Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

4KFE

Crystal structure of Hansenula polymorpha copper amine oxidase-1 reduced by methylamine at pH 7.0

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0005507	molecular_function	copper ion binding
A	0005777	cellular_component	peroxisome
A	0008131	molecular_function	primary methylamine oxidase activity
A	0009308	biological_process	amine metabolic process
A	0016491	molecular_function	oxidoreductase activity
A	0016641	molecular_function	oxidoreductase activity, acting on the CH-NH2 group of donors, oxygen as acceptor
A	0046872	molecular_function	metal ion binding
A	0048038	molecular_function	quinone binding
B	0005507	molecular_function	copper ion binding
B	0005777	cellular_component	peroxisome
B	0008131	molecular_function	primary methylamine oxidase activity
B	0009308	biological_process	amine metabolic process
B	0016491	molecular_function	oxidoreductase activity
B	0016641	molecular_function	oxidoreductase activity, acting on the CH-NH2 group of donors, oxygen as acceptor
B	0046872	molecular_function	metal ion binding
B	0048038	molecular_function	quinone binding
C	0005507	molecular_function	copper ion binding
C	0005777	cellular_component	peroxisome
C	0008131	molecular_function	primary methylamine oxidase activity
C	0009308	biological_process	amine metabolic process
C	0016491	molecular_function	oxidoreductase activity
C	0016641	molecular_function	oxidoreductase activity, acting on the CH-NH2 group of donors, oxygen as acceptor
C	0046872	molecular_function	metal ion binding
C	0048038	molecular_function	quinone binding
D	0005507	molecular_function	copper ion binding
D	0005777	cellular_component	peroxisome
D	0008131	molecular_function	primary methylamine oxidase activity
D	0009308	biological_process	amine metabolic process
D	0016491	molecular_function	oxidoreductase activity
D	0016641	molecular_function	oxidoreductase activity, acting on the CH-NH2 group of donors, oxygen as acceptor
D	0046872	molecular_function	metal ion binding
D	0048038	molecular_function	quinone binding
E	0005507	molecular_function	copper ion binding
E	0005777	cellular_component	peroxisome
E	0008131	molecular_function	primary methylamine oxidase activity
E	0009308	biological_process	amine metabolic process
E	0016491	molecular_function	oxidoreductase activity
E	0016641	molecular_function	oxidoreductase activity, acting on the CH-NH2 group of donors, oxygen as acceptor
E	0046872	molecular_function	metal ion binding
E	0048038	molecular_function	quinone binding
F	0005507	molecular_function	copper ion binding
F	0005777	cellular_component	peroxisome
F	0008131	molecular_function	primary methylamine oxidase activity
F	0009308	biological_process	amine metabolic process
F	0016491	molecular_function	oxidoreductase activity
F	0016641	molecular_function	oxidoreductase activity, acting on the CH-NH2 group of donors, oxygen as acceptor
F	0046872	molecular_function	metal ion binding
F	0048038	molecular_function	quinone binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	4
Details	BINDING SITE FOR RESIDUE CU A 801

Chain	Residue
A	HIS456
A	HIS458
A	HIS624
A	PEO802

site_id	AC2
Number of Residues	8
Details	BINDING SITE FOR RESIDUE PEO A 802

Chain	Residue
A	MET634
A	CU801
A	HOH1055
A	TYY405
A	LEU429
A	HIS456
A	HIS458
A	HIS624

site_id	AC3
Number of Residues	4
Details	BINDING SITE FOR RESIDUE FOR A 803

Chain	Residue
A	TRP156
A	ASP319
A	TYR323
A	TYY405

site_id	AC4
Number of Residues	7
Details	BINDING SITE FOR RESIDUE GOL A 804

Chain	Residue
A	GLN66
A	GLN70
A	GLY72
A	HOH955
A	HOH1353
F	TYR534
F	GLU651

site_id	AC5
Number of Residues	6
Details	BINDING SITE FOR RESIDUE GOL A 805

Chain	Residue
A	LYS214
A	VAL215
A	GLY435
A	ASP436
A	ASN450
A	HOH1133

site_id	AC6
Number of Residues	8
Details	BINDING SITE FOR RESIDUE GOL A 806

Chain	Residue
A	GLU368
A	LYS393
A	TYR410
A	ASP422
A	ARG424
A	HOH1072
A	HOH1542
B	GLY371

