Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

4GP4

Structure of Recombinant Cytochrome ba3 Oxidase mutant Y133F from Thermus thermophilus

Replaces: 4F05

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004129	molecular_function	cytochrome-c oxidase activity
A	0005886	cellular_component	plasma membrane
A	0006119	biological_process	oxidative phosphorylation
A	0006811	biological_process	monoatomic ion transport
A	0009060	biological_process	aerobic respiration
A	0016020	cellular_component	membrane
A	0020037	molecular_function	heme binding
A	0022904	biological_process	respiratory electron transport chain
A	0046872	molecular_function	metal ion binding
A	1902600	biological_process	proton transmembrane transport
B	0004129	molecular_function	cytochrome-c oxidase activity
B	0005507	molecular_function	copper ion binding
B	0005886	cellular_component	plasma membrane
B	0016020	cellular_component	membrane
B	0022904	biological_process	respiratory electron transport chain
B	0046872	molecular_function	metal ion binding
B	1902600	biological_process	proton transmembrane transport
C	0004129	molecular_function	cytochrome-c oxidase activity
C	0005886	cellular_component	plasma membrane
C	0006811	biological_process	monoatomic ion transport
C	0022904	biological_process	respiratory electron transport chain
C	1902600	biological_process	proton transmembrane transport

Functional Information from PDB Data

site_id	AC1
Number of Residues	4
Details	BINDING SITE FOR RESIDUE CU A 601

Chain	Residue
A	HIS233
A	HIS282
A	HIS283
A	PER604

site_id	AC2
Number of Residues	22
Details	BINDING SITE FOR RESIDUE HEM A 602

Chain	Residue
A	ASN76
A	ALA77
A	LEU132
A	PHE133
A	PHE385
A	HIS386
A	VAL389
A	ALA390
A	THR394
A	MET432
A	MET435
A	ARG449
A	ARG450
A	ALA451
A	LEU477
A	HOH719
A	HOH725
A	GLY39
A	GLN42
A	TYR46
A	TYR65
A	HIS72

site_id	AC3
Number of Residues	32
Details	BINDING SITE FOR RESIDUE HAS A 603

Chain	Residue
A	PHE133
A	TRP229
A	VAL236
A	TYR237
A	TRP239
A	LEU240
A	HIS282
A	HIS283
A	SER309
A	ALA313
A	ALA317
A	LEU320
A	TRP335
A	VAL350
A	LEU353
A	PHE356
A	GLY360
A	GLY363
A	ILE364
A	ASN366
A	ALA367
A	ASP372
A	HIS376
A	HIS384
A	PHE385
A	GLN388
A	VAL389
A	ARG449
A	PER604
A	HOH701
A	HOH770
A	HOH771

site_id	AC4
Number of Residues	5
Details	BINDING SITE FOR RESIDUE PER A 604

Chain	Residue
A	HIS233
A	VAL236
A	HIS283
A	CU601
A	HAS603

site_id	AC5
Number of Residues	10
Details	BINDING SITE FOR RESIDUE OLC A 605

Chain	Residue
A	PRO358
A	MET433
A	HIS440
A	OLC606
B	OLC203
C	PHE22
C	GLY25
C	ALA28
C	VAL29
C	ALA32

site_id	AC6
Number of Residues	5
Details	BINDING SITE FOR RESIDUE OLC A 606

Chain	Residue
A	LEU354
A	ILE357
A	OLC605
B	OLC203
C	THR18

site_id	AC7
Number of Residues	9
Details	BINDING SITE FOR RESIDUE OLC A 607

Chain	Residue
A	LEU105
A	VAL158
A	TYR161
A	LEU427
A	ILE475
A	VAL476
A	VAL479
A	OLC615
A	OLC616

site_id	AC8
Number of Residues	9
Details	BINDING SITE FOR RESIDUE OLC A 608

Chain	Residue
A	ILE115
A	PHE213
A	LEU215
A	TRP341
A	LEU422
A	TRP426
A	OLC610
A	OLC614
A	OLC616

