4G4E

Crystal structure of the L88A mutant of HslV from Escherichia coli

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000287	molecular_function	magnesium ion binding
A	0003824	molecular_function	catalytic activity
A	0004176	molecular_function	ATP-dependent peptidase activity
A	0004298	molecular_function	threonine-type endopeptidase activity
A	0005515	molecular_function	protein binding
A	0005524	molecular_function	ATP binding
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0005839	cellular_component	proteasome core complex
A	0006508	biological_process	proteolysis
A	0008233	molecular_function	peptidase activity
A	0009376	cellular_component	HslUV protease complex
A	0009408	biological_process	response to heat
A	0016787	molecular_function	hydrolase activity
A	0019904	molecular_function	protein domain specific binding
A	0030163	biological_process	protein catabolic process
A	0030164	biological_process	protein denaturation
A	0034605	biological_process	cellular response to heat
A	0042802	molecular_function	identical protein binding
A	0046872	molecular_function	metal ion binding
A	0051603	biological_process	proteolysis involved in protein catabolic process
B	0000287	molecular_function	magnesium ion binding
B	0003824	molecular_function	catalytic activity
B	0004176	molecular_function	ATP-dependent peptidase activity
B	0004298	molecular_function	threonine-type endopeptidase activity
B	0005515	molecular_function	protein binding
B	0005524	molecular_function	ATP binding
B	0005737	cellular_component	cytoplasm
B	0005829	cellular_component	cytosol
B	0005839	cellular_component	proteasome core complex
B	0006508	biological_process	proteolysis
B	0008233	molecular_function	peptidase activity
B	0009376	cellular_component	HslUV protease complex
B	0009408	biological_process	response to heat
B	0016787	molecular_function	hydrolase activity
B	0019904	molecular_function	protein domain specific binding
B	0030163	biological_process	protein catabolic process
B	0030164	biological_process	protein denaturation
B	0034605	biological_process	cellular response to heat
B	0042802	molecular_function	identical protein binding
B	0046872	molecular_function	metal ion binding
B	0051603	biological_process	proteolysis involved in protein catabolic process
C	0000287	molecular_function	magnesium ion binding
C	0003824	molecular_function	catalytic activity
C	0004176	molecular_function	ATP-dependent peptidase activity
C	0004298	molecular_function	threonine-type endopeptidase activity
C	0005515	molecular_function	protein binding
C	0005524	molecular_function	ATP binding
C	0005737	cellular_component	cytoplasm
C	0005829	cellular_component	cytosol
C	0005839	cellular_component	proteasome core complex
C	0006508	biological_process	proteolysis
C	0008233	molecular_function	peptidase activity
C	0009376	cellular_component	HslUV protease complex
C	0009408	biological_process	response to heat
C	0016787	molecular_function	hydrolase activity
C	0019904	molecular_function	protein domain specific binding
C	0030163	biological_process	protein catabolic process
C	0030164	biological_process	protein denaturation
C	0034605	biological_process	cellular response to heat
C	0042802	molecular_function	identical protein binding
C	0046872	molecular_function	metal ion binding
C	0051603	biological_process	proteolysis involved in protein catabolic process
D	0000287	molecular_function	magnesium ion binding
D	0003824	molecular_function	catalytic activity
D	0004176	molecular_function	ATP-dependent peptidase activity
D	0004298	molecular_function	threonine-type endopeptidase activity
D	0005515	molecular_function	protein binding
D	0005524	molecular_function	ATP binding
D	0005737	cellular_component	cytoplasm
D	0005829	cellular_component	cytosol
D	0005839	cellular_component	proteasome core complex
D	0006508	biological_process	proteolysis
D	0008233	molecular_function	peptidase activity
D	0009376	cellular_component	HslUV protease complex
D	0009408	biological_process	response to heat
D	0016787	molecular_function	hydrolase activity
D	0019904	molecular_function	protein domain specific binding
D	0030163	biological_process	protein catabolic process
D	0030164	biological_process	protein denaturation
D	0034605	biological_process	cellular response to heat
D	0042802	molecular_function	identical protein binding
D	0046872	molecular_function	metal ion binding