site_id	AC7
Number of Residues	3
Details	BINDING SITE FOR RESIDUE GOL A 807

Chain	Residue
A	PRO484
A	TYR485
A	HOH1636

site_id	AC8
Number of Residues	4
Details	BINDING SITE FOR RESIDUE GOL A 808

Chain	Residue
A	HIS218
A	LYS219
A	TYR448
A	HOH1185

site_id	AC9
Number of Residues	8
Details	BINDING SITE FOR RESIDUE GOL A 809

Chain	Residue
A	TYR160
A	LYS561
A	SER591
A	HOH1246
F	LYS561
F	ASP593
F	GOL811
F	HOH994

site_id	BC1
Number of Residues	6
Details	BINDING SITE FOR RESIDUE GOL A 810

Chain	Residue
A	GLU110
A	HOH1149
A	HOH1509
F	ALA133
F	ILE204
F	ILE206

site_id	BC2
Number of Residues	2
Details	BINDING SITE FOR RESIDUE GOL A 811

Chain	Residue
A	LYS68
F	HOH1601

site_id	BC3
Number of Residues	6
Details	BINDING SITE FOR RESIDUE GOL A 812

Chain	Residue
A	GLY142
A	PRO144
A	GLU147
A	TYR177
A	HOH1624
E	HOH1357

site_id	BC4
Number of Residues	6
Details	BINDING SITE FOR RESIDUE GOL A 813

Chain	Residue
A	ARG61
A	ILE65
A	ASP471
A	ASP613
A	HOH1327
A	HOH1610

site_id	BC5
Number of Residues	7
Details	BINDING SITE FOR RESIDUE GOL A 814

Chain	Residue
A	ASP131
A	ALA133
A	VAL203
A	ILE204
A	HOH1645
F	GLU110
F	HOH1432

site_id	BC6
Number of Residues	9
Details	BINDING SITE FOR RESIDUE GOL A 815

Chain	Residue
A	PRO59
A	ARG61
A	HIS294
A	TYR575
A	ASN578
A	HOH911
A	HOH1141
A	HOH1305
A	HOH1406

site_id	BC7
Number of Residues	7
Details	BINDING SITE FOR RESIDUE GOL A 816

Chain	Residue
A	SER251
A	LYS253
A	GLU260
A	TRP261
A	SER262
A	HOH1192
A	HOH1257

site_id	BC8
Number of Residues	4
Details	BINDING SITE FOR RESIDUE CU B 801

Chain	Residue
B	HIS456
B	HIS458
B	HIS624
B	PEO802

site_id	BC9
Number of Residues	8
Details	BINDING SITE FOR RESIDUE PEO B 802

Chain	Residue
B	TYY405
B	LEU429
B	HIS456
B	HIS458
B	HIS624
B	MET634
B	CU801
B	HOH1094

site_id	CC1
Number of Residues	3
Details	BINDING SITE FOR RESIDUE FOR B 803

Chain	Residue
B	TRP156
B	TYR323
B	TYY405

site_id	CC2
Number of Residues	8
Details	BINDING SITE FOR RESIDUE GOL B 804

Chain	Residue
A	GLY371
B	GLU368
B	LYS393
B	VAL412
B	ASP422
B	HOH1028
B	HOH1472
B	HOH1496

site_id	CC3
Number of Residues	9
Details	BINDING SITE FOR RESIDUE GOL B 805

Chain	Residue
B	GLU58
B	PRO59
B	HIS294
B	TYR575
B	ASN578
B	HOH907
B	HOH1037
B	HOH1049
B	HOH1285

site_id	CC4
Number of Residues	3
Details	BINDING SITE FOR RESIDUE GOL B 806

Chain	Residue
B	PRO484
B	TYR499
B	HOH1630

site_id	CC5
Number of Residues	7
Details	BINDING SITE FOR RESIDUE GOL B 807

Chain	Residue
A	TYR534
B	HIS218
B	LYS219
B	TYR448
B	PRO449
B	PO4812
B	HOH1593

site_id	CC6
Number of Residues	8
Details	BINDING SITE FOR RESIDUE GOL B 808

Chain	Residue
B	LEU558
B	LYS561
B	SER591
B	GOL809
B	HOH988
B	HOH1394
C	LYS561
C	ASP593

