4KFD

Crystal structure of Hansenula polymorpha copper amine oxidase-1 reduced by methylamine at pH 6.0

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0005507	molecular_function	copper ion binding
A	0005777	cellular_component	peroxisome
A	0008131	molecular_function	primary methylamine oxidase activity
A	0009308	biological_process	amine metabolic process
A	0016491	molecular_function	oxidoreductase activity
A	0016641	molecular_function	oxidoreductase activity, acting on the CH-NH2 group of donors, oxygen as acceptor
A	0046872	molecular_function	metal ion binding
A	0048038	molecular_function	quinone binding
B	0005507	molecular_function	copper ion binding
B	0005777	cellular_component	peroxisome
B	0008131	molecular_function	primary methylamine oxidase activity
B	0009308	biological_process	amine metabolic process
B	0016491	molecular_function	oxidoreductase activity
B	0016641	molecular_function	oxidoreductase activity, acting on the CH-NH2 group of donors, oxygen as acceptor
B	0046872	molecular_function	metal ion binding
B	0048038	molecular_function	quinone binding
C	0005507	molecular_function	copper ion binding
C	0005777	cellular_component	peroxisome
C	0008131	molecular_function	primary methylamine oxidase activity
C	0009308	biological_process	amine metabolic process
C	0016491	molecular_function	oxidoreductase activity
C	0016641	molecular_function	oxidoreductase activity, acting on the CH-NH2 group of donors, oxygen as acceptor
C	0046872	molecular_function	metal ion binding
C	0048038	molecular_function	quinone binding
D	0005507	molecular_function	copper ion binding
D	0005777	cellular_component	peroxisome
D	0008131	molecular_function	primary methylamine oxidase activity
D	0009308	biological_process	amine metabolic process
D	0016491	molecular_function	oxidoreductase activity
D	0016641	molecular_function	oxidoreductase activity, acting on the CH-NH2 group of donors, oxygen as acceptor
D	0046872	molecular_function	metal ion binding
D	0048038	molecular_function	quinone binding
E	0005507	molecular_function	copper ion binding
E	0005777	cellular_component	peroxisome
E	0008131	molecular_function	primary methylamine oxidase activity
E	0009308	biological_process	amine metabolic process
E	0016491	molecular_function	oxidoreductase activity
E	0016641	molecular_function	oxidoreductase activity, acting on the CH-NH2 group of donors, oxygen as acceptor
E	0046872	molecular_function	metal ion binding
E	0048038	molecular_function	quinone binding
F	0005507	molecular_function	copper ion binding
F	0005777	cellular_component	peroxisome
F	0008131	molecular_function	primary methylamine oxidase activity
F	0009308	biological_process	amine metabolic process
F	0016491	molecular_function	oxidoreductase activity
F	0016641	molecular_function	oxidoreductase activity, acting on the CH-NH2 group of donors, oxygen as acceptor
F	0046872	molecular_function	metal ion binding
F	0048038	molecular_function	quinone binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	5
Details	BINDING SITE FOR RESIDUE CU A 801

Chain	Residue
A	HIS456
A	HIS458
A	HIS624
A	PEO802
A	HOH1428

---

site_id	AC2
Number of Residues	7
Details	BINDING SITE FOR RESIDUE PEO A 802

Chain	Residue
A	CU801
A	HOH1052
A	HOH1428
A	TYY405
A	HIS456
A	HIS624
A	MET634

---

site_id	AC3
Number of Residues	7
Details	BINDING SITE FOR RESIDUE GOL A 803

Chain	Residue
A	GLN66
A	GLN70
A	GLY72
A	HOH954
A	HOH1348
F	TYR534
F	THR650

---

site_id	AC4
Number of Residues	5
Details	BINDING SITE FOR RESIDUE GOL A 804

Chain	Residue
A	LYS214
A	VAL215
A	GLY435
A	ASP436
A	HOH1131

---

site_id	AC5
Number of Residues	8
Details	BINDING SITE FOR RESIDUE GOL A 805

Chain	Residue
A	GLU368
A	LYS393
A	VAL412
A	ASP422
A	HOH1069
A	HOH1561
A	HOH1579
B	GLY371

