Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

4EAH

Crystal structure of the formin homology 2 domain of FMNL3 bound to actin

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0007010	biological_process	cytoskeleton organization
A	0022604	biological_process	regulation of cell morphogenesis
B	0007010	biological_process	cytoskeleton organization
B	0022604	biological_process	regulation of cell morphogenesis
C	0007010	biological_process	cytoskeleton organization
C	0022604	biological_process	regulation of cell morphogenesis
D	0000287	molecular_function	magnesium ion binding
D	0001725	cellular_component	stress fiber
D	0003785	molecular_function	actin monomer binding
D	0005509	molecular_function	calcium ion binding
D	0005515	molecular_function	protein binding
D	0005523	molecular_function	tropomyosin binding
D	0005524	molecular_function	ATP binding
D	0005737	cellular_component	cytoplasm
D	0005856	cellular_component	cytoskeleton
D	0005865	cellular_component	striated muscle thin filament
D	0005884	cellular_component	actin filament
D	0010628	biological_process	positive regulation of gene expression
D	0016787	molecular_function	hydrolase activity
D	0019904	molecular_function	protein domain specific binding
D	0030027	cellular_component	lamellipodium
D	0030041	biological_process	actin filament polymerization
D	0030175	cellular_component	filopodium
D	0030240	biological_process	skeletal muscle thin filament assembly
D	0031013	molecular_function	troponin I binding
D	0031432	molecular_function	titin binding
D	0031941	cellular_component	filamentous actin
D	0032036	molecular_function	myosin heavy chain binding
D	0032432	cellular_component	actin filament bundle
D	0042802	molecular_function	identical protein binding
D	0044297	cellular_component	cell body
D	0048306	molecular_function	calcium-dependent protein binding
D	0048741	biological_process	skeletal muscle fiber development
D	0051017	biological_process	actin filament bundle assembly
D	0090131	biological_process	mesenchyme migration
D	0098723	cellular_component	skeletal muscle myofibril
D	0140660	molecular_function	cytoskeletal motor activator activity
E	0007010	biological_process	cytoskeleton organization
E	0022604	biological_process	regulation of cell morphogenesis
F	0000287	molecular_function	magnesium ion binding
F	0001725	cellular_component	stress fiber
F	0003785	molecular_function	actin monomer binding
F	0005509	molecular_function	calcium ion binding
F	0005515	molecular_function	protein binding
F	0005523	molecular_function	tropomyosin binding
F	0005524	molecular_function	ATP binding
F	0005737	cellular_component	cytoplasm
F	0005856	cellular_component	cytoskeleton
F	0005865	cellular_component	striated muscle thin filament
F	0005884	cellular_component	actin filament
F	0010628	biological_process	positive regulation of gene expression
F	0016787	molecular_function	hydrolase activity
F	0019904	molecular_function	protein domain specific binding
F	0030027	cellular_component	lamellipodium
F	0030041	biological_process	actin filament polymerization
F	0030175	cellular_component	filopodium
F	0030240	biological_process	skeletal muscle thin filament assembly
F	0031013	molecular_function	troponin I binding
F	0031432	molecular_function	titin binding
F	0031941	cellular_component	filamentous actin
F	0032036	molecular_function	myosin heavy chain binding
F	0032432	cellular_component	actin filament bundle
F	0042802	molecular_function	identical protein binding
F	0044297	cellular_component	cell body
F	0048306	molecular_function	calcium-dependent protein binding
F	0048741	biological_process	skeletal muscle fiber development
F	0051017	biological_process	actin filament bundle assembly
F	0090131	biological_process	mesenchyme migration
F	0098723	cellular_component	skeletal muscle myofibril
F	0140660	molecular_function	cytoskeletal motor activator activity
G	0000287	molecular_function	magnesium ion binding
G	0001725	cellular_component	stress fiber
G	0003785	molecular_function	actin monomer binding
G	0005509	molecular_function	calcium ion binding
G	0005515	molecular_function	protein binding
G	0005523	molecular_function	tropomyosin binding
G	0005524	molecular_function	ATP binding
G	0005737	cellular_component	cytoplasm
G	0005856	cellular_component	cytoskeleton
G	0005865	cellular_component	striated muscle thin filament
G	0005884	cellular_component	actin filament
G	0010628	biological_process	positive regulation of gene expression
G	0016787	molecular_function	hydrolase activity
G	0019904	molecular_function	protein domain specific binding
G	0030027	cellular_component	lamellipodium
G	0030041	biological_process	actin filament polymerization
G	0030175	cellular_component	filopodium
G	0030240	biological_process	skeletal muscle thin filament assembly
G	0031013	molecular_function	troponin I binding
G	0031432	molecular_function	titin binding
G	0031941	cellular_component	filamentous actin
G	0032036	molecular_function	myosin heavy chain binding
G	0032432	cellular_component	actin filament bundle
G	0042802	molecular_function	identical protein binding
G	0044297	cellular_component	cell body
G	0048306	molecular_function	calcium-dependent protein binding
G	0048741	biological_process	skeletal muscle fiber development
G	0051017	biological_process	actin filament bundle assembly
G	0090131	biological_process	mesenchyme migration
G	0098723	cellular_component	skeletal muscle myofibril
G	0140660	molecular_function	cytoskeletal motor activator activity
H	0000287	molecular_function	magnesium ion binding
H	0001725	cellular_component	stress fiber
H	0003785	molecular_function	actin monomer binding
H	0005509	molecular_function	calcium ion binding
H	0005515	molecular_function	protein binding
H	0005523	molecular_function	tropomyosin binding
H	0005524	molecular_function	ATP binding
H	0005737	cellular_component	cytoplasm
H	0005856	cellular_component	cytoskeleton
H	0005865	cellular_component	striated muscle thin filament
H	0005884	cellular_component	actin filament
H	0010628	biological_process	positive regulation of gene expression
H	0016787	molecular_function	hydrolase activity
H	0019904	molecular_function	protein domain specific binding
H	0030027	cellular_component	lamellipodium
H	0030041	biological_process	actin filament polymerization
H	0030175	cellular_component	filopodium
H	0030240	biological_process	skeletal muscle thin filament assembly
H	0031013	molecular_function	troponin I binding
H	0031432	molecular_function	titin binding
H	0031941	cellular_component	filamentous actin
H	0032036	molecular_function	myosin heavy chain binding
H	0032432	cellular_component	actin filament bundle
H	0042802	molecular_function	identical protein binding
H	0044297	cellular_component	cell body
H	0048306	molecular_function	calcium-dependent protein binding
H	0048741	biological_process	skeletal muscle fiber development
H	0051017	biological_process	actin filament bundle assembly
H	0090131	biological_process	mesenchyme migration
H	0098723	cellular_component	skeletal muscle myofibril
H	0140660	molecular_function	cytoskeletal motor activator activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	7
Details	BINDING SITE FOR RESIDUE ACT D 401

