4BHT
Structural Determinants of Cofactor Specificity and Domain Flexibility in Bacterial Glutamate Dehydrogenases
Replaces: 2YFGFunctional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0004353 | molecular_function | glutamate dehydrogenase [NAD(P)+] activity |
| A | 0004354 | molecular_function | glutamate dehydrogenase (NADP+) activity |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0005829 | cellular_component | cytosol |
| A | 0006520 | biological_process | amino acid metabolic process |
| A | 0006536 | biological_process | glutamate metabolic process |
| A | 0006537 | biological_process | glutamate biosynthetic process |
| A | 0016491 | molecular_function | oxidoreductase activity |
| A | 0016639 | molecular_function | oxidoreductase activity, acting on the CH-NH2 group of donors, NAD or NADP as acceptor |
| A | 0042802 | molecular_function | identical protein binding |
| A | 0097216 | molecular_function | guanosine tetraphosphate binding |
| A | 1990148 | cellular_component | glutamate dehydrogenase complex |
| B | 0004353 | molecular_function | glutamate dehydrogenase [NAD(P)+] activity |
| B | 0004354 | molecular_function | glutamate dehydrogenase (NADP+) activity |
| B | 0005737 | cellular_component | cytoplasm |
| B | 0005829 | cellular_component | cytosol |
| B | 0006520 | biological_process | amino acid metabolic process |
| B | 0006536 | biological_process | glutamate metabolic process |
| B | 0006537 | biological_process | glutamate biosynthetic process |
| B | 0016491 | molecular_function | oxidoreductase activity |
| B | 0016639 | molecular_function | oxidoreductase activity, acting on the CH-NH2 group of donors, NAD or NADP as acceptor |
| B | 0042802 | molecular_function | identical protein binding |
| B | 0097216 | molecular_function | guanosine tetraphosphate binding |
| B | 1990148 | cellular_component | glutamate dehydrogenase complex |
| C | 0004353 | molecular_function | glutamate dehydrogenase [NAD(P)+] activity |
| C | 0004354 | molecular_function | glutamate dehydrogenase (NADP+) activity |
| C | 0005737 | cellular_component | cytoplasm |
| C | 0005829 | cellular_component | cytosol |
| C | 0006520 | biological_process | amino acid metabolic process |
| C | 0006536 | biological_process | glutamate metabolic process |
| C | 0006537 | biological_process | glutamate biosynthetic process |
| C | 0016491 | molecular_function | oxidoreductase activity |
| C | 0016639 | molecular_function | oxidoreductase activity, acting on the CH-NH2 group of donors, NAD or NADP as acceptor |
| C | 0042802 | molecular_function | identical protein binding |
| C | 0097216 | molecular_function | guanosine tetraphosphate binding |
| C | 1990148 | cellular_component | glutamate dehydrogenase complex |
| D | 0004353 | molecular_function | glutamate dehydrogenase [NAD(P)+] activity |
| D | 0004354 | molecular_function | glutamate dehydrogenase (NADP+) activity |
| D | 0005737 | cellular_component | cytoplasm |
| D | 0005829 | cellular_component | cytosol |
| D | 0006520 | biological_process | amino acid metabolic process |
| D | 0006536 | biological_process | glutamate metabolic process |
| D | 0006537 | biological_process | glutamate biosynthetic process |
| D | 0016491 | molecular_function | oxidoreductase activity |
| D | 0016639 | molecular_function | oxidoreductase activity, acting on the CH-NH2 group of donors, NAD or NADP as acceptor |
| D | 0042802 | molecular_function | identical protein binding |
| D | 0097216 | molecular_function | guanosine tetraphosphate binding |
| D | 1990148 | cellular_component | glutamate dehydrogenase complex |
| E | 0004353 | molecular_function | glutamate dehydrogenase [NAD(P)+] activity |
