4GY7

Crystallographic structure analysis of urease from Jack bean (Canavalia ensiformis) at 1.49 A Resolution

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0009039	molecular_function	urease activity
A	0016151	molecular_function	nickel cation binding
A	0016787	molecular_function	hydrolase activity
A	0016810	molecular_function	hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds
A	0035550	cellular_component	urease complex
A	0035821	biological_process	modulation of process of another organism
A	0043419	biological_process	urea catabolic process
A	0046872	molecular_function	metal ion binding
A	0090729	molecular_function	toxin activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	7
Details	BINDING SITE FOR RESIDUE NI A 901

Chain	Residue
A	KCX490
A	HIS492
A	HIS519
A	HIS545
A	GLY550
A	NI902
A	PO4903

---

site_id	AC2
Number of Residues	6
Details	BINDING SITE FOR RESIDUE NI A 902

Chain	Residue
A	KCX490
A	ASP633
A	NI901
A	PO4903
A	HIS407
A	HIS409

---

site_id	AC3
Number of Residues	13
Details	BINDING SITE FOR RESIDUE PO4 A 903

Chain	Residue
A	HIS407
A	HIS409
A	ALA440
A	KCX490
A	HIS492
A	HIS519
A	HIS545
A	GLY550
A	ASP633
A	ALA636
A	MET637
A	NI901
A	NI902

---

site_id	AC4
Number of Residues	7
Details	BINDING SITE FOR RESIDUE EDO A 904

Chain	Residue
A	ASN193
A	ILE194
A	VAL200
A	VAL210
A	THR211
A	HOH1528
A	HOH1757

---

site_id	AC5
Number of Residues	8
Details	BINDING SITE FOR RESIDUE EDO A 905

Chain	Residue
A	PRO5
A	TYR417
A	ACN916
A	HOH1122
A	HOH1322
A	HOH1531
A	HOH1543
A	HOH1810

---

site_id	AC6
Number of Residues	7
Details	BINDING SITE FOR RESIDUE EDO A 906

Chain	Residue
A	GLU9
A	GLN414
A	MET746
A	ARG747
A	HOH1322
A	HOH1533
A	HOH1744

---

site_id	AC7
Number of Residues	6
Details	BINDING SITE FOR RESIDUE EDO A 907

Chain	Residue
A	GLY538
A	THR540
A	ARG791
A	LYS792
A	LEU793
A	HOH1727

---

site_id	AC8
Number of Residues	6
Details	BINDING SITE FOR RESIDUE EDO A 908

Chain	Residue
A	PRO178
A	TYR179
A	GLY248
A	EDO912
A	HOH1741
A	HOH1750

---

site_id	AC9
Number of Residues	7
Details	BINDING SITE FOR RESIDUE EDO A 909

Chain	Residue
A	ALA196
A	LYS282
A	VAL314
A	LYS319
A	HOH1413
A	HOH1756
A	HOH1809

---

site_id	BC1
Number of Residues	7
Details	BINDING SITE FOR RESIDUE EDO A 910

Chain	Residue
A	VAL118
A	PRO119
A	SER120
A	SER258
A	HOH1305
A	HOH1638
A	HOH1861

---

site_id	BC2
Number of Residues	6
Details	BINDING SITE FOR RESIDUE EDO A 911

Chain	Residue
A	ASN225
A	KCX247
A	PRO499
A	ASP503
A	HOH1170
A	HOH1229

---

site_id	BC3
Number of Residues	4
Details	BINDING SITE FOR RESIDUE EDO A 912

Chain	Residue
A	TYR179
A	GLU530
A	HIS531
A	EDO908

---

site_id	BC4
Number of Residues	7
Details	BINDING SITE FOR RESIDUE EDO A 913

Chain	Residue
A	GLN692
A	TYR693
A	ALA786
A	HOH1044
A	HOH1199
A	HOH1352
A	HOH1414

---

site_id	BC5
Number of Residues	3
Details	BINDING SITE FOR RESIDUE ACN A 914

Chain	Residue
A	SER813
A	THR815
A	HOH1541

---

site_id	BC6
Number of Residues	6
Details	BINDING SITE FOR RESIDUE ACN A 915

Chain	Residue
A	HOH1755
A	GLN166
A	GLY317
A	HIS593
A	PHE605
A	HOH1251

---

site_id	BC7
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ACN A 916

Chain	Residue
A	EDO905
A	HOH1543
A	HOH1744
A	HOH1795

---

site_id	BC8
Number of Residues	5
Details	BINDING SITE FOR RESIDUE ACN A 917

Chain	Residue
A	GLN82
A	ARG439
A	VAL591
A	ILE738
A	THR740

---

site_id	BC9
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ACN A 918

Chain	Residue
A	GLN166
A	ALA199
A	GLU601
A	ASP602

---

site_id	CC1
Number of Residues	6
Details	BINDING SITE FOR RESIDUE BME A 919

Chain	Residue
A	GLY316
A	GLY317
A	ASP494
A	CME592
A	HOH1646
A	HOH1753

---

site_id	CC2
Number of Residues	7
Details	BINDING SITE FOR RESIDUE EDO A 920

Chain	Residue
A	TYR417
A	LEU460
A	PRO461
A	TRP728
A	THR752
A	HOH1005
A	HOH1795

---

site_id	CC3
Number of Residues	4
Details	BINDING SITE FOR RESIDUE EDO A 921

Chain	Residue
A	ARG26
A	GLY27
A	ASP49
A	HOH1599

---

site_id	CC4
Number of Residues	9
Details	BINDING SITE FOR RESIDUE EDO A 922

Chain	Residue
A	GLY362
A	LEU702
A	GLY723
A	ALA759
A	LEU760
A	ARG783
A	HOH1264
A	HOH1758
A	HOH1761

---

site_id	CC5
Number of Residues	8
Details	BINDING SITE FOR RESIDUE PO4 A 923

Chain	Residue
A	HIS511
A	HIS512
A	LYS767
A	ALA768
A	ASP771
A	HOH1035
A	HOH1466
A	HOH1804

---

site_id	CC6
Number of Residues	3
Details	BINDING SITE FOR RESIDUE NA A 924

Chain	Residue
A	PRO164
A	PRO811
A	HOH1319

---

site_id	CC7
Number of Residues	3
Details	BINDING SITE FOR RESIDUE NA A 925

Chain	Residue
A	ALA828
A	THR829
A	HOH1552

---

Functional Information from PROSITE/UniProt

site_id	PS00145
Number of Residues	17
Details	UREASE_2 Urease active site. MVCHHLdreIpeDLaFA

Chain	Residue	Details
A	MET590-ALA606

---

site_id	PS01120
Number of Residues	14
Details	UREASE_1 Urease nickel ligands signature. TAGAIDcHVHyicP

Chain	Residue	Details
A	THR400-PRO413

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1
Details	Active site: {"description":"Proton donor","evidences":[{"evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	5
Details	Binding site: {"evidences":[{"source":"PubMed","id":"20471401","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"3LA4","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4GOA","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4GY7","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4H9M","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	1
Details	Binding site: {"description":"via carbamate group","evidences":[{"source":"PubMed","id":"20471401","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"3LA4","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4GOA","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4GY7","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4H9M","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	1
Details	Binding site: {"evidences":[{"evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	1
Details	Modified residue: {"description":"N6-carboxylysine","evidences":[{"source":"PubMed","id":"20471401","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---