4FME

EspG-Rab1-Arf6 complex

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004197	molecular_function	cysteine-type endopeptidase activity
B	0003924	molecular_function	GTPase activity
B	0005525	molecular_function	GTP binding
C	0001726	cellular_component	ruffle
C	0001889	biological_process	liver development
C	0003924	molecular_function	GTPase activity
C	0003925	molecular_function	G protein activity
C	0005515	molecular_function	protein binding
C	0005525	molecular_function	GTP binding
C	0005737	cellular_component	cytoplasm
C	0005768	cellular_component	endosome
C	0005769	cellular_component	early endosome
C	0005794	cellular_component	Golgi apparatus
C	0005829	cellular_component	cytosol
C	0005886	cellular_component	plasma membrane
C	0005925	cellular_component	focal adhesion
C	0005938	cellular_component	cell cortex
C	0006886	biological_process	intracellular protein transport
C	0007155	biological_process	cell adhesion
C	0007399	biological_process	nervous system development
C	0010975	biological_process	regulation of neuron projection development
C	0010976	biological_process	positive regulation of neuron projection development
C	0015031	biological_process	protein transport
C	0016020	cellular_component	membrane
C	0016192	biological_process	vesicle-mediated transport
C	0016787	molecular_function	hydrolase activity
C	0019003	molecular_function	GDP binding
C	0030139	cellular_component	endocytic vesicle
C	0030154	biological_process	cell differentiation
C	0030496	cellular_component	midbody
C	0030838	biological_process	positive regulation of actin filament polymerization
C	0030866	biological_process	cortical actin cytoskeleton organization
C	0031527	cellular_component	filopodium membrane
C	0031901	cellular_component	early endosome membrane
C	0031996	molecular_function	thioesterase binding
C	0032154	cellular_component	cleavage furrow
C	0032456	biological_process	endocytic recycling
C	0034394	biological_process	protein localization to cell surface
C	0035020	biological_process	regulation of Rac protein signal transduction
C	0035591	molecular_function	signaling adaptor activity
C	0036010	biological_process	protein localization to endosome
C	0042995	cellular_component	cell projection
C	0048261	biological_process	negative regulation of receptor-mediated endocytosis
C	0048488	biological_process	synaptic vesicle endocytosis
C	0050714	biological_process	positive regulation of protein secretion
C	0051301	biological_process	cell division
C	0051489	biological_process	regulation of filopodium assembly
C	0051549	biological_process	positive regulation of keratinocyte migration
C	0055037	cellular_component	recycling endosome
C	0055038	cellular_component	recycling endosome membrane
C	0060998	biological_process	regulation of dendritic spine development
C	0070062	cellular_component	extracellular exosome
C	0072659	biological_process	protein localization to plasma membrane
C	0090162	biological_process	establishment of epithelial cell polarity
C	0090543	cellular_component	Flemming body
C	0097178	biological_process	ruffle assembly
C	0097284	biological_process	hepatocyte apoptotic process
C	0098793	cellular_component	presynapse
C	0098794	cellular_component	postsynapse
C	0098978	cellular_component	glutamatergic synapse
C	0099562	biological_process	maintenance of postsynaptic density structure
C	0120183	biological_process	positive regulation of focal adhesion disassembly
C	1902217	biological_process	erythrocyte apoptotic process
C	1903078	biological_process	positive regulation of protein localization to plasma membrane
C	1903438	biological_process	positive regulation of mitotic cytokinetic process
C	1905345	biological_process	protein localization to cleavage furrow
C	1905606	biological_process	regulation of presynapse assembly
C	1990090	biological_process	cellular response to nerve growth factor stimulus
C	2000009	biological_process	negative regulation of protein localization to cell surface
C	2000171	biological_process	negative regulation of dendrite development
D	0004197	molecular_function	cysteine-type endopeptidase activity
E	0003924	molecular_function	GTPase activity
E	0005525	molecular_function	GTP binding
F	0001726	cellular_component	ruffle
F	0001889	biological_process	liver development
F	0003924	molecular_function	GTPase activity
F	0003925	molecular_function	G protein activity
F	0005515	molecular_function	protein binding
F	0005525	molecular_function	GTP binding
F	0005737	cellular_component	cytoplasm
F	0005768	cellular_component	endosome
F	0005769	cellular_component	early endosome
F	0005794	cellular_component	Golgi apparatus
F	0005829	cellular_component	cytosol
F	0005886	cellular_component	plasma membrane
F	0005925	cellular_component	focal adhesion
F	0005938	cellular_component	cell cortex
F	0006886	biological_process	intracellular protein transport
F	0007155	biological_process	cell adhesion
F	0007399	biological_process	nervous system development
F	0010975	biological_process	regulation of neuron projection development
F	0010976	biological_process	positive regulation of neuron projection development
F	0015031	biological_process	protein transport
F	0016020	cellular_component	membrane
F	0016192	biological_process	vesicle-mediated transport
F	0016787	molecular_function	hydrolase activity
F	0019003	molecular_function	GDP binding
F	0030139	cellular_component	endocytic vesicle
F	0030154	biological_process	cell differentiation
F	0030496	cellular_component	midbody
F	0030838	biological_process	positive regulation of actin filament polymerization
F	0030866	biological_process	cortical actin cytoskeleton organization
F	0031527	cellular_component	filopodium membrane
F	0031901	cellular_component	early endosome membrane
F	0031996	molecular_function	thioesterase binding
F	0032154	cellular_component	cleavage furrow
F	0032456	biological_process	endocytic recycling
F	0034394	biological_process	protein localization to cell surface
F	0035020	biological_process	regulation of Rac protein signal transduction
F	0035591	molecular_function	signaling adaptor activity
F	0036010	biological_process	protein localization to endosome
F	0042995	cellular_component	cell projection
F	0048261	biological_process	negative regulation of receptor-mediated endocytosis
F	0048488	biological_process	synaptic vesicle endocytosis
F	0050714	biological_process	positive regulation of protein secretion
F	0051301	biological_process	cell division
F	0051489	biological_process	regulation of filopodium assembly
F	0051549	biological_process	positive regulation of keratinocyte migration
F	0055037	cellular_component	recycling endosome
F	0055038	cellular_component	recycling endosome membrane
F	0060998	biological_process	regulation of dendritic spine development
F	0070062	cellular_component	extracellular exosome
F	0072659	biological_process	protein localization to plasma membrane
F	0090162	biological_process	establishment of epithelial cell polarity
F	0090543	cellular_component	Flemming body
F	0097178	biological_process	ruffle assembly
F	0097284	biological_process	hepatocyte apoptotic process
F	0098793	cellular_component	presynapse
F	0098794	cellular_component	postsynapse
F	0098978	cellular_component	glutamatergic synapse
F	0099562	biological_process	maintenance of postsynaptic density structure
F	0120183	biological_process	positive regulation of focal adhesion disassembly
F	1902217	biological_process	erythrocyte apoptotic process
F	1903078	biological_process	positive regulation of protein localization to plasma membrane
F	1903438	biological_process	positive regulation of mitotic cytokinetic process
F	1905345	biological_process	protein localization to cleavage furrow
F	1905606	biological_process	regulation of presynapse assembly
F	1990090	biological_process	cellular response to nerve growth factor stimulus
F	2000009	biological_process	negative regulation of protein localization to cell surface
F	2000171	biological_process	negative regulation of dendrite development

