4IME
Crystal Structure of Pasteurella multocida N-Acetyl-D-Neuraminic acid lyase K164A Mutant
Functional Information from GO Data
Chain | GOid | namespace | contents |
A | 0005737 | cellular_component | cytoplasm |
A | 0005829 | cellular_component | cytosol |
A | 0005975 | biological_process | carbohydrate metabolic process |
A | 0008747 | molecular_function | N-acetylneuraminate lyase activity |
A | 0016829 | molecular_function | lyase activity |
A | 0019262 | biological_process | N-acetylneuraminate catabolic process |
B | 0005737 | cellular_component | cytoplasm |
B | 0005829 | cellular_component | cytosol |
B | 0005975 | biological_process | carbohydrate metabolic process |
B | 0008747 | molecular_function | N-acetylneuraminate lyase activity |
B | 0016829 | molecular_function | lyase activity |
B | 0019262 | biological_process | N-acetylneuraminate catabolic process |
C | 0005737 | cellular_component | cytoplasm |
C | 0005829 | cellular_component | cytosol |
C | 0005975 | biological_process | carbohydrate metabolic process |
C | 0008747 | molecular_function | N-acetylneuraminate lyase activity |
C | 0016829 | molecular_function | lyase activity |
C | 0019262 | biological_process | N-acetylneuraminate catabolic process |
D | 0005737 | cellular_component | cytoplasm |
D | 0005829 | cellular_component | cytosol |
D | 0005975 | biological_process | carbohydrate metabolic process |
D | 0008747 | molecular_function | N-acetylneuraminate lyase activity |
D | 0016829 | molecular_function | lyase activity |
D | 0019262 | biological_process | N-acetylneuraminate catabolic process |
E | 0005737 | cellular_component | cytoplasm |
E | 0005829 | cellular_component | cytosol |
E | 0005975 | biological_process | carbohydrate metabolic process |
E | 0008747 | molecular_function | N-acetylneuraminate lyase activity |
E | 0016829 | molecular_function | lyase activity |
E | 0019262 | biological_process | N-acetylneuraminate catabolic process |
F | 0005737 | cellular_component | cytoplasm |
F | 0005829 | cellular_component | cytosol |
F | 0005975 | biological_process | carbohydrate metabolic process |
F | 0008747 | molecular_function | N-acetylneuraminate lyase activity |
F | 0016829 | molecular_function | lyase activity |
F | 0019262 | biological_process | N-acetylneuraminate catabolic process |
G | 0005737 | cellular_component | cytoplasm |
G | 0005829 | cellular_component | cytosol |
G | 0005975 | biological_process | carbohydrate metabolic process |
G | 0008747 | molecular_function | N-acetylneuraminate lyase activity |
G | 0016829 | molecular_function | lyase activity |
G | 0019262 | biological_process | N-acetylneuraminate catabolic process |
H | 0005737 | cellular_component | cytoplasm |
H | 0005829 | cellular_component | cytosol |
H | 0005975 | biological_process | carbohydrate metabolic process |
H | 0008747 | molecular_function | N-acetylneuraminate lyase activity |
H | 0016829 | molecular_function | lyase activity |
H | 0019262 | biological_process | N-acetylneuraminate catabolic process |
Functional Information from PDB Data
site_id | AC1 |
Number of Residues | 9 |
Details | BINDING SITE FOR RESIDUE ME2 B 301 |
Chain | Residue |
A | LYS112 |
A | PRO139 |
A | PHE140 |
A | LEU141 |
A | GLY143 |
B | LYS112 |
B | PHE140 |
B | LEU141 |
B | HOH736 |
site_id | AC2 |
Number of Residues | 10 |
Details | BINDING SITE FOR RESIDUE ME2 B 302 |