---

site_id	AC6
Number of Residues	3
Details	BINDING SITE FOR RESIDUE GOL A 806

Chain	Residue
A	PRO484
A	HOH1690
B	PRO442

---

site_id	AC7
Number of Residues	3
Details	BINDING SITE FOR RESIDUE GOL A 807

Chain	Residue
A	HIS218
A	LYS219
A	TYR448

---

site_id	AC8
Number of Residues	7
Details	BINDING SITE FOR RESIDUE GOL A 808

Chain	Residue
A	LYS561
A	SER591
A	HOH1245
F	LYS561
F	ASP593
F	GOL811
F	HOH996

---

site_id	AC9
Number of Residues	3
Details	BINDING SITE FOR RESIDUE GOL A 809

Chain	Residue
A	TRP67
A	LYS68
F	HOH1636

---

site_id	BC1
Number of Residues	6
Details	BINDING SITE FOR RESIDUE GOL A 810

Chain	Residue
A	GLY142
A	PRO144
A	GLU147
A	TYR177
A	HOH1678
E	HOH1355

---

site_id	BC2
Number of Residues	8
Details	BINDING SITE FOR RESIDUE GOL A 811

Chain	Residue
A	ARG61
A	LYS62
A	ILE65
A	ASP471
A	ASP613
A	HOH1323
A	HOH1533
A	HOH1663

---

site_id	BC3
Number of Residues	6
Details	BINDING SITE FOR RESIDUE GOL A 812

Chain	Residue
A	ASP131
A	VAL203
A	ILE204
A	HOH1495
A	HOH1698
F	GLU110

---

site_id	BC4
Number of Residues	9
Details	BINDING SITE FOR RESIDUE GOL A 813

Chain	Residue
A	PRO59
A	ARG61
A	HIS294
A	TYR575
A	ASN578
A	HOH911
A	HOH1139
A	HOH1302
A	HOH1407

---

site_id	BC5
Number of Residues	5
Details	BINDING SITE FOR RESIDUE GOL A 814

Chain	Residue
A	SER251
A	LYS253
A	GLU260
A	HOH1191
A	HOH1255

---

site_id	BC6
Number of Residues	4
Details	BINDING SITE FOR RESIDUE CU B 801

Chain	Residue
B	HIS456
B	HIS458
B	HIS624
B	PEO802

---

site_id	BC7
Number of Residues	8
Details	BINDING SITE FOR RESIDUE PEO B 802

Chain	Residue
B	TYY405
B	LEU429
B	HIS456
B	HIS458
B	HIS624
B	MET634
B	CU801
B	HOH1096

---

site_id	BC8
Number of Residues	8
Details	BINDING SITE FOR RESIDUE GOL B 803

Chain	Residue
B	ASP422
B	HOH1031
B	HOH1527
A	GLY371
B	GLU368
B	LYS393
B	VAL412
B	ARG420