Functional Information from PDB Data

site_id	AC1
Number of Residues	9
Details	BINDING SITE FOR RESIDUE AF3 B 201

Chain	Residue
A	ARG208
A	HOH401
B	SER20
B	GLY21
B	LYS24
B	THR43
B	GDP202
B	MG203
B	HOH301

---

site_id	AC2
Number of Residues	21
Details	BINDING SITE FOR RESIDUE GDP B 202

Chain	Residue
A	ARG208
A	THR239
B	ASP19
B	GLY21
B	VAL22
B	GLY23
B	LYS24
B	SER25
B	CYS26
B	TYR36
B	GLU38
B	ASN124
B	LYS125
B	ASP127
B	LEU128
B	SER154
B	ALA155
B	LYS156
B	AF3201
B	MG203
B	HOH301

---

site_id	AC3
Number of Residues	6
Details	BINDING SITE FOR RESIDUE MG B 203

Chain	Residue
B	SER25
B	THR43
B	ASP66
B	AF3201
B	GDP202
B	HOH301

---

site_id	AC4
Number of Residues	21
Details	BINDING SITE FOR RESIDUE GTP C 201

Chain	Residue
C	LEU21
C	ASP22
C	ALA23
C	ALA24
C	GLY25
C	LYS26
C	THR27
C	THR28
C	THR41
C	PRO43
C	THR44
C	GLY65
C	GLY66
C	ASN122
C	LYS123
C	ASP125
C	LEU126
C	CYS155
C	ALA156
C	THR157
C	MG202

