4F5L

A Theoretical Optimized Mutant for the Conversion of Substrate Specificity and Activity of Aspartate Aminotransferase to Tyrosine Aminotransferase: Chimera P7.

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003824	molecular_function	catalytic activity
A	0004069	molecular_function	L-aspartate:2-oxoglutarate aminotransferase activity
A	0004838	molecular_function	L-tyrosine-2-oxoglutarate transaminase activity
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0006520	biological_process	amino acid metabolic process
A	0008483	molecular_function	transaminase activity
A	0009058	biological_process	biosynthetic process
A	0009094	biological_process	L-phenylalanine biosynthetic process
A	0016740	molecular_function	transferase activity
A	0030170	molecular_function	pyridoxal phosphate binding
A	0033585	biological_process	L-phenylalanine biosynthetic process from chorismate via phenylpyruvate
A	0042802	molecular_function	identical protein binding
A	0042803	molecular_function	protein homodimerization activity
B	0003824	molecular_function	catalytic activity
B	0004069	molecular_function	L-aspartate:2-oxoglutarate aminotransferase activity
B	0004838	molecular_function	L-tyrosine-2-oxoglutarate transaminase activity
B	0005737	cellular_component	cytoplasm
B	0005829	cellular_component	cytosol
B	0006520	biological_process	amino acid metabolic process
B	0008483	molecular_function	transaminase activity
B	0009058	biological_process	biosynthetic process
B	0009094	biological_process	L-phenylalanine biosynthetic process
B	0016740	molecular_function	transferase activity
B	0030170	molecular_function	pyridoxal phosphate binding
B	0033585	biological_process	L-phenylalanine biosynthetic process from chorismate via phenylpyruvate
B	0042802	molecular_function	identical protein binding
B	0042803	molecular_function	protein homodimerization activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	4
Details	BINDING SITE FOR RESIDUE EDO A 501

Chain	Residue
A	GLN89
A	ARG106
A	THR107
A	HOH895

---

site_id	AC2
Number of Residues	5
Details	BINDING SITE FOR RESIDUE EDO A 502

Chain	Residue
A	GLN225
A	SER253
A	CYS254
A	ASN257
A	HOH612

---

site_id	AC3
Number of Residues	7
Details	BINDING SITE FOR RESIDUE EDO A 503

Chain	Residue
A	GLY230
A	LEU231
A	GLU318
A	LEU321
A	THR322
A	ARG325
B	GLU276

---

site_id	AC4
Number of Residues	11
Details	BINDING SITE FOR RESIDUE EDO A 504

Chain	Residue
A	ARG118
A	ASP122
A	SER149
A	ALA150
A	HOH773
A	HOH785
A	HOH1086
B	ARG118
B	ASP122
B	HOH1074
B	HOH1170

---

site_id	AC5
Number of Residues	6
Details	BINDING SITE FOR RESIDUE EDO A 505

Chain	Residue
A	ASP170
A	HOH621
B	LEU167
B	PHE169
B	GLN203
B	HOH1116

---

site_id	AC6
Number of Residues	6
Details	BINDING SITE FOR RESIDUE EDO A 506

Chain	Residue
A	VAL9
A	THR11
A	HOH730
B	GLY215
B	TRP216
B	GLU247

---

site_id	AC7
Number of Residues	7
Details	BINDING SITE FOR RESIDUE EDO A 507

Chain	Residue
A	GLY44
A	TRP140
A	ASN193
A	LLP256
A	PHE358
A	HOH664
A	HOH847

---

site_id	AC8
Number of Residues	10
Details	BINDING SITE FOR RESIDUE EDO B 501

Chain	Residue
B	PRO82
B	GLY85
B	ARG86
B	GLN89
B	ARG106
B	THR107
B	EDO505
B	HOH615
B	HOH1083
B	HOH1123

---

site_id	AC9
Number of Residues	5
Details	BINDING SITE FOR RESIDUE EDO B 502

Chain	Residue
B	GLN225
B	SER253
B	CYS254
B	ASN257
B	HOH627

---

site_id	BC1
Number of Residues	7
Details	BINDING SITE FOR RESIDUE EDO B 503

Chain	Residue
A	LEU167
A	PHE169
A	GLN203
A	HOH650
A	HOH696
B	ASP170
B	HOH622

---

site_id	BC2
Number of Residues	8
Details	BINDING SITE FOR RESIDUE EDO B 504

Chain	Residue
A	HOH781
B	TYR75
B	LEU76
B	GLY77
B	LEU78
B	SER295
B	HOH937
B	HOH1157

---

site_id	BC3
Number of Residues	8
Details	BINDING SITE FOR RESIDUE EDO B 505

Chain	Residue
B	GLN89
B	ILE100
B	LYS103
B	ARG104
B	ALA105
B	ARG106
B	EDO501
B	HOH891

---

site_id	BC4
Number of Residues	4
Details	BINDING SITE FOR RESIDUE EDO B 506

Chain	Residue
A	ASN137
A	GLU156
B	GLU163
B	ASN164

---

Functional Information from PROSITE/UniProt

site_id	PS00105
Number of Residues	14
Details	AA_TRANSFER_CLASS_1 Aminotransferases class-I pyridoxal-phosphate attachment site. SCSKnfGLyNERVG

Chain	Residue	Details
A	SER253-GLY266

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	2
Details	Binding site: {"evidences":[{"source":"PDB","id":"1ART","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1CZE","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3QPG","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4DBC","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	2
Details	Binding site: {"evidences":[{"source":"PDB","id":"1AIB","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1ART","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1CZE","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1SPA","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3QPG","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4DBC","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	2
Details	Binding site: {"evidences":[{"source":"PDB","id":"1AIB","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1ART","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1ASC","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1CZE","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1SPA","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3QPG","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4DBC","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	2
Details	Binding site: {"evidences":[{"source":"PDB","id":"1AHG","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1AIB","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1ARG","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1ART","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1CZE","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3QPG","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4DBC","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	2
Details	Modified residue: {"description":"N6-(pyridoxal phosphate)lysine","evidences":[{"source":"PubMed","id":"11148029","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"1993208","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"3298240","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"9891001","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1AAW","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1AHE","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1AHF","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1AHX","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1AHY","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1ARI","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1ARS","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1ASA","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1ASB","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1ASD","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1ASE","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1ASF","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1ASG","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1ASM","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1ASN","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1B4X","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1CZC","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1CZE","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1G4V","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1G4X","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1G7W","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1G7X","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1IX6","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1IX7","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1IX8","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1QIR","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1QIS","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1QIT","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1YOO","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2D5Y","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2D61","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2D63","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2D7Y","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3AAT","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3ZZJ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5EAA","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

Catalytic Information from CSA

site_id	MCSA1
Number of Residues	3
Details	M-CSA 777

Chain	Residue	Details
A	TRP140	steric role
A	ASP221	proton shuttle (general acid/base)
A	LLP256	proton shuttle (general acid/base)

---

site_id	MCSA2
Number of Residues	3
Details	M-CSA 777

Chain	Residue	Details
B	TRP140	steric role
B	ASP221	proton shuttle (general acid/base)
B	LLP256	proton shuttle (general acid/base)

---