4JTC
Crystal structure of Kv1.2-2.1 paddle chimera channel in complex with Charybdotoxin in Cs+
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0002244 | biological_process | hematopoietic progenitor cell differentiation |
| A | 0004033 | molecular_function | aldo-keto reductase (NADPH) activity |
| A | 0005249 | molecular_function | voltage-gated potassium channel activity |
| A | 0005515 | molecular_function | protein binding |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0005829 | cellular_component | cytosol |
| A | 0005856 | cellular_component | cytoskeleton |
| A | 0005874 | cellular_component | microtubule |
| A | 0005886 | cellular_component | plasma membrane |
| A | 0006811 | biological_process | monoatomic ion transport |
| A | 0006813 | biological_process | potassium ion transport |
| A | 0008076 | cellular_component | voltage-gated potassium channel complex |
| A | 0009898 | cellular_component | cytoplasmic side of plasma membrane |
| A | 0014069 | cellular_component | postsynaptic density |
| A | 0015459 | molecular_function | potassium channel regulator activity |
| A | 0016020 | cellular_component | membrane |
| A | 0016491 | molecular_function | oxidoreductase activity |
| A | 0030424 | cellular_component | axon |
| A | 0031234 | cellular_component | extrinsic component of cytoplasmic side of plasma membrane |
| A | 0034220 | biological_process | monoatomic ion transmembrane transport |
| A | 0034702 | cellular_component | monoatomic ion channel complex |
| A | 0034705 | cellular_component | potassium channel complex |
| A | 0042995 | cellular_component | cell projection |
| A | 0043005 | cellular_component | neuron projection |
| A | 0043194 | cellular_component | axon initial segment |
| A | 0043679 | cellular_component | axon terminus |
| A | 0044224 | cellular_component | juxtaparanode region of axon |
| A | 0044325 | molecular_function | transmembrane transporter binding |
| A | 0044877 | molecular_function | protein-containing complex binding |
| A | 0045202 | cellular_component | synapse |
| A | 0045445 | biological_process | myoblast differentiation |
| A | 0050905 | biological_process | neuromuscular process |
| A | 0055085 | biological_process | transmembrane transport |
| A | 0055114 | biological_process | obsolete oxidation-reduction process |
| A | 0070995 | biological_process | NADPH oxidation |
| A | 0071805 | biological_process | potassium ion transmembrane transport |
| A | 0098839 | cellular_component | postsynaptic density membrane |
| A | 0098900 | biological_process | regulation of action potential |
| A | 0098978 | cellular_component | glutamatergic synapse |
| A | 1901379 | biological_process | regulation of potassium ion transmembrane transport |
| A | 1990031 | cellular_component | pinceau fiber |
| A | 2000008 | biological_process | regulation of protein localization to cell surface |
| B | 0005216 | molecular_function | monoatomic ion channel activity |
| B | 0005249 | molecular_function | voltage-gated potassium channel activity |
| B | 0006811 | biological_process | monoatomic ion transport |
| B | 0006813 | biological_process | potassium ion transport |
| B | 0008076 | cellular_component | voltage-gated potassium channel complex |
| B | 0016020 | cellular_component | membrane |
| B | 0051260 | biological_process | protein homooligomerization |
| B | 0055085 | biological_process | transmembrane transport |
