4IBW

Human p53 core domain with hot spot mutation R273H and second-site suppressor mutation T284R in sequence-specific complex with DNA

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000976	molecular_function	transcription cis-regulatory region binding
A	0003677	molecular_function	DNA binding
A	0003700	molecular_function	DNA-binding transcription factor activity
A	0005634	cellular_component	nucleus
A	0006355	biological_process	regulation of DNA-templated transcription
A	0006915	biological_process	apoptotic process

Functional Information from PDB Data

site_id	AC1
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN A 301

Chain	Residue
A	CYS176
A	HIS179
A	CYS238
A	CYS242

---

site_id	AC2
Number of Residues	4
Details	BINDING SITE FOR RESIDUE EDO A 302

Chain	Residue
A	SER95
A	THR231
A	HIS233
A	HOH659

---

site_id	AC3
Number of Residues	9
Details	BINDING SITE FOR RESIDUE EDO A 303

Chain	Residue
A	ASP148
A	ASP208
A	ASN210
A	EDO306
A	HOH465
A	HOH559
A	HOH658
A	HOH683
A	ARG110

---

site_id	AC4
Number of Residues	5
Details	BINDING SITE FOR RESIDUE EDO A 304

Chain	Residue
A	GLU224
A	VAL225
A	HOH518
A	HOH633
A	HOH673

---

site_id	AC5
Number of Residues	5
Details	BINDING SITE FOR RESIDUE EDO A 305

Chain	Residue
A	ARG110
A	LEU111
A	TRP146
A	HOH555
A	HOH607

---

site_id	AC6
Number of Residues	10
Details	BINDING SITE FOR RESIDUE EDO A 306

Chain	Residue
A	ARG110
A	TRP146
A	VAL147
A	ASP148
A	ASP228
A	EDO303
A	HOH456
A	HOH575
A	HOH658
A	HOH686

---

site_id	AC7
Number of Residues	6
Details	BINDING SITE FOR RESIDUE EDO A 307

Chain	Residue
A	LEU114
A	SER166
A	MET169
A	HIS233
A	HOH498
A	HOH536

---

site_id	AC8
Number of Residues	10
Details	BINDING SITE FOR RESIDUE EDO A 308

Chain	Residue
A	PHE113
A	LEU114
A	HIS115
A	TYR126
A	PRO128
A	ARG282
A	HOH466
A	HOH477
A	HOH532
A	HOH622

---

site_id	AC9
Number of Residues	8
Details	BINDING SITE FOR RESIDUE EDO A 309

Chain	Residue
A	LYS132
A	SER240
A	ARG248
A	PRO250
A	GLU271
A	VAL272
A	HIS273
A	HOH507

---

site_id	BC1
Number of Residues	4
Details	BINDING SITE FOR RESIDUE EDO A 310

Chain	Residue
A	ASP186
A	ARG196
A	ASN235
A	HOH669

---

site_id	BC2
Number of Residues	7
Details	BINDING SITE FOR RESIDUE EDO A 311

Chain	Residue
A	ALA138
A	LYS139
A	THR140
A	GLN167
A	GLU198
A	ASN235
A	HOH655

---

site_id	BC3
Number of Residues	4
Details	BINDING SITE FOR RESIDUE EDO A 312

Chain	Residue
A	CYS229
A	THR231
A	HOH470
A	HOH548

---

site_id	BC4
Number of Residues	8
Details	BINDING SITE FOR RESIDUE EDO A 313

Chain	Residue
A	SER94
A	SER95
A	SER96
A	ASN200
A	LEU201
A	EDO315
A	HOH594
A	HOH679

---

site_id	BC5
Number of Residues	7
Details	BINDING SITE FOR RESIDUE EDO A 314

Chain	Residue
A	LYS120
A	ARG280
A	ARG283
A	HOH464
A	HOH547
B	DG3
B	HOH227

---

site_id	BC6
Number of Residues	7
Details	BINDING SITE FOR RESIDUE EDO A 315

Chain	Residue
A	SER95
A	GLU221
A	THR230
A	THR231
A	EDO313
A	HOH492
A	HOH634

---

site_id	BC7
Number of Residues	3
Details	BINDING SITE FOR RESIDUE EDO A 316

Chain	Residue
A	GLU286
A	GLU287
A	ARG283

---

site_id	BC8
Number of Residues	5
Details	BINDING SITE FOR RESIDUE EDO B 101

Chain	Residue
A	ARG248
B	DT7
B	DG8
B	EDO102
B	HOH241

---

site_id	BC9
Number of Residues	7
Details	BINDING SITE FOR RESIDUE EDO B 102

Chain	Residue
A	SER241
A	ARG248
B	DC9
B	EDO101
B	HOH213
B	HOH219
B	HOH223

---

Functional Information from PROSITE/UniProt

site_id	PS00348
Number of Residues	13
Details	P53 p53 family signature. MCNSSCMGGMNRR

Chain	Residue	Details
A	MET237-ARG249

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	190
Details	DNA_BIND: DNA_BIND => ECO:0000269|PubMed:16793544, ECO:0000269|PubMed:18996393, ECO:0000269|PubMed:20364130

Chain	Residue	Details
A	THR102-LYS292

---

site_id	SWS_FT_FI2
Number of Residues	4
Details	BINDING: BINDING => ECO:0000269|PubMed:14534297, ECO:0000269|PubMed:16793544, ECO:0000269|PubMed:17015838, ECO:0000269|PubMed:18650397, ECO:0000269|PubMed:19515728, ECO:0000269|PubMed:20142040, ECO:0000269|PubMed:20364130

Chain	Residue	Details
A	CYS176
A	HIS179
A	CYS238
A	CYS242

---

site_id	SWS_FT_FI3
Number of Residues	1
Details	SITE: Interaction with DNA => ECO:0000269|PubMed:16793544, ECO:0000269|PubMed:18996393, ECO:0000269|PubMed:20364130

Chain	Residue	Details
A	LYS120

---

site_id	SWS_FT_FI4
Number of Residues	1
Details	MOD_RES: N6-acetyllysine; by KAT6A => ECO:0000269|PubMed:23431171

Chain	Residue	Details
A	LYS120

---

site_id	SWS_FT_FI5
Number of Residues	2
Details	MOD_RES: Phosphoserine; by AURKB => ECO:0000269|PubMed:20959462

Chain	Residue	Details
A	SER183
A	SER269

---

site_id	SWS_FT_FI6
Number of Residues	1
Details	MOD_RES: Phosphothreonine; by AURKB => ECO:0000269|PubMed:20959462

Chain	Residue	Details
A	ARG284

---

site_id	SWS_FT_FI7
Number of Residues	3
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:19536131

Chain	Residue	Details
A	LYS291
A	LYS292

---