4IME

Crystal Structure of Pasteurella multocida N-Acetyl-D-Neuraminic acid lyase K164A Mutant

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0005975	biological_process	carbohydrate metabolic process
A	0008747	molecular_function	N-acetylneuraminate lyase activity
A	0016829	molecular_function	lyase activity
A	0019262	biological_process	N-acetylneuraminate catabolic process
B	0005737	cellular_component	cytoplasm
B	0005829	cellular_component	cytosol
B	0005975	biological_process	carbohydrate metabolic process
B	0008747	molecular_function	N-acetylneuraminate lyase activity
B	0016829	molecular_function	lyase activity
B	0019262	biological_process	N-acetylneuraminate catabolic process
C	0005737	cellular_component	cytoplasm
C	0005829	cellular_component	cytosol
C	0005975	biological_process	carbohydrate metabolic process
C	0008747	molecular_function	N-acetylneuraminate lyase activity
C	0016829	molecular_function	lyase activity
C	0019262	biological_process	N-acetylneuraminate catabolic process
D	0005737	cellular_component	cytoplasm
D	0005829	cellular_component	cytosol
D	0005975	biological_process	carbohydrate metabolic process
D	0008747	molecular_function	N-acetylneuraminate lyase activity
D	0016829	molecular_function	lyase activity
D	0019262	biological_process	N-acetylneuraminate catabolic process
E	0005737	cellular_component	cytoplasm
E	0005829	cellular_component	cytosol
E	0005975	biological_process	carbohydrate metabolic process
E	0008747	molecular_function	N-acetylneuraminate lyase activity
E	0016829	molecular_function	lyase activity
E	0019262	biological_process	N-acetylneuraminate catabolic process
F	0005737	cellular_component	cytoplasm
F	0005829	cellular_component	cytosol
F	0005975	biological_process	carbohydrate metabolic process
F	0008747	molecular_function	N-acetylneuraminate lyase activity
F	0016829	molecular_function	lyase activity
F	0019262	biological_process	N-acetylneuraminate catabolic process
G	0005737	cellular_component	cytoplasm
G	0005829	cellular_component	cytosol
G	0005975	biological_process	carbohydrate metabolic process
G	0008747	molecular_function	N-acetylneuraminate lyase activity
G	0016829	molecular_function	lyase activity
G	0019262	biological_process	N-acetylneuraminate catabolic process
H	0005737	cellular_component	cytoplasm
H	0005829	cellular_component	cytosol
H	0005975	biological_process	carbohydrate metabolic process
H	0008747	molecular_function	N-acetylneuraminate lyase activity
H	0016829	molecular_function	lyase activity
H	0019262	biological_process	N-acetylneuraminate catabolic process

Functional Information from PDB Data

site_id	AC1
Number of Residues	9
Details	BINDING SITE FOR RESIDUE ME2 B 301

Chain	Residue
A	LYS112
A	PRO139
A	PHE140
A	LEU141
A	GLY143
B	LYS112
B	PHE140
B	LEU141
B	HOH736

---

site_id	AC2
Number of Residues	10
Details	BINDING SITE FOR RESIDUE ME2 B 302

Chain	Residue
A	ASN84
A	GLN86
B	ASP18
B	GLY19
B	SER20
B	SER55
B	GLU58
B	ARG269
B	HOH484
B	HOH666

---

site_id	AC3
Number of Residues	13
Details	BINDING SITE FOR RESIDUE ME2 C 5001

Chain	Residue
C	LYS112
C	PRO139
C	PHE140
C	LEU141
C	GLY143
C	HOH5384
D	LYS112
D	PRO139
D	PHE140
D	LEU141
D	GLY143
D	HOH673
D	HOH756

---

site_id	AC4
Number of Residues	8
Details	BINDING SITE FOR RESIDUE ME2 D 301

Chain	Residue
D	HOH755
D	HOH766
D	HOH767
D	HOH769
F	LYS5
F	ASP202
F	HOH534
F	HOH548

---

site_id	AC5
Number of Residues	10
Details	BINDING SITE FOR RESIDUE ME2 E 301

Chain	Residue
E	LYS112
E	PHE140
E	LEU141
E	GLY143
E	HOH712
F	LYS112
F	PRO139
F	PHE140
F	LEU141
F	GLY143

---

site_id	AC6
Number of Residues	8
Details	BINDING SITE FOR RESIDUE ME2 E 302

Chain	Residue
E	LYS93
E	HOH446
E	HOH617
H	ASP18
H	SER20
H	GLU58
H	ARG269
H	HOH395

---

site_id	AC7
Number of Residues	9
Details	BINDING SITE FOR RESIDUE ME2 G 301

Chain	Residue
G	LYS112
G	PRO139
G	PHE140
G	LEU141
G	GLY143
G	HOH662
H	PHE140
H	GLY143
H	HOH615

---

site_id	AC8
Number of Residues	5
Details	BINDING SITE FOR RESIDUE PG6 G 302

Chain	Residue
G	LEU246
G	ALA247
G	ASN248
G	LYS280
G	HOH571

---

Functional Information from PROSITE/UniProt

site_id	PS00217
Number of Residues	26
Details	SUGAR_TRANSPORT_2 Sugar transport proteins signature 2. VdGLYvGGstgenfmLstEekkeifR

Chain	Residue	Details
A	VAL39-ARG64

---

site_id	PS00665
Number of Residues	18
Details	DHDPS_1 Dihydrodipicolinate synthase signature 1. GLYvgGSTGENfmlsteE

Chain	Residue	Details
A	GLY41-GLU58

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	8
Details	Active site: {"description":"Proton donor","evidences":[{"source":"HAMAP-Rule","id":"MF_01237","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	8
Details	Active site: {"description":"Schiff-base intermediate with substrate","evidences":[{"source":"HAMAP-Rule","id":"MF_01237","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"24152047","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	72
Details	Binding site: {"evidences":[{"source":"PubMed","id":"24152047","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"4IMF","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---