| G | 0002244 | biological_process | hematopoietic progenitor cell differentiation |
| G | 0004033 | molecular_function | aldo-keto reductase (NADPH) activity |
| G | 0005249 | molecular_function | voltage-gated potassium channel activity |
| G | 0005515 | molecular_function | protein binding |
| G | 0005737 | cellular_component | cytoplasm |
| G | 0005829 | cellular_component | cytosol |
| G | 0005856 | cellular_component | cytoskeleton |
| G | 0005874 | cellular_component | microtubule |
| G | 0005886 | cellular_component | plasma membrane |
| G | 0006811 | biological_process | monoatomic ion transport |
| G | 0006813 | biological_process | potassium ion transport |
| G | 0008076 | cellular_component | voltage-gated potassium channel complex |
| G | 0009898 | cellular_component | cytoplasmic side of plasma membrane |
| G | 0014069 | cellular_component | postsynaptic density |
| G | 0015459 | molecular_function | potassium channel regulator activity |
| G | 0016020 | cellular_component | membrane |
| G | 0016491 | molecular_function | oxidoreductase activity |
| G | 0030424 | cellular_component | axon |
| G | 0031234 | cellular_component | extrinsic component of cytoplasmic side of plasma membrane |
| G | 0034220 | biological_process | monoatomic ion transmembrane transport |
| G | 0034702 | cellular_component | monoatomic ion channel complex |
| G | 0034705 | cellular_component | potassium channel complex |
| G | 0042995 | cellular_component | cell projection |
| G | 0043005 | cellular_component | neuron projection |
| G | 0043194 | cellular_component | axon initial segment |
| G | 0043679 | cellular_component | axon terminus |
| G | 0044224 | cellular_component | juxtaparanode region of axon |
| G | 0044325 | molecular_function | transmembrane transporter binding |
| G | 0044877 | molecular_function | protein-containing complex binding |
| G | 0045202 | cellular_component | synapse |
| G | 0045445 | biological_process | myoblast differentiation |
| G | 0050905 | biological_process | neuromuscular process |
| G | 0055085 | biological_process | transmembrane transport |
| G | 0055114 | biological_process | obsolete oxidation-reduction process |
| G | 0070995 | biological_process | NADPH oxidation |
| G | 0071805 | biological_process | potassium ion transmembrane transport |
| G | 0098839 | cellular_component | postsynaptic density membrane |
| G | 0098900 | biological_process | regulation of action potential |
| G | 0098978 | cellular_component | glutamatergic synapse |
| G | 1901379 | biological_process | regulation of potassium ion transmembrane transport |
| G | 1990031 | cellular_component | pinceau fiber |
| G | 2000008 | biological_process | regulation of protein localization to cell surface |
| H | 0005216 | molecular_function | monoatomic ion channel activity |
| H | 0005249 | molecular_function | voltage-gated potassium channel activity |
| H | 0006811 | biological_process | monoatomic ion transport |
| H | 0006813 | biological_process | potassium ion transport |
| H | 0008076 | cellular_component | voltage-gated potassium channel complex |
| H | 0016020 | cellular_component | membrane |
| H | 0051260 | biological_process | protein homooligomerization |
| H | 0055085 | biological_process | transmembrane transport |
| Y | 0005576 | cellular_component | extracellular region |
| Y | 0008200 | molecular_function | ion channel inhibitor activity |
| Y | 0015459 | molecular_function | potassium channel regulator activity |