site_id	CC7
Number of Residues	9
Details	BINDING SITE FOR RESIDUE GOL B 809

Chain	Residue
B	LYS561
B	ASP593
B	GOL808
B	HOH988
B	HOH1487
C	TYR160
C	LYS561
C	SER591
C	HOH1512

site_id	CC8
Number of Residues	5
Details	BINDING SITE FOR RESIDUE GOL B 810

Chain	Residue
B	ARG61
B	ILE65
B	ASP471
B	ASP613
B	HOH1592

site_id	CC9
Number of Residues	7
Details	BINDING SITE FOR RESIDUE GOL B 811

Chain	Residue
B	ARG213
B	LYS214
B	VAL215
B	ASP436
B	ASN450
B	HOH1021
B	HOH1617

site_id	DC1
Number of Residues	3
Details	BINDING SITE FOR RESIDUE PO4 B 812

Chain	Residue
B	HIS218
B	LYS219
B	GOL807

site_id	DC2
Number of Residues	5
Details	BINDING SITE FOR RESIDUE GOL B 813

Chain	Residue
B	PRO144
B	GLU147
B	TYR177
B	HOH1146
D	HOH1410

site_id	DC3
Number of Residues	5
Details	BINDING SITE FOR RESIDUE CU C 801

Chain	Residue
C	HIS456
C	HIS458
C	HIS624
C	PEO802
C	HOH1426

site_id	DC4
Number of Residues	8
Details	BINDING SITE FOR RESIDUE PEO C 802

Chain	Residue
C	TYY405
C	HIS456
C	HIS458
C	HIS624
C	MET634
C	CU801
C	HOH990
C	HOH1426

site_id	DC5
Number of Residues	6
Details	BINDING SITE FOR RESIDUE GOL C 803

Chain	Residue
C	HIS23
C	TYR64
C	LYS68
C	ASP280
C	HOH964
C	HOH1521

site_id	DC6
Number of Residues	5
Details	BINDING SITE FOR RESIDUE GOL C 804

Chain	Residue
C	LYS214
C	VAL215
C	ASP436
C	HOH1116
C	HOH1256

site_id	DC7
Number of Residues	1
Details	BINDING SITE FOR RESIDUE GOL C 805

Chain	Residue
B	LYS68

site_id	DC8
Number of Residues	5
Details	BINDING SITE FOR RESIDUE GOL C 806

Chain	Residue
C	LYS393
C	VAL412
C	ASP422
C	HOH1033
C	HOH1562

site_id	DC9
Number of Residues	2
Details	BINDING SITE FOR RESIDUE GOL C 807

Chain	Residue
C	TYR499
D	PRO442

site_id	EC1
Number of Residues	2
Details	BINDING SITE FOR RESIDUE PO4 C 808

Chain	Residue
C	HIS218
C	LYS219

site_id	EC2
Number of Residues	8
Details	BINDING SITE FOR RESIDUE GOL C 809

Chain	Residue
B	GLN66
B	GLN70
B	GLY72
C	TYR534
C	GLU651
C	HOH942
C	HOH1487
D	LYS231

site_id	EC3
Number of Residues	9
Details	BINDING SITE FOR RESIDUE GOL C 810

Chain	Residue
C	ARG57
C	TYR80
C	ASN578
C	TYR581
C	ASP585
C	HOH1146
C	HOH1262
C	HOH1363
C	HOH1495

site_id	EC4
Number of Residues	8
Details	BINDING SITE FOR RESIDUE PEO D 701

Chain	Residue
D	TYY405
D	LEU429
D	HIS456
D	HIS458
D	HIS624
D	MET634
D	CU703
D	HOH805

