4F40
X-ray crystal structure of Apo prostaglandin f synthase from Leishmania major Friedlin
Functional Information from GO Data
Chain | GOid | namespace | contents |
A | 0000166 | molecular_function | nucleotide binding |
A | 0001516 | biological_process | prostaglandin biosynthetic process |
A | 0004033 | molecular_function | aldo-keto reductase (NADPH) activity |
A | 0005737 | cellular_component | cytoplasm |
A | 0016491 | molecular_function | oxidoreductase activity |
A | 0016616 | molecular_function | oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor |
A | 0036130 | molecular_function | prostaglandin H2 endoperoxidase reductase activity |
B | 0000166 | molecular_function | nucleotide binding |
B | 0001516 | biological_process | prostaglandin biosynthetic process |
B | 0004033 | molecular_function | aldo-keto reductase (NADPH) activity |
B | 0005737 | cellular_component | cytoplasm |
B | 0016491 | molecular_function | oxidoreductase activity |
B | 0016616 | molecular_function | oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor |
B | 0036130 | molecular_function | prostaglandin H2 endoperoxidase reductase activity |
Functional Information from PDB Data
site_id | AC1 |
Number of Residues | 13 |
Details | BINDING SITE FOR RESIDUE CIT A 301 |
Chain | Residue |
A | GLY14 |
A | HOH476 |
A | HOH492 |
A | HOH596 |
A | HOH620 |
A | LYS16 |
A | TRP25 |
A | GLN26 |
A | EDO303 |
A | EDO305 |
A | EDO308 |
A | HOH414 |
A | HOH449 |
site_id | AC2 |
Number of Residues | 6 |
Details | BINDING SITE FOR RESIDUE EDO A 302 |
Chain | Residue |
A | PRO18 |
A | GLU72 |
A | ASP73 |
A | HOH450 |
A | HOH526 |
A | HOH698 |
site_id | AC3 |
Number of Residues | 7 |
Details | BINDING SITE FOR RESIDUE EDO A 303 |
Chain | Residue |
A | GLY14 |
A | LYS16 |
A | TRP25 |
A | PRO277 |
A | CIT301 |
A | HOH492 |
A | HOH630 |
site_id | AC4 |
Number of Residues | 6 |
Details | BINDING SITE FOR RESIDUE EDO A 304 |
Chain | Residue |
A | ASP118 |
A | CYS163 |
A | THR164 |
A | THR166 |
A | GLN283 |
A | HOH677 |
site_id | AC5 |
Number of Residues | 8 |
Details | BINDING SITE FOR RESIDUE EDO A 305 |
Chain | Residue |
A | ASN13 |
A | GLY14 |
A | VAL15 |
A | TRP81 |
A | PHE284 |
A | CIT301 |
A | HOH492 |
A | HOH568 |
site_id | AC6 |
Number of Residues | 5 |
Details | BINDING SITE FOR RESIDUE EDO A 306 |
Chain | Residue |
A | HIS154 |
A | GLU157 |
A | ASP158 |
A | HOH446 |
A | HOH474 |
site_id | AC7 |
Number of Residues | 5 |
Details | BINDING SITE FOR RESIDUE EDO A 307 |
Chain | Residue |
A | ALA52 |
A | TRP81 |
A | GLU84 |
A | HOH438 |
A | HOH506 |
site_id | AC8 |
Number of Residues | 7 |
Details | BINDING SITE FOR RESIDUE EDO A 308 |
Chain | Residue |
A | TRP25 |
A | TYR54 |
A | HIS112 |
A | ASN150 |
A | TRP197 |
A | CIT301 |
A | HOH473 |
site_id | AC9 |
Number of Residues | 7 |
Details | BINDING SITE FOR RESIDUE EDO A 309 |
Chain | Residue |
A | TYR138 |
A | LYS141 |
A | HOH523 |
A | HOH534 |
A | HOH559 |
A | HOH588 |
A | HOH629 |
site_id | BC1 |
Number of Residues | 6 |
Details | BINDING SITE FOR RESIDUE EDO A 310 |
Chain | Residue |
A | SER198 |
A | PRO199 |
A | LEU200 |
A | GLY201 |
A | PRO239 |
A | LYS240 |
site_id | BC2 |
Number of Residues | 3 |
Details | BINDING SITE FOR RESIDUE CL A 311 |