| E | 0004354 | molecular_function | glutamate dehydrogenase (NADP+) activity |
| E | 0005737 | cellular_component | cytoplasm |
| E | 0005829 | cellular_component | cytosol |
| E | 0006520 | biological_process | amino acid metabolic process |
| E | 0006536 | biological_process | glutamate metabolic process |
| E | 0006537 | biological_process | glutamate biosynthetic process |
| E | 0016491 | molecular_function | oxidoreductase activity |
| E | 0016639 | molecular_function | oxidoreductase activity, acting on the CH-NH2 group of donors, NAD or NADP as acceptor |
| E | 0042802 | molecular_function | identical protein binding |
| E | 0097216 | molecular_function | guanosine tetraphosphate binding |
| E | 1990148 | cellular_component | glutamate dehydrogenase complex |
| F | 0004353 | molecular_function | glutamate dehydrogenase [NAD(P)+] activity |
| F | 0004354 | molecular_function | glutamate dehydrogenase (NADP+) activity |
| F | 0005737 | cellular_component | cytoplasm |
| F | 0005829 | cellular_component | cytosol |
| F | 0006520 | biological_process | amino acid metabolic process |
| F | 0006536 | biological_process | glutamate metabolic process |
| F | 0006537 | biological_process | glutamate biosynthetic process |
| F | 0016491 | molecular_function | oxidoreductase activity |
| F | 0016639 | molecular_function | oxidoreductase activity, acting on the CH-NH2 group of donors, NAD or NADP as acceptor |
| F | 0042802 | molecular_function | identical protein binding |
| F | 0097216 | molecular_function | guanosine tetraphosphate binding |
| F | 1990148 | cellular_component | glutamate dehydrogenase complex |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE GOL A 1448 |
| Chain | Residue |
| A | GLU8 |
| A | LEU11 |
| A | ASN12 |
| A | GLN15 |
| A | ARG29 |
| site_id | AC2 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE GOL A 1449 |
| Chain | Residue |
| A | HOH2141 |
| A | HOH2156 |
| D | PRO123 |
| A | GLN386 |
| A | ASN387 |
| A | GLY392 |
| A | HOH2105 |
| site_id | AC3 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE GOL A 1450 |
| Chain | Residue |
| A | ARG156 |
| A | GOL1452 |
| A | HOH2078 |
| A | HOH2157 |
| B | SER187 |
| B | ASN188 |
| site_id | AC4 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE GOL A 1451 |
| Chain | Residue |
| A | ARG156 |
| A | GOL1452 |
| A | GOL1454 |
| A | HOH2079 |
| A | HOH2157 |
| A | HOH2158 |
| C | ARG156 |
| site_id | AC5 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE GOL C 1448 |
| Chain | Residue |
| C | GLN386 |
| C | ASN387 |
| C | LEU391 |
| C | GLY392 |
| C | HOH2141 |
| C | HOH2159 |
| F | PRO123 |
| site_id | AC6 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE GOL C 1449 |
| Chain | Residue |
| A | MET48 |
| C | VAL68 |
| C | ASN72 |
| C | LYS298 |
| C | EPE1451 |
| E | GLU140 |
| E | GLY141 |
| site_id | AC7 |
| Number of Residues | 9 |
| Details | BINDING SITE FOR RESIDUE GOL A 1452 |
| Chain | Residue |
| A | GOL1450 |
| A | GOL1451 |
| A | HOH2157 |
| A | HOH2159 |
| B | SER187 |
| B | ASN188 |
| B | ASN189 |
| C | LEU158 |
| C | THR162 |
| site_id | AC8 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE GOL C 1450 |
| Chain | Residue |
| C | GLN15 |
| C | ARG29 |
| C | HOH2012 |
| C | HOH2013 |
| site_id | AC9 |
| Number of Residues | 9 |
| Details | BINDING SITE FOR RESIDUE EPE C 1451 |
| Chain | Residue |
| C | VAL68 |
| C | ASP69 |
| C | ASP70 |
| C | ASN72 |
| C | ARG145 |
| C | GOL1449 |
| C | HOH2160 |
| E | ASP70 |
| E | ARG145 |
| site_id | BC1 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE GOL D 1448 |
| Chain | Residue |
| D | LEU11 |
| D | ASN12 |
| D | GLN15 |
| D | ARG29 |