site_id	EC5
Number of Residues	4
Details	BINDING SITE FOR RESIDUE FOR D 702

Chain	Residue
D	TRP156
D	ASP319
D	TYR323
D	TYY405

site_id	EC6
Number of Residues	5
Details	BINDING SITE FOR RESIDUE CU D 703

Chain	Residue
D	HIS456
D	HIS458
D	HIS624
D	PEO701
D	HOH1351

site_id	EC7
Number of Residues	7
Details	BINDING SITE FOR RESIDUE GOL D 704

Chain	Residue
D	GLY142
D	PRO144
D	GLU147
D	TYR177
D	LYS217
D	HOH1127
D	HOH1466

site_id	EC8
Number of Residues	1
Details	BINDING SITE FOR RESIDUE GOL D 705

Chain	Residue
D	LYS150

site_id	EC9
Number of Residues	1
Details	BINDING SITE FOR RESIDUE GOL D 706

Chain	Residue
D	TYR499

site_id	FC1
Number of Residues	10
Details	BINDING SITE FOR RESIDUE GOL D 707

Chain	Residue
B	LYS62
B	ALA63
B	GLN66
B	PRO73
B	HOH1007
B	HOH1345
B	HOH1581
D	LYS231
D	VAL232
D	GLY233

site_id	FC2
Number of Residues	7
Details	BINDING SITE FOR RESIDUE GOL D 708

Chain	Residue
D	GLU368
D	LYS393
D	VAL412
D	ASP422
D	ARG424
D	HOH998
D	HOH1429

site_id	FC3
Number of Residues	6
Details	BINDING SITE FOR RESIDUE GOL D 709

Chain	Residue
D	HIS23
D	TYR64
D	LYS68
D	LYS265
D	ASP280
D	HOH909

site_id	FC4
Number of Residues	2
Details	BINDING SITE FOR RESIDUE GOL D 710

Chain	Residue
D	TRP67
D	LYS68

site_id	FC5
Number of Residues	7
Details	BINDING SITE FOR RESIDUE GOL D 711

Chain	Residue
D	LEU56
D	ARG57
D	TYR80
D	LEU580
D	HOH993
D	HOH1089
D	HOH1487

site_id	FC6
Number of Residues	5
Details	BINDING SITE FOR RESIDUE GOL D 712

Chain	Residue
D	PHE252
D	LYS253
D	MET254
D	ILE342
D	HIS343

site_id	FC7
Number of Residues	5
Details	BINDING SITE FOR RESIDUE CU E 801

Chain	Residue
E	HIS456
E	HIS458
E	HIS624
E	PEO802
E	HOH1432

site_id	FC8
Number of Residues	8
Details	BINDING SITE FOR RESIDUE PEO E 802

Chain	Residue
E	TYY405
E	LEU429
E	HIS456
E	HIS458
E	HIS624
E	MET634
E	CU801
E	HOH903

site_id	FC9
Number of Residues	6
Details	BINDING SITE FOR RESIDUE GOL E 803

Chain	Residue
E	GLY142
E	PRO144
E	GLU147
E	TYR177
E	LYS217
E	HOH1248

site_id	GC1
Number of Residues	6
Details	BINDING SITE FOR RESIDUE GOL E 804

Chain	Residue
E	PHE252
E	LYS253
E	MET254
E	ILE342
E	HIS343
E	HOH1486

site_id	GC2
Number of Residues	10
Details	BINDING SITE FOR RESIDUE GOL E 805

Chain	Residue
E	GLU58
E	PRO59
E	ARG295
E	TYR575
E	ASP577
E	ASN578
E	HOH948
E	HOH957
E	HOH1477
E	HOH1502

site_id	GC3
Number of Residues	5
Details	BINDING SITE FOR RESIDUE GOL E 806

Chain	Residue
E	HIS23
E	TYR64
E	LYS68
E	LYS265
E	ASP280

site_id	GC4
Number of Residues	9
Details	BINDING SITE FOR RESIDUE GOL E 807

Chain	Residue
E	GLU368
E	LYS393
E	ARG420
E	ASP422
E	ARG424
E	HOH1036
E	HOH1308
E	HOH1441
F	GLY371

site_id	GC5
Number of Residues	5
Details	BINDING SITE FOR RESIDUE CU F 801

Chain	Residue
F	HIS456
F	HIS458
F	HIS624
F	PEO802
F	HOH1395

site_id	GC6
Number of Residues	8
Details	BINDING SITE FOR RESIDUE PEO F 802

Chain	Residue
F	TYY405
F	HIS456
F	HIS458
F	HIS624
F	MET634
F	CU801
F	HOH983
F	HOH1395