---

site_id	BC9
Number of Residues	10
Details	BINDING SITE FOR RESIDUE GOL B 804

Chain	Residue
B	PRO59
B	ALA60
B	ARG61
B	HIS294
B	TYR575
B	ASN578
B	HOH908
B	HOH1039
B	HOH1051
B	HOH1287

---

site_id	CC1
Number of Residues	3
Details	BINDING SITE FOR RESIDUE GOL B 805

Chain	Residue
A	PRO442
B	PRO484
B	TYR499

---

site_id	CC2
Number of Residues	6
Details	BINDING SITE FOR RESIDUE GOL B 806

Chain	Residue
A	TYR534
B	HIS218
B	LYS219
B	TYR448
B	PO4810
B	HOH1636

---

site_id	CC3
Number of Residues	7
Details	BINDING SITE FOR RESIDUE GOL B 807

Chain	Residue
B	LYS561
B	SER591
B	HOH990
B	HOH1399
C	LYS561
C	ASP593
C	GOL811

---

site_id	CC4
Number of Residues	5
Details	BINDING SITE FOR RESIDUE GOL B 808

Chain	Residue
B	ARG61
B	ASP613
B	HOH1516
B	HOH1635
D	HOH1396

---

site_id	CC5
Number of Residues	6
Details	BINDING SITE FOR RESIDUE GOL B 809

Chain	Residue
B	LYS214
B	VAL215
B	ASP436
B	ASN450
B	HOH1023
B	HOH1659

---

site_id	CC6
Number of Residues	3
Details	BINDING SITE FOR RESIDUE PO4 B 810

Chain	Residue
B	HIS218
B	LYS219
B	GOL806

---

site_id	CC7
Number of Residues	7
Details	BINDING SITE FOR RESIDUE GOL B 811

Chain	Residue
B	GLY142
B	PRO144
B	GLU147
B	TYR177
B	VAL215
B	HOH1147
D	HOH1545

---

site_id	CC8
Number of Residues	5
Details	BINDING SITE FOR RESIDUE CU C 801

Chain	Residue
C	HIS456
C	HIS458
C	HIS624
C	PEO802
C	HOH1427

---

site_id	CC9
Number of Residues	8
Details	BINDING SITE FOR RESIDUE PEO C 802

Chain	Residue
C	TYY405
C	HIS456
C	HIS458
C	HIS624
C	MET634
C	CU801
C	HOH990
C	HOH1427

---

site_id	DC1
Number of Residues	7
Details	BINDING SITE FOR RESIDUE GOL C 803

Chain	Residue
C	HIS23
C	TYR64
C	LYS68
C	LYS265
C	ASP280
C	HOH965
C	HOH1543

---

site_id	DC2
Number of Residues	5
Details	BINDING SITE FOR RESIDUE GOL C 804

Chain	Residue
C	LYS214
C	VAL215
C	GLY435
C	ASP436
C	HOH1252

---

site_id	DC3
Number of Residues	2
Details	BINDING SITE FOR RESIDUE GOL C 805

Chain	Residue
B	LYS68
C	HOH1556

---

site_id	DC4
Number of Residues	8
Details	BINDING SITE FOR RESIDUE GOL C 806

Chain	Residue
C	GLU368
C	LYS393
C	VAL412
C	ARG420
C	ASP422
C	HOH1034
C	HOH1590
D	GLY371

---

site_id	DC5
Number of Residues	3
Details	BINDING SITE FOR RESIDUE GOL C 807

Chain	Residue
C	PRO484
C	TYR499
D	PRO442

---

site_id	DC6
Number of Residues	2
Details	BINDING SITE FOR RESIDUE PO4 C 808

Chain	Residue
C	HIS218
C	LYS219

---

site_id	DC7
Number of Residues	9
Details	BINDING SITE FOR RESIDUE GOL C 809

Chain	Residue
B	GLN66
B	GLN70
B	GLY72
C	TYR534
C	THR650
C	GLU651
C	HOH942
C	HOH1509
D	LYS231

---

site_id	DC8
Number of Residues	10
Details	BINDING SITE FOR RESIDUE GOL C 810

Chain	Residue
C	ARG57
C	TYR80
C	ASN578
C	TYR581
C	ASP585
C	HOH1146
C	HOH1257
C	HOH1361
C	HOH1412
C	HOH1516

---

site_id	DC9
Number of Residues	8
Details	BINDING SITE FOR RESIDUE GOL C 811

Chain	Residue
B	LYS561
B	ASP593
B	GOL807
B	HOH990
C	TYR160
C	LYS561
C	SER591
C	HOH1535

---

site_id	EC1
Number of Residues	5
Details	BINDING SITE FOR RESIDUE CU D 801

Chain	Residue
D	HIS456
D	HIS458
D	HIS624
D	PEO802
D	HOH1473

---

site_id	EC2
Number of Residues	7
Details	BINDING SITE FOR RESIDUE PEO D 802

Chain	Residue
D	TYY405
D	HIS456
D	HIS458
D	HIS624
D	MET634
D	CU801
D	HOH905

---

site_id	EC3
Number of Residues	8
Details	BINDING SITE FOR RESIDUE GOL D 803

Chain	Residue
D	GLY142
D	PRO144
D	GLU147
D	TYR177
D	LYS217
D	GOL804
D	HOH1226
D	HOH1608

