4LNK
B. subtilis glutamine synthetase structures reveal large active site conformational changes and basis for isoenzyme specific regulation: structure of GS-glutamate-AMPPCP complex
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0000166 | molecular_function | nucleotide binding |
| A | 0000287 | molecular_function | magnesium ion binding |
| A | 0003824 | molecular_function | catalytic activity |
| A | 0004356 | molecular_function | glutamine synthetase activity |
| A | 0005515 | molecular_function | protein binding |
| A | 0005524 | molecular_function | ATP binding |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0006351 | biological_process | DNA-templated transcription |
| A | 0006542 | biological_process | glutamine biosynthetic process |
| A | 0016595 | molecular_function | glutamate binding |
| A | 0016874 | molecular_function | ligase activity |
| A | 0043562 | biological_process | cellular response to nitrogen levels |
| A | 0045892 | biological_process | negative regulation of DNA-templated transcription |
| A | 0046872 | molecular_function | metal ion binding |
| A | 0070406 | molecular_function | glutamine binding |
| A | 0090295 | biological_process | nitrogen catabolite repression of transcription |
| A | 0140297 | molecular_function | DNA-binding transcription factor binding |
| A | 0140416 | molecular_function | transcription regulator inhibitor activity |
| B | 0000166 | molecular_function | nucleotide binding |
| B | 0000287 | molecular_function | magnesium ion binding |
| B | 0003824 | molecular_function | catalytic activity |
| B | 0004356 | molecular_function | glutamine synthetase activity |
| B | 0005515 | molecular_function | protein binding |
| B | 0005524 | molecular_function | ATP binding |
| B | 0005737 | cellular_component | cytoplasm |
| B | 0006351 | biological_process | DNA-templated transcription |
| B | 0006542 | biological_process | glutamine biosynthetic process |
| B | 0016595 | molecular_function | glutamate binding |
| B | 0016874 | molecular_function | ligase activity |
| B | 0043562 | biological_process | cellular response to nitrogen levels |
| B | 0045892 | biological_process | negative regulation of DNA-templated transcription |
| B | 0046872 | molecular_function | metal ion binding |
| B | 0070406 | molecular_function | glutamine binding |
| B | 0090295 | biological_process | nitrogen catabolite repression of transcription |
| B | 0140297 | molecular_function | DNA-binding transcription factor binding |
| B | 0140416 | molecular_function | transcription regulator inhibitor activity |
| C | 0000166 | molecular_function | nucleotide binding |
| C | 0000287 | molecular_function | magnesium ion binding |
| C | 0003824 | molecular_function | catalytic activity |
| C | 0004356 | molecular_function | glutamine synthetase activity |
| C | 0005515 | molecular_function | protein binding |
| C | 0005524 | molecular_function | ATP binding |
| C | 0005737 | cellular_component | cytoplasm |
| C | 0006351 | biological_process | DNA-templated transcription |
| C | 0006542 | biological_process | glutamine biosynthetic process |
| C | 0016595 | molecular_function | glutamate binding |
| C | 0016874 | molecular_function | ligase activity |
| C | 0043562 | biological_process | cellular response to nitrogen levels |
| C | 0045892 | biological_process | negative regulation of DNA-templated transcription |
| C | 0046872 | molecular_function | metal ion binding |
| C | 0070406 | molecular_function | glutamine binding |
| C | 0090295 | biological_process | nitrogen catabolite repression of transcription |
| C | 0140297 | molecular_function | DNA-binding transcription factor binding |
| C | 0140416 | molecular_function | transcription regulator inhibitor activity |
| D | 0000166 | molecular_function | nucleotide binding |
| D | 0000287 | molecular_function | magnesium ion binding |
| D | 0003824 | molecular_function | catalytic activity |
| D | 0004356 | molecular_function | glutamine synthetase activity |
| D | 0005515 | molecular_function | protein binding |
| D | 0005524 | molecular_function | ATP binding |
| D | 0005737 | cellular_component | cytoplasm |
| D | 0006351 | biological_process | DNA-templated transcription |
