Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

4EWQ

Human p38 alpha MAPK in complex with a pyridazine based inhibitor

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000165	biological_process	MAPK cascade
A	0000166	molecular_function	nucleotide binding
A	0000922	cellular_component	spindle pole
A	0001502	biological_process	cartilage condensation
A	0001649	biological_process	osteoblast differentiation
A	0002021	biological_process	response to dietary excess
A	0002862	biological_process	negative regulation of inflammatory response to antigenic stimulus
A	0004672	molecular_function	protein kinase activity
A	0004674	molecular_function	protein serine/threonine kinase activity
A	0004707	molecular_function	MAP kinase activity
A	0004708	molecular_function	MAP kinase kinase activity
A	0005515	molecular_function	protein binding
A	0005524	molecular_function	ATP binding
A	0005576	cellular_component	extracellular region
A	0005634	cellular_component	nucleus
A	0005654	cellular_component	nucleoplasm
A	0005737	cellular_component	cytoplasm
A	0005739	cellular_component	mitochondrion
A	0005829	cellular_component	cytosol
A	0006351	biological_process	DNA-templated transcription
A	0006357	biological_process	regulation of transcription by RNA polymerase II
A	0006468	biological_process	protein phosphorylation
A	0006915	biological_process	apoptotic process
A	0006935	biological_process	chemotaxis
A	0007165	biological_process	signal transduction
A	0007166	biological_process	cell surface receptor signaling pathway
A	0010628	biological_process	positive regulation of gene expression
A	0010831	biological_process	positive regulation of myotube differentiation
A	0016301	molecular_function	kinase activity
A	0016607	cellular_component	nuclear speck
A	0016740	molecular_function	transferase activity
A	0019899	molecular_function	enzyme binding
A	0019903	molecular_function	protein phosphatase binding
A	0030168	biological_process	platelet activation
A	0030316	biological_process	osteoclast differentiation
A	0031098	biological_process	stress-activated protein kinase signaling cascade
A	0031281	biological_process	positive regulation of cyclase activity
A	0032735	biological_process	positive regulation of interleukin-12 production
A	0032868	biological_process	response to insulin
A	0033209	biological_process	tumor necrosis factor-mediated signaling pathway
A	0034774	cellular_component	secretory granule lumen
A	0035331	biological_process	negative regulation of hippo signaling
A	0035556	biological_process	intracellular signal transduction
A	0035924	biological_process	cellular response to vascular endothelial growth factor stimulus
A	0038066	biological_process	p38MAPK cascade
A	0042770	biological_process	signal transduction in response to DNA damage
A	0043536	biological_process	positive regulation of blood vessel endothelial cell migration
A	0045648	biological_process	positive regulation of erythrocyte differentiation
A	0045663	biological_process	positive regulation of myoblast differentiation
A	0048010	biological_process	vascular endothelial growth factor receptor signaling pathway
A	0048273	molecular_function	mitogen-activated protein kinase p38 binding
A	0051149	biological_process	positive regulation of muscle cell differentiation
A	0051403	biological_process	stress-activated MAPK cascade
A	0051525	molecular_function	NFAT protein binding
A	0060348	biological_process	bone development
A	0070935	biological_process	3'-UTR-mediated mRNA stabilization
A	0071222	biological_process	cellular response to lipopolysaccharide
A	0071223	biological_process	cellular response to lipoteichoic acid
A	0071479	biological_process	cellular response to ionizing radiation
A	0071493	biological_process	cellular response to UV-B
A	0090090	biological_process	negative regulation of canonical Wnt signaling pathway
A	0090398	biological_process	cellular senescence
A	0090400	biological_process	stress-induced premature senescence
A	0098586	biological_process	cellular response to virus
A	0098978	cellular_component	glutamatergic synapse
A	0099179	biological_process	regulation of synaptic membrane adhesion
A	0106310	molecular_function	protein serine kinase activity
A	1900015	biological_process	regulation of cytokine production involved in inflammatory response
A	1901741	biological_process	positive regulation of myoblast fusion
A	1904813	cellular_component	ficolin-1-rich granule lumen
A	2000379	biological_process	positive regulation of reactive oxygen species metabolic process

