Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

4IME

Crystal Structure of Pasteurella multocida N-Acetyl-D-Neuraminic acid lyase K164A Mutant

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0005975	biological_process	carbohydrate metabolic process
A	0008747	molecular_function	N-acetylneuraminate lyase activity
A	0016829	molecular_function	lyase activity
A	0019262	biological_process	N-acetylneuraminate catabolic process
B	0005737	cellular_component	cytoplasm
B	0005829	cellular_component	cytosol
B	0005975	biological_process	carbohydrate metabolic process
B	0008747	molecular_function	N-acetylneuraminate lyase activity
B	0016829	molecular_function	lyase activity
B	0019262	biological_process	N-acetylneuraminate catabolic process
C	0005737	cellular_component	cytoplasm
C	0005829	cellular_component	cytosol
C	0005975	biological_process	carbohydrate metabolic process
C	0008747	molecular_function	N-acetylneuraminate lyase activity
C	0016829	molecular_function	lyase activity
C	0019262	biological_process	N-acetylneuraminate catabolic process
D	0005737	cellular_component	cytoplasm
D	0005829	cellular_component	cytosol
D	0005975	biological_process	carbohydrate metabolic process
D	0008747	molecular_function	N-acetylneuraminate lyase activity
D	0016829	molecular_function	lyase activity
D	0019262	biological_process	N-acetylneuraminate catabolic process
E	0005737	cellular_component	cytoplasm
E	0005829	cellular_component	cytosol
E	0005975	biological_process	carbohydrate metabolic process
E	0008747	molecular_function	N-acetylneuraminate lyase activity
E	0016829	molecular_function	lyase activity
E	0019262	biological_process	N-acetylneuraminate catabolic process
F	0005737	cellular_component	cytoplasm
F	0005829	cellular_component	cytosol
F	0005975	biological_process	carbohydrate metabolic process
F	0008747	molecular_function	N-acetylneuraminate lyase activity
F	0016829	molecular_function	lyase activity
F	0019262	biological_process	N-acetylneuraminate catabolic process
G	0005737	cellular_component	cytoplasm
G	0005829	cellular_component	cytosol
G	0005975	biological_process	carbohydrate metabolic process
G	0008747	molecular_function	N-acetylneuraminate lyase activity
G	0016829	molecular_function	lyase activity
G	0019262	biological_process	N-acetylneuraminate catabolic process
H	0005737	cellular_component	cytoplasm
H	0005829	cellular_component	cytosol
H	0005975	biological_process	carbohydrate metabolic process
H	0008747	molecular_function	N-acetylneuraminate lyase activity
H	0016829	molecular_function	lyase activity
H	0019262	biological_process	N-acetylneuraminate catabolic process

Functional Information from PDB Data

site_id	AC1
Number of Residues	9
Details	BINDING SITE FOR RESIDUE ME2 B 301

Chain	Residue
A	LYS112
A	PRO139
A	PHE140
A	LEU141
A	GLY143
B	LYS112
B	PHE140
B	LEU141
B	HOH736

site_id	AC2
Number of Residues	10
Details	BINDING SITE FOR RESIDUE ME2 B 302

Chain	Residue
A	ASN84
A	GLN86
B	ASP18
B	GLY19
B	SER20
B	SER55
B	GLU58
B	ARG269
B	HOH484
B	HOH666

site_id	AC3
Number of Residues	13
Details	BINDING SITE FOR RESIDUE ME2 C 5001

Chain	Residue
C	LYS112
C	PRO139
C	PHE140
C	LEU141
C	GLY143
C	HOH5384
D	LYS112
D	PRO139
D	PHE140
D	LEU141
D	GLY143
D	HOH673
D	HOH756

site_id	AC4
Number of Residues	8
Details	BINDING SITE FOR RESIDUE ME2 D 301

Chain	Residue
D	HOH755
D	HOH766
D	HOH767
D	HOH769
F	LYS5
F	ASP202
F	HOH534
F	HOH548

site_id	AC5
Number of Residues	10
Details	BINDING SITE FOR RESIDUE ME2 E 301

Chain	Residue
E	LYS112
E	PHE140
E	LEU141
E	GLY143
E	HOH712
F	LYS112
F	PRO139
F	PHE140
F	LEU141
F	GLY143

site_id	AC6
Number of Residues	8
Details	BINDING SITE FOR RESIDUE ME2 E 302

Chain	Residue
E	LYS93
E	HOH446
E	HOH617
H	ASP18
H	SER20
H	GLU58
H	ARG269
H	HOH395

site_id	AC7
Number of Residues	9
Details	BINDING SITE FOR RESIDUE ME2 G 301

Chain	Residue
G	LYS112
G	PRO139
G	PHE140
G	LEU141
G	GLY143
G	HOH662
H	PHE140
H	GLY143
H	HOH615

site_id	AC8
Number of Residues	5
Details	BINDING SITE FOR RESIDUE PG6 G 302

Chain	Residue
G	LEU246
G	ALA247
G	ASN248
G	LYS280
G	HOH571

Functional Information from PROSITE/UniProt

site_id	PS00217
Number of Residues	26
Details	SUGAR_TRANSPORT_2 Sugar transport proteins signature 2. VdGLYvGGstgenfmLstEekkeifR

Chain	Residue	Details
A	VAL39-ARG64

site_id	PS00665
Number of Residues	18
Details	DHDPS_1 Dihydrodipicolinate synthase signature 1. GLYvgGSTGENfmlsteE

Chain	Residue	Details
A	GLY41-GLU58

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	8
Details	ACT_SITE: Proton donor => ECO:0000255\|HAMAP-Rule:MF_01237

Chain	Residue	Details
A	TYR136
B	TYR136
C	TYR136
D	TYR136
E	TYR136
F	TYR136
G	TYR136
H	TYR136

site_id	SWS_FT_FI2
Number of Residues	8
Details	ACT_SITE: Schiff-base intermediate with substrate => ECO:0000255\|HAMAP-Rule:MF_01237, ECO:0000305\|PubMed:24152047

Chain	Residue	Details
A	ALA164
B	ALA164
C	ALA164
D	ALA164
E	ALA164
F	ALA164
G	ALA164
H	ALA164

site_id	SWS_FT_FI3
Number of Residues	72
Details	BINDING: BINDING => ECO:0000269\|PubMed:24152047, ECO:0007744\|PDB:4IMF

Chain	Residue	Details
A	SER47
B	SER47
B	THR48
B	TYR136
B	THR166
B	GLY188
B	ASP190
B	GLU191
B	SER207
B	TYR251
C	SER47
A	THR48
C	THR48
C	TYR136
C	THR166
C	GLY188
C	ASP190
C	GLU191
C	SER207
C	TYR251
D	SER47
D	THR48
A	TYR136
D	TYR136
D	THR166
D	GLY188
D	ASP190
D	GLU191
D	SER207
D	TYR251
E	SER47
E	THR48
E	TYR136
A	THR166
E	THR166
E	GLY188
E	ASP190
E	GLU191
E	SER207
E	TYR251
F	SER47
F	THR48
F	TYR136
F	THR166
A	GLY188
F	GLY188
F	ASP190
F	GLU191
F	SER207
F	TYR251
G	SER47
G	THR48
G	TYR136
G	THR166
G	GLY188
A	ASP190
G	ASP190
G	GLU191
G	SER207
G	TYR251
H	SER47
H	THR48
H	TYR136
H	THR166
H	GLY188
H	ASP190
A	GLU191
H	GLU191
H	SER207
H	TYR251
A	SER207
A	TYR251

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)