site_id	GC7
Number of Residues	6
Details	BINDING SITE FOR RESIDUE GOL F 803

Chain	Residue
F	HIS23
F	TYR64
F	LYS68
F	LYS265
F	ASP280
F	HOH946

site_id	GC8
Number of Residues	8
Details	BINDING SITE FOR RESIDUE GOL F 804

Chain	Residue
E	GLY371
F	LYS393
F	VAL412
F	ASP422
F	ARG424
F	GOL805
F	HOH1036
F	HOH1525

site_id	GC9
Number of Residues	8
Details	BINDING SITE FOR RESIDUE GOL F 805

Chain	Residue
E	GLY371
E	ARG390
F	CYS338
F	LYS339
F	HIS366
F	GLU368
F	GOL804
F	HOH1334

site_id	HC1
Number of Residues	2
Details	BINDING SITE FOR RESIDUE GOL F 806

Chain	Residue
E	PRO484
F	PRO442

site_id	HC2
Number of Residues	5
Details	BINDING SITE FOR RESIDUE GOL F 807

Chain	Residue
F	PHE252
F	LYS253
F	MET254
F	HIS343
F	HOH1203

site_id	HC3
Number of Residues	1
Details	BINDING SITE FOR RESIDUE GOL F 808

Chain	Residue
F	PRO484

site_id	HC4
Number of Residues	3
Details	BINDING SITE FOR RESIDUE PO4 F 809

Chain	Residue
F	HIS218
F	LYS219
F	HOH1528

site_id	HC5
Number of Residues	6
Details	BINDING SITE FOR RESIDUE GOL F 810

Chain	Residue
F	LEU512
F	ASP604
F	GLY605
F	HOH1069
F	HOH1375
F	HOH1596

site_id	HC6
Number of Residues	9
Details	BINDING SITE FOR RESIDUE GOL F 811

Chain	Residue
A	LYS561
A	ASP593
A	GOL809
F	TYR160
F	LEU558
F	LYS561
F	SER591
F	HOH994
F	HOH1489

site_id	HC7
Number of Residues	7
Details	BINDING SITE FOR RESIDUE GOL F 812

Chain	Residue
F	GLU58
F	PRO59
F	TYR575
F	ASN578
F	HOH924
F	HOH987
F	HOH1182

Functional Information from PROSITE/UniProt

site_id	PS01159
Number of Residues	26
Details	WW_DOMAIN_1 WW/rsp5/WWP domain signature. WgtgkrlqqalvYYrsdedd.SQYSHP

Chain	Residue	Details
A	TRP164-PRO189

site_id	PS01164
Number of Residues	14
Details	COPPER_AMINE_OXID_1 Copper amine oxidase topaquinone signature. LVVsqifTaaNYEY

Chain	Residue	Details
A	LEU394-TYR407

site_id	PS01165
Number of Residues	14
Details	COPPER_AMINE_OXID_2 Copper amine oxidase copper-binding site signature. TfGitHFpapEDfP

Chain	Residue	Details
A	THR619-PRO632

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	6
Details	Active site: {"description":"Proton acceptor","evidences":[{"source":"PubMed","id":"9551552","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI2
Number of Residues	6
Details	Active site: {"description":"Schiff-base intermediate with substrate; via topaquinone","evidences":[{"source":"PubMed","id":"10933787","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"9551552","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1EKM","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3NBB","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3NBJ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3T0U","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4KFF","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI3
Number of Residues	66
Details	Binding site: {"evidences":[{"source":"PDB","id":"4EV2","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4EV5","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI4
Number of Residues	18
Details	Binding site: {"evidences":[{"source":"PubMed","id":"10933787","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"9551552","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1A2V","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1EKM","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2OOV","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2OQE","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3N9H","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3NBB","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3NBJ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3T0U","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4EV2","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4EV5","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4KFD","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4KFE","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4KFF","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI5
Number of Residues	18
Details	Binding site: {"evidences":[{"source":"UniProtKB","id":"Q43077","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

site_id	SWS_FT_FI6
Number of Residues	6
Details	Modified residue: {"description":"2',4',5'-topaquinone","evidences":[{"source":"PubMed","id":"9551552","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1A2V","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2OQE","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI7
Number of Residues	6
Details	Glycosylation: {"description":"N-linked (GlcNAc...) asparagine","evidences":[{"source":"PubMed","id":"9551552","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)