Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

4E5N

Thermostable phosphite dehydrogenase in complex with NAD

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0005829	cellular_component	cytosol
A	0008465	molecular_function	hydroxypyruvate reductase (NADH) activity
A	0016491	molecular_function	oxidoreductase activity
A	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
A	0016618	molecular_function	hydroxypyruvate reductase [NAD(P)H] activity
A	0030267	molecular_function	glyoxylate reductase (NADPH) activity
A	0050609	molecular_function	phosphonate dehydrogenase activity
A	0051287	molecular_function	NAD binding
B	0005829	cellular_component	cytosol
B	0008465	molecular_function	hydroxypyruvate reductase (NADH) activity
B	0016491	molecular_function	oxidoreductase activity
B	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
B	0016618	molecular_function	hydroxypyruvate reductase [NAD(P)H] activity
B	0030267	molecular_function	glyoxylate reductase (NADPH) activity
B	0050609	molecular_function	phosphonate dehydrogenase activity
B	0051287	molecular_function	NAD binding
C	0005829	cellular_component	cytosol
C	0008465	molecular_function	hydroxypyruvate reductase (NADH) activity
C	0016491	molecular_function	oxidoreductase activity
C	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
C	0016618	molecular_function	hydroxypyruvate reductase [NAD(P)H] activity
C	0030267	molecular_function	glyoxylate reductase (NADPH) activity
C	0050609	molecular_function	phosphonate dehydrogenase activity
C	0051287	molecular_function	NAD binding
D	0005829	cellular_component	cytosol
D	0008465	molecular_function	hydroxypyruvate reductase (NADH) activity
D	0016491	molecular_function	oxidoreductase activity
D	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
D	0016618	molecular_function	hydroxypyruvate reductase [NAD(P)H] activity
D	0030267	molecular_function	glyoxylate reductase (NADPH) activity
D	0050609	molecular_function	phosphonate dehydrogenase activity
D	0051287	molecular_function	NAD binding
E	0005829	cellular_component	cytosol
E	0008465	molecular_function	hydroxypyruvate reductase (NADH) activity
E	0016491	molecular_function	oxidoreductase activity
E	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
E	0016618	molecular_function	hydroxypyruvate reductase [NAD(P)H] activity
E	0030267	molecular_function	glyoxylate reductase (NADPH) activity
E	0050609	molecular_function	phosphonate dehydrogenase activity
E	0051287	molecular_function	NAD binding
F	0005829	cellular_component	cytosol
F	0008465	molecular_function	hydroxypyruvate reductase (NADH) activity
F	0016491	molecular_function	oxidoreductase activity
F	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
F	0016618	molecular_function	hydroxypyruvate reductase [NAD(P)H] activity
F	0030267	molecular_function	glyoxylate reductase (NADPH) activity
F	0050609	molecular_function	phosphonate dehydrogenase activity
F	0051287	molecular_function	NAD binding
G	0005829	cellular_component	cytosol
G	0008465	molecular_function	hydroxypyruvate reductase (NADH) activity
G	0016491	molecular_function	oxidoreductase activity
G	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
G	0016618	molecular_function	hydroxypyruvate reductase [NAD(P)H] activity
G	0030267	molecular_function	glyoxylate reductase (NADPH) activity
G	0050609	molecular_function	phosphonate dehydrogenase activity
G	0051287	molecular_function	NAD binding
H	0005829	cellular_component	cytosol
H	0008465	molecular_function	hydroxypyruvate reductase (NADH) activity
H	0016491	molecular_function	oxidoreductase activity
H	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
H	0016618	molecular_function	hydroxypyruvate reductase [NAD(P)H] activity
H	0030267	molecular_function	glyoxylate reductase (NADPH) activity
H	0050609	molecular_function	phosphonate dehydrogenase activity
H	0051287	molecular_function	NAD binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	30
Details	BINDING SITE FOR RESIDUE NAD A 800

Chain	Residue
A	LYS76
A	ALA176
A	LEU208
A	PRO209
A	PRO235
A	CYS236
A	ARG237
A	ASP261
A	HIS292
A	GLY294
A	HOH905
A	THR104
A	HOH927
A	HOH937
A	HOH953
A	HOH954
A	HOH960
A	HOH1008
A	HOH1025
A	HOH1055
A	HOH1092
A	HOH1151
A	LEU151
A	HOH1244
A	GLY152
A	GLY154
A	ALA155
A	ILE156
A	HIS174
A	GLU175

site_id	AC2
Number of Residues	32
Details	BINDING SITE FOR RESIDUE NAD B 800

Chain	Residue
B	LYS76
B	GLY77
B	THR104
B	GLY152
B	GLY154
B	ALA155
B	ILE156
B	HIS174
B	GLU175
B	ALA176
B	ALA207
B	LEU208
B	PRO209
B	PRO235
B	CYS236
B	ARG237
B	ASP261
B	HIS292
B	GLY294
B	HOH902
B	HOH904
B	HOH910
B	HOH916
B	HOH958
B	HOH967
B	HOH979
B	HOH987
B	HOH1006
B	HOH1028
B	HOH1039
B	HOH1140
B	HOH1220

