4E5N
Thermostable phosphite dehydrogenase in complex with NAD
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0005829 | cellular_component | cytosol |
| A | 0008465 | molecular_function | hydroxypyruvate reductase (NADH) activity |
| A | 0016491 | molecular_function | oxidoreductase activity |
| A | 0016616 | molecular_function | oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor |
| A | 0016618 | molecular_function | hydroxypyruvate reductase [NAD(P)H] activity |
| A | 0030267 | molecular_function | glyoxylate reductase (NADPH) activity |
| A | 0050609 | molecular_function | phosphonate dehydrogenase activity |
| A | 0051287 | molecular_function | NAD binding |
| B | 0005829 | cellular_component | cytosol |
| B | 0008465 | molecular_function | hydroxypyruvate reductase (NADH) activity |
| B | 0016491 | molecular_function | oxidoreductase activity |
| B | 0016616 | molecular_function | oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor |
| B | 0016618 | molecular_function | hydroxypyruvate reductase [NAD(P)H] activity |
| B | 0030267 | molecular_function | glyoxylate reductase (NADPH) activity |
| B | 0050609 | molecular_function | phosphonate dehydrogenase activity |
| B | 0051287 | molecular_function | NAD binding |
| C | 0005829 | cellular_component | cytosol |
| C | 0008465 | molecular_function | hydroxypyruvate reductase (NADH) activity |
| C | 0016491 | molecular_function | oxidoreductase activity |
| C | 0016616 | molecular_function | oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor |
| C | 0016618 | molecular_function | hydroxypyruvate reductase [NAD(P)H] activity |
| C | 0030267 | molecular_function | glyoxylate reductase (NADPH) activity |
| C | 0050609 | molecular_function | phosphonate dehydrogenase activity |
| C | 0051287 | molecular_function | NAD binding |
| D | 0005829 | cellular_component | cytosol |
| D | 0008465 | molecular_function | hydroxypyruvate reductase (NADH) activity |
| D | 0016491 | molecular_function | oxidoreductase activity |
| D | 0016616 | molecular_function | oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor |
| D | 0016618 | molecular_function | hydroxypyruvate reductase [NAD(P)H] activity |
| D | 0030267 | molecular_function | glyoxylate reductase (NADPH) activity |
| D | 0050609 | molecular_function | phosphonate dehydrogenase activity |
| D | 0051287 | molecular_function | NAD binding |
| E | 0005829 | cellular_component | cytosol |
| E | 0008465 | molecular_function | hydroxypyruvate reductase (NADH) activity |
| E | 0016491 | molecular_function | oxidoreductase activity |
| E | 0016616 | molecular_function | oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor |
| E | 0016618 | molecular_function | hydroxypyruvate reductase [NAD(P)H] activity |
| E | 0030267 | molecular_function | glyoxylate reductase (NADPH) activity |
| E | 0050609 | molecular_function | phosphonate dehydrogenase activity |
| E | 0051287 | molecular_function | NAD binding |
| F | 0005829 | cellular_component | cytosol |
| F | 0008465 | molecular_function | hydroxypyruvate reductase (NADH) activity |
| F | 0016491 | molecular_function | oxidoreductase activity |
| F | 0016616 | molecular_function | oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor |
| F | 0016618 | molecular_function | hydroxypyruvate reductase [NAD(P)H] activity |
| F | 0030267 | molecular_function | glyoxylate reductase (NADPH) activity |
| F | 0050609 | molecular_function | phosphonate dehydrogenase activity |
| F | 0051287 | molecular_function | NAD binding |
| G | 0005829 | cellular_component | cytosol |
| G | 0008465 | molecular_function | hydroxypyruvate reductase (NADH) activity |