---

site_id	AC5
Number of Residues	3
Details	BINDING SITE FOR RESIDUE MG C 202

Chain	Residue
C	THR27
C	THR44
C	GTP201

---

site_id	AC6
Number of Residues	9
Details	BINDING SITE FOR RESIDUE AF3 E 201

Chain	Residue
D	ARG208
D	HOH401
E	SER20
E	GLY21
E	LYS24
E	THR43
E	GDP202
E	MG203
E	HOH301

---

site_id	AC7
Number of Residues	21
Details	BINDING SITE FOR RESIDUE GDP E 202

Chain	Residue
D	ARG208
D	THR239
E	ASP19
E	GLY21
E	VAL22
E	GLY23
E	LYS24
E	SER25
E	CYS26
E	TYR36
E	GLU38
E	ASN124
E	LYS125
E	ASP127
E	LEU128
E	SER154
E	ALA155
E	LYS156
E	AF3201
E	MG203
E	HOH301

---

site_id	AC8
Number of Residues	6
Details	BINDING SITE FOR RESIDUE MG E 203

Chain	Residue
E	SER25
E	THR43
E	ASP66
E	AF3201
E	GDP202
E	HOH301

---

site_id	AC9
Number of Residues	21
Details	BINDING SITE FOR RESIDUE GTP F 201

Chain	Residue
F	ALA24
F	GLY25
F	LYS26
F	THR27
F	THR28
F	THR41
F	PRO43
F	THR44
F	GLY65
F	GLY66
F	ASN122
F	LYS123
F	ASP125
F	LEU126
F	CYS155
F	ALA156
F	THR157
F	MG202
F	LEU21
F	ASP22
F	ALA23

---

site_id	BC1
Number of Residues	3
Details	BINDING SITE FOR RESIDUE MG F 202

Chain	Residue
F	THR27
F	THR44
F	GTP201

---

Functional Information from PROSITE/UniProt

site_id	PS00675
Number of Residues	14
Details	SIGMA54_INTERACT_1 Sigma-54 interaction domain ATP-binding region A signature. LLLiGDSGVGKscL

Chain	Residue	Details
B	LEU14-LEU27

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	10
Details	BINDING: BINDING => ECO:0000269|PubMed:16099990, ECO:0000269|PubMed:19644450, ECO:0000269|PubMed:21170023, ECO:0000269|PubMed:22939626, ECO:0000269|PubMed:23940353

Chain	Residue	Details
C	ALA23
F	CYS155
C	THR41
C	ASP63
C	ASN122
C	CYS155
F	ALA23
F	THR41
F	ASP63
F	ASN122

---

site_id	SWS_FT_FI2
Number of Residues	2
Details	BINDING: BINDING => ECO:0000269|PubMed:22416225, ECO:0000269|PubMed:22939626, ECO:0000269|PubMed:23588383, ECO:0000269|PubMed:23821544, ECO:0007744|PDB:2FOL, ECO:0007744|PDB:3SFV, ECO:0007744|PDB:3TKL, ECO:0007744|PDB:4FMC, ECO:0007744|PDB:4FME, ECO:0007744|PDB:4IRU, ECO:0007744|PDB:4JVS

Chain	Residue	Details
B	TYR36
E	TYR36

---

site_id	SWS_FT_FI3
Number of Residues	2
Details	BINDING: BINDING => ECO:0000269|PubMed:22416225, ECO:0007744|PDB:3TKL

Chain	Residue	Details
B	ASP66
E	ASP66

---

site_id	SWS_FT_FI4
Number of Residues	2
Details	MOD_RES: (Microbial infection) O-(2-cholinephosphoryl)serine => ECO:0000269|PubMed:21822290, ECO:0000269|PubMed:22158903

Chain	Residue	Details
B	SER79
E	SER79

---

site_id	SWS_FT_FI5
Number of Residues	2
Details	CARBOHYD: (Microbial infection) N-beta-linked (GlcNAc) arginine => ECO:0000269|PubMed:32504010

Chain	Residue	Details
B	ARG72
E	ARG72

---

site_id	SWS_FT_FI6
Number of Residues	6
Details	CARBOHYD: (Microbial infection) N-beta-linked (GlcNAc) arginine => ECO:0000269|PubMed:32504010, ECO:0000269|PubMed:32974215

Chain	Residue	Details
B	ARG74
B	ARG82
B	ARG111
E	ARG74
E	ARG82
E	ARG111

---

site_id	SWS_FT_FI7
Number of Residues	6
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000250|UniProtKB:P51153

Chain	Residue	Details
B	LYS49
B	LYS61
E	LYS49
E	LYS61

---