site_id	AC3
Number of Residues	28
Details	BINDING SITE FOR RESIDUE NAD C 800

Chain	Residue
C	LYS76
C	GLY77
C	THR104
C	GLY152
C	GLY154
C	ALA155
C	ILE156
C	HIS174
C	GLU175
C	ALA176
C	LYS177
C	ALA207
C	LEU208
C	PRO209
C	PRO235
C	CYS236
C	ARG237
C	ASP261
C	HIS292
C	GLY294
C	HOH911
C	HOH912
C	HOH919
C	HOH930
C	HOH971
C	HOH1038
C	HOH1066
C	HOH1078

site_id	AC4
Number of Residues	31
Details	BINDING SITE FOR RESIDUE NAD D 800

Chain	Residue
D	ALA176
D	ALA207
D	LEU208
D	PRO209
D	PRO235
D	CYS236
D	ARG237
D	ASP261
D	HIS292
D	GLY294
D	HOH904
D	HOH908
D	HOH929
D	HOH967
D	HOH977
D	HOH981
D	HOH1164
D	HOH1197
D	HOH1237
D	HOH1241
D	HOH1285
D	HOH1286
D	LYS76
D	GLY77
D	THR104
D	GLY152
D	GLY154
D	ALA155
D	ILE156
D	HIS174
D	GLU175

site_id	AC5
Number of Residues	31
Details	BINDING SITE FOR RESIDUE NAD E 800

Chain	Residue
E	LYS76
E	GLY77
E	THR104
E	GLY152
E	GLY154
E	ALA155
E	ILE156
E	HIS174
E	GLU175
E	ALA176
E	ALA207
E	LEU208
E	PRO209
E	PRO235
E	CYS236
E	ARG237
E	ASP261
E	HIS292
E	GLY294
E	HOH906
E	HOH908
E	HOH916
E	HOH929
E	HOH939
E	HOH1005
E	HOH1014
E	HOH1056
E	HOH1067
E	HOH1078
E	HOH1105
E	HOH1125

site_id	AC6
Number of Residues	36
Details	BINDING SITE FOR RESIDUE NAD F 800

Chain	Residue
F	LYS76
F	GLY77
F	THR104
F	LEU151
F	GLY152
F	GLY154
F	ALA155
F	ILE156
F	HIS174
F	GLU175
F	ALA176
F	LYS177
F	ALA207
F	LEU208
F	PRO209
F	LEU217
F	PRO235
F	CYS236
F	ARG237
F	ASP261
F	HIS292
F	GLY294
F	HOH902
F	HOH904
F	HOH919
F	HOH940
F	HOH947
F	HOH972
F	HOH1014
F	HOH1092
F	HOH1101
F	HOH1143
F	HOH1148
F	HOH1237
F	HOH1238
F	HOH1242

site_id	AC7
Number of Residues	33
Details	BINDING SITE FOR RESIDUE NAD G 800

Chain	Residue
G	LYS76
G	GLY77
G	THR104
G	LEU151
G	GLY152
G	GLY154
G	ALA155
G	ILE156
G	HIS174
G	GLU175
G	ALA176
G	LYS177
G	ALA207
G	LEU208
G	PRO209
G	PRO235
G	CYS236
G	ARG237
G	ASP261
G	HIS292
G	GLY294
G	HOH908
G	HOH915
G	HOH917
G	HOH934
G	HOH939
G	HOH972
G	HOH1036
G	HOH1044
G	HOH1082
G	HOH1106
G	HOH1226
G	HOH1239

site_id	AC8
Number of Residues	23
Details	BINDING SITE FOR RESIDUE NAD H 800

Chain	Residue
H	LYS76
H	GLY77
H	LEU100
H	THR104
H	GLY154
H	ALA155
H	ILE156
H	HIS174
H	GLU175
H	ALA176
H	ALA207
H	LEU208
H	PRO209
H	PRO235
H	CYS236
H	ARG237
H	ASP261
H	HIS292
H	GLY294
H	HOH910
H	HOH942
H	HOH954
H	HOH1023

Functional Information from PROSITE/UniProt

site_id	PS00671
Number of Residues	17
Details	D_2_HYDROXYACID_DH_3 D-isomer specific 2-hydroxyacid dehydrogenases signature 3. VRpGaLLVNpCRGsVVD

Chain	Residue	Details
A	VAL226-ASP242

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	16
Details	Active site: {"evidences":[{"evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

site_id	SWS_FT_FI2
Number of Residues	8
Details	Active site: {"description":"Proton donor","evidences":[{"evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

site_id	SWS_FT_FI3
Number of Residues	64
Details	Binding site: {"evidences":[{"evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)