":[{"source":"UniProtKB","id":"P77961","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

site_id	SWS_FT_FI8
Number of Residues	24
Details	Binding site: {"evidences":[{"source":"UniProtKB","id":"P0A1P6","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

site_id	SWS_FT_FI9
Number of Residues	12
Details	Site: {"description":"Important for inhibition by glutamine","evidences":[{"source":"PubMed","id":"24158439","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)