4CZU
Crystal structure of the kinase domain of CIPK23 T190D mutant
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0004672 | molecular_function | protein kinase activity |
| A | 0004674 | molecular_function | protein serine/threonine kinase activity |
| A | 0005515 | molecular_function | protein binding |
| A | 0005524 | molecular_function | ATP binding |
| A | 0005634 | cellular_component | nucleus |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0005829 | cellular_component | cytosol |
| A | 0005886 | cellular_component | plasma membrane |
| A | 0006468 | biological_process | protein phosphorylation |
| A | 0007165 | biological_process | signal transduction |
| A | 0007584 | biological_process | response to nutrient |
| A | 0009414 | biological_process | response to water deprivation |
| A | 0009507 | cellular_component | chloroplast |
| A | 0009536 | cellular_component | plastid |
| A | 0010118 | biological_process | stomatal movement |
| A | 0010119 | biological_process | regulation of stomatal movement |
| A | 0106310 | molecular_function | protein serine kinase activity |
| A | 1990573 | biological_process | potassium ion import across plasma membrane |
| B | 0004672 | molecular_function | protein kinase activity |
| B | 0004674 | molecular_function | protein serine/threonine kinase activity |
| B | 0005515 | molecular_function | protein binding |
| B | 0005524 | molecular_function | ATP binding |
| B | 0005634 | cellular_component | nucleus |
| B | 0005737 | cellular_component | cytoplasm |
| B | 0005829 | cellular_component | cytosol |
| B | 0005886 | cellular_component | plasma membrane |
| B | 0006468 | biological_process | protein phosphorylation |
| B | 0007165 | biological_process | signal transduction |
| B | 0007584 | biological_process | response to nutrient |
| B | 0009414 | biological_process | response to water deprivation |
| B | 0009507 | cellular_component | chloroplast |
| B | 0009536 | cellular_component | plastid |
| B | 0010118 | biological_process | stomatal movement |
| B | 0010119 | biological_process | regulation of stomatal movement |
| B | 0106310 | molecular_function | protein serine kinase activity |
| B | 1990573 | biological_process | potassium ion import across plasma membrane |
| C | 0004672 | molecular_function | protein kinase activity |
| C | 0004674 | molecular_function | protein serine/threonine kinase activity |
| C | 0005515 | molecular_function | protein binding |
| C | 0005524 | molecular_function | ATP binding |
| C | 0005634 | cellular_component | nucleus |
| C | 0005737 | cellular_component | cytoplasm |
| C | 0005829 | cellular_component | cytosol |
| C | 0005886 | cellular_component | plasma membrane |
| C | 0006468 | biological_process | protein phosphorylation |
| C | 0007165 | biological_process | signal transduction |
| C | 0007584 | biological_process | response to nutrient |
| C | 0009414 | biological_process | response to water deprivation |
| C | 0009507 | cellular_component | chloroplast |
| C | 0009536 | cellular_component | plastid |
| C | 0010118 | biological_process | stomatal movement |
| C | 0010119 | biological_process | regulation of stomatal movement |
| C | 0106310 | molecular_function | protein serine kinase activity |
| C | 1990573 | biological_process | potassium ion import across plasma membrane |
| D | 0004672 | molecular_function | protein kinase activity |
| D | 0004674 | molecular_function | protein serine/threonine kinase activity |
| D | 0005515 | molecular_function | protein binding |
| D | 0005524 | molecular_function | ATP binding |