Chain	Residue
D	PHE21
D	ALA22
D	GLY23
D	ASP24
D	ASP25
D	ALA26
D	ARG28

site_id	AC2
Number of Residues	17
Details	BINDING SITE FOR RESIDUE ATP D 402

Chain	Residue
D	SER14
D	GLY15
D	LEU16
D	LYS18
D	GLN137
D	ASP154
D	GLY156
D	ASP157
D	GLY158
D	LYS213
D	GLU214
D	GLY302
D	MET305
D	TYR306
D	LYS336
D	ASP11
D	GLY13

site_id	AC3
Number of Residues	5
Details	BINDING SITE FOR RESIDUE ACT A 1001

Chain	Residue
A	PHE574
A	ASN575
A	TRP576
E	ASN778
E	ARG782

site_id	AC4
Number of Residues	5
Details	BINDING SITE FOR RESIDUE ACT A 1002

Chain	Residue
A	ARG804
E	THR612
G	GLN121
G	GLU125
G	THR126

site_id	AC5
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ACT A 1003

Chain	Residue
A	ASN778
E	VAL573
E	PHE574
E	TRP576

site_id	AC6
Number of Residues	3
Details	BINDING SITE FOR RESIDUE ACT E 1001

Chain	Residue
A	THR612
D	GLU125
E	ARG804

site_id	AC7
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ACT C 1001

Chain	Residue
B	ASN778
C	PHE574
C	ASN575
C	TRP576

site_id	AC8
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ACT C 1002

Chain	Residue
C	ARG804
H	GLN121
H	GLU125
H	THR126

site_id	AC9
Number of Residues	5
Details	BINDING SITE FOR RESIDUE ACT B 1001

Chain	Residue
B	PHE574
B	ASN575
B	TRP576
C	ASN778
C	ARG782

site_id	BC1
Number of Residues	7
Details	BINDING SITE FOR RESIDUE ACT H 401

Chain	Residue
H	PHE21
H	ALA22
H	GLY23
H	ASP24
H	ASP25
H	ALA26
H	ARG28

site_id	BC2
Number of Residues	18
Details	BINDING SITE FOR RESIDUE ATP H 402

Chain	Residue
H	ASP11
H	GLY13
H	SER14
H	GLY15
H	LEU16
H	LYS18
H	GLN137
H	ASP154
H	GLY156
H	ASP157
H	GLY158
H	LYS213
H	GLU214
H	GLY301
H	GLY302
H	MET305
H	TYR306
H	LYS336

