Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

4AUN

Crystal structure, recombinant expression and mutagenesis studies of the bifunctional catalase-phenol oxidase from Scytalidium thermophilum

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004096	molecular_function	catalase activity
A	0004601	molecular_function	peroxidase activity
A	0005829	cellular_component	cytosol
A	0006979	biological_process	response to oxidative stress
A	0020037	molecular_function	heme binding
A	0042744	biological_process	hydrogen peroxide catabolic process
A	0046872	molecular_function	metal ion binding
A	0098869	biological_process	cellular oxidant detoxification
B	0004096	molecular_function	catalase activity
B	0004601	molecular_function	peroxidase activity
B	0005829	cellular_component	cytosol
B	0006979	biological_process	response to oxidative stress
B	0020037	molecular_function	heme binding
B	0042744	biological_process	hydrogen peroxide catabolic process
B	0046872	molecular_function	metal ion binding
B	0098869	biological_process	cellular oxidant detoxification
C	0004096	molecular_function	catalase activity
C	0004601	molecular_function	peroxidase activity
C	0005829	cellular_component	cytosol
C	0006979	biological_process	response to oxidative stress
C	0020037	molecular_function	heme binding
C	0042744	biological_process	hydrogen peroxide catabolic process
C	0046872	molecular_function	metal ion binding
C	0098869	biological_process	cellular oxidant detoxification
D	0004096	molecular_function	catalase activity
D	0004601	molecular_function	peroxidase activity
D	0005829	cellular_component	cytosol
D	0006979	biological_process	response to oxidative stress
D	0020037	molecular_function	heme binding
D	0042744	biological_process	hydrogen peroxide catabolic process
D	0046872	molecular_function	metal ion binding
D	0098869	biological_process	cellular oxidant detoxification
E	0004096	molecular_function	catalase activity
E	0004601	molecular_function	peroxidase activity
E	0005829	cellular_component	cytosol
E	0006979	biological_process	response to oxidative stress
E	0020037	molecular_function	heme binding
E	0042744	biological_process	hydrogen peroxide catabolic process
E	0046872	molecular_function	metal ion binding
E	0098869	biological_process	cellular oxidant detoxification
F	0004096	molecular_function	catalase activity
F	0004601	molecular_function	peroxidase activity
F	0005829	cellular_component	cytosol
F	0006979	biological_process	response to oxidative stress
F	0020037	molecular_function	heme binding
F	0042744	biological_process	hydrogen peroxide catabolic process
F	0046872	molecular_function	metal ion binding
F	0098869	biological_process	cellular oxidant detoxification
G	0004096	molecular_function	catalase activity
G	0004601	molecular_function	peroxidase activity
G	0005829	cellular_component	cytosol
G	0006979	biological_process	response to oxidative stress
G	0020037	molecular_function	heme binding
G	0042744	biological_process	hydrogen peroxide catabolic process
G	0046872	molecular_function	metal ion binding
G	0098869	biological_process	cellular oxidant detoxification
H	0004096	molecular_function	catalase activity
H	0004601	molecular_function	peroxidase activity
H	0005829	cellular_component	cytosol
H	0006979	biological_process	response to oxidative stress
H	0020037	molecular_function	heme binding
H	0042744	biological_process	hydrogen peroxide catabolic process
H	0046872	molecular_function	metal ion binding
H	0098869	biological_process	cellular oxidant detoxification

Functional Information from PDB Data

site_id	AC1
Number of Residues	23
Details	BINDING SITE FOR RESIDUE HDD A 900

Chain	Residue
A	ARG79
A	PHE168
A	VAL228
A	HIS229
A	PHE345
A	LEU361
A	ARG365
A	SER368
A	TYR369
A	THR372
A	GLN373
A	VAL81
A	ARG376
A	HOH2085
A	HOH2122
A	HOH2308
A	HIS82
A	ARG119
A	GLY138
A	VAL153
A	GLY154
A	ASN155
A	PHE160

site_id	AC2
Number of Residues	23
Details	BINDING SITE FOR RESIDUE HDD B 900

Chain	Residue
B	ARG79
B	VAL81
B	HIS82
B	ARG119
B	GLY138
B	VAL153
B	GLY154
B	ASN155
B	PHE160
B	PHE168
B	VAL228
B	HIS229
B	PHE345
B	LEU361
B	ARG365
B	SER368
B	TYR369
B	THR372
B	GLN373
B	ARG376
B	HOH2057
B	HOH2090
B	HOH2234

site_id	AC3
Number of Residues	23
Details	BINDING SITE FOR RESIDUE HDD C 900

Chain	Residue
C	ARG79
C	VAL81
C	HIS82
C	ARG119
C	GLY138
C	VAL153
C	GLY154
C	ASN155
C	PHE160
C	PHE168
C	VAL228
C	HIS229
C	PHE345
C	LEU361
C	ARG365
C	SER368
C	TYR369
C	THR372
C	GLN373
C	ARG376
C	HOH2116
C	HOH2281
C	HOH2469

