4CZT
Crystal structure of the kinase domain of CIPK23
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0004672 | molecular_function | protein kinase activity |
| A | 0004674 | molecular_function | protein serine/threonine kinase activity |
| A | 0005267 | molecular_function | potassium channel activity |
| A | 0005515 | molecular_function | protein binding |
| A | 0005524 | molecular_function | ATP binding |
| A | 0005634 | cellular_component | nucleus |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0005829 | cellular_component | cytosol |
| A | 0005886 | cellular_component | plasma membrane |
| A | 0006468 | biological_process | protein phosphorylation |
| A | 0007165 | biological_process | signal transduction |
| A | 0007584 | biological_process | response to nutrient |
| A | 0009414 | biological_process | response to water deprivation |
| A | 0009536 | cellular_component | plastid |
| A | 0010118 | biological_process | stomatal movement |
| A | 0010119 | biological_process | regulation of stomatal movement |
| A | 0071805 | biological_process | potassium ion transmembrane transport |
| A | 0106310 | molecular_function | protein serine kinase activity |
| A | 1990573 | biological_process | potassium ion import across plasma membrane |
| B | 0004672 | molecular_function | protein kinase activity |
| B | 0004674 | molecular_function | protein serine/threonine kinase activity |
| B | 0005267 | molecular_function | potassium channel activity |
| B | 0005515 | molecular_function | protein binding |
| B | 0005524 | molecular_function | ATP binding |
| B | 0005634 | cellular_component | nucleus |
| B | 0005737 | cellular_component | cytoplasm |
| B | 0005829 | cellular_component | cytosol |
| B | 0005886 | cellular_component | plasma membrane |
| B | 0006468 | biological_process | protein phosphorylation |
| B | 0007165 | biological_process | signal transduction |
| B | 0007584 | biological_process | response to nutrient |
| B | 0009414 | biological_process | response to water deprivation |
| B | 0009536 | cellular_component | plastid |
| B | 0010118 | biological_process | stomatal movement |
| B | 0010119 | biological_process | regulation of stomatal movement |
| B | 0071805 | biological_process | potassium ion transmembrane transport |
| B | 0106310 | molecular_function | protein serine kinase activity |
| B | 1990573 | biological_process | potassium ion import across plasma membrane |
| C | 0004672 | molecular_function | protein kinase activity |
| C | 0004674 | molecular_function | protein serine/threonine kinase activity |
| C | 0005267 | molecular_function | potassium channel activity |
| C | 0005515 | molecular_function | protein binding |
| C | 0005524 | molecular_function | ATP binding |
| C | 0005634 | cellular_component | nucleus |
| C | 0005737 | cellular_component | cytoplasm |
| C | 0005829 | cellular_component | cytosol |
| C | 0005886 | cellular_component | plasma membrane |
| C | 0006468 | biological_process | protein phosphorylation |
| C | 0007165 | biological_process | signal transduction |
| C | 0007584 | biological_process | response to nutrient |
| C | 0009414 | biological_process | response to water deprivation |
| C | 0009536 | cellular_component | plastid |
| C | 0010118 | biological_process | stomatal movement |
| C | 0010119 | biological_process | regulation of stomatal movement |
| C | 0071805 | biological_process | potassium ion transmembrane transport |
| C | 0106310 | molecular_function | protein serine kinase activity |
| C | 1990573 | biological_process | potassium ion import across plasma membrane |
| D | 0004672 | molecular_function | protein kinase activity |
| D | 0004674 | molecular_function | protein serine/threonine kinase activity |
| D | 0005267 | molecular_function | potassium channel activity |
| D | 0005515 | molecular_function | protein binding |
| D | 0005524 | molecular_function | ATP binding |
| D | 0005634 | cellular_component | nucleus |