D	0051603	biological_process	proteolysis involved in protein catabolic process
E	0000287	molecular_function	magnesium ion binding
E	0003824	molecular_function	catalytic activity
E	0004176	molecular_function	ATP-dependent peptidase activity
E	0004298	molecular_function	threonine-type endopeptidase activity
E	0005515	molecular_function	protein binding
E	0005524	molecular_function	ATP binding
E	0005737	cellular_component	cytoplasm
E	0005829	cellular_component	cytosol
E	0005839	cellular_component	proteasome core complex
E	0006508	biological_process	proteolysis
E	0008233	molecular_function	peptidase activity
E	0009376	cellular_component	HslUV protease complex
E	0009408	biological_process	response to heat
E	0016787	molecular_function	hydrolase activity
E	0019904	molecular_function	protein domain specific binding
E	0030163	biological_process	protein catabolic process
E	0030164	biological_process	protein denaturation
E	0034605	biological_process	cellular response to heat
E	0042802	molecular_function	identical protein binding
E	0046872	molecular_function	metal ion binding
E	0051603	biological_process	proteolysis involved in protein catabolic process
F	0000287	molecular_function	magnesium ion binding
F	0003824	molecular_function	catalytic activity
F	0004176	molecular_function	ATP-dependent peptidase activity
F	0004298	molecular_function	threonine-type endopeptidase activity
F	0005515	molecular_function	protein binding
F	0005524	molecular_function	ATP binding
F	0005737	cellular_component	cytoplasm
F	0005829	cellular_component	cytosol
F	0005839	cellular_component	proteasome core complex
F	0006508	biological_process	proteolysis
F	0008233	molecular_function	peptidase activity
F	0009376	cellular_component	HslUV protease complex
F	0009408	biological_process	response to heat
F	0016787	molecular_function	hydrolase activity
F	0019904	molecular_function	protein domain specific binding
F	0030163	biological_process	protein catabolic process
F	0030164	biological_process	protein denaturation
F	0034605	biological_process	cellular response to heat
F	0042802	molecular_function	identical protein binding
F	0046872	molecular_function	metal ion binding
F	0051603	biological_process	proteolysis involved in protein catabolic process
G	0000287	molecular_function	magnesium ion binding
G	0003824	molecular_function	catalytic activity
G	0004176	molecular_function	ATP-dependent peptidase activity
G	0004298	molecular_function	threonine-type endopeptidase activity
G	0005515	molecular_function	protein binding
G	0005524	molecular_function	ATP binding
G	0005737	cellular_component	cytoplasm
G	0005829	cellular_component	cytosol
G	0005839	cellular_component	proteasome core complex
G	0006508	biological_process	proteolysis
G	0008233	molecular_function	peptidase activity
G	0009376	cellular_component	HslUV protease complex
G	0009408	biological_process	response to heat
G	0016787	molecular_function	hydrolase activity
G	0019904	molecular_function	protein domain specific binding
G	0030163	biological_process	protein catabolic process
G	0030164	biological_process	protein denaturation
G	0034605	biological_process	cellular response to heat
G	0042802	molecular_function	identical protein binding
G	0046872	molecular_function	metal ion binding
G	0051603	biological_process	proteolysis involved in protein catabolic process
H	0000287	molecular_function	magnesium ion binding
H	0003824	molecular_function	catalytic activity
H	0004176	molecular_function	ATP-dependent peptidase activity
H	0004298	molecular_function	threonine-type endopeptidase activity
H	0005515	molecular_function	protein binding
H	0005524	molecular_function	ATP binding
H	0005737	cellular_component	cytoplasm
H	0005829	cellular_component	cytosol
H	0005839	cellular_component	proteasome core complex
H	0006508	biological_process	proteolysis
H	0008233	molecular_function	peptidase activity
H	0009376	cellular_component	HslUV protease complex
H	0009408	biological_process	response to heat
H	0016787	molecular_function	hydrolase activity
H	0019904	molecular_function	protein domain specific binding
H	0030163	biological_process	protein catabolic process
H	0030164	biological_process	protein denaturation
H	0034605	biological_process	cellular response to heat
H	0042802	molecular_function	identical protein binding
H	0046872	molecular_function	metal ion binding
H	0051603	biological_process	proteolysis involved in protein catabolic process