site_id	AC4
Number of Residues	24
Details	BINDING SITE FOR RESIDUE HDD D 900

Chain	Residue
D	ARG79
D	ALA80
D	VAL81
D	HIS82
D	ARG119
D	GLY138
D	VAL153
D	GLY154
D	ASN155
D	PHE160
D	PHE168
D	VAL228
D	HIS229
D	PHE345
D	LEU361
D	ARG365
D	SER368
D	TYR369
D	THR372
D	GLN373
D	ARG376
D	HOH2079
D	HOH2118
D	HOH2293

site_id	AC5
Number of Residues	23
Details	BINDING SITE FOR RESIDUE HDD E 900

Chain	Residue
E	GLY154
E	ASN155
E	PHE160
E	PHE168
E	VAL228
E	HIS229
E	PHE345
E	LEU361
E	ARG365
E	SER368
E	TYR369
E	THR372
E	GLN373
E	ARG376
E	HOH2038
E	HOH2064
E	HOH2205
E	ARG79
E	VAL81
E	HIS82
E	ARG119
E	GLY138
E	VAL153

site_id	AC6
Number of Residues	23
Details	BINDING SITE FOR RESIDUE HDD F 900

Chain	Residue
F	ARG79
F	VAL81
F	HIS82
F	ARG119
F	GLY138
F	VAL153
F	GLY154
F	ASN155
F	PHE160
F	PHE168
F	VAL228
F	HIS229
F	PHE345
F	LEU361
F	ARG365
F	SER368
F	TYR369
F	THR372
F	GLN373
F	ARG376
F	HOH2086
F	HOH2236
F	HOH2380

site_id	AC7
Number of Residues	23
Details	BINDING SITE FOR RESIDUE HDD G 900

Chain	Residue
G	ARG79
G	VAL81
G	HIS82
G	ARG119
G	GLY138
G	VAL153
G	GLY154
G	ASN155
G	PHE160
G	PHE168
G	VAL228
G	HIS229
G	PHE345
G	LEU361
G	ARG365
G	SER368
G	TYR369
G	THR372
G	GLN373
G	ARG376
G	HOH2057
G	HOH2099
G	HOH2269

site_id	AC8
Number of Residues	24
Details	BINDING SITE FOR RESIDUE HDD H 900

Chain	Residue
H	ARG79
H	VAL81
H	HIS82
H	ARG119
H	GLY138
H	VAL153
H	GLY154
H	ASN155
H	PHE160
H	PHE168
H	VAL228
H	HIS229
H	PHE345
H	LEU361
H	ARG365
H	SER368
H	TYR369
H	THR372
H	GLN373
H	ARG376
H	HOH2030
H	HOH2062
H	HOH2206
H	HOH2349

site_id	AC9
Number of Residues	6
Details	BINDING SITE FOR RESIDUE CA A 1700

Chain	Residue
A	HOH2334
A	HOH2335
A	HOH2519
A	HOH2520
B	HOH2254
B	HOH2256

site_id	BC1
Number of Residues	3
Details	BINDING SITE FOR RESIDUE CA A 1701

Chain	Residue
A	HIS190
A	SER192
A	HOH2521

site_id	BC2
Number of Residues	4
Details	BINDING SITE FOR RESIDUE CA B 1700

Chain	Residue
B	HIS190
B	ASP191
B	SER192
B	HOH2420

site_id	BC3
Number of Residues	4
Details	BINDING SITE FOR RESIDUE CA C 1700

Chain	Residue
C	HIS190
C	ASP191
C	SER192
C	HOH2470

site_id	BC4
Number of Residues	3
Details	BINDING SITE FOR RESIDUE CA D 1700

Chain	Residue
D	HIS190
D	SER192
D	HOH2468

site_id	BC5
Number of Residues	5
Details	BINDING SITE FOR RESIDUE CA E 1700

Chain	Residue
E	ARG178
E	HIS190
E	ASP191
E	SER192
E	HOH2341

site_id	BC6
Number of Residues	5
Details	BINDING SITE FOR RESIDUE CA F 1700

Chain	Residue
F	ARG178
F	HIS190
F	SER192
F	HOH2140
F	HOH2381

site_id	BC7
Number of Residues	3
Details	BINDING SITE FOR RESIDUE CA G 1700

Chain	Residue
G	HIS190
G	SER192
G	HOH2454

site_id	BC8
Number of Residues	3
Details	BINDING SITE FOR RESIDUE CA H 1700

Chain	Residue
H	HIS190
H	SER192
H	HOH2350

Functional Information from PROSITE/UniProt

site_id	PS00437
Number of Residues	9
Details	CATALASE_1 Catalase proximal heme-ligand signature. RLFSYlDTQ

Chain	Residue	Details
A	ARG365-GLN373

site_id	PS00438
Number of Residues	17
Details	CATALASE_2 Catalase proximal active site signature. FdHervpERavHarGAG

Chain	Residue	Details
A	PHE71-GLY87

site_id	PS00674
Number of Residues	20
Details	AAA AAA-protein family signature. VgILaTTSessaLDqAAqlR

Chain	Residue	Details
A	VAL552-ARG571

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)