4FLR

Crystal structure of Amylosucrase double mutant A289P-F290L from Neisseria polysaccharea

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0005576	cellular_component	extracellular region
A	0005975	biological_process	carbohydrate metabolic process
A	0016757	molecular_function	glycosyltransferase activity
A	0047669	molecular_function	amylosucrase activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	10
Details	BINDING SITE FOR RESIDUE TRS A 701

Chain	Residue
A	ASP144
A	HOH875
A	TYR147
A	HIS187
A	PHE250
A	ASP286
A	GLU328
A	ARG509
A	GOL708
A	GOL717

---

site_id	AC2
Number of Residues	1
Details	BINDING SITE FOR RESIDUE GOL A 702

Chain	Residue
A	ASP403

---

site_id	AC3
Number of Residues	5
Details	BINDING SITE FOR RESIDUE GOL A 703

Chain	Residue
A	HIS332
A	PHE399
A	ASP401
A	GLU402
A	ARG415

---

site_id	AC4
Number of Residues	9
Details	BINDING SITE FOR RESIDUE GOL A 704

Chain	Residue
A	GLU328
A	ILE330
A	TYR349
A	TYR388
A	SER391
A	HIS392
A	ASP393
A	GOL717
A	HOH1000

---

site_id	AC5
Number of Residues	10
Details	BINDING SITE FOR RESIDUE GOL A 705

Chain	Residue
A	ARG86
A	GLU87
A	PRO90
A	ARG317
A	PRO321
A	PHE324
A	GOL706
A	HOH835
A	HOH921
A	HOH922

---

site_id	AC6
Number of Residues	7
Details	BINDING SITE FOR RESIDUE GOL A 706

Chain	Residue
A	PRO90
A	ARG317
A	PHE324
A	GLN342
A	GLN346
A	HIS382
A	GOL705

---

site_id	AC7
Number of Residues	6
Details	BINDING SITE FOR RESIDUE GOL A 707

Chain	Residue
A	ASP91
A	LEU94
A	SER95
A	ASP552
A	GLY553
A	HOH964

---

site_id	AC8
Number of Residues	12
Details	BINDING SITE FOR RESIDUE GOL A 708

Chain	Residue
A	TYR104
A	MET133
A	ASP144
A	GLY145
A	TYR147
A	HIS392
A	ASP393
A	ARG509
A	ARG513
A	TRS701
A	HOH875
A	HOH1004

---

site_id	AC9
Number of Residues	6
Details	BINDING SITE FOR RESIDUE GOL A 709

Chain	Residue
A	GLY396
A	TRP397
A	THR398
A	ASP444
A	CYS445
A	ARG446

---

site_id	BC1
Number of Residues	6
Details	BINDING SITE FOR RESIDUE GOL A 710

Chain	Residue
A	ARG226
A	ILE228
A	GLU328
A	ALA329
A	ILE330
A	GOL717

---

site_id	BC2
Number of Residues	7
Details	BINDING SITE FOR RESIDUE GOL A 711

Chain	Residue
A	PHE229
A	GLN232
A	ASN251
A	GLN437
A	ASP506
A	ASP507
A	ARG509

---

site_id	BC3
Number of Residues	6
Details	BINDING SITE FOR RESIDUE GOL A 712

Chain	Residue
A	TYR51
A	ASN54
A	SER260
A	ASN562
A	THR589
A	THR592

---

site_id	BC4
Number of Residues	8
Details	BINDING SITE FOR RESIDUE GOL A 713

Chain	Residue
A	ILE93
A	LEU94
A	SER95
A	GLY480
A	LEU481
A	ARG545
A	PHE551
A	GLY553

---

site_id	BC5
Number of Residues	11
Details	BINDING SITE FOR RESIDUE GOL A 714

Chain	Residue
A	LYS564
A	ILE566
A	HOH993
A	HOH994
A	HOH1012
A	GLU55
A	VAL366
A	PHE559
A	ASN560
A	THR561
A	ASN563

---

site_id	BC6
Number of Residues	6
Details	BINDING SITE FOR RESIDUE GOL A 715

Chain	Residue
A	ASN424
A	PHE426
A	ASP427
A	GLY428
A	SER429
A	HOH1035

---

site_id	BC7
Number of Residues	5
Details	BINDING SITE FOR RESIDUE GOL A 716

Chain	Residue
A	ASN207
A	ASN258
A	SER260
A	HIS591
A	GLN594

---

site_id	BC8
Number of Residues	9
Details	BINDING SITE FOR RESIDUE GOL A 717

Chain	Residue
A	PHE229
A	GLU328
A	ASP393
A	ASP394
A	PHE436
A	ARG446
A	TRS701
A	GOL704
A	GOL710

---

site_id	BC9
Number of Residues	3
Details	BINDING SITE FOR RESIDUE GOL A 718

Chain	Residue
A	TRP194
A	GLU271
A	1PE720

---

site_id	CC1
Number of Residues	3
Details	BINDING SITE FOR RESIDUE GOL A 719

Chain	Residue
A	ASP202
A	PRO203
A	ASP526

---

site_id	CC2
Number of Residues	9
Details	BINDING SITE FOR RESIDUE 1PE A 720

Chain	Residue
A	LEU204
A	PHE205
A	PHE208
A	TRP263
A	ARG266
A	ALA267
A	GOL718
A	HOH879
A	HOH1013

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1
Details	ACT_SITE: Nucleophile => ECO:0000269|PubMed:15023061, ECO:0000305|PubMed:10828446

Chain	Residue	Details
A	ASP286

---

site_id	SWS_FT_FI2
Number of Residues	1
Details	ACT_SITE: Proton donor => ECO:0000305|PubMed:10828446

Chain	Residue	Details
A	GLU328

---

site_id	SWS_FT_FI3
Number of Residues	7
Details	BINDING: BINDING => ECO:0000305|PubMed:11467966, ECO:0000305|PubMed:12364331, ECO:0000305|PubMed:15023061

Chain	Residue	Details
A	ASP144
A	HIS187
A	GLN254
A	ARG284
A	HIS392
A	ASP393
A	ARG509

---

site_id	SWS_FT_FI4
Number of Residues	1
Details	SITE: Transition state stabilizer => ECO:0000250

Chain	Residue	Details
A	ASP444

---