I	0000287	molecular_function	magnesium ion binding
I	0003824	molecular_function	catalytic activity
I	0004176	molecular_function	ATP-dependent peptidase activity
I	0004298	molecular_function	threonine-type endopeptidase activity
I	0005515	molecular_function	protein binding
I	0005524	molecular_function	ATP binding
I	0005737	cellular_component	cytoplasm
I	0005829	cellular_component	cytosol
I	0005839	cellular_component	proteasome core complex
I	0006508	biological_process	proteolysis
I	0008233	molecular_function	peptidase activity
I	0009376	cellular_component	HslUV protease complex
I	0009408	biological_process	response to heat
I	0016787	molecular_function	hydrolase activity
I	0019904	molecular_function	protein domain specific binding
I	0030163	biological_process	protein catabolic process
I	0030164	biological_process	protein denaturation
I	0034605	biological_process	cellular response to heat
I	0042802	molecular_function	identical protein binding
I	0046872	molecular_function	metal ion binding
I	0051603	biological_process	proteolysis involved in protein catabolic process
J	0000287	molecular_function	magnesium ion binding
J	0003824	molecular_function	catalytic activity
J	0004176	molecular_function	ATP-dependent peptidase activity
J	0004298	molecular_function	threonine-type endopeptidase activity
J	0005515	molecular_function	protein binding
J	0005524	molecular_function	ATP binding
J	0005737	cellular_component	cytoplasm
J	0005829	cellular_component	cytosol
J	0005839	cellular_component	proteasome core complex
J	0006508	biological_process	proteolysis
J	0008233	molecular_function	peptidase activity
J	0009376	cellular_component	HslUV protease complex
J	0009408	biological_process	response to heat
J	0016787	molecular_function	hydrolase activity
J	0019904	molecular_function	protein domain specific binding
J	0030163	biological_process	protein catabolic process
J	0030164	biological_process	protein denaturation
J	0034605	biological_process	cellular response to heat
J	0042802	molecular_function	identical protein binding
J	0046872	molecular_function	metal ion binding
J	0051603	biological_process	proteolysis involved in protein catabolic process
K	0000287	molecular_function	magnesium ion binding
K	0003824	molecular_function	catalytic activity
K	0004176	molecular_function	ATP-dependent peptidase activity
K	0004298	molecular_function	threonine-type endopeptidase activity
K	0005515	molecular_function	protein binding
K	0005524	molecular_function	ATP binding
K	0005737	cellular_component	cytoplasm
K	0005829	cellular_component	cytosol
K	0005839	cellular_component	proteasome core complex
K	0006508	biological_process	proteolysis
K	0008233	molecular_function	peptidase activity
K	0009376	cellular_component	HslUV protease complex
K	0009408	biological_process	response to heat
K	0016787	molecular_function	hydrolase activity
K	0019904	molecular_function	protein domain specific binding
K	0030163	biological_process	protein catabolic process
K	0030164	biological_process	protein denaturation
K	0034605	biological_process	cellular response to heat
K	0042802	molecular_function	identical protein binding
K	0046872	molecular_function	metal ion binding
K	0051603	biological_process	proteolysis involved in protein catabolic process
L	0000287	molecular_function	magnesium ion binding
L	0003824	molecular_function	catalytic activity
L	0004176	molecular_function	ATP-dependent peptidase activity
L	0004298	molecular_function	threonine-type endopeptidase activity
L	0005515	molecular_function	protein binding
L	0005524	molecular_function	ATP binding
L	0005737	cellular_component	cytoplasm
L	0005829	cellular_component	cytosol
L	0005839	cellular_component	proteasome core complex
L	0006508	biological_process	proteolysis
L	0008233	molecular_function	peptidase activity
L	0009376	cellular_component	HslUV protease complex
L	0009408	biological_process	response to heat
L	0016787	molecular_function	hydrolase activity
L	0019904	molecular_function	protein domain specific binding
L	0030163	biological_process	protein catabolic process
L	0030164	biological_process	protein denaturation
L	0034605	biological_process	cellular response to heat
L	0042802	molecular_function	identical protein binding
L	0046872	molecular_function	metal ion binding
L	0051603	biological_process	proteolysis involved in protein catabolic process