| D | 0006542 | biological_process | glutamine biosynthetic process |
| D | 0016595 | molecular_function | glutamate binding |
| D | 0016874 | molecular_function | ligase activity |
| D | 0043562 | biological_process | cellular response to nitrogen levels |
| D | 0045892 | biological_process | negative regulation of DNA-templated transcription |
| D | 0046872 | molecular_function | metal ion binding |
| D | 0070406 | molecular_function | glutamine binding |
| D | 0090295 | biological_process | nitrogen catabolite repression of transcription |
| D | 0140297 | molecular_function | DNA-binding transcription factor binding |
| D | 0140416 | molecular_function | transcription regulator inhibitor activity |
| E | 0000166 | molecular_function | nucleotide binding |
| E | 0000287 | molecular_function | magnesium ion binding |
| E | 0003824 | molecular_function | catalytic activity |
| E | 0004356 | molecular_function | glutamine synthetase activity |
| E | 0005515 | molecular_function | protein binding |
| E | 0005524 | molecular_function | ATP binding |
| E | 0005737 | cellular_component | cytoplasm |
| E | 0006351 | biological_process | DNA-templated transcription |
| E | 0006542 | biological_process | glutamine biosynthetic process |
| E | 0016595 | molecular_function | glutamate binding |
| E | 0016874 | molecular_function | ligase activity |
| E | 0043562 | biological_process | cellular response to nitrogen levels |
| E | 0045892 | biological_process | negative regulation of DNA-templated transcription |
| E | 0046872 | molecular_function | metal ion binding |
| E | 0070406 | molecular_function | glutamine binding |
| E | 0090295 | biological_process | nitrogen catabolite repression of transcription |
| E | 0140297 | molecular_function | DNA-binding transcription factor binding |
| E | 0140416 | molecular_function | transcription regulator inhibitor activity |
| F | 0000166 | molecular_function | nucleotide binding |
| F | 0000287 | molecular_function | magnesium ion binding |
| F | 0003824 | molecular_function | catalytic activity |
| F | 0004356 | molecular_function | glutamine synthetase activity |
| F | 0005515 | molecular_function | protein binding |
| F | 0005524 | molecular_function | ATP binding |
| F | 0005737 | cellular_component | cytoplasm |
| F | 0006351 | biological_process | DNA-templated transcription |
| F | 0006542 | biological_process | glutamine biosynthetic process |
| F | 0016595 | molecular_function | glutamate binding |
| F | 0016874 | molecular_function | ligase activity |
| F | 0043562 | biological_process | cellular response to nitrogen levels |
| F | 0045892 | biological_process | negative regulation of DNA-templated transcription |
| F | 0046872 | molecular_function | metal ion binding |
| F | 0070406 | molecular_function | glutamine binding |
| F | 0090295 | biological_process | nitrogen catabolite repression of transcription |
| F | 0140297 | molecular_function | DNA-binding transcription factor binding |
| F | 0140416 | molecular_function | transcription regulator inhibitor activity |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 11 |
| Details | BINDING SITE FOR RESIDUE GLU A 501 |
| Chain | Residue |
| A | GLU134 |
| A | ARG335 |
| A | MG504 |
| A | GLU189 |
| A | VAL190 |
| A | ASN240 |
| A | GLY241 |
| A | GLY243 |
| A | HIS245 |
| A | ARG298 |
| A | GLU304 |
| site_id | AC2 |
| Number of Residues | 11 |
| Details | BINDING SITE FOR RESIDUE ADP A 502 |
| Chain | Residue |
| A | GLY130 |
| A | PHE199 |
| A | TYR201 |
| A | ASN247 |
| A | SER249 |
| A | SER325 |
| A | ILE328 |
| A | SER329 |
| A | ARG331 |
| B | LYS44 |
| B | MET51 |
| site_id | AC3 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE MG A 503 |
| Chain | Residue |
| A | GLU132 |
| A | HIS245 |
| A | GLU333 |
| B | GLU65 |
| site_id | AC4 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE MG A 504 |
| Chain | Residue |
| A | GLU134 |
| A | GLU189 |
| A | GLU196 |
| A | GLU501 |
| site_id | AC5 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE SO4 A 505 |
| Chain | Residue |
| A | ASN48 |
| A | LYS49 |
| A | TYR69 |
| A | TYR71 |
| site_id | AC6 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE SO4 A 506 |