---

site_id	EC4
Number of Residues	5
Details	BINDING SITE FOR RESIDUE GOL D 804

Chain	Residue
D	LYS150
D	TYR177
D	GOL803
D	HOH1322
D	HOH1529

---

site_id	EC5
Number of Residues	10
Details	BINDING SITE FOR RESIDUE GOL D 806

Chain	Residue
B	LYS62
B	ALA63
B	GLN66
B	PRO73
B	HOH1010
B	HOH1348
B	HOH1625
D	LYS231
D	VAL232
D	GLY233

---

site_id	EC6
Number of Residues	11
Details	BINDING SITE FOR RESIDUE GOL D 807

Chain	Residue
C	GLY371
D	GLU368
D	LYS393
D	VAL412
D	ARG420
D	ASP422
D	ARG424
D	HOH1095
D	HOH1275
D	HOH1541
D	HOH1570

---

site_id	EC7
Number of Residues	3
Details	BINDING SITE FOR RESIDUE GOL D 808

Chain	Residue
D	PRO18
D	ALA19
D	ALA99

---

site_id	EC8
Number of Residues	6
Details	BINDING SITE FOR RESIDUE GOL D 809

Chain	Residue
D	HIS23
D	TYR64
D	LYS68
D	LYS265
D	ASP280
D	HOH1006

---

site_id	EC9
Number of Residues	2
Details	BINDING SITE FOR RESIDUE GOL D 810

Chain	Residue
D	TRP67
D	LYS68

---

site_id	FC1
Number of Residues	7
Details	BINDING SITE FOR RESIDUE GOL D 811

Chain	Residue
D	LEU56
D	ARG57
D	TYR80
D	LEU580
D	HOH1090
D	HOH1187
D	HOH1634

---

site_id	FC2
Number of Residues	5
Details	BINDING SITE FOR RESIDUE CU E 801

Chain	Residue
E	HIS456
E	HIS458
E	HIS624
E	PEO802
E	HOH1439

---

site_id	FC3
Number of Residues	8
Details	BINDING SITE FOR RESIDUE PEO E 802

Chain	Residue
E	TYY405
E	HIS456
E	HIS458
E	HIS624
E	MET634
E	CU801
E	HOH903
E	HOH1439

---

site_id	FC4
Number of Residues	6
Details	BINDING SITE FOR RESIDUE GOL E 803

Chain	Residue
E	GLY142
E	PRO144
E	GLU147
E	TYR177
E	LYS217
E	HOH1249

---

site_id	FC5
Number of Residues	7
Details	BINDING SITE FOR RESIDUE GOL E 804

Chain	Residue
E	PHE252
E	LYS253
E	MET254
E	ILE342
E	HIS343
E	HOH1507
E	HOH1656

---

site_id	FC6
Number of Residues	7
Details	BINDING SITE FOR RESIDUE GOL E 805

Chain	Residue
E	GLU58
E	PRO59
E	TYR575
E	ASP577
E	ASN578
E	HOH947
E	HOH1524

---

site_id	FC7
Number of Residues	2
Details	BINDING SITE FOR RESIDUE GOL E 806

Chain	Residue
E	PRO484
F	PRO442

---

site_id	FC8
Number of Residues	6
Details	BINDING SITE FOR RESIDUE GOL E 807

Chain	Residue
E	HIS23
E	TYR64
E	LYS68
E	LYS265
E	ASP280
E	HOH975

---

site_id	FC9
Number of Residues	9
Details	BINDING SITE FOR RESIDUE GOL E 808

Chain	Residue
E	GLU368
E	LYS393
E	ARG420
E	ASP422
E	ARG424
E	HOH1036
E	HOH1307
E	HOH1450
F	GLY371

---

site_id	GC1
Number of Residues	5
Details	BINDING SITE FOR RESIDUE CU F 801

Chain	Residue
F	HIS456
F	HIS458
F	HIS624
F	PEO802
F	HOH1402

---

site_id	GC2
Number of Residues	8
Details	BINDING SITE FOR RESIDUE PEO F 802

Chain	Residue
F	TYY405
F	LEU429
F	HIS456
F	HIS458
F	HIS624
F	MET634
F	CU801
F	HOH986