site_id	AC9
Number of Residues	6
Details	BINDING SITE FOR RESIDUE OLC A 609

Chain	Residue
A	ILE297
B	ALA42
B	HOH303
A	PRO292
A	THR293
A	MET296

site_id	BC1
Number of Residues	6
Details	BINDING SITE FOR RESIDUE OLC A 610

Chain	Residue
A	TRP143
A	SER212
A	PHE213
A	LEU430
A	OLC608
A	OLC617

site_id	BC2
Number of Residues	2
Details	BINDING SITE FOR RESIDUE OLC A 611

Chain	Residue
A	TRP111
A	OLC614

site_id	BC3
Number of Residues	5
Details	BINDING SITE FOR RESIDUE OLC A 612

Chain	Residue
A	TYR161
A	LEU164
A	ASP165
A	ARG168
A	OLC613

site_id	BC4
Number of Residues	4
Details	BINDING SITE FOR RESIDUE OLC A 613

Chain	Residue
A	TRP167
A	ARG168
A	GLY528
A	OLC612

site_id	BC5
Number of Residues	8
Details	BINDING SITE FOR RESIDUE OLC A 614

Chain	Residue
A	GLY104
A	LEU108
A	TRP111
A	VAL468
A	OLC608
A	OLC611
A	OLC615
A	OLC617

site_id	BC6
Number of Residues	5
Details	BINDING SITE FOR RESIDUE OLC A 615

Chain	Residue
A	LEU105
A	VAL151
A	OLC607
A	OLC614
A	OLC616

site_id	BC7
Number of Residues	8
Details	BINDING SITE FOR RESIDUE OLC A 616

Chain	Residue
A	VAL216
A	ASP415
A	ARG419
A	ALA423
A	OLC607
A	OLC608
A	OLC615
A	HOH702

site_id	BC8
Number of Residues	5
Details	BINDING SITE FOR RESIDUE OLC A 617

Chain	Residue
A	ARG337
A	TRP341
A	LEU430
A	OLC610
A	OLC614

site_id	BC9
Number of Residues	6
Details	BINDING SITE FOR RESIDUE CUA B 201

Chain	Residue
B	HIS114
B	CYS149
B	GLN151
B	CYS153
B	HIS157
B	MET160

site_id	CC1
Number of Residues	4
Details	BINDING SITE FOR RESIDUE OLC B 202

Chain	Residue
B	PHE21
B	LEU32
B	TYR35
C	PHE31

site_id	CC2
Number of Residues	7
Details	BINDING SITE FOR RESIDUE OLC B 203

Chain	Residue
A	LEU444
A	OLC605
A	OLC606
B	ARG141
B	GLU144
B	TYR145
C	ARG33

site_id	CC3
Number of Residues	5
Details	BINDING SITE FOR RESIDUE OLC B 204

Chain	Residue
B	ALA13
B	TYR14
B	GLY17
B	PHE21
B	TYR35

Functional Information from PROSITE/UniProt

site_id	PS00077
Number of Residues	55
Details	COX1_CUB Heme-copper oxidase catalytic subunit, copper B binding region signature. WWTGHPiVyfwllpayaiiytilpkqaggklvsdpmarlafllflllstpvgf..HH

Chain	Residue	Details
A	TRP229-HIS283

site_id	PS00078
Number of Residues	49
Details	COX2 CO II and nitrous oxide reductase dinuclear copper centers signature. ViHgfhvegtninvevlpgevstvrytfkrpgeyrii......CnqyCglgHqnM

Chain	Residue	Details
B	VAL112-MET160

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	260
Details	Transmembrane: {"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

site_id	SWS_FT_FI2
Number of Residues	3
Details	Binding site: {"description":"axial binding residue","evidences":[{"evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

site_id	SWS_FT_FI3
Number of Residues	6
Details	Binding site: {}

Chain

Residue

Details

site_id	SWS_FT_FI4
Number of Residues	2
Details	Binding site: {"evidences":[{"evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

site_id	SWS_FT_FI5
Number of Residues	2
Details	Cross-link: {"description":"1'-histidyl-3'-tyrosine (His-Tyr)"}

Chain

Residue

Details

site_id	SWS_FT_FI6
Number of Residues	64
Details	Transmembrane: {"description":"Helical"}

Chain

Residue

Details

site_id	SWS_FT_FI7
Number of Residues	129
Details	Topological domain: {"description":"Periplasmic"}

Chain

Residue

Details

Catalytic Information from CSA

site_id	MCSA1
Number of Residues	2
Details	M-CSA 735

Chain	Residue	Details
B	PHE86	electron shuttle
B	PHE88	electron shuttle
A	HIS384	electron shuttle
A	PHE385	electron shuttle
A	HIS386	electron shuttle
A	ARG449	electron shuttle
A	ARG450	electron shuttle

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)