4EBP

BlaC E166A Cefotaxime Acyl-Intermediate Complex

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0005576	cellular_component	extracellular region
A	0005886	cellular_component	plasma membrane
A	0008800	molecular_function	beta-lactamase activity
A	0016787	molecular_function	hydrolase activity
A	0017001	biological_process	antibiotic catabolic process
A	0030655	biological_process	beta-lactam antibiotic catabolic process
A	0042597	cellular_component	periplasmic space
A	0046677	biological_process	response to antibiotic
B	0005576	cellular_component	extracellular region
B	0005886	cellular_component	plasma membrane
B	0008800	molecular_function	beta-lactamase activity
B	0016787	molecular_function	hydrolase activity
B	0017001	biological_process	antibiotic catabolic process
B	0030655	biological_process	beta-lactam antibiotic catabolic process
B	0042597	cellular_component	periplasmic space
B	0046677	biological_process	response to antibiotic
C	0005576	cellular_component	extracellular region
C	0005886	cellular_component	plasma membrane
C	0008800	molecular_function	beta-lactamase activity
C	0016787	molecular_function	hydrolase activity
C	0017001	biological_process	antibiotic catabolic process
C	0030655	biological_process	beta-lactam antibiotic catabolic process
C	0042597	cellular_component	periplasmic space
C	0046677	biological_process	response to antibiotic
D	0005576	cellular_component	extracellular region
D	0005886	cellular_component	plasma membrane
D	0008800	molecular_function	beta-lactamase activity
D	0016787	molecular_function	hydrolase activity
D	0017001	biological_process	antibiotic catabolic process
D	0030655	biological_process	beta-lactam antibiotic catabolic process
D	0042597	cellular_component	periplasmic space
D	0046677	biological_process	response to antibiotic

Functional Information from PDB Data

site_id	AC1
Number of Residues	7
Details	BINDING SITE FOR RESIDUE PO4 A 301

Chain	Residue
A	SER70
A	SER130
A	THR235
A	THR237
A	HOH404
B	GLN110
B	GLN111

---

site_id	AC2
Number of Residues	6
Details	BINDING SITE FOR RESIDUE PO4 A 302

Chain	Residue
A	ASP240
A	TYR272
B	SER99
B	GLN114
A	ASN170
A	ARG171

---

site_id	AC3
Number of Residues	3
Details	BINDING SITE FOR RESIDUE PO4 A 303

Chain	Residue
A	ASP124
A	ARG128
A	ARG213

---

site_id	AC4
Number of Residues	1
Details	BINDING SITE FOR RESIDUE PO4 A 304

Chain	Residue
A	GLU64

---

site_id	AC5
Number of Residues	7
Details	BINDING SITE FOR RESIDUE PCZ B 301

Chain	Residue
B	SER70
B	SER130
B	THR235
B	GLY236
B	THR237
B	HOH420
B	HOH434

---

site_id	AC6
Number of Residues	3
Details	BINDING SITE FOR RESIDUE PO4 B 302

Chain	Residue
B	ASP124
B	ARG128
B	ARG213

---

site_id	AC7
Number of Residues	9
Details	BINDING SITE FOR RESIDUE PCZ C 301

Chain	Residue
C	SER70
C	ILE105
C	SER130
C	THR235
C	GLY236
C	THR237
C	ASP240
C	HOH432
D	GLN111

---

site_id	AC8
Number of Residues	3
Details	BINDING SITE FOR RESIDUE PO4 C 302

Chain	Residue
C	ASP124
C	ARG128
C	ARG213

---

site_id	AC9
Number of Residues	8
Details	BINDING SITE FOR RESIDUE PO4 D 301

Chain	Residue
C	GLN110
C	GLN111
D	SER70
D	SER130
D	THR216
D	THR235
D	GLY236
D	THR237

---

site_id	BC1
Number of Residues	3
Details	BINDING SITE FOR RESIDUE PO4 D 302

Chain	Residue
D	ASP124
D	ARG128
D	ARG213

---

site_id	BC2
Number of Residues	2
Details	BINDING SITE FOR RESIDUE PO4 D 303

Chain	Residue
D	ARG61
D	GLU64

---

Functional Information from PROSITE/UniProt

site_id	PS00146
Number of Residues	16
Details	BETA_LACTAMASE_A Beta-lactamase class-A active site. FaFCSTfKaplVAAVL

Chain	Residue	Details
A	PHE66-LEU81

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	4
Details	ACT_SITE: Acyl-ester intermediate => ECO:0000269|PubMed:19251630, ECO:0000269|PubMed:20353175, ECO:0000269|PubMed:20961112

Chain	Residue	Details
A	SER70
B	SER70
C	SER70
D	SER70

---

site_id	SWS_FT_FI2
Number of Residues	4
Details	ACT_SITE: Proton acceptor => ECO:0000303|PubMed:20353175, ECO:0000303|PubMed:20961112

Chain	Residue	Details
A	ALA166
B	ALA166
C	ALA166
D	ALA166

---

site_id	SWS_FT_FI3
Number of Residues	8
Details	BINDING: BINDING => ECO:0000269|PubMed:19251630, ECO:0000269|PubMed:20353175, ECO:0000269|PubMed:20961112

Chain	Residue	Details
A	SER130
A	THR235
B	SER130
B	THR235
C	SER130
C	THR235
D	SER130
D	THR235

---

site_id	SWS_FT_FI4
Number of Residues	4
Details	SITE: Increases nucleophilicity of active site Ser => ECO:0000303|PubMed:20353175, ECO:0000303|PubMed:20961112

Chain	Residue	Details
A	LYS73
B	LYS73
C	LYS73
D	LYS73

---

site_id	SWS_FT_FI5
Number of Residues	4
Details	SITE: Functions as a gatekeeper residue that regulates substrate accessibility to the enzyme active site => ECO:0000303|PubMed:24023821

Chain	Residue	Details
A	ILE105
B	ILE105
C	ILE105
D	ILE105

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