4EBP
BlaC E166A Cefotaxime Acyl-Intermediate Complex
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0005576 | cellular_component | extracellular region |
| A | 0005886 | cellular_component | plasma membrane |
| A | 0008800 | molecular_function | beta-lactamase activity |
| A | 0016787 | molecular_function | hydrolase activity |
| A | 0017001 | biological_process | antibiotic catabolic process |
| A | 0030655 | biological_process | beta-lactam antibiotic catabolic process |
| A | 0033250 | molecular_function | penicillinase activity |
| A | 0033251 | molecular_function | cephalosporinase activity |
| A | 0042597 | cellular_component | periplasmic space |
| A | 0046677 | biological_process | response to antibiotic |
| B | 0005576 | cellular_component | extracellular region |
| B | 0005886 | cellular_component | plasma membrane |
| B | 0008800 | molecular_function | beta-lactamase activity |
| B | 0016787 | molecular_function | hydrolase activity |
| B | 0017001 | biological_process | antibiotic catabolic process |
| B | 0030655 | biological_process | beta-lactam antibiotic catabolic process |
| B | 0033250 | molecular_function | penicillinase activity |
| B | 0033251 | molecular_function | cephalosporinase activity |
| B | 0042597 | cellular_component | periplasmic space |
| B | 0046677 | biological_process | response to antibiotic |
| C | 0005576 | cellular_component | extracellular region |
| C | 0005886 | cellular_component | plasma membrane |
| C | 0008800 | molecular_function | beta-lactamase activity |
| C | 0016787 | molecular_function | hydrolase activity |
| C | 0017001 | biological_process | antibiotic catabolic process |
| C | 0030655 | biological_process | beta-lactam antibiotic catabolic process |
| C | 0033250 | molecular_function | penicillinase activity |
| C | 0033251 | molecular_function | cephalosporinase activity |
| C | 0042597 | cellular_component | periplasmic space |
| C | 0046677 | biological_process | response to antibiotic |
| D | 0005576 | cellular_component | extracellular region |
| D | 0005886 | cellular_component | plasma membrane |
| D | 0008800 | molecular_function | beta-lactamase activity |
| D | 0016787 | molecular_function | hydrolase activity |
| D | 0017001 | biological_process | antibiotic catabolic process |
| D | 0030655 | biological_process | beta-lactam antibiotic catabolic process |
| D | 0033250 | molecular_function | penicillinase activity |
| D | 0033251 | molecular_function | cephalosporinase activity |
| D | 0042597 | cellular_component | periplasmic space |
| D | 0046677 | biological_process | response to antibiotic |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE PO4 A 301 |
| Chain | Residue |
| A | SER70 |
| A | SER130 |
| A | THR235 |
| A | THR237 |
| A | HOH404 |
| B | GLN110 |
| B | GLN111 |
| site_id | AC2 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE PO4 A 302 |
| Chain | Residue |
| A | ASP240 |
| A | TYR272 |
| B | SER99 |
| B | GLN114 |
| A | ASN170 |
| A | ARG171 |
| site_id | AC3 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE PO4 A 303 |
| Chain | Residue |
| A | ASP124 |
| A | ARG128 |
| A | ARG213 |
| site_id | AC4 |
| Number of Residues | 1 |
| Details | BINDING SITE FOR RESIDUE PO4 A 304 |
| Chain | Residue |
| A | GLU64 |
| site_id | AC5 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE PCZ B 301 |
| Chain | Residue |
| B | SER70 |
| B | SER130 |
| B | THR235 |
| B | GLY236 |
| B | THR237 |
| B | HOH420 |
| B | HOH434 |
| site_id | AC6 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE PO4 B 302 |
| Chain | Residue |
| B | ASP124 |
| B | ARG128 |
| B | ARG213 |
| site_id | AC7 |
| Number of Residues | 9 |
| Details | BINDING SITE FOR RESIDUE PCZ C 301 |
| Chain | Residue |
| C | SER70 |
| C | ILE105 |
| C | SER130 |
| C | THR235 |
| C | GLY236 |
| C | THR237 |
| C | ASP240 |
| C | HOH432 |
| D | GLN111 |
| site_id | AC8 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE PO4 C 302 |
| Chain | Residue |
| C | ASP124 |
| C | ARG128 |
| C | ARG213 |
| site_id | AC9 |
| Number of Residues | 8 |
| Details | BINDING SITE FOR RESIDUE PO4 D 301 |
| Chain | Residue |
| C | GLN110 |
| C | GLN111 |
| D | SER70 |
| D | SER130 |
| D | THR216 |
| D | THR235 |
| D | GLY236 |
| D | THR237 |
| site_id | BC1 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE PO4 D 302 |
| Chain | Residue |
| D | ASP124 |
| D | ARG128 |
| D | ARG213 |
| site_id | BC2 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE PO4 D 303 |
| Chain | Residue |
| D | ARG61 |
| D | GLU64 |
Functional Information from PROSITE/UniProt
| site_id | PS00146 |
| Number of Residues | 16 |
| Details | BETA_LACTAMASE_A Beta-lactamase class-A active site. FaFCSTfKaplVAAVL |
| Chain | Residue | Details |
| A | PHE66-LEU81 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 4 |
| Details | ACT_SITE: Acyl-ester intermediate => ECO:0000269|PubMed:19251630, ECO:0000269|PubMed:20353175, ECO:0000269|PubMed:20961112 |
| Chain | Residue | Details |
| A | SER70 | |
| B | SER70 | |
| C | SER70 | |
| D | SER70 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 4 |
| Details | ACT_SITE: Proton acceptor => ECO:0000303|PubMed:20353175, ECO:0000303|PubMed:20961112 |
| Chain | Residue | Details |
| A | ALA166 | |
| B | ALA166 | |
| C | ALA166 | |
| D | ALA166 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 8 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:19251630, ECO:0000269|PubMed:20353175, ECO:0000269|PubMed:20961112 |
| Chain | Residue | Details |
| C | SER130 | |
| C | THR235 | |
| D | SER130 | |
| D | THR235 | |
| A | SER130 | |
| A | THR235 | |
| B | SER130 | |
| B | THR235 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 4 |
| Details | SITE: Increases nucleophilicity of active site Ser => ECO:0000303|PubMed:20353175, ECO:0000303|PubMed:20961112 |
| Chain | Residue | Details |
| A | LYS73 | |
| B | LYS73 | |
| C | LYS73 | |
| D | LYS73 |
| site_id | SWS_FT_FI5 |
| Number of Residues | 4 |
| Details | SITE: Functions as a gatekeeper residue that regulates substrate accessibility to the enzyme active site => ECO:0000303|PubMed:24023821 |
| Chain | Residue | Details |
| A | ILE105 | |
| B | ILE105 | |
| C | ILE105 | |
| D | ILE105 |
