4AUN
Crystal structure, recombinant expression and mutagenesis studies of the bifunctional catalase-phenol oxidase from Scytalidium thermophilum
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0004096 | molecular_function | catalase activity |
| A | 0004601 | molecular_function | peroxidase activity |
| A | 0005829 | cellular_component | cytosol |
| A | 0006979 | biological_process | response to oxidative stress |
| A | 0016491 | molecular_function | oxidoreductase activity |
| A | 0020037 | molecular_function | heme binding |
| A | 0042744 | biological_process | hydrogen peroxide catabolic process |
| A | 0046872 | molecular_function | metal ion binding |
| A | 0098869 | biological_process | cellular oxidant detoxification |
| B | 0004096 | molecular_function | catalase activity |
| B | 0004601 | molecular_function | peroxidase activity |
| B | 0005829 | cellular_component | cytosol |
| B | 0006979 | biological_process | response to oxidative stress |
| B | 0016491 | molecular_function | oxidoreductase activity |
| B | 0020037 | molecular_function | heme binding |
| B | 0042744 | biological_process | hydrogen peroxide catabolic process |
| B | 0046872 | molecular_function | metal ion binding |
| B | 0098869 | biological_process | cellular oxidant detoxification |
| C | 0004096 | molecular_function | catalase activity |
| C | 0004601 | molecular_function | peroxidase activity |
| C | 0005829 | cellular_component | cytosol |
| C | 0006979 | biological_process | response to oxidative stress |
| C | 0016491 | molecular_function | oxidoreductase activity |
| C | 0020037 | molecular_function | heme binding |
| C | 0042744 | biological_process | hydrogen peroxide catabolic process |
| C | 0046872 | molecular_function | metal ion binding |
| C | 0098869 | biological_process | cellular oxidant detoxification |
| D | 0004096 | molecular_function | catalase activity |
| D | 0004601 | molecular_function | peroxidase activity |
| D | 0005829 | cellular_component | cytosol |
| D | 0006979 | biological_process | response to oxidative stress |
| D | 0016491 | molecular_function | oxidoreductase activity |
| D | 0020037 | molecular_function | heme binding |
| D | 0042744 | biological_process | hydrogen peroxide catabolic process |
| D | 0046872 | molecular_function | metal ion binding |
| D | 0098869 | biological_process | cellular oxidant detoxification |
| E | 0004096 | molecular_function | catalase activity |
| E | 0004601 | molecular_function | peroxidase activity |
| E | 0005829 | cellular_component | cytosol |
| E | 0006979 | biological_process | response to oxidative stress |
| E | 0016491 | molecular_function | oxidoreductase activity |
| E | 0020037 | molecular_function | heme binding |
| E | 0042744 | biological_process | hydrogen peroxide catabolic process |
| E | 0046872 | molecular_function | metal ion binding |
| E | 0098869 | biological_process | cellular oxidant detoxification |
| F | 0004096 | molecular_function | catalase activity |
| F | 0004601 | molecular_function | peroxidase activity |
| F | 0005829 | cellular_component | cytosol |
| F | 0006979 | biological_process | response to oxidative stress |
| F | 0016491 | molecular_function | oxidoreductase activity |
| F | 0020037 | molecular_function | heme binding |
| F | 0042744 | biological_process | hydrogen peroxide catabolic process |
| F | 0046872 | molecular_function | metal ion binding |
| F | 0098869 | biological_process | cellular oxidant detoxification |
| G | 0004096 | molecular_function | catalase activity |
| G | 0004601 | molecular_function | peroxidase activity |
| G | 0005829 | cellular_component | cytosol |
| G | 0006979 | biological_process | response to oxidative stress |