| Chain | Residue |
| A | ASP73 |
| A | ASN75 |
| A | THR76 |
| A | ARG206 |
| A | ASP209 |
| A | HOH607 |
| site_id | AC7 |
| Number of Residues | 9 |
| Details | BINDING SITE FOR RESIDUE GLU B 501 |
| Chain | Residue |
| B | GLU134 |
| B | GLU189 |
| B | ASN240 |
| B | HIS245 |
| B | ARG298 |
| B | GLU304 |
| B | ARG335 |
| B | MG503 |
| C | ARG62 |
| site_id | AC8 |
| Number of Residues | 12 |
| Details | BINDING SITE FOR RESIDUE ADP B 502 |
| Chain | Residue |
| B | GLY130 |
| B | PHE199 |
| B | TYR201 |
| B | ASN247 |
| B | SER249 |
| B | SER325 |
| B | ILE328 |
| B | SER329 |
| B | THR330 |
| B | ARG331 |
| C | LYS44 |
| C | MET51 |
| site_id | AC9 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE MG B 503 |
| Chain | Residue |
| B | GLU134 |
| B | GLU189 |
| B | GLU196 |
| B | HIS245 |
| B | GLU501 |
| site_id | BC1 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE MG B 504 |
| Chain | Residue |
| B | GLU132 |
| B | HIS245 |
| B | GLU333 |
| C | GLU65 |
| site_id | BC2 |
| Number of Residues | 13 |
| Details | BINDING SITE FOR RESIDUE GLU C 501 |
| Chain | Residue |
| C | GLU134 |
| C | TYR156 |
| C | GLU189 |
| C | VAL190 |
| C | GLN194 |
| C | ASN240 |
| C | GLY241 |
| C | GLY243 |
| C | HIS245 |
| C | ARG298 |
| C | GLU304 |
| C | MG503 |
| C | HOH604 |
| site_id | BC3 |
| Number of Residues | 11 |
| Details | BINDING SITE FOR RESIDUE ADP C 502 |
| Chain | Residue |
| C | GLY130 |
| C | PHE199 |
| C | TYR201 |
| C | ASN247 |
| C | SER249 |
| C | SER325 |
| C | ILE328 |
| C | SER329 |
| C | ARG331 |
| D | LYS44 |
| D | MET51 |
| site_id | BC4 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE MG C 503 |
| Chain | Residue |
| C | GLU134 |
| C | GLU189 |
| C | GLU196 |
| C | GLU501 |
| site_id | BC5 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE MG C 504 |
| Chain | Residue |
| C | HIS245 |
| C | GLU333 |
| D | GLU65 |
| C | GLU132 |
| site_id | BC6 |
| Number of Residues | 12 |
| Details | BINDING SITE FOR RESIDUE GLU D 501 |
| Chain | Residue |
| D | GLU134 |
| D | TYR156 |
| D | GLU189 |
| D | ASN240 |
| D | GLY241 |
| D | GLY243 |
| D | HIS245 |
| D | ARG298 |
| D | TYR303 |
| D | GLU304 |
| D | ALA305 |
| D | MG503 |
| site_id | BC7 |
| Number of Residues | 12 |
| Details | BINDING SITE FOR RESIDUE ADP D 502 |
| Chain | Residue |
| D | GLY130 |
| D | GLU184 |
| D | PHE199 |
| D | TYR201 |
| D | ASN247 |
| D | SER249 |
| D | SER325 |
| D | ILE328 |
| D | SER329 |
| D | ARG331 |
| E | LYS44 |
| E | MET51 |
| site_id | BC8 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE MG D 503 |
| Chain | Residue |
| D | GLU134 |
| D | HIS187 |
| D | GLU189 |
| D | GLU196 |
| D | GLU501 |
| E | HOH601 |
| site_id | BC9 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE MG D 504 |
| Chain | Residue |
| D | GLU132 |
| D | HIS245 |
| D | GLU333 |
| E | GLU65 |
| E | HOH601 |
| site_id | CC1 |
| Number of Residues | 11 |
| Details | BINDING SITE FOR RESIDUE GLU E 501 |
| Chain | Residue |
| E | GLU134 |
| E | GLU189 |
| E | ASN240 |
| E | GLY241 |
| E | GLY243 |
| E | HIS245 |
| E | ARG298 |
| E | GLU304 |
| E | PRO306 |
| E | ARG335 |
| E | MG504 |
| site_id | CC2 |
| Number of Residues | 14 |
| Details | BINDING SITE FOR RESIDUE ADP E 502 |
| Chain | Residue |
| E | ASN128 |
| E | GLY130 |
| E | PHE199 |
| E | TYR201 |
| E | ASN247 |
| E | SER249 |
| E | PHE251 |
| E | SER325 |
| E | ILE328 |
| E | SER329 |
| E | ARG331 |
| F | LYS44 |
| F | LYS49 |
| F | MET51 |
| site_id | CC3 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE MG E 503 |
| Chain | Residue |
| E | GLU132 |
| E | HIS245 |
| E | GLU333 |
| F | GLU65 |
| site_id | CC4 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE MG E 504 |
| Chain | Residue |
| E | GLU132 |
| E | GLU134 |
| E | GLU189 |
| E | GLU196 |
| E | GLU501 |
| site_id | CC5 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE SO4 E 505 |
| Chain | Residue |
| E | ASN17 |
| E | THR83 |
| E | ALA84 |
| E | LYS86 |
| E | GLY87 |
| E | LYS88 |
| site_id | CC6 |
| Number of Residues | 9 |
| Details | BINDING SITE FOR RESIDUE GLU F 501 |
| Chain | Residue |
| A | ARG62 |
| F | GLU134 |
| F | GLU189 |
| F | VAL190 |
| F | ASN240 |
| F | GLY241 |
