4DSN
Small-molecule ligands bind to a distinct pocket in Ras and inhibit SOS-mediated nucleotide exchange activity
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0000139 | cellular_component | Golgi membrane |
| A | 0000165 | biological_process | MAPK cascade |
| A | 0000166 | molecular_function | nucleotide binding |
| A | 0001889 | biological_process | liver development |
| A | 0003924 | molecular_function | GTPase activity |
| A | 0003925 | molecular_function | G protein activity |
| A | 0005515 | molecular_function | protein binding |
| A | 0005525 | molecular_function | GTP binding |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0005741 | cellular_component | mitochondrial outer membrane |
| A | 0005789 | cellular_component | endoplasmic reticulum membrane |
| A | 0005829 | cellular_component | cytosol |
| A | 0005886 | cellular_component | plasma membrane |
| A | 0005925 | cellular_component | focal adhesion |
| A | 0007165 | biological_process | signal transduction |
| A | 0007265 | biological_process | Ras protein signal transduction |
| A | 0007565 | biological_process | female pregnancy |
| A | 0008283 | biological_process | cell population proliferation |
| A | 0008542 | biological_process | visual learning |
| A | 0009629 | biological_process | response to gravity |
| A | 0009898 | cellular_component | cytoplasmic side of plasma membrane |
| A | 0010467 | biological_process | gene expression |
| A | 0010628 | biological_process | positive regulation of gene expression |
| A | 0012505 | cellular_component | endomembrane system |
| A | 0014009 | biological_process | glial cell proliferation |
| A | 0016020 | cellular_component | membrane |
| A | 0016601 | biological_process | Rac protein signal transduction |
| A | 0016787 | molecular_function | hydrolase activity |
| A | 0019002 | molecular_function | GMP binding |
| A | 0019003 | molecular_function | GDP binding |
| A | 0019221 | biological_process | cytokine-mediated signaling pathway |
| A | 0021897 | biological_process | forebrain astrocyte development |
| A | 0030036 | biological_process | actin cytoskeleton organization |
| A | 0030275 | molecular_function | LRR domain binding |
| A | 0030857 | biological_process | negative regulation of epithelial cell differentiation |
| A | 0032228 | biological_process | regulation of synaptic transmission, GABAergic |
| A | 0035022 | biological_process | positive regulation of Rac protein signal transduction |
| A | 0035900 | biological_process | response to isolation stress |
| A | 0035914 | biological_process | skeletal muscle cell differentiation |
| A | 0043495 | molecular_function | membrane-membrane adaptor activity |
| A | 0043524 | biological_process | negative regulation of neuron apoptotic process |
| A | 0044877 | molecular_function | protein-containing complex binding |
| A | 0048169 | biological_process | regulation of long-term neuronal synaptic plasticity |
| A | 0048873 | biological_process | homeostasis of number of cells within a tissue |
| A | 0051146 | biological_process | striated muscle cell differentiation |
| A | 0051384 | biological_process | response to glucocorticoid |
| A | 0051385 | biological_process | response to mineralocorticoid |
| A | 0051402 | biological_process | neuron apoptotic process |
| A | 0051450 | biological_process | myoblast proliferation |
| A | 0060038 | biological_process | cardiac muscle cell proliferation |
| A | 0060252 | biological_process | positive regulation of glial cell proliferation |
| A | 0060441 | biological_process | epithelial tube branching involved in lung morphogenesis |
| A | 0060509 | biological_process | type I pneumocyte differentiation |
| A | 2000774 | biological_process | positive regulation of cellular senescence |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 30 |
| Details | BINDING SITE FOR RESIDUE GCP A 201 |
| Chain | Residue |
| A | ASP12 |
| A | ASP30 |
| A | GLU31 |
| A | TYR32 |
| A | PRO34 |
| A | THR35 |
| A | GLY60 |
| A | ASN116 |
| A | LYS117 |
| A | ASP119 |
| A | LEU120 |
| A | GLY13 |
| A | SER145 |
| A | ALA146 |
| A | LYS147 |
| A | MG202 |
| A | HOH301 |
| A | HOH302 |
| A | HOH303 |
| A | HOH309 |
| A | HOH319 |
| A | HOH333 |
| A | VAL14 |
| A | HOH336 |
| A | GLY15 |
| A | LYS16 |
| A | SER17 |
| A | ALA18 |
| A | PHE28 |
| A | VAL29 |
| site_id | AC2 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE MG A 202 |
| Chain | Residue |
| A | SER17 |
| A | THR35 |
| A | GCP201 |
| A | HOH301 |
| A | HOH302 |
| site_id | AC3 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE MG A 203 |
| Chain | Residue |
| A | GLY10 |
| A | ALA59 |
| A | GLY60 |
| A | TYR96 |
| A | HOH305 |
| A | HOH365 |
| site_id | AC4 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE EDO A 204 |
| Chain | Residue |
| A | LYS101 |
| A | ASP105 |
| A | SER106 |
| A | GLU107 |
| site_id | AC5 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE EDO A 205 |
| Chain | Residue |
| A | THR35 |
| A | ILE36 |
| A | GLU37 |
| A | ASP38 |
| A | THR148 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 4 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:22431598, ECO:0000269|PubMed:22566140, ECO:0000305|PubMed:34380736, ECO:0000305|PubMed:35522713 |
| Chain | Residue | Details |
| A | GLY10 | |
| A | VAL29 | |
| A | ALA59 | |
| A | ASN116 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 1 |
| Details | MOD_RES: N-acetylthreonine; in GTPase KRas, N-terminally processed => ECO:0000269|Ref.17 |
| Chain | Residue | Details |
| A | THR2 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 1 |
| Details | MOD_RES: N6-acetyllysine => ECO:0000269|PubMed:22711838 |
| Chain | Residue | Details |
| A | LYS104 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 1 |
| Details | MOD_RES: Cysteine methyl ester => ECO:0000269|PubMed:27791178, ECO:0007744|PDB:5TAR, ECO:0007744|PDB:5TB5 |
| Chain | Residue | Details |
| A | VAL186 |
| site_id | SWS_FT_FI5 |
| Number of Residues | 1 |
| Details | LIPID: S-palmitoyl cysteine => ECO:0000305|PubMed:29239724 |
| Chain | Residue | Details |
| A | LYS180 |
| site_id | SWS_FT_FI6 |
| Number of Residues | 1 |
| Details | LIPID: N6-palmitoyl lysine => ECO:0000269|PubMed:29239724 |
| Chain | Residue | Details |
| A | LYS182 |
| site_id | SWS_FT_FI7 |
| Number of Residues | 2 |
| Details | LIPID: N6-palmitoyl lysine => ECO:0000305|PubMed:29239724 |
| Chain | Residue | Details |
| A | LYS184 | |
| A | CYS185 |
| site_id | SWS_FT_FI8 |
| Number of Residues | 1 |
| Details | LIPID: S-farnesyl cysteine => ECO:0000269|PubMed:27791178, ECO:0000305|PubMed:24415755, ECO:0007744|PDB:5TAR, ECO:0007744|PDB:5TB5 |
| Chain | Residue | Details |
| A | VAL186 |
| site_id | SWS_FT_FI9 |
| Number of Residues | 1 |
| Details | CARBOHYD: (Microbial infection) O-linked (Glc) threonine; by P.sordellii toxin TcsL => ECO:0000269|PubMed:19744486 |
| Chain | Residue | Details |
| A | THR35 |
| site_id | SWS_FT_FI10 |
| Number of Residues | 2 |
| Details | CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000305|PubMed:30442762 |
| Chain | Residue | Details |
| A | MET170 |