| D | 0005737 | cellular_component | cytoplasm |
| D | 0005829 | cellular_component | cytosol |
| D | 0005886 | cellular_component | plasma membrane |
| D | 0006468 | biological_process | protein phosphorylation |
| D | 0007165 | biological_process | signal transduction |
| D | 0007584 | biological_process | response to nutrient |
| D | 0009414 | biological_process | response to water deprivation |
| D | 0009536 | cellular_component | plastid |
| D | 0010118 | biological_process | stomatal movement |
| D | 0010119 | biological_process | regulation of stomatal movement |
| D | 0071805 | biological_process | potassium ion transmembrane transport |
| D | 0106310 | molecular_function | protein serine kinase activity |
| D | 1990573 | biological_process | potassium ion import across plasma membrane |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 16 |
| Details | BINDING SITE FOR RESIDUE CPS A 1316 |
| Chain | Residue |
| A | ALA58 |
| A | ASP172 |
| A | HIS189 |
| A | HOH2051 |
| A | HOH2060 |
| A | HOH2061 |
| C | SER121 |
| C | CPS1314 |
| A | LEU108 |
| A | GLU109 |
| A | PHE110 |
| A | THR112 |
| A | GLY113 |
| A | GLY114 |
| A | LEU161 |
| A | SER171 |
| site_id | AC2 |
| Number of Residues | 12 |
| Details | BINDING SITE FOR RESIDUE CPS B 1314 |
| Chain | Residue |
| B | VAL45 |
| B | GLU109 |
| B | PHE110 |
| B | GLY113 |
| B | SER171 |
| B | ASP172 |
| B | HOH2063 |
| B | HOH2067 |
| B | HOH2104 |
| B | HOH2107 |
| D | SER121 |
| D | CPS1325 |
| site_id | AC3 |
| Number of Residues | 16 |
| Details | BINDING SITE FOR RESIDUE CPS C 1314 |
| Chain | Residue |
| A | GLU115 |
| A | ASP118 |
| A | SER121 |
| A | CPS1316 |
| A | HOH2063 |
| C | GLU109 |
| C | GLY114 |
| C | ASP118 |
| C | LEU161 |
| C | SER171 |
| C | ASP172 |
| C | LEU175 |
| C | LEU187 |
| C | HOH2033 |
| C | HOH2062 |
| C | HOH2065 |
| site_id | AC4 |
| Number of Residues | 13 |
| Details | BINDING SITE FOR RESIDUE CPS D 1325 |
| Chain | Residue |
| B | CPS1314 |
| D | LEU108 |
| D | GLU109 |
| D | VAL111 |
| D | THR112 |
| D | GLY113 |
| D | GLY114 |
| D | GLU115 |
| D | LEU161 |
| D | SER171 |
| D | ASP172 |
| D | HIS189 |
| D | HOH2075 |
| site_id | AC5 |
| Number of Residues | 13 |
| Details | BINDING SITE FOR RESIDUE CPS D 1326 |
| Chain | Residue |
| B | ILE203 |
| B | THR238 |
| B | PHE245 |
| C | TYR151 |
| C | ALA211 |
| C | HOH2074 |
| D | ILE203 |
| D | ASN204 |
| D | LYS206 |
| D | THR238 |
| D | CPS1327 |
| D | HOH2148 |
| D | HOH2149 |
| site_id | AC6 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE CPS D 1327 |
| Chain | Residue |
| C | THR273 |
| D | TYR241 |
| D | LYS246 |
| D | GLU248 |
| D | CPS1326 |
| D | HOH2114 |
| site_id | AC7 |
| Number of Residues | 11 |
| Details | BINDING SITE FOR RESIDUE CPS C 1315 |
| Chain | Residue |
| A | ASN236 |
| A | THR238 |
| C | LYS71 |
| C | ILE203 |
| C | ASN204 |
| C | ASN205 |
| C | LEU237 |
| C | THR238 |
| C | TYR241 |
| C | HOH2111 |
| C | HOH2112 |
| site_id | AC8 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE SO4 D 1328 |
| Chain | Residue |
| D | PHE110 |
| D | VAL111 |
| D | THR112 |
| D | ASP163 |
| D | ALA164 |
| site_id | AC9 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE SO4 A 1317 |
| Chain | Residue |
| A | PRO179 |
| A | GLN180 |
| A | GLN181 |
| A | HOH2145 |
| C | ASP234 |
| C | SER235 |
| site_id | BC1 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE SO4 C 1316 |
| Chain | Residue |
| C | THR112 |
| C | ASP163 |
| C | ALA164 |
| C | HOH2008 |
| C | VAL111 |
| site_id | BC2 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE SO4 C 1317 |
| Chain | Residue |
| C | SER256 |
| C | ALA257 |
| C | SER258 |
| C | HOH2092 |
| C | HOH2113 |
| D | LYS260 |
| site_id | BC3 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE SO4 D 1329 |
| Chain | Residue |
| B | SER235 |
| D | ASP154 |
| D | GLN180 |
| D | ARG183 |
| D | HOH2069 |
| D | HOH2079 |
| site_id | BC4 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE SO4 D 1330 |
| Chain | Residue |
| D | LYS127 |
