4IHM

G215S, A251G, T257A, D260G, T262D mutant of carboxypeptidase T from Thermoactinomyces vulgaris

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004181	molecular_function	metallocarboxypeptidase activity
A	0006508	biological_process	proteolysis
A	0008270	molecular_function	zinc ion binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	7
Details	BINDING SITE FOR RESIDUE SO4 A 401

Chain	Residue
A	HIS69
A	ARG129
A	ASN146
A	ARG147
A	TYR255
A	GLU277
A	GOL413

---

site_id	AC2
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN A 402

Chain	Residue
A	HIS204
A	HOH680
A	HIS69
A	GLU72

---

site_id	AC3
Number of Residues	6
Details	BINDING SITE FOR RESIDUE CA A 403

Chain	Residue
A	ASP56
A	GLU57
A	GLU61
A	GLU104
A	HOH709
A	HOH710

---

site_id	AC4
Number of Residues	5
Details	BINDING SITE FOR RESIDUE CA A 404

Chain	Residue
A	SER50
A	ASP51
A	GLU57
A	GLU59
A	HOH650

---

site_id	AC5
Number of Residues	4
Details	BINDING SITE FOR RESIDUE CA A 405

Chain	Residue
A	ASP51
A	GLU57
A	GLU59
A	ASN101

---

site_id	AC6
Number of Residues	6
Details	BINDING SITE FOR RESIDUE CA A 406

Chain	Residue
A	SER7
A	TYR9
A	GLU14
A	HOH581
A	HOH687
A	HOH728

---

site_id	AC7
Number of Residues	7
Details	BINDING SITE FOR RESIDUE SO4 A 407

Chain	Residue
A	VAL16
A	ASN20
A	ASN25
A	LYS32
A	TRP45
A	HOH505
A	HOH807

---

site_id	AC8
Number of Residues	7
Details	BINDING SITE FOR RESIDUE SO4 A 408

Chain	Residue
A	PHE174
A	ARG183
A	ASN187
A	TRP264
A	GLN268
A	HOH681
A	HOH706

---

site_id	AC9
Number of Residues	6
Details	BINDING SITE FOR RESIDUE GOL A 409

Chain	Residue
A	TYR118
A	ARG132
A	HOH513
A	HOH714
A	HOH715
A	HOH817

---

site_id	BC1
Number of Residues	5
Details	BINDING SITE FOR RESIDUE GOL A 410

Chain	Residue
A	HOH521
A	HOH538
A	HOH754
A	HOH774
A	HOH796

---

site_id	BC2
Number of Residues	7
Details	BINDING SITE FOR RESIDUE GOL A 411

Chain	Residue
A	ARG71
A	LYS152
A	CYS156
A	TYR216
A	HOH573
A	HOH740
A	HOH792

---

site_id	BC3
Number of Residues	8
Details	BINDING SITE FOR RESIDUE GOL A 412

Chain	Residue
A	HIS10
A	LYS18
A	GLU77
A	LEU80
A	TYR81
A	ASP84
A	HOH624
A	HOH773

---

site_id	BC4
Number of Residues	10
Details	BINDING SITE FOR RESIDUE GOL A 413

Chain	Residue
A	ASN146
A	THR202
A	MET261
A	ASP262
A	THR275
A	SO4401
A	HOH640
A	HOH729
A	HOH732
A	HOH733

---

site_id	BC5
Number of Residues	6
Details	BINDING SITE FOR RESIDUE GOL A 414

Chain	Residue
A	ASP1
A	ASN11
A	GLU117
A	ILE120
A	HOH556
A	HOH576

---

site_id	BC6
Number of Residues	4
Details	BINDING SITE FOR RESIDUE GOL A 415

Chain	Residue
A	HOH557
A	HOH565
A	HOH615
A	HOH756

---

site_id	BC7
Number of Residues	7
Details	BINDING SITE FOR RESIDUE GOL A 416

Chain	Residue
A	TYR12
A	ASN13
A	ARG42
A	GLU117
A	HOH556
A	HOH755
A	HOH770

---

Functional Information from PROSITE/UniProt

site_id	PS00132
Number of Residues	23
Details	CARBOXYPEPT_ZN_1 Zinc carboxypeptidases, zinc-binding region 1 signature. PeVlYtaLhHArEhLTVemalyT

Chain	Residue	Details
A	PRO60-THR82

---

site_id	PS00133
Number of Residues	11
Details	CARBOXYPEPT_ZN_2 Zinc carboxypeptidases, zinc-binding region 2 signature. HTYSELIlYPY

Chain	Residue	Details
A	HIS204-TYR214

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	304
Details	Domain: {"description":"Peptidase M14","evidences":[{"source":"PROSITE-ProRule","id":"PRU01379","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	1
Details	Active site: {"description":"Proton donor/acceptor","evidences":[{"source":"PROSITE-ProRule","id":"PRU01379","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"1521526","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	3
Details	Binding site: {"evidences":[{"source":"PROSITE-ProRule","id":"PRU01379","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"1521526","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---