| Y | 0031640 | biological_process | killing of cells of another organism |
| Y | 0035821 | biological_process | modulation of process of another organism |
| Y | 0042742 | biological_process | defense response to bacterium |
| Y | 0050832 | biological_process | defense response to fungus |
| Y | 0090729 | molecular_function | toxin activity |
| Y | 0099106 | molecular_function | ion channel regulator activity |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 26 |
| Details | BINDING SITE FOR RESIDUE NAP A 1001 |
| Chain | Residue |
| A | GLY55 |
| A | GLN214 |
| A | TRP243 |
| A | SER244 |
| A | PRO245 |
| A | LEU246 |
| A | CYS248 |
| A | GLY249 |
| A | LYS254 |
| A | TYR262 |
| A | SER263 |
| A | THR56 |
| A | ARG264 |
| A | LEU322 |
| A | GLY323 |
| A | SER325 |
| A | GLN329 |
| A | GLU332 |
| A | ASN333 |
| A | TRP57 |
| A | GLN63 |
| A | ASP85 |
| A | TYR90 |
| A | LYS118 |
| A | SER188 |
| A | ARG189 |
| site_id | AC2 |
| Number of Residues | 8 |
| Details | BINDING SITE FOR RESIDUE CS B 501 |
| Chain | Residue |
| B | GLY372 |
| B | GLY372 |
| B | GLY372 |
| B | GLY372 |
| B | TYR373 |
| B | TYR373 |
| B | TYR373 |
| B | TYR373 |
| site_id | AC3 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE CS B 502 |
| Chain | Residue |
| B | VAL371 |
| B | VAL371 |
| B | VAL371 |
| B | VAL371 |
| site_id | AC4 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE CS B 503 |
| Chain | Residue |
| B | THR370 |
| B | THR370 |
| B | THR370 |
| B | THR370 |
| site_id | AC5 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE PGW B 504 |
| Chain | Residue |
| B | ILE328 |
| B | PRO358 |
| B | PHE361 |
| B | ILE381 |
| B | LYS384 |
| B | SER388 |
| B | PGW509 |
| site_id | AC6 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE PGW B 505 |
| Chain | Residue |
| B | PGW506 |
| B | PGW507 |
| site_id | AC7 |
| Number of Residues | 1 |
| Details | BINDING SITE FOR RESIDUE PGW B 506 |
| Chain | Residue |
| B | PGW505 |
| site_id | AC8 |
| Number of Residues | 1 |
| Details | BINDING SITE FOR RESIDUE PGW B 507 |
| Chain | Residue |
| B | PGW505 |
| site_id | AC9 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE PGW B 509 |
| Chain | Residue |
| B | VAL178 |
| B | PGW504 |
| site_id | BC1 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE PGW B 510 |
| Chain | Residue |
| B | LEU313 |
| B | ILE328 |
| B | ALA393 |
| B | THR397 |
| B | PGW511 |
| B | PGW515 |
| site_id | BC2 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE PGW B 511 |
| Chain | Residue |
| B | MET321 |
| B | PGW510 |
| site_id | BC3 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE PGW B 512 |
| Chain | Residue |
| B | ILE187 |
| B | PHE188 |
| site_id | BC4 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE PGW B 513 |
| Chain | Residue |
| B | PRO221 |
| B | PHE222 |
| site_id | BC5 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE PGW B 514 |
| Chain | Residue |
| B | HIS306 |
| B | SER307 |
| B | LYS308 |
| B | ARG322 |
| B | GLY325 |
| B | LEU326 |
| B | PHE329 |
| site_id | BC6 |
| Number of Residues | 1 |
| Details | BINDING SITE FOR RESIDUE PGW B 515 |
| Chain | Residue |
| B | PGW510 |
| site_id | BC7 |
| Number of Residues | 8 |
| Details | BINDING SITE FOR RESIDUE PGW B 516 |
| Chain | Residue |
| B | PHE301 |
| B | LEU310 |
| B | GLN311 |
| B | GLY314 |
| B | GLN315 |
| B | LYS318 |
| B | ARG415 |
| B | GLU416 |
| site_id | BC8 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE PGW B 518 |