| D | GLU128 |
| D | HOH2053 |
| site_id | BC5 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE SO4 A 1318 |
| Chain | Residue |
| A | VAL111 |
| A | THR112 |
| A | ASP163 |
| A | ALA164 |
| B | LYS288 |
| site_id | BC6 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE SO4 D 1331 |
| Chain | Residue |
| D | ARG25 |
| D | LYS44 |
| D | ARG322 |
| D | HOH2005 |
| site_id | BC7 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE SO4 A 1319 |
| Chain | Residue |
| A | LYS64 |
| A | ASP311 |
| A | HOH2025 |
| A | HOH2026 |
| site_id | BC8 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE SO4 D 1332 |
| Chain | Residue |
| D | PHE249 |
| D | CYS251 |
| D | LYS260 |
| D | LYS264 |
| site_id | BC9 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE SO4 C 1318 |
| Chain | Residue |
| C | LYS261 |
| C | ARG265 |
| C | ASN283 |
| C | GLU284 |
| C | HOH2106 |
| site_id | CC1 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE SO4 A 1320 |
| Chain | Residue |
| A | SER235 |
| A | ASN236 |
| A | HOH2147 |
| C | ARG183 |
| site_id | CC2 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE SO4 C 1319 |
| Chain | Residue |
| C | LYS64 |
| C | ASP311 |
| site_id | CC3 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE SO4 A 1321 |
| Chain | Residue |
| A | SER256 |
| A | ALA257 |
| A | SER258 |
| A | HOH2128 |
| A | HOH2149 |
| D | LYS69 |
| site_id | CC4 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE SO4 B 1315 |
| Chain | Residue |
| B | LYS64 |
| B | ASP310 |
| site_id | CC5 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE SO4 B 1316 |
| Chain | Residue |
| B | ASN70 |
| B | PRO179 |
| B | GLN180 |
| site_id | CC6 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE SO4 B 1317 |
| Chain | Residue |
| A | LYS87 |
| A | ARG93 |
| B | SER256 |
| B | ALA257 |
| B | SER258 |
| B | HOH2155 |
| site_id | CC7 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE SO4 D 1333 |
| Chain | Residue |
| D | LYS64 |
| D | ASP310 |
| D | ASP311 |
| D | HOH2035 |
| site_id | CC8 |
| Number of Residues | 8 |
| Details | BINDING SITE FOR RESIDUE SO4 D 1334 |
| Chain | Residue |
| A | GLY20 |
| A | ILE21 |
| B | ARG49 |
| D | ARG25 |
| D | GLY34 |
| D | ARG35 |
| D | THR36 |
| D | LYS46 |
| site_id | CC9 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE SO4 A 1322 |
| Chain | Residue |
| A | ARG125 |
| A | TRP254 |
| site_id | DC1 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE SO4 C 1320 |
| Chain | Residue |
| C | LYS260 |
| C | LYS264 |
| C | HOH2087 |
Functional Information from PROSITE/UniProt
| site_id | PS00107 |
| Number of Residues | 24 |
| Details | PROTEIN_KINASE_ATP Protein kinases ATP-binding region signature. LGEGTFAKVKfArnvengdn..........VAIK |
| Chain | Residue | Details |
| A | LEU37-LYS60 |
| site_id | PS00108 |
| Number of Residues | 13 |
| Details | PROTEIN_KINASE_ST Serine/Threonine protein kinases active-site signature. VyHrDLKpeNLLL |
| Chain | Residue | Details |
| A | VAL150-LEU162 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 4 |
| Details | ACT_SITE: Proton acceptor => ECO:0000255|PROSITE-ProRule:PRU00159, ECO:0000255|PROSITE-ProRule:PRU10027 |
| Chain | Residue | Details |
| A | ASP154 | |
| B | ASP154 | |
| C | ASP154 | |
| D | ASP154 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 8 |
| Details | BINDING: BINDING => ECO:0000255|PROSITE-ProRule:PRU00159 |
| Chain | Residue | Details |
| A | LEU37 | |
| A | LYS60 | |
| B | LEU37 | |
| B | LYS60 | |
| C | LEU37 | |
| C | LYS60 | |
| D | LEU37 | |
| D | LYS60 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 4 |
| Details | MOD_RES: Phosphoserine => ECO:0000250|UniProtKB:Q93V58 |
| Chain | Residue | Details |
| A | SER176 | |
| B | SER176 | |
| C | SER176 | |
| D | SER176 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 4 |
| Details | MOD_RES: Phosphothreonine => ECO:0000250|UniProtKB:Q38997 |
| Chain | Residue | Details |
| A | THR190 | |
| B | THR190 | |
| C | THR190 | |
| D | THR190 |