| G | 0016491 | molecular_function | oxidoreductase activity |
| G | 0016616 | molecular_function | oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor |
| G | 0016618 | molecular_function | hydroxypyruvate reductase [NAD(P)H] activity |
| G | 0030267 | molecular_function | glyoxylate reductase (NADPH) activity |
| G | 0050609 | molecular_function | phosphonate dehydrogenase activity |
| G | 0051287 | molecular_function | NAD binding |
| H | 0005829 | cellular_component | cytosol |
| H | 0008465 | molecular_function | hydroxypyruvate reductase (NADH) activity |
| H | 0016491 | molecular_function | oxidoreductase activity |
| H | 0016616 | molecular_function | oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor |
| H | 0016618 | molecular_function | hydroxypyruvate reductase [NAD(P)H] activity |
| H | 0030267 | molecular_function | glyoxylate reductase (NADPH) activity |
| H | 0050609 | molecular_function | phosphonate dehydrogenase activity |
| H | 0051287 | molecular_function | NAD binding |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 30 |
| Details | BINDING SITE FOR RESIDUE NAD A 800 |
| Chain | Residue |
| A | LYS76 |
| A | ALA176 |
| A | LEU208 |
| A | PRO209 |
| A | PRO235 |
| A | CYS236 |
| A | ARG237 |
| A | ASP261 |
| A | HIS292 |
| A | GLY294 |
| A | HOH905 |
| A | THR104 |
| A | HOH927 |
| A | HOH937 |
| A | HOH953 |
| A | HOH954 |
| A | HOH960 |
| A | HOH1008 |
| A | HOH1025 |
| A | HOH1055 |
| A | HOH1092 |
| A | HOH1151 |
| A | LEU151 |
| A | HOH1244 |
| A | GLY152 |
| A | GLY154 |
| A | ALA155 |
| A | ILE156 |
| A | HIS174 |
| A | GLU175 |
| site_id | AC2 |
| Number of Residues | 32 |
| Details | BINDING SITE FOR RESIDUE NAD B 800 |
| Chain | Residue |
| B | LYS76 |
| B | GLY77 |
| B | THR104 |
| B | GLY152 |
| B | GLY154 |
| B | ALA155 |
| B | ILE156 |
| B | HIS174 |
| B | GLU175 |
| B | ALA176 |
| B | ALA207 |
| B | LEU208 |
| B | PRO209 |
| B | PRO235 |
| B | CYS236 |
| B | ARG237 |
| B | ASP261 |
| B | HIS292 |
| B | GLY294 |
| B | HOH902 |
| B | HOH904 |
| B | HOH910 |
| B | HOH916 |
| B | HOH958 |
| B | HOH967 |
| B | HOH979 |
| B | HOH987 |
| B | HOH1006 |
| B | HOH1028 |
| B | HOH1039 |
| B | HOH1140 |
| B | HOH1220 |
| site_id | AC3 |
| Number of Residues | 28 |
| Details | BINDING SITE FOR RESIDUE NAD C 800 |
| Chain | Residue |
| C | LYS76 |
| C | GLY77 |
| C | THR104 |
| C | GLY152 |
| C | GLY154 |
| C | ALA155 |
| C | ILE156 |
| C | HIS174 |
| C | GLU175 |
| C | ALA176 |
| C | LYS177 |
| C | ALA207 |
| C | LEU208 |
| C | PRO209 |
| C | PRO235 |
| C | CYS236 |
| C | ARG237 |
| C | ASP261 |
| C | HIS292 |
| C | GLY294 |
| C | HOH911 |
| C | HOH912 |
| C | HOH919 |
| C | HOH930 |
| C | HOH971 |
| C | HOH1038 |
| C | HOH1066 |
| C | HOH1078 |
| site_id | AC4 |
| Number of Residues | 31 |
| Details | BINDING SITE FOR RESIDUE NAD D 800 |
| Chain | Residue |
| D | ALA176 |
| D | ALA207 |
| D | LEU208 |
| D | PRO209 |
| D | PRO235 |
| D | CYS236 |
| D | ARG237 |
| D | ASP261 |
| D | HIS292 |
| D | GLY294 |
| D | HOH904 |
| D | HOH908 |
| D | HOH929 |
| D | HOH967 |
| D | HOH977 |
| D | HOH981 |
| D | HOH1164 |
| D | HOH1197 |
| D | HOH1237 |
| D | HOH1241 |
| D | HOH1285 |
| D | HOH1286 |
| D | LYS76 |
| D | GLY77 |
| D | THR104 |
| D | GLY152 |
| D | GLY154 |
| D | ALA155 |
| D | ILE156 |
| D | HIS174 |
| D | GLU175 |
| site_id | AC5 |
| Number of Residues | 31 |
| Details | BINDING SITE FOR RESIDUE NAD E 800 |
| Chain | Residue |
| E | LYS76 |
| E | GLY77 |
| E | THR104 |
| E | GLY152 |
| E | GLY154 |
| E | ALA155 |
| E | ILE156 |
| E | HIS174 |
| E | GLU175 |
| E | ALA176 |
| E | ALA207 |
| E | LEU208 |
| E | PRO209 |
| E | PRO235 |
| E | CYS236 |
| E | ARG237 |
| E | ASP261 |
| E | HIS292 |