| F | HIS245 |
| F | ARG298 |
| F | GLU304 |
| site_id | CC7 |
| Number of Residues | 12 |
| Details | BINDING SITE FOR RESIDUE ADP F 502 |
| Chain | Residue |
| A | LYS44 |
| A | MET51 |
| F | GLY130 |
| F | GLU184 |
| F | PHE199 |
| F | TYR201 |
| F | ASN247 |
| F | SER249 |
| F | SER325 |
| F | ILE328 |
| F | SER329 |
| F | ARG331 |
| site_id | CC8 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE MG F 503 |
| Chain | Residue |
| F | GLU132 |
| F | HIS245 |
| F | GLU333 |
| site_id | CC9 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE MG F 504 |
| Chain | Residue |
| F | GLU134 |
| F | GLU189 |
| F | GLU196 |
Functional Information from PROSITE/UniProt
| site_id | PS00180 |
| Number of Residues | 19 |
| Details | GLNA_1 Glutamine synthetase signature 1. FDGSSiegfvrieESDmyL |
| Chain | Residue | Details |
| A | PHE52-LEU70 |
| site_id | PS00181 |
| Number of Residues | 16 |
| Details | GLNA_ATP Glutamine synthetase putative ATP-binding region signature. KPLfgv..NGSGmHcnlS |
| Chain | Residue | Details |
| A | LYS234-SER249 |
| site_id | PS00182 |
| Number of Residues | 13 |
| Details | GLNA_ADENYLATION Glutamine synthetase class-I adenylation site. KLeapapIDRNIY |
| Chain | Residue | Details |
| A | LYS361-TYR373 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 510 |
| Details | Domain: {"description":"GS beta-grasp","evidences":[{"source":"PROSITE-ProRule","id":"PRU01330","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 2016 |
| Details | Domain: {"description":"GS catalytic","evidences":[{"source":"PROSITE-ProRule","id":"PRU01331","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI3 |
| Number of Residues | 12 |
| Details | Binding site: {"evidences":[{"source":"PubMed","id":"24158439","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"25691471","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"4LNF","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4LNI","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4LNK","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4LNN","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4S0R","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI4 |
| Number of Residues | 18 |
| Details | Binding site: {"evidences":[{"source":"PubMed","id":"24158439","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"25691471","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"4LNF","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4LNI","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4LNK","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4S0R","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI5 |
| Number of Residues | 24 |
| Details | Binding site: {"evidences":[{"source":"UniProtKB","id":"P9WN39","evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI6 |
| Number of Residues | 6 |
| Details | Binding site: {"evidences":[{"source":"PubMed","id":"24158439","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"25691471","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"4LNF","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4LNK","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4LNN","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4S0R","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI7 |
| Number of Residues | 6 |
| Details | Binding site: {"evidences":[{"source":"PubMed","id":"24158439","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"25691471","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"4LNF","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4LNK","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4S0R","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI8 |
| Number of Residues | 6 |
| Details | Binding site: {"evidences":[{"source":"UniProtKB","id":"P77961","evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI9 |
| Number of Residues | 18 |
| Details | Binding site: {"evidences":[{"source":"UniProtKB","id":"P0A1P6","evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI10 |
| Number of Residues | 6 |
| Details | Site: {"description":"Important for inhibition by glutamine","evidences":[{"source":"PubMed","id":"24158439","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