Chain | Residue |
A | ASN84 |
A | GLN86 |
B | ASP18 |
B | GLY19 |
B | SER20 |
B | SER55 |
B | GLU58 |
B | ARG269 |
B | HOH484 |
B | HOH666 |
site_id | AC3 |
Number of Residues | 13 |
Details | BINDING SITE FOR RESIDUE ME2 C 5001 |
Chain | Residue |
C | LYS112 |
C | PRO139 |
C | PHE140 |
C | LEU141 |
C | GLY143 |
C | HOH5384 |
D | LYS112 |
D | PRO139 |
D | PHE140 |
D | LEU141 |
D | GLY143 |
D | HOH673 |
D | HOH756 |
site_id | AC4 |
Number of Residues | 8 |
Details | BINDING SITE FOR RESIDUE ME2 D 301 |
Chain | Residue |
D | HOH755 |
D | HOH766 |
D | HOH767 |
D | HOH769 |
F | LYS5 |
F | ASP202 |
F | HOH534 |
F | HOH548 |
site_id | AC5 |
Number of Residues | 10 |
Details | BINDING SITE FOR RESIDUE ME2 E 301 |
Chain | Residue |
E | LYS112 |
E | PHE140 |
E | LEU141 |
E | GLY143 |
E | HOH712 |
F | LYS112 |
F | PRO139 |
F | PHE140 |
F | LEU141 |
F | GLY143 |
site_id | AC6 |
Number of Residues | 8 |
Details | BINDING SITE FOR RESIDUE ME2 E 302 |
Chain | Residue |
E | LYS93 |
E | HOH446 |
E | HOH617 |
H | ASP18 |
H | SER20 |
H | GLU58 |
H | ARG269 |
H | HOH395 |
site_id | AC7 |
Number of Residues | 9 |
Details | BINDING SITE FOR RESIDUE ME2 G 301 |
Chain | Residue |
G | LYS112 |
G | PRO139 |
G | PHE140 |
G | LEU141 |
G | GLY143 |
G | HOH662 |
H | PHE140 |
H | GLY143 |
H | HOH615 |
site_id | AC8 |
Number of Residues | 5 |
Details | BINDING SITE FOR RESIDUE PG6 G 302 |
Chain | Residue |
G | LEU246 |
G | ALA247 |
G | ASN248 |
G | LYS280 |
G | HOH571 |
Functional Information from PROSITE/UniProt
Functional Information from SwissProt/UniProt
site_id | SWS_FT_FI1 |
Number of Residues | 8 |
Details | ACT_SITE: Proton donor => ECO:0000255|HAMAP-Rule:MF_01237 |
Chain | Residue | Details |
A | TYR136 | |
B | TYR136 | |
C | TYR136 | |
D | TYR136 | |
E | TYR136 | |
F | TYR136 | |
G | TYR136 | |
H | TYR136 |
site_id | SWS_FT_FI2 |
Number of Residues | 8 |
Details | ACT_SITE: Schiff-base intermediate with substrate => ECO:0000255|HAMAP-Rule:MF_01237, ECO:0000305|PubMed:24152047 |
Chain | Residue | Details |
A | ALA164 | |
B | ALA164 | |
C | ALA164 | |
D | ALA164 | |
E | ALA164 | |
F | ALA164 | |
G | ALA164 | |
H | ALA164 |
site_id | SWS_FT_FI3 |
Number of Residues | 72 |
Details | BINDING: BINDING => ECO:0000269|PubMed:24152047, ECO:0007744|PDB:4IMF |
Chain | Residue | Details |
A | SER47 | |
B | SER47 | |
B | THR48 | |
B | TYR136 | |
B | THR166 | |
B | GLY188 | |
B | ASP190 | |
B | GLU191 | |
B | SER207 | |
B | TYR251 | |
C | SER47 | |
A | THR48 | |
C | THR48 | |
C | TYR136 | |
C | THR166 | |
C | GLY188 | |
C | ASP190 | |
C | GLU191 | |
C | SER207 | |
C | TYR251 | |
D | SER47 | |
D | THR48 | |
A | TYR136 | |
D | TYR136 | |
D | THR166 | |
D | GLY188 | |
D | ASP190 | |
D | GLU191 | |
D | SER207 | |
D | TYR251 | |
E | SER47 | |
E | THR48 | |
E | TYR136 | |
A | THR166 | |
E | THR166 | |
E | GLY188 | |
E | ASP190 | |
E | GLU191 | |
E | SER207 | |
E | TYR251 | |
F | SER47 | |
F | THR48 | |
F | TYR136 | |
F | THR166 | |
A | GLY188 | |
F | GLY188 | |
F | ASP190 | |
F | GLU191 | |
F | SER207 | |
F | TYR251 | |
G | SER47 | |
G | THR48 | |
G | TYR136 | |
G | THR166 | |
G | GLY188 | |
A | ASP190 | |
G | ASP190 | |
G | GLU191 | |
G | SER207 | |
G | TYR251 | |
H | SER47 | |
H | THR48 | |
H | TYR136 | |
H | THR166 | |
H | GLY188 | |
H | ASP190 | |
A | GLU191 | |
H | GLU191 | |
H | SER207 | |
H | TYR251 | |
A | SER207 | |
A | TYR251 |