Chain | Residue |
A | ALA260 |
A | GLU261 |
A | HOH462 |
site_id | BC3 |
Number of Residues | 12 |
Details | BINDING SITE FOR RESIDUE CIT B 301 |
Chain | Residue |
B | GLY14 |
B | LYS16 |
B | TRP25 |
B | GLN26 |
B | EDO302 |
B | EDO307 |
B | HOH422 |
B | HOH442 |
B | HOH478 |
B | HOH499 |
B | HOH669 |
B | HOH698 |
site_id | BC4 |
Number of Residues | 7 |
Details | BINDING SITE FOR RESIDUE EDO B 302 |
Chain | Residue |
B | GLY14 |
B | LYS16 |
B | TRP25 |
B | PRO277 |
B | CIT301 |
B | HOH601 |
B | HOH698 |
site_id | BC5 |
Number of Residues | 5 |
Details | BINDING SITE FOR RESIDUE EDO B 303 |
Chain | Residue |
B | GLU72 |
B | HOH446 |
B | HOH525 |
B | HOH568 |
B | HOH694 |
site_id | BC6 |
Number of Residues | 8 |
Details | BINDING SITE FOR RESIDUE EDO B 304 |
Chain | Residue |
B | TRP81 |
B | PHE284 |
B | HOH517 |
B | HOH636 |
B | HOH698 |
B | HOH720 |
B | ASN13 |
B | GLY14 |
site_id | BC7 |
Number of Residues | 6 |
Details | BINDING SITE FOR RESIDUE EDO B 305 |
Chain | Residue |
B | TYR138 |
B | HOH475 |
B | HOH479 |
B | HOH490 |
B | HOH560 |
B | HOH585 |
site_id | BC8 |
Number of Residues | 4 |
Details | BINDING SITE FOR RESIDUE EDO B 306 |
Chain | Residue |
B | LYS240 |
B | SER241 |
B | VAL242 |
B | HIS243 |
site_id | BC9 |
Number of Residues | 7 |
Details | BINDING SITE FOR RESIDUE EDO B 307 |
Chain | Residue |
B | TRP25 |
B | TYR54 |
B | HIS112 |
B | ASN150 |
B | TRP197 |
B | CIT301 |
B | HOH465 |
site_id | CC1 |
Number of Residues | 4 |
Details | BINDING SITE FOR RESIDUE EDO B 308 |
Chain | Residue |
B | THR271 |
B | SER273 |
B | HOH489 |
B | HOH686 |
site_id | CC2 |
Number of Residues | 2 |
Details | BINDING SITE FOR RESIDUE EDO B 309 |
Chain | Residue |
B | ARG228 |
B | PHE254 |
site_id | CC3 |
Number of Residues | 4 |
Details | BINDING SITE FOR RESIDUE EDO B 310 |
Chain | Residue |
B | ALA52 |
B | TRP81 |
B | GLU84 |
B | HOH421 |
Functional Information from PROSITE/UniProt
site_id | PS00062 |
Number of Residues | 18 |
Details | ALDOKETO_REDUCTASE_2 Aldo/keto reductase family signature 2. FeqlykekkVRAIGVSNF |
Chain | Residue | Details |
A | PHE134-PHE151 |
site_id | PS00063 |
Number of Residues | 16 |
Details | ALDOKETO_REDUCTASE_3 Aldo/keto reductase family putative active site signature. IPKSVHreRIeENaDI |
Chain | Residue | Details |
A | ILE238-ILE253 |
site_id | PS00798 |
Number of Residues | 18 |
Details | ALDOKETO_REDUCTASE_1 Aldo/keto reductase family signature 1. GYRHIDTAaiyknEesVG |
Chain | Residue | Details |
A | GLY44-GLY61 |
Functional Information from SwissProt/UniProt
site_id | SWS_FT_FI1 |
Number of Residues | 2 |
Details | ACT_SITE: Proton donor => ECO:0000250 |
Chain | Residue | Details |
A | TYR54 | |
B | TYR54 |
site_id | SWS_FT_FI2 |
Number of Residues | 16 |
Details | BINDING: BINDING => ECO:0000250 |
Chain | Residue | Details |
A | VAL24 | |
B | ASP49 | |
B | HIS112 | |
B | SER149 | |
B | GLN171 | |
B | TRP197 | |
B | LYS240 | |
B | ARG246 | |
A | ASP49 | |
A | HIS112 | |
A | SER149 | |
A | GLN171 | |
A | TRP197 | |
A | LYS240 | |
A | ARG246 | |
B | VAL24 |
site_id | SWS_FT_FI3 |
Number of Residues | 2 |
Details | SITE: Lowers pKa of active site Tyr => ECO:0000250 |
Chain | Residue | Details |
A | LYS79 | |
B | LYS79 |