| D | 0005634 | cellular_component | nucleus |
| D | 0005737 | cellular_component | cytoplasm |
| D | 0005829 | cellular_component | cytosol |
| D | 0005886 | cellular_component | plasma membrane |
| D | 0006468 | biological_process | protein phosphorylation |
| D | 0007165 | biological_process | signal transduction |
| D | 0007584 | biological_process | response to nutrient |
| D | 0009414 | biological_process | response to water deprivation |
| D | 0009507 | cellular_component | chloroplast |
| D | 0009536 | cellular_component | plastid |
| D | 0010118 | biological_process | stomatal movement |
| D | 0010119 | biological_process | regulation of stomatal movement |
| D | 0106310 | molecular_function | protein serine kinase activity |
| D | 1990573 | biological_process | potassium ion import across plasma membrane |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 11 |
| Details | BINDING SITE FOR RESIDUE CPS A 1321 |
| Chain | Residue |
| A | LEU37 |
| A | HOH2146 |
| C | SER122 |
| A | VAL45 |
| A | GLU109 |
| A | THR112 |
| A | LEU161 |
| A | ASP172 |
| A | LEU175 |
| A | HOH2104 |
| A | HOH2141 |
| site_id | AC2 |
| Number of Residues | 10 |
| Details | BINDING SITE FOR RESIDUE CPS B 1314 |
| Chain | Residue |
| B | VAL45 |
| B | ALA58 |
| B | GLU109 |
| B | GLY114 |
| B | GLU115 |
| B | SER171 |
| B | ASP172 |
| B | HOH2091 |
| B | HOH2127 |
| B | HOH2133 |
| site_id | AC3 |
| Number of Residues | 11 |
| Details | BINDING SITE FOR RESIDUE CPS C 1313 |
| Chain | Residue |
| A | SER121 |
| A | HOH2113 |
| C | ALA58 |
| C | GLU109 |
| C | LEU161 |
| C | SER171 |
| C | ASP172 |
| C | LEU175 |
| C | HOH2053 |
| C | HOH2090 |
| C | HOH2096 |
| site_id | AC4 |
| Number of Residues | 12 |
| Details | BINDING SITE FOR RESIDUE CPS D 1325 |
| Chain | Residue |
| B | GLU52 |
| B | SER121 |
| B | SER122 |
| D | ALA58 |
| D | GLU109 |
| D | VAL111 |
| D | LEU161 |
| D | SER171 |
| D | ASP172 |
| D | HIS189 |
| D | HOH2097 |
| D | HOH2174 |
| site_id | AC5 |
| Number of Residues | 11 |
| Details | BINDING SITE FOR RESIDUE CPS C 1314 |
| Chain | Residue |
| A | ILE203 |
| A | ASN204 |
| A | ASN236 |
| A | THR238 |
| C | ILE203 |
| C | ASN204 |
| C | LYS206 |
| C | LEU237 |
| C | TYR241 |
| C | HOH2162 |
| C | HOH2163 |
| site_id | AC6 |
| Number of Residues | 9 |
| Details | BINDING SITE FOR RESIDUE CPS B 1315 |
| Chain | Residue |
| B | ILE203 |
| B | THR238 |
| B | TYR241 |
| B | HOH2232 |
| B | HOH2233 |
| B | HOH2234 |
| D | ASN204 |
| D | THR238 |
| D | CPS1326 |
| site_id | AC7 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE CPS D 1326 |
| Chain | Residue |
| B | CPS1315 |
| D | TYR241 |
| D | LYS242 |
| D | LYS246 |
| D | HOH2130 |
| site_id | AC8 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE SO4 D 1327 |
| Chain | Residue |
| D | VAL111 |
| D | THR112 |
| D | ASP163 |
| D | ALA164 |
| site_id | AC9 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE SO4 C 1315 |
| Chain | Residue |
| C | VAL111 |
| C | THR112 |
| C | ASP163 |
| C | ALA164 |
| C | HOH2012 |
| site_id | BC1 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE SO4 C 1316 |
| Chain | Residue |
| C | ALA257 |
| C | SER258 |
| C | HOH2141 |
| C | HOH2165 |
| D | LYS260 |
| site_id | BC2 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE SO4 B 1316 |
| Chain | Residue |
| A | ARG93 |
| B | SER256 |
| B | ALA257 |
| B | SER258 |
| B | HOH2199 |
| site_id | BC3 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE SO4 A 1322 |
| Chain | Residue |
| A | SER256 |
| A | ALA257 |
| A | SER258 |
| A | HOH2210 |