| Chain | Residue |
| B | ILE257 |
| B | MET295 |
| site_id | BC9 |
| Number of Residues | 30 |
| Details | BINDING SITE FOR RESIDUE NAP G 1001 |
| Chain | Residue |
| G | LEU246 |
| G | ALA247 |
| G | CYS248 |
| G | GLY249 |
| G | SER252 |
| G | LYS254 |
| G | TYR262 |
| G | SER263 |
| G | ARG264 |
| G | PRO304 |
| G | LEU322 |
| G | GLY323 |
| G | SER325 |
| G | GLN329 |
| G | GLU332 |
| G | ASN333 |
| G | GLY55 |
| G | THR56 |
| G | TRP57 |
| G | GLN63 |
| G | ASP85 |
| G | TYR90 |
| G | LYS118 |
| G | ASN158 |
| G | SER188 |
| G | ARG189 |
| G | GLN214 |
| G | TRP243 |
| G | SER244 |
| G | PRO245 |
| site_id | CC1 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE CS H 501 |
| Chain | Residue |
| H | GLY372 |
| H | GLY372 |
| H | GLY372 |
| H | GLY372 |
| site_id | CC2 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE CS H 502 |
| Chain | Residue |
| H | THR370 |
| H | THR370 |
| H | THR370 |
| H | THR370 |
| site_id | CC3 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE CS H 503 |
| Chain | Residue |
| H | THR370 |
| H | THR370 |
| H | THR370 |
| H | THR370 |
| site_id | CC4 |
| Number of Residues | 8 |
| Details | BINDING SITE FOR RESIDUE PGW H 504 |
| Chain | Residue |
| H | PRO358 |
| H | ASP359 |
| H | PHE361 |
| H | TRP362 |
| H | VAL365 |
| H | ILE381 |
| H | LYS384 |
| H | SER388 |
Functional Information from PROSITE/UniProt
| site_id | PS01138 |
| Number of Residues | 23 |
| Details | SCORP_SHORT_TOXIN Scorpion short toxins signature. CwsvCqrlhntsr.GKCMnkkCrC |
| Chain | Residue | Details |
| Y | CYS13-CYS35 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 1 |
| Details | SITE: Basic residue of the functional dyad => ECO:0000250 |
| Chain | Residue | Details |
| Y | LYS27 | |
| B | CYS244-ILE254 | |
| B | SER307-MET321 | |
| H | MET1-GLY160 | |
| H | CYS244-ILE254 | |
| H | SER307-MET321 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 1 |
| Details | SITE: Aromatic residue of the functional dyad => ECO:0000250 |
| Chain | Residue | Details |
| Y | TYR36 | |
| A | LYS254 | |
| A | ARG264 | |
| A | SER325 | |
| A | GLN329 | |
| A | ASN333 | |
| G | THR56 | |
| G | TRP57 | |
| G | GLN63 | |
| G | ASP85 | |
| G | SER188 | |
| H | PRO161-LEU182 | |
| G | GLN214 | |
| G | TRP243 | |
| G | SER244 | |
| G | LEU246 | |
| G | LYS254 | |
| G | ARG264 | |
| G | SER325 | |
| G | GLN329 | |
| G | ASN333 | |
| A | GLN63 | |
| A | ASP85 | |
| A | SER188 | |
| A | GLN214 | |
| A | TRP243 | |
| A | SER244 | |
| A | LEU246 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 1 |
| Details | MOD_RES: Pyrrolidone carboxylic acid => ECO:0000269|PubMed:2453055, ECO:0000269|PubMed:2482078 |
| Chain | Residue | Details |
| Y | PCA1 | |
| H | GLU183-PRO221 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 42 |
| Details | TRANSMEM: Helical; Name=Segment S2 => ECO:0000269|PubMed:18004376, ECO:0000269|PubMed:20360102, ECO:0000269|PubMed:20534430 |
| Chain | Residue | Details |
| B | PHE222-ALA243 | |
| H | PHE222-ALA243 |
| site_id | SWS_FT_FI5 |
| Number of Residues | 42 |
| Details | TRANSMEM: Helical; Name=Segment S5 => ECO:0000269|PubMed:18004376, ECO:0000269|PubMed:20360102, ECO:0000269|PubMed:20534430 |
| Chain | Residue | Details |
| B | ARG322-TYR343 | |
| H | ARG322-TYR343 |
| site_id | SWS_FT_FI6 |
| Number of Residues | 38 |
| Details | TOPO_DOM: Extracellular => ECO:0000269|PubMed:18004376, ECO:0000269|PubMed:20360102, ECO:0000269|PubMed:20534430 |