Functional Information from PDB Data

site_id	AC1
Number of Residues	11
Details	BINDING SITE FOR RESIDUE GG5 A 401

Chain	Residue
A	GLU192
A	SER293
A	HOH530
A	LEU195
A	TRP197
A	PRO242
A	LEU246
A	LYS249
A	ILE250
A	ILE259
A	LEU291

site_id	AC2
Number of Residues	13
Details	BINDING SITE FOR RESIDUE MWL A 402

Chain	Residue
A	TYR35
A	ALA51
A	LYS53
A	ILE84
A	LEU104
A	THR106
A	HIS107
A	LEU108
A	MET109
A	SER154
A	ASN155
A	LEU167
A	ASP168

site_id	AC3
Number of Residues	11
Details	BINDING SITE FOR RESIDUE MWL A 403

Chain	Residue
A	THR7
A	GLU22
A	ARG23
A	THR44
A	ILE229
A	SER254
A	ASN257
A	TYR258
A	ALA271
A	PRO279
A	VAL282

site_id	AC4
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN A 404

Chain	Residue
A	HIS64
A	ASP161
A	GLU178
A	TPO180

site_id	AC5
Number of Residues	4
Details	BINDING SITE FOR RESIDUE CL A 405

Chain	Residue
A	HIS142
A	GLN202
A	ALA299
A	HOH591

site_id	AC6
Number of Residues	2
Details	BINDING SITE FOR RESIDUE BME A 406

Chain	Residue
A	GLN120
A	CYS162

site_id	AC7
Number of Residues	8
Details	BINDING SITE FOR RESIDUE GOL A 407

Chain	Residue
A	PHE8
A	ARG10
A	GLU22
A	MET268
A	ASN269
A	PHE270
A	ALA271
A	GLU286

site_id	AC8
Number of Residues	3
Details	BINDING SITE FOR RESIDUE ACT A 408

Chain	Residue
A	PRO6
A	ASP88
A	VAL89

site_id	AC9
Number of Residues	5
Details	BINDING SITE FOR RESIDUE PEG A 409

Chain	Residue
A	GLN128
A	GLN264
A	LEU280
A	ALA306
A	ALA309

Functional Information from PROSITE/UniProt

site_id	PS00107
Number of Residues	25
Details	PROTEIN_KINASE_ATP Protein kinases ATP-binding region signature. VGSGAYGSVCaAfdtktglrv.........AVKK

Chain	Residue	Details
A	VAL30-LYS54

site_id	PS01351
Number of Residues	104
Details	MAPK MAP kinase signature. FqsiihakrtyRElrllkhmkhenviglldvftparsleefndvylvthlmgadlnnivkcqkltddhvqfliyqilrglkyihsadiih.........RDlKpsnlavnedC

Chain	Residue	Details
A	PHE59-CYS162

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1
Details	Active site: {"description":"Proton acceptor"}

Chain

Residue

Details

site_id	SWS_FT_FI2
Number of Residues	9
Details	Binding site: {}

Chain

Residue

Details

site_id	SWS_FT_FI3
Number of Residues	1
Details	Modified residue: {"description":"Phosphothreonine","evidences":[{"source":"PubMed","id":"18691976","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI4
Number of Residues	2
Details	Modified residue: {"description":"N6-acetyllysine","evidences":[{"source":"PubMed","id":"21444723","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI5
Number of Residues	1
Details	Modified residue: {"description":"Phosphothreonine; by MAP2K3, MAP2K4, MAP2K6 and autocatalysis","evidences":[{"source":"PubMed","id":"7535770","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"18669648","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"19690332","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"20068231","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"23186163","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI6
Number of Residues	1
Details	Modified residue: {"description":"Phosphotyrosine; by MAP2K3, MAP2K4, MAP2K6 and autocatalysis","evidences":[{"source":"PubMed","id":"7535770","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"18669648","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"19690332","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"20068231","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"23186163","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI7
Number of Residues	1
Details	Modified residue: {"description":"Phosphothreonine","evidences":[{"source":"PubMed","id":"17525332","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI8
Number of Residues	1
Details	Modified residue: {"description":"Phosphotyrosine; by ZAP70","evidences":[{"source":"PubMed","id":"15735648","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)