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4K6D

Crystal structure of Staphylococcal nuclease variant V23M/T62F at cryogenic temperature

Functional Information from GO Data
ChainGOidnamespacecontents
A0003676molecular_functionnucleic acid binding
A0004518molecular_functionnuclease activity
Functional Information from PDB Data
site_idAC1
Number of Residues15
DetailsBINDING SITE FOR RESIDUE THP A 201
ChainResidue
AARG35
ACA202
AHOH306
AHOH312
AHOH330
AHOH367
AHOH376
AASP40
ALYS71
ALYS84
ATYR85
AARG87
ALEU89
ATYR113
ATYR115

site_idAC2
Number of Residues6
DetailsBINDING SITE FOR RESIDUE CA A 202
ChainResidue
AASP21
AASP40
ATHR41
ATHP201
AHOH366
AHOH367

site_idAC3
Number of Residues5
DetailsBINDING SITE FOR RESIDUE MPD A 203
ChainResidue
ALEU14
AILE15
ALYS24
AGLU67
AHOH305

site_idAC4
Number of Residues3
DetailsBINDING SITE FOR RESIDUE MPD A 205
ChainResidue
APHE61
ALYS116
AHOH373

Functional Information from PROSITE/UniProt
site_idPS01123
Number of Residues25
DetailsTNASE_1 Thermonuclease family signature 1. DGDTMkLmykgqpmtf.RLllVDtPE
ChainResidueDetails
AASP19-GLU43

site_idPS01284
Number of Residues11
DetailsTNASE_2 Thermonuclease family signature 2. DKYGRgLAyIY
ChainResidueDetails
AASP83-TYR93

Functional Information from SwissProt/UniProt
site_idSWS_FT_FI1
Number of Residues3
DetailsACT_SITE: ACT_SITE => ECO:0000269|PubMed:288045
ChainResidueDetails
AARG35
AGLU43
AARG87

site_idSWS_FT_FI2
Number of Residues3
DetailsBINDING:
ChainResidueDetails
AASP21
AASP40
ATHR41

Catalytic Information from CSA
site_idMCSA1
Number of Residues6
DetailsM-CSA 165
ChainResidueDetails
AASP21metal ligand
AARG35electrostatic stabiliser, hydrogen bond donor
AASP40metal ligand
ATHR41metal ligand
AGLU43hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor
AARG87electrostatic stabiliser, hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor

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PDB entries from 2024-10-30

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