---

site_id	GC3
Number of Residues	7
Details	BINDING SITE FOR RESIDUE GOL F 803

Chain	Residue
F	HIS23
F	TYR64
F	LYS68
F	LYS265
F	ASP280
F	HOH948
F	HOH1470

---

site_id	GC4
Number of Residues	7
Details	BINDING SITE FOR RESIDUE GOL F 804

Chain	Residue
E	GLY371
F	LYS393
F	VAL412
F	ARG420
F	ASP422
F	GOL805
F	HOH1039

---

site_id	GC5
Number of Residues	8
Details	BINDING SITE FOR RESIDUE GOL F 805

Chain	Residue
E	GLY371
E	ARG390
F	ASP337
F	CYS338
F	LYS339
F	GLU368
F	GOL804
F	HOH1338

---

site_id	GC6
Number of Residues	6
Details	BINDING SITE FOR RESIDUE GOL F 806

Chain	Residue
F	PHE252
F	LYS253
F	MET254
F	HIS343
F	HOH1202
F	HOH1530

---

site_id	GC7
Number of Residues	3
Details	BINDING SITE FOR RESIDUE GOL F 807

Chain	Residue
F	PRO484
F	TYR485
F	TYR499

---

site_id	GC8
Number of Residues	2
Details	BINDING SITE FOR RESIDUE PO4 F 808

Chain	Residue
F	HIS218
F	LYS219

---

site_id	GC9
Number of Residues	7
Details	BINDING SITE FOR RESIDUE GOL F 809

Chain	Residue
F	LEU512
F	ASP604
F	GLY605
F	HOH1072
F	HOH1382
F	HOH1566
F	HOH1628

---

site_id	HC1
Number of Residues	8
Details	BINDING SITE FOR RESIDUE GOL F 810

Chain	Residue
A	GLU110
F	ALA133
F	VAL203
F	ILE204
F	HOH1113
F	HOH1409
F	HOH1477
F	HOH1629

---

site_id	HC2
Number of Residues	8
Details	BINDING SITE FOR RESIDUE GOL F 811

Chain	Residue
A	LYS561
A	ASP593
A	GOL808
F	TYR160
F	LYS561
F	SER591
F	HOH996
F	HOH1517

---

site_id	HC3
Number of Residues	10
Details	BINDING SITE FOR RESIDUE GOL F 812

Chain	Residue
F	GLU58
F	PRO59
F	ARG61
F	TYR575
F	ASP577
F	ASN578
F	HOH925
F	HOH990
F	HOH1181
F	HOH1214

---

Functional Information from PROSITE/UniProt

site_id	PS01159
Number of Residues	26
Details	WW_DOMAIN_1 WW/rsp5/WWP domain signature. WgtgkrlqqalvYYrsdedd.SQYSHP

Chain	Residue	Details
A	TRP164-PRO189

---

site_id	PS01164
Number of Residues	14
Details	COPPER_AMINE_OXID_1 Copper amine oxidase topaquinone signature. LVVsqifTaaNYEY

Chain	Residue	Details
A	LEU394-TYR407

---

site_id	PS01165
Number of Residues	14
Details	COPPER_AMINE_OXID_2 Copper amine oxidase copper-binding site signature. TfGitHFpapEDfP

Chain	Residue	Details
A	THR619-PRO632

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	6
Details	Active site: {"description":"Proton acceptor","evidences":[{"source":"PubMed","id":"9551552","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	6
Details	Active site: {"description":"Schiff-base intermediate with substrate; via topaquinone","evidences":[{"source":"PubMed","id":"10933787","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"9551552","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1EKM","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3NBB","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3NBJ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3T0U","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4KFF","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	66
Details	Binding site: {"evidences":[{"source":"PDB","id":"4EV2","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4EV5","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	18
Details	Binding site: {"evidences":[{"source":"PubMed","id":"10933787","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"9551552","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1A2V","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1EKM","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2OOV","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2OQE","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3N9H","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3NBB","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3NBJ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3T0U","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4EV2","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4EV5","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4KFD","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4KFE","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4KFF","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	18
Details	Binding site: {"evidences":[{"source":"UniProtKB","id":"Q43077","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	6
Details	Modified residue: {"description":"2',4',5'-topaquinone","evidences":[{"source":"PubMed","id":"9551552","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1A2V","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2OQE","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	6
Details	Glycosylation: {"description":"N-linked (GlcNAc...) asparagine","evidences":[{"source":"PubMed","id":"9551552","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---