4IAV

G215S, A251G, T257A, D260G, T262D mutant of carboxypeptidase T from Thermoactinomyces vulgaris with N-Sulfamoyl-L-phenylalanine

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004181	molecular_function	metallocarboxypeptidase activity
A	0006508	biological_process	proteolysis
A	0008270	molecular_function	zinc ion binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN A 401

Chain	Residue
A	HIS69
A	GLU72
A	HIS204
A	CXA402

---

site_id	AC2
Number of Residues	14
Details	BINDING SITE FOR RESIDUE CXA A 402

Chain	Residue
A	HIS204
A	THR205
A	LEU211
A	GLY251
A	TYR255
A	ALA257
A	GLU277
A	ZN401
A	HOH660
A	HIS69
A	GLU72
A	ARG129
A	ASN146
A	ARG147

---

site_id	AC3
Number of Residues	6
Details	BINDING SITE FOR RESIDUE CA A 403

Chain	Residue
A	SER7
A	TYR9
A	GLU14
A	HOH570
A	HOH676
A	HOH755

---

site_id	AC4
Number of Residues	7
Details	BINDING SITE FOR RESIDUE GOL A 404

Chain	Residue
A	TYR125
A	SER223
A	TYR288
A	HOH595
A	HOH625
A	HOH711
A	HOH768

---

site_id	AC5
Number of Residues	5
Details	BINDING SITE FOR RESIDUE CA A 405

Chain	Residue
A	SER50
A	ASP51
A	GLU57
A	GLU59
A	HOH587

---

site_id	AC6
Number of Residues	6
Details	BINDING SITE FOR RESIDUE CA A 406

Chain	Residue
A	ASP56
A	GLU57
A	GLU61
A	GLU104
A	HOH685
A	HOH686

---

site_id	AC7
Number of Residues	6
Details	BINDING SITE FOR RESIDUE SO4 A 407

Chain	Residue
A	ARG183
A	ASN187
A	TRP264
A	GLN268
A	HOH607
A	HOH654

---

site_id	AC8
Number of Residues	6
Details	BINDING SITE FOR RESIDUE SO4 A 408

Chain	Residue
A	VAL16
A	ASN20
A	ASN25
A	LYS32
A	TRP45
A	HOH548

---

site_id	AC9
Number of Residues	7
Details	BINDING SITE FOR RESIDUE GOL A 409

Chain	Residue
A	GLY193
A	LYS194
A	GLN195
A	HIS269
A	HOH596
A	HOH637
A	HOH751

---

site_id	BC1
Number of Residues	6
Details	BINDING SITE FOR RESIDUE GOL A 410

Chain	Residue
A	LYS126
A	SER127
A	TRP128
A	LYS152
A	GLU166
A	HOH635

---

site_id	BC2
Number of Residues	6
Details	BINDING SITE FOR RESIDUE GOL A 411

Chain	Residue
A	SER34
A	GLY36
A	LYS37
A	GLU43
A	HOH593
A	HOH790

---

site_id	BC3
Number of Residues	3
Details	BINDING SITE FOR RESIDUE GOL A 412

Chain	Residue
A	HIS10
A	LYS18
A	HOH682

---

site_id	BC4
Number of Residues	3
Details	BINDING SITE FOR RESIDUE GOL A 413

Chain	Residue
A	LYS48
A	TYR106
A	HOH575

---

site_id	BC5
Number of Residues	8
Details	BINDING SITE FOR RESIDUE GOL A 414

Chain	Residue
A	GLY137
A	SER138
A	SER139
A	TYR140
A	ARG171
A	SER172
A	HOH544
A	HOH602

---

site_id	BC6
Number of Residues	5
Details	BINDING SITE FOR RESIDUE GOL A 415

Chain	Residue
A	PRO3
A	SER4
A	TYR5
A	HOH681
A	HOH743

---

site_id	BC7
Number of Residues	3
Details	BINDING SITE FOR RESIDUE GOL A 416

Chain	Residue
A	GLU303
A	HOH599
A	HOH706

---

site_id	BC8
Number of Residues	7
Details	BINDING SITE FOR RESIDUE CA A 417

Chain	Residue
A	HOH753
A	HOH754
A	HOH762
A	TYR5
A	ASP291
A	GLU292
A	HOH591

---

site_id	BC9
Number of Residues	2
Details	BINDING SITE FOR RESIDUE SO4 A 418

Chain	Residue
A	TYR206
A	SER207

---

site_id	CC1
Number of Residues	4
Details	BINDING SITE FOR RESIDUE GOL A 419

Chain	Residue
A	SER139
A	TYR140
A	HOH787
A	HOH788

---

site_id	CC2
Number of Residues	6
Details	BINDING SITE FOR RESIDUE CA A 420

Chain	Residue
A	ASP51
A	GLU57
A	GLU59
A	ASN101
A	HOH756
A	HOH757

---

Functional Information from PROSITE/UniProt

site_id	PS00132
Number of Residues	23
Details	CARBOXYPEPT_ZN_1 Zinc carboxypeptidases, zinc-binding region 1 signature. PeVlYtaLhHArEhLTVemalyT

Chain	Residue	Details
A	PRO60-THR82

---

site_id	PS00133
Number of Residues	11
Details	CARBOXYPEPT_ZN_2 Zinc carboxypeptidases, zinc-binding region 2 signature. HTYSELIlYPY

Chain	Residue	Details
A	HIS204-TYR214

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	304
Details	Domain: {"description":"Peptidase M14","evidences":[{"source":"PROSITE-ProRule","id":"PRU01379","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	1
Details	Active site: {"description":"Proton donor/acceptor","evidences":[{"source":"PROSITE-ProRule","id":"PRU01379","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"1521526","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	3
Details	Binding site: {"evidences":[{"source":"PROSITE-ProRule","id":"PRU01379","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"1521526","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---