| E | GLY294 |
| E | HOH906 |
| E | HOH908 |
| E | HOH916 |
| E | HOH929 |
| E | HOH939 |
| E | HOH1005 |
| E | HOH1014 |
| E | HOH1056 |
| E | HOH1067 |
| E | HOH1078 |
| E | HOH1105 |
| E | HOH1125 |
| site_id | AC6 |
| Number of Residues | 36 |
| Details | BINDING SITE FOR RESIDUE NAD F 800 |
| Chain | Residue |
| F | LYS76 |
| F | GLY77 |
| F | THR104 |
| F | LEU151 |
| F | GLY152 |
| F | GLY154 |
| F | ALA155 |
| F | ILE156 |
| F | HIS174 |
| F | GLU175 |
| F | ALA176 |
| F | LYS177 |
| F | ALA207 |
| F | LEU208 |
| F | PRO209 |
| F | LEU217 |
| F | PRO235 |
| F | CYS236 |
| F | ARG237 |
| F | ASP261 |
| F | HIS292 |
| F | GLY294 |
| F | HOH902 |
| F | HOH904 |
| F | HOH919 |
| F | HOH940 |
| F | HOH947 |
| F | HOH972 |
| F | HOH1014 |
| F | HOH1092 |
| F | HOH1101 |
| F | HOH1143 |
| F | HOH1148 |
| F | HOH1237 |
| F | HOH1238 |
| F | HOH1242 |
| site_id | AC7 |
| Number of Residues | 33 |
| Details | BINDING SITE FOR RESIDUE NAD G 800 |
| Chain | Residue |
| G | LYS76 |
| G | GLY77 |
| G | THR104 |
| G | LEU151 |
| G | GLY152 |
| G | GLY154 |
| G | ALA155 |
| G | ILE156 |
| G | HIS174 |
| G | GLU175 |
| G | ALA176 |
| G | LYS177 |
| G | ALA207 |
| G | LEU208 |
| G | PRO209 |
| G | PRO235 |
| G | CYS236 |
| G | ARG237 |
| G | ASP261 |
| G | HIS292 |
| G | GLY294 |
| G | HOH908 |
| G | HOH915 |
| G | HOH917 |
| G | HOH934 |
| G | HOH939 |
| G | HOH972 |
| G | HOH1036 |
| G | HOH1044 |
| G | HOH1082 |
| G | HOH1106 |
| G | HOH1226 |
| G | HOH1239 |
| site_id | AC8 |
| Number of Residues | 23 |
| Details | BINDING SITE FOR RESIDUE NAD H 800 |
| Chain | Residue |
| H | LYS76 |
| H | GLY77 |
| H | LEU100 |
| H | THR104 |
| H | GLY154 |
| H | ALA155 |
| H | ILE156 |
| H | HIS174 |
| H | GLU175 |
| H | ALA176 |
| H | ALA207 |
| H | LEU208 |
| H | PRO209 |
| H | PRO235 |
| H | CYS236 |
| H | ARG237 |
| H | ASP261 |
| H | HIS292 |
| H | GLY294 |
| H | HOH910 |
| H | HOH942 |
| H | HOH954 |
| H | HOH1023 |
Functional Information from PROSITE/UniProt
| site_id | PS00671 |
| Number of Residues | 17 |
| Details | D_2_HYDROXYACID_DH_3 D-isomer specific 2-hydroxyacid dehydrogenases signature 3. VRpGaLLVNpCRGsVVD |
| Chain | Residue | Details |
| A | VAL226-ASP242 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 16 |
| Details | ACT_SITE: ACT_SITE => ECO:0000250 |
| Chain | Residue | Details |
| A | ARG237 | |
| E | GLU266 | |
| F | ARG237 | |
| F | GLU266 | |
| G | ARG237 | |
| G | GLU266 | |
| H | ARG237 | |
| H | GLU266 | |
| A | GLU266 | |
| B | ARG237 | |
| B | GLU266 | |
| C | ARG237 | |
| C | GLU266 | |
| D | ARG237 | |
| D | GLU266 | |
| E | ARG237 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 8 |
| Details | ACT_SITE: Proton donor => ECO:0000250 |
| Chain | Residue | Details |
| A | HIS292 | |
| B | HIS292 | |
| C | HIS292 | |
| D | HIS292 | |
| E | HIS292 | |
| F | HIS292 | |
| G | HIS292 | |
| H | HIS292 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 40 |
| Details | BINDING: BINDING => ECO:0000250 |
| Chain | Residue | Details |
| A | ALA155 | |
| B | HIS292 | |
| C | ALA155 | |
| C | GLU175 | |
| C | PRO235 | |
| C | ASP261 | |
| C | HIS292 | |
| D | ALA155 | |
| D | GLU175 | |
| D | PRO235 | |
| D | ASP261 | |
| A | GLU175 | |
| D | HIS292 | |
| E | ALA155 | |
| E | GLU175 | |
| E | PRO235 | |
| E | ASP261 | |
| E | HIS292 | |
| F | ALA155 | |
| F | GLU175 | |
| F | PRO235 | |
| F | ASP261 | |
| A | PRO235 | |
| F | HIS292 | |
| G | ALA155 | |
| G | GLU175 | |
| G | PRO235 | |
| G | ASP261 | |
| G | HIS292 | |
| H | ALA155 | |
| H | GLU175 | |
| H | PRO235 | |
| H | ASP261 | |
| A | ASP261 | |
| H | HIS292 | |
| A | HIS292 | |
| B | ALA155 | |
| B | GLU175 | |
| B | PRO235 | |
| B | ASP261 |
