Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0004146 | molecular_function | dihydrofolate reductase activity |
| A | 0006730 | biological_process | one-carbon metabolic process |
| A | 0016491 | molecular_function | oxidoreductase activity |
| A | 0046654 | biological_process | tetrahydrofolate biosynthetic process |
| A | 0050661 | molecular_function | NADP binding |
| B | 0004146 | molecular_function | dihydrofolate reductase activity |
| B | 0006730 | biological_process | one-carbon metabolic process |
| B | 0016491 | molecular_function | oxidoreductase activity |
| B | 0046654 | biological_process | tetrahydrofolate biosynthetic process |
| B | 0050661 | molecular_function | NADP binding |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 35 |
| Details | BINDING SITE FOR RESIDUE NDP A 201 |
| Chain | Residue |
| A | VAL10 |
| A | ARG56 |
| A | LYS57 |
| A | THR58 |
| A | LEU77 |
| A | SER78 |
| A | ARG79 |
| A | SER94 |
| A | ILE112 |
| A | GLY114 |
| A | ALA115 |
| A | ALA11 |
| A | GLU116 |
| A | ILE117 |
| A | TYR118 |
| A | GLU120 |
| A | GLU174 |
| A | 18H202 |
| A | HOH331 |
| A | HOH340 |
| A | HOH350 |
| A | HOH369 |
| A | ILE19 |
| A | HOH394 |
| A | HOH397 |
| A | HOH406 |
| A | HOH411 |
| A | HOH425 |
| A | HOH435 |
| A | GLY20 |
| A | GLY23 |
| A | LYS24 |
| A | MET25 |
| A | LYS31 |
| A | GLY55 |
| site_id | AC2 |
| Number of Residues | 14 |
| Details | BINDING SITE FOR RESIDUE 18H A 202 |
| Chain | Residue |
| A | ILE9 |
| A | VAL10 |
| A | ALA11 |
| A | MET25 |
| A | GLU32 |
| A | PHE36 |
| A | THR58 |
| A | SER61 |
| A | ILE62 |
| A | PRO63 |
| A | PHE66 |
| A | ILE112 |
| A | TYR118 |
| A | NDP201 |
| site_id | AC3 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE GLY A 203 |
| Chain | Residue |
| A | ASN5 |
| A | ARG108 |
| A | VAL109 |
| A | SER128 |
| A | HIS129 |
| A | PHE167 |
| site_id | AC4 |
| Number of Residues | 8 |
| Details | BINDING SITE FOR RESIDUE GOL A 204 |
| Chain | Residue |
| A | SER94 |
| A | SER95 |
| A | SER98 |
| A | VAL172 |
| A | HOH309 |
| A | HOH311 |
| A | HOH313 |
| A | HOH360 |
| site_id | AC5 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE GOL A 205 |
| Chain | Residue |
| A | GLU60 |
| A | GLN64 |
| A | ARG67 |
| A | HOH351 |
| site_id | AC6 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE GOL A 206 |
| Chain | Residue |
| A | SER80 |
| A | TYR81 |
| A | GLU82 |
| A | GLU84 |
| A | HIS92 |
| A | HOH365 |
| A | HOH426 |
| site_id | AC7 |
| Number of Residues | 35 |
| Details | BINDING SITE FOR RESIDUE NDP B 201 |
| Chain | Residue |
| B | HOH345 |
| B | HOH350 |
| B | HOH372 |
| B | HOH388 |
| B | HOH402 |
| B | HOH424 |
| B | HOH427 |
| B | HOH430 |
| B | HOH444 |
| B | HOH471 |
| B | VAL10 |
| B | ALA11 |
| B | ILE19 |
| B | GLY20 |
| B | GLY23 |
| B | LYS24 |
| B | MET25 |
| B | GLY55 |
| B | ARG56 |
| B | LYS57 |
| B | THR58 |
| B | LEU77 |
| B | SER78 |
| B | ARG79 |
| B | SER94 |
| B | SER95 |
| B | ILE96 |
| B | ILE112 |
| B | GLY114 |
| B | ALA115 |
| B | GLU116 |
| B | ILE117 |
| B | TYR118 |
| B | GLU120 |
| B | 18H202 |
| site_id | AC8 |
| Number of Residues | 11 |
| Details | BINDING SITE FOR RESIDUE 18H B 202 |
| Chain | Residue |
| B | ILE9 |
| B | VAL10 |
| B | ALA11 |
| B | MET25 |
| B | GLU32 |
| B | PHE36 |
| B | SER61 |
| B | PRO63 |
| B | ILE112 |
| B | TYR118 |
| B | NDP201 |
| site_id | AC9 |
| Number of Residues | 8 |
| Details | BINDING SITE FOR RESIDUE GOL B 203 |
| Chain | Residue |
| A | LYS3 |
| A | HOH455 |
| B | LEU102 |
| B | SER104 |
| B | ASP105 |
| B | HOH304 |
| B | HOH335 |
| B | HOH482 |
Functional Information from PROSITE/UniProt
| site_id | PS00075 |
| Number of Residues | 23 |
| Details | DHFR_1 Dihydrofolate reductase (DHFR) domain signature. GIGykgkMPWrlrk.EiryFkdvT |
| Chain | Residue | Details |
| A | GLY18-THR40 | |
Functional Information from SwissProt/UniProt
| Chain | Residue | Details |
| A | ALA11 | |
| B | GLY113 | |
| A | GLY18 | |
| A | ARG56 | |
| A | SER78 | |
| A | GLY113 | |
| B | ALA11 | |
| B | GLY18 | |
| B | ARG56 | |
| B | SER78 | |
| Chain | Residue | Details |
| A | GLU32 | |
| A | ILE112 | |
| A | TYR118 | |
| B | GLU32 | |
| B | ILE112 | |
| B | TYR118 | |
| Chain | Residue | Details |
| A | ARG72 | |
| B | ARG72 | |