| site_id | BC4 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE SO4 B 1317 |
| Chain | Residue |
| B | ARG93 |
| D | PRO19 |
| D | HOH2001 |
| site_id | BC5 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE SO4 C 1317 |
| Chain | Residue |
| C | GLU282 |
| C | ASN283 |
| C | GLU284 |
| C | HOH2157 |
| site_id | BC6 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE SO4 A 1323 |
| Chain | Residue |
| A | VAL111 |
| A | THR112 |
| A | ASP163 |
| A | ALA164 |
| A | HOH2144 |
| B | LYS288 |
| B | GLY289 |
| site_id | BC7 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE SO4 A 1324 |
| Chain | Residue |
| A | LYS64 |
| A | ASP311 |
| site_id | BC8 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE SO4 B 1318 |
| Chain | Residue |
| B | ASN70 |
| B | PRO179 |
| B | GLN180 |
| site_id | BC9 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE SO4 D 1328 |
| Chain | Residue |
| A | GLY20 |
| A | ILE21 |
| D | ARG25 |
| D | ARG35 |
| D | THR36 |
| D | HOH2007 |
| site_id | CC1 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE SO4 D 1329 |
| Chain | Residue |
| D | LYS64 |
| D | ASP310 |
| D | ASP311 |
| site_id | CC2 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE SO4 B 1319 |
| Chain | Residue |
| B | LYS64 |
| B | ASP310 |
| B | ASP311 |
| B | HOH2078 |
| site_id | CC3 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE SO4 C 1318 |
| Chain | Residue |
| C | LYS64 |
| C | ASP311 |
| site_id | CC4 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE SO4 C 1319 |
| Chain | Residue |
| C | ASN70 |
| C | PRO179 |
| C | GLN180 |
| C | HOH2101 |
| site_id | CC5 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE SO4 A 1325 |
| Chain | Residue |
| A | SER235 |
| A | ASN236 |
| A | HOH2245 |
| C | TYR208 |
| site_id | CC6 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE SO4 D 1330 |
| Chain | Residue |
| D | SER235 |
| D | ASN236 |
| D | HOH2122 |
| D | HOH2175 |
| D | HOH2176 |
Functional Information from PROSITE/UniProt
| site_id | PS00107 |
| Number of Residues | 24 |
| Details | PROTEIN_KINASE_ATP Protein kinases ATP-binding region signature. LGEGTFAKVKfArnvengdn..........VAIK |
| Chain | Residue | Details |
| A | LEU37-LYS60 |
| site_id | PS00108 |
| Number of Residues | 13 |
| Details | PROTEIN_KINASE_ST Serine/Threonine protein kinases active-site signature. VyHrDLKpeNLLL |
| Chain | Residue | Details |
| A | VAL150-LEU162 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 1020 |
| Details | Domain: {"description":"Protein kinase","evidences":[{"source":"PROSITE-ProRule","id":"PRU00159","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 116 |
| Details | Region: {"description":"Activation loop","evidences":[{"evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI3 |
| Number of Residues | 4 |
| Details | Active site: {"description":"Proton acceptor","evidences":[{"source":"PROSITE-ProRule","id":"PRU00159","evidenceCode":"ECO:0000255"},{"source":"PROSITE-ProRule","id":"PRU10027","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI4 |
| Number of Residues | 36 |
| Details | Binding site: {"evidences":[{"source":"PROSITE-ProRule","id":"PRU00159","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI5 |
| Number of Residues | 4 |
| Details | Modified residue: {"description":"Phosphoserine","evidences":[{"source":"UniProtKB","id":"Q93V58","evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI6 |
| Number of Residues | 4 |
| Details | Modified residue: {"description":"Phosphothreonine","evidences":[{"source":"UniProtKB","id":"Q38997","evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