site_id	BC3
Number of Residues	7
Details	BINDING SITE FOR RESIDUE ACT G 401

Chain	Residue
G	PHE21
G	ALA22
G	GLY23
G	ASP24
G	ASP25
G	ALA26
G	ARG28

site_id	BC4
Number of Residues	19
Details	BINDING SITE FOR RESIDUE ATP G 402

Chain	Residue
G	GLY301
G	GLY302
G	THR303
G	MET305
G	TYR306
G	LYS336
G	ASP11
G	GLY13
G	SER14
G	GLY15
G	LEU16
G	LYS18
G	GLN137
G	ASP154
G	GLY156
G	ASP157
G	ARG210
G	LYS213
G	GLU214

site_id	BC5
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ACT F 401

Chain	Residue
B	ARG804
C	THR612
F	GLN121
F	GLU125

site_id	BC6
Number of Residues	6
Details	BINDING SITE FOR RESIDUE ACT F 402

Chain	Residue
F	PHE21
F	ALA22
F	GLY23
F	ASP24
F	ASP25
F	ARG28

site_id	BC7
Number of Residues	15
Details	BINDING SITE FOR RESIDUE ATP F 403

Chain	Residue
F	ASP11
F	GLY13
F	SER14
F	GLY15
F	LEU16
F	LYS18
F	GLN137
F	ASP154
F	GLY156
F	ASP157
F	LYS213
F	GLU214
F	GLY302
F	MET305
F	LYS336

Functional Information from PROSITE/UniProt

site_id	PS00406
Number of Residues	11
Details	ACTINS_1 Actins signature 1. YVGDEAQs.KRG

Chain	Residue	Details
D	TYR53-GLY63

site_id	PS00432
Number of Residues	9
Details	ACTINS_2 Actins signature 2. WITKqEYDE

Chain	Residue	Details
D	TRP356-GLU364

site_id	PS01132
Number of Residues	13
Details	ACTINS_ACT_LIKE Actins and actin-related proteins signature. LLTEApLNPkaNR

Chain	Residue	Details
D	LEU104-ARG116

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	4
Details	MOD_RES: N-acetylcysteine; in intermediate form => ECO:0000250\|UniProtKB:P62737

Chain	Residue	Details
D	CYS0
H	CYS0
G	CYS0
F	CYS0

site_id	SWS_FT_FI2
Number of Residues	4
Details	MOD_RES: N-acetylaspartate; in Actin, alpha skeletal muscle => ECO:0000269\|PubMed:1150665

Chain	Residue	Details
D	ASP1
H	ASP1
G	ASP1
F	ASP1

site_id	SWS_FT_FI3
Number of Residues	8
Details	MOD_RES: Methionine (R)-sulfoxide => ECO:0000250\|UniProtKB:P68134

Chain	Residue	Details
D	MET44
D	MET47
H	MET44
H	MET47
G	MET44
G	MET47
F	MET44
F	MET47

site_id	SWS_FT_FI4
Number of Residues	4
Details	MOD_RES: N6-malonyllysine => ECO:0000250

Chain	Residue	Details
D	LYS61
H	LYS61
G	LYS61
F	LYS61

site_id	SWS_FT_FI5
Number of Residues	4
Details	MOD_RES: Tele-methylhistidine => ECO:0000269\|PubMed:1150665, ECO:0000269\|PubMed:16905096, ECO:0000269\|PubMed:213279, ECO:0000269\|PubMed:2395459, ECO:0000269\|PubMed:499690, ECO:0007744\|PDB:1ATN, ECO:0007744\|PDB:1NWK

Chain	Residue	Details
D	HIS73
H	HIS73
G	HIS73
F	HIS73

site_id	SWS_FT_FI6
Number of Residues	4
Details	MOD_RES: N6-methyllysine => ECO:0000250\|UniProtKB:P68133

Chain	Residue	Details
D	LYS84
H	LYS84
G	LYS84
F	LYS84

site_id	SWS_FT_FI7
Number of Residues	4
Details	MOD_RES: ADP-ribosylarginine; by SpvB => ECO:0000305\|PubMed:16905096

Chain	Residue	Details
D	ARG177
H	ARG177
G	ARG177
F	ARG177

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)