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	12
Details	Active site: {"evidences":[{"source":"HAMAP-Rule","id":"MF_00248","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"9257689","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	36
Details	Binding site: {}

Chain

Residue

Details

---

Catalytic Information from CSA

site_id	MCSA1
Number of Residues	4
Details	M-CSA 678

Chain	Residue	Details
A	THR1	proton acceptor, proton donor
A	LYS33	electrostatic stabiliser
A	GLY45	electrostatic stabiliser
A	SER124	electrostatic stabiliser

---

site_id	MCSA10
Number of Residues	4
Details	M-CSA 678

Chain	Residue	Details
J	THR1	proton acceptor, proton donor
J	LYS33	electrostatic stabiliser
J	GLY45	electrostatic stabiliser
J	SER124	electrostatic stabiliser

---

site_id	MCSA11
Number of Residues	4
Details	M-CSA 678

Chain	Residue	Details
K	THR1	proton acceptor, proton donor
K	LYS33	electrostatic stabiliser
K	GLY45	electrostatic stabiliser
K	SER124	electrostatic stabiliser

---

site_id	MCSA12
Number of Residues	4
Details	M-CSA 678

Chain	Residue	Details
L	THR1	proton acceptor, proton donor
L	LYS33	electrostatic stabiliser
L	GLY45	electrostatic stabiliser
L	SER124	electrostatic stabiliser

---

site_id	MCSA2
Number of Residues	4
Details	M-CSA 678

Chain	Residue	Details
B	THR1	proton acceptor, proton donor
B	LYS33	electrostatic stabiliser
B	GLY45	electrostatic stabiliser
B	SER124	electrostatic stabiliser

---

site_id	MCSA3
Number of Residues	4
Details	M-CSA 678

Chain	Residue	Details
C	THR1	proton acceptor, proton donor
C	LYS33	electrostatic stabiliser
C	GLY45	electrostatic stabiliser
C	SER124	electrostatic stabiliser

---

site_id	MCSA4
Number of Residues	4
Details	M-CSA 678

Chain	Residue	Details
D	THR1	proton acceptor, proton donor
D	LYS33	electrostatic stabiliser
D	GLY45	electrostatic stabiliser
D	SER124	electrostatic stabiliser

---

site_id	MCSA5
Number of Residues	4
Details	M-CSA 678

Chain	Residue	Details
E	THR1	proton acceptor, proton donor
E	LYS33	electrostatic stabiliser
E	GLY45	electrostatic stabiliser
E	SER124	electrostatic stabiliser

---

site_id	MCSA6
Number of Residues	4
Details	M-CSA 678

Chain	Residue	Details
F	THR1	proton acceptor, proton donor
F	LYS33	electrostatic stabiliser
F	GLY45	electrostatic stabiliser
F	SER124	electrostatic stabiliser

---

site_id	MCSA7
Number of Residues	4
Details	M-CSA 678

Chain	Residue	Details
G	THR1	proton acceptor, proton donor
G	LYS33	electrostatic stabiliser
G	GLY45	electrostatic stabiliser
G	SER124	electrostatic stabiliser

---

site_id	MCSA8
Number of Residues	4
Details	M-CSA 678

Chain	Residue	Details
H	THR1	proton acceptor, proton donor
H	LYS33	electrostatic stabiliser
H	GLY45	electrostatic stabiliser
H	SER124	electrostatic stabiliser

---

site_id	MCSA9
Number of Residues	4
Details	M-CSA 678

Chain	Residue	Details
I	THR1	proton acceptor, proton donor
I	LYS33	electrostatic stabiliser
I	GLY45	electrostatic stabiliser
I	SER124	electrostatic stabiliser

---