| D | HOH2001 |
| D | HOH2011 |
| D | HOH2012 |
| site_id | BC2 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE GOL D 1449 |
| Chain | Residue |
| A | HOH2092 |
| B | HOH2080 |
| D | ARG156 |
| D | HOH2049 |
| site_id | BC3 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE GOL D 1450 |
| Chain | Residue |
| D | GLN386 |
| D | ASN387 |
| D | GLY392 |
| D | HOH2103 |
| E | THR121 |
| E | PRO123 |
| site_id | BC4 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE GOL E 1448 |
| Chain | Residue |
| C | HOH2078 |
| E | ARG156 |
| E | GOL1449 |
| E | HOH2053 |
| E | HOH2092 |
| F | GOL1448 |
| site_id | BC5 |
| Number of Residues | 8 |
| Details | BINDING SITE FOR RESIDUE GOL E 1449 |
| Chain | Residue |
| D | SER187 |
| D | ASN188 |
| D | HOH2058 |
| E | ARG156 |
| E | LEU158 |
| E | THR162 |
| E | GOL1448 |
| F | GOL1448 |
| site_id | BC6 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE GOL A 1453 |
| Chain | Residue |
| A | ARG156 |
| A | LEU158 |
| A | THR162 |
| A | GOL1454 |
| E | SER187 |
| E | ASN188 |
| E | ASN189 |
| site_id | BC7 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE GOL E 1450 |
| Chain | Residue |
| C | LEU302 |
| C | VAL303 |
| C | HOH2031 |
| E | ASP70 |
| E | SER139 |
| E | HOH2093 |
| site_id | BC8 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE GOL A 1454 |
| Chain | Residue |
| A | GOL1451 |
| A | GOL1453 |
| C | ARG156 |
| E | SER187 |
| E | ASN188 |
| site_id | BC9 |
| Number of Residues | 14 |
| Details | BINDING SITE FOR RESIDUE PG4 E 1451 |
| Chain | Residue |
| A | ILE88 |
| A | GLY89 |
| A | PRO123 |
| A | HOH2055 |
| E | LYS184 |
| E | THR190 |
| E | PHE201 |
| E | GLY202 |
| E | ASN387 |
| E | GLY392 |
| E | HOH2059 |
| E | HOH2060 |
| E | HOH2066 |
| E | HOH2084 |
| site_id | CC1 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE GOL F 1448 |
| Chain | Residue |
| D | SER187 |
| D | ASN188 |
| D | HOH2047 |
| E | GOL1448 |
| E | GOL1449 |
| F | ARG156 |
| F | HOH2054 |
| site_id | CC2 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE GOL F 1449 |
| Chain | Residue |
| F | GLU40 |
| F | ARG46 |
Functional Information from PROSITE/UniProt
| site_id | PS00074 |
| Number of Residues | 14 |
| Details | GLFV_DEHYDROGENASE Glu / Leu / Phe / Val dehydrogenases active site. LpmGGGKgGsdfDP |
| Chain | Residue | Details |
| A | LEU122-PRO135 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 6 |
| Details | ACT_SITE: Proton donor => ECO:0000255|PROSITE-ProRule:PRU10011 |
| Chain | Residue | Details |
| A | LYS128 | |
| B | LYS128 | |
| C | LYS128 | |
| D | LYS128 | |
| E | LYS128 | |
| F | LYS128 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 42 |
| Details | BINDING: BINDING => ECO:0000250 |
| Chain | Residue | Details |
| A | LYS92 | |
| B | LYS116 | |
| B | GLY167 | |
| B | THR211 | |
| B | ASN242 | |
| B | SER380 | |
| C | LYS92 | |
| C | GLN113 | |
| C | LYS116 | |
| C | GLY167 | |
| C | THR211 | |
| A | GLN113 | |
| C | ASN242 | |
| C | SER380 | |
| D | LYS92 | |
| D | GLN113 | |
| D | LYS116 | |
| D | GLY167 | |
| D | THR211 | |
| D | ASN242 | |
| D | SER380 | |
| E | LYS92 | |
| A | LYS116 | |
| E | GLN113 | |
| E | LYS116 | |
| E | GLY167 | |
| E | THR211 | |
| E | ASN242 | |
| E | SER380 | |
| F | LYS92 | |
| F | GLN113 | |
| F | LYS116 | |
| F | GLY167 | |
| A | GLY167 | |
| F | THR211 | |
| F | ASN242 | |
| F | SER380 | |
| A | THR211 | |
| A | ASN242 | |
| A | SER380 | |
| B | LYS92 | |
| B | GLN113 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 6 |
| Details | SITE: Important for catalysis |
| Chain | Residue | Details |
| A | ASP168 | |
| B | ASP168 | |
| C | ASP168 | |
| D | ASP168 | |
| E | ASP168 | |
| F | ASP168 |
