4F40
X-ray crystal structure of Apo prostaglandin f synthase from Leishmania major Friedlin
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0000166 | molecular_function | nucleotide binding |
| A | 0001516 | biological_process | prostaglandin biosynthetic process |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0006629 | biological_process | lipid metabolic process |
| A | 0006631 | biological_process | fatty acid metabolic process |
| A | 0006633 | biological_process | fatty acid biosynthetic process |
| A | 0016491 | molecular_function | oxidoreductase activity |
| A | 0016616 | molecular_function | oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor |
| B | 0000166 | molecular_function | nucleotide binding |
| B | 0001516 | biological_process | prostaglandin biosynthetic process |
| B | 0005737 | cellular_component | cytoplasm |
| B | 0006629 | biological_process | lipid metabolic process |
| B | 0006631 | biological_process | fatty acid metabolic process |
| B | 0006633 | biological_process | fatty acid biosynthetic process |
| B | 0016491 | molecular_function | oxidoreductase activity |
| B | 0016616 | molecular_function | oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 13 |
| Details | BINDING SITE FOR RESIDUE CIT A 301 |
| Chain | Residue |
| A | GLY14 |
| A | HOH476 |
| A | HOH492 |
| A | HOH596 |
| A | HOH620 |
| A | LYS16 |
| A | TRP25 |
| A | GLN26 |
| A | EDO303 |
| A | EDO305 |
| A | EDO308 |
| A | HOH414 |
| A | HOH449 |
| site_id | AC2 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE EDO A 302 |
| Chain | Residue |
| A | PRO18 |
| A | GLU72 |
| A | ASP73 |
| A | HOH450 |
| A | HOH526 |
| A | HOH698 |
| site_id | AC3 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE EDO A 303 |
| Chain | Residue |
| A | GLY14 |
| A | LYS16 |
| A | TRP25 |
| A | PRO277 |
| A | CIT301 |
| A | HOH492 |
| A | HOH630 |
| site_id | AC4 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE EDO A 304 |
| Chain | Residue |
| A | ASP118 |
| A | CYS163 |
| A | THR164 |
| A | THR166 |
| A | GLN283 |
| A | HOH677 |
| site_id | AC5 |
| Number of Residues | 8 |
| Details | BINDING SITE FOR RESIDUE EDO A 305 |
| Chain | Residue |
| A | ASN13 |
| A | GLY14 |
| A | VAL15 |
| A | TRP81 |
| A | PHE284 |
| A | CIT301 |
| A | HOH492 |
| A | HOH568 |
| site_id | AC6 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE EDO A 306 |
| Chain | Residue |
| A | HIS154 |
| A | GLU157 |
| A | ASP158 |
| A | HOH446 |
| A | HOH474 |
| site_id | AC7 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE EDO A 307 |
| Chain | Residue |
| A | ALA52 |
| A | TRP81 |
| A | GLU84 |
| A | HOH438 |
| A | HOH506 |
| site_id | AC8 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE EDO A 308 |
| Chain | Residue |
| A | TRP25 |
| A | TYR54 |
| A | HIS112 |
| A | ASN150 |
| A | TRP197 |
| A | CIT301 |
| A | HOH473 |
| site_id | AC9 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE EDO A 309 |
| Chain | Residue |
| A | TYR138 |
| A | LYS141 |
| A | HOH523 |
| A | HOH534 |
| A | HOH559 |
| A | HOH588 |
| A | HOH629 |
| site_id | BC1 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE EDO A 310 |
| Chain | Residue |
| A | SER198 |
| A | PRO199 |
| A | LEU200 |
| A | GLY201 |
| A | PRO239 |
| A | LYS240 |
| site_id | BC2 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE CL A 311 |
| Chain | Residue |
| A | ALA260 |
| A | GLU261 |
| A | HOH462 |
| site_id | BC3 |
| Number of Residues | 12 |
| Details | BINDING SITE FOR RESIDUE CIT B 301 |
| Chain | Residue |
| B | GLY14 |
| B | LYS16 |
| B | TRP25 |
| B | GLN26 |
| B | EDO302 |
| B | EDO307 |
| B | HOH422 |
| B | HOH442 |
| B | HOH478 |
| B | HOH499 |
| B | HOH669 |
| B | HOH698 |
| site_id | BC4 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE EDO B 302 |
| Chain | Residue |
| B | GLY14 |
| B | LYS16 |
| B | TRP25 |
| B | PRO277 |
| B | CIT301 |
| B | HOH601 |
| B | HOH698 |
| site_id | BC5 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE EDO B 303 |
| Chain | Residue |
| B | GLU72 |
| B | HOH446 |
| B | HOH525 |
| B | HOH568 |
| B | HOH694 |
| site_id | BC6 |
| Number of Residues | 8 |
| Details | BINDING SITE FOR RESIDUE EDO B 304 |
| Chain | Residue |
| B | TRP81 |
| B | PHE284 |
| B | HOH517 |
| B | HOH636 |
| B | HOH698 |
| B | HOH720 |
| B | ASN13 |
| B | GLY14 |
| site_id | BC7 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE EDO B 305 |
| Chain | Residue |
| B | TYR138 |
| B | HOH475 |
| B | HOH479 |
| B | HOH490 |
| B | HOH560 |
| B | HOH585 |
| site_id | BC8 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE EDO B 306 |
| Chain | Residue |
| B | LYS240 |
| B | SER241 |
| B | VAL242 |
| B | HIS243 |
| site_id | BC9 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE EDO B 307 |
| Chain | Residue |
| B | TRP25 |
| B | TYR54 |
| B | HIS112 |
| B | ASN150 |
| B | TRP197 |
| B | CIT301 |
| B | HOH465 |
| site_id | CC1 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE EDO B 308 |
| Chain | Residue |
| B | THR271 |
| B | SER273 |
| B | HOH489 |
| B | HOH686 |
| site_id | CC2 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE EDO B 309 |
| Chain | Residue |
| B | ARG228 |
| B | PHE254 |
| site_id | CC3 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE EDO B 310 |
| Chain | Residue |
| B | ALA52 |
| B | TRP81 |
| B | GLU84 |
| B | HOH421 |
Functional Information from PROSITE/UniProt
| site_id | PS00062 |
| Number of Residues | 18 |
| Details | ALDOKETO_REDUCTASE_2 Aldo/keto reductase family signature 2. FeqlykekkVRAIGVSNF |
| Chain | Residue | Details |
| A | PHE134-PHE151 |
| site_id | PS00063 |
| Number of Residues | 16 |
| Details | ALDOKETO_REDUCTASE_3 Aldo/keto reductase family putative active site signature. IPKSVHreRIeENaDI |
| Chain | Residue | Details |
| A | ILE238-ILE253 |
| site_id | PS00798 |
| Number of Residues | 18 |
| Details | ALDOKETO_REDUCTASE_1 Aldo/keto reductase family signature 1. GYRHIDTAaiyknEesVG |
| Chain | Residue | Details |
| A | GLY44-GLY61 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 2 |
| Details | Active site: {"description":"Proton donor","evidences":[{"evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 22 |
| Details | Binding site: {"evidences":[{"evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI3 |
| Number of Residues | 2 |
| Details | Site: {"description":"Lowers pKa of active site Tyr","evidences":[{"evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