| Chain | Residue | Details |
| B | PHE344-ILE357 | |
| B | PRO378-LYS384 | |
| H | PHE344-ILE357 | |
| H | PRO378-LYS384 |
| site_id | SWS_FT_FI7 |
| Number of Residues | 22 |
| Details | INTRAMEM: Helical; Name=Pore helix => ECO:0000269|PubMed:18004376, ECO:0000269|PubMed:20360102, ECO:0000269|PubMed:20534430 |
| Chain | Residue | Details |
| B | PRO358-THR369 | |
| H | PRO358-THR369 |
| site_id | SWS_FT_FI8 |
| Number of Residues | 14 |
| Details | INTRAMEM: INTRAMEM => ECO:0000269|PubMed:18004376, ECO:0000269|PubMed:20360102, ECO:0000269|PubMed:20534430 |
| Chain | Residue | Details |
| B | THR370-VAL377 | |
| H | THR370-VAL377 |
| site_id | SWS_FT_FI9 |
| Number of Residues | 56 |
| Details | TRANSMEM: Helical; Name=Segment S6 => ECO:0000269|PubMed:18004376, ECO:0000269|PubMed:20360102, ECO:0000269|PubMed:20534430 |
| Chain | Residue | Details |
| B | ILE385-TYR413 | |
| H | ILE385-TYR413 |
| site_id | SWS_FT_FI10 |
| Number of Residues | 162 |
| Details | TOPO_DOM: Cytoplasmic => ECO:0000269|PubMed:12151401, ECO:0000305 |
| Chain | Residue | Details |
| B | HIS414-VAL495 | |
| H | HIS414-VAL495 |
| site_id | SWS_FT_FI11 |
| Number of Residues | 2 |
| Details | SITE: Important for normal, slow channel gating => ECO:0000269|PubMed:17766348 |
| Chain | Residue | Details |
| B | THR252 | |
| H | THR252 |
| site_id | SWS_FT_FI12 |
| Number of Residues | 2 |
| Details | SITE: Important for binding with the scorpion mesomartoxin; when the scorpion mesomartoxin-rKv1.2/KCNA2 interaction is modeled, this residue is close to the 'Y-57' residue of the toxin => ECO:0000305|PubMed:25514171 |
| Chain | Residue | Details |
| B | VAL377 | |
| H | VAL377 |
| site_id | SWS_FT_FI13 |
| Number of Residues | 2 |
| Details | MOD_RES: Phosphotyrosine => ECO:0000250|UniProtKB:P63141 |
| Chain | Residue | Details |
| B | TYR425 | |
| H | TYR425 |
| site_id | SWS_FT_FI14 |
| Number of Residues | 2 |
| Details | MOD_RES: Phosphoserine => ECO:0000250|UniProtKB:P63141 |
| Chain | Residue | Details |
| B | SER430 | |
| H | SER430 |
| site_id | SWS_FT_FI15 |
| Number of Residues | 2 |
| Details | MOD_RES: Phosphoserine => ECO:0000269|PubMed:18003609, ECO:0007744|PubMed:22673903 |
| Chain | Residue | Details |
| B | SER436 | |
| H | SER436 |
| site_id | SWS_FT_FI16 |
| Number of Residues | 4 |
| Details | MOD_RES: Phosphoserine => ECO:0007744|PubMed:22673903 |
| Chain | Residue | Details |
| B | SER437 | |
| B | SER464 | |
| H | SER437 | |
| H | SER464 |
| site_id | SWS_FT_FI17 |
| Number of Residues | 2 |
| Details | MOD_RES: Phosphoserine => ECO:0000269|PubMed:18003609, ECO:0000269|PubMed:21602278 |
| Chain | Residue | Details |
| B | SER445 | |
| H | SER445 |
| site_id | SWS_FT_FI18 |
| Number of Residues | 2 |
| Details | MOD_RES: Phosphotyrosine => ECO:0000305|PubMed:21602278 |
| Chain | Residue | Details |
| B | TYR454 | |
| H | TYR454 |
| site_id | SWS_FT_FI19 |
| Number of Residues | 2 |
| Details | LIPID: S-palmitoyl cysteine => ECO:0000255 |
| Chain | Residue | Details |
| B | CYS244 | |
| H | CYS244 |
| site_id | SWS_FT_FI20 |
| Number of Residues | 2 |
| Details | CARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000255, ECO:0000269|PubMed:16770729 |
| Chain | Residue | Details |
| B | GLN207 | |
| H | GLN207 |
| site_id | SWS_FT_FI21 |
| Number of Residues | 26 |
| Details | TOPO_DOM: Extracellular => ECO:0000250|UniProtKB:P63142 |
| Chain | Residue | Details |
| B | GLU274-ARG287 | |
| H | GLU274-ARG287 |