| G | 0016491 | molecular_function | oxidoreductase activity |
| G | 0020037 | molecular_function | heme binding |
| G | 0042744 | biological_process | hydrogen peroxide catabolic process |
| G | 0046872 | molecular_function | metal ion binding |
| G | 0098869 | biological_process | cellular oxidant detoxification |
| H | 0004096 | molecular_function | catalase activity |
| H | 0004601 | molecular_function | peroxidase activity |
| H | 0005829 | cellular_component | cytosol |
| H | 0006979 | biological_process | response to oxidative stress |
| H | 0016491 | molecular_function | oxidoreductase activity |
| H | 0020037 | molecular_function | heme binding |
| H | 0042744 | biological_process | hydrogen peroxide catabolic process |
| H | 0046872 | molecular_function | metal ion binding |
| H | 0098869 | biological_process | cellular oxidant detoxification |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 23 |
| Details | BINDING SITE FOR RESIDUE HDD A 900 |
| Chain | Residue |
| A | ARG79 |
| A | PHE168 |
| A | VAL228 |
| A | HIS229 |
| A | PHE345 |
| A | LEU361 |
| A | ARG365 |
| A | SER368 |
| A | TYR369 |
| A | THR372 |
| A | GLN373 |
| A | VAL81 |
| A | ARG376 |
| A | HOH2085 |
| A | HOH2122 |
| A | HOH2308 |
| A | HIS82 |
| A | ARG119 |
| A | GLY138 |
| A | VAL153 |
| A | GLY154 |
| A | ASN155 |
| A | PHE160 |
| site_id | AC2 |
| Number of Residues | 23 |
| Details | BINDING SITE FOR RESIDUE HDD B 900 |
| Chain | Residue |
| B | ARG79 |
| B | VAL81 |
| B | HIS82 |
| B | ARG119 |
| B | GLY138 |
| B | VAL153 |
| B | GLY154 |
| B | ASN155 |
| B | PHE160 |
| B | PHE168 |
| B | VAL228 |
| B | HIS229 |
| B | PHE345 |
| B | LEU361 |
| B | ARG365 |
| B | SER368 |
| B | TYR369 |
| B | THR372 |
| B | GLN373 |
| B | ARG376 |
| B | HOH2057 |
| B | HOH2090 |
| B | HOH2234 |
| site_id | AC3 |
| Number of Residues | 23 |
| Details | BINDING SITE FOR RESIDUE HDD C 900 |
| Chain | Residue |
| C | ARG79 |
| C | VAL81 |
| C | HIS82 |
| C | ARG119 |
| C | GLY138 |
| C | VAL153 |
| C | GLY154 |
| C | ASN155 |
| C | PHE160 |
| C | PHE168 |
| C | VAL228 |
| C | HIS229 |
| C | PHE345 |
| C | LEU361 |
| C | ARG365 |
| C | SER368 |
| C | TYR369 |
| C | THR372 |
| C | GLN373 |
| C | ARG376 |
| C | HOH2116 |
| C | HOH2281 |
| C | HOH2469 |
| site_id | AC4 |
| Number of Residues | 24 |
| Details | BINDING SITE FOR RESIDUE HDD D 900 |
| Chain | Residue |
| D | ARG79 |
| D | ALA80 |
| D | VAL81 |
| D | HIS82 |
| D | ARG119 |
| D | GLY138 |
| D | VAL153 |
| D | GLY154 |
| D | ASN155 |
| D | PHE160 |
| D | PHE168 |
| D | VAL228 |
| D | HIS229 |
| D | PHE345 |
| D | LEU361 |
| D | ARG365 |
| D | SER368 |
| D | TYR369 |
| D | THR372 |
| D | GLN373 |
| D | ARG376 |
| D | HOH2079 |
| D | HOH2118 |
| D | HOH2293 |
| site_id | AC5 |
| Number of Residues | 23 |
| Details | BINDING SITE FOR RESIDUE HDD E 900 |
| Chain | Residue |
| E | GLY154 |
| E | ASN155 |
| E | PHE160 |
| E | PHE168 |
| E | VAL228 |
| E | HIS229 |
| E | PHE345 |
| E | LEU361 |
| E | ARG365 |
| E | SER368 |
| E | TYR369 |
| E | THR372 |
| E | GLN373 |
| E | ARG376 |
| E | HOH2038 |
| E | HOH2064 |
| E | HOH2205 |
| E | ARG79 |
| E | VAL81 |
| E | HIS82 |
| E | ARG119 |
| E | GLY138 |
| E | VAL153 |
| site_id | AC6 |
| Number of Residues | 23 |
| Details | BINDING SITE FOR RESIDUE HDD F 900 |
| Chain | Residue |
| F | ARG79 |
| F | VAL81 |
| F | HIS82 |
| F | ARG119 |
| F | GLY138 |
| F | VAL153 |
| F | GLY154 |
| F | ASN155 |
| F | PHE160 |
| F | PHE168 |
| F | VAL228 |
| F | HIS229 |
| F | PHE345 |
| F | LEU361 |
| F | ARG365 |
| F | SER368 |
| F | TYR369 |
| F | THR372 |
| F | GLN373 |
| F | ARG376 |
| F | HOH2086 |
| F | HOH2236 |
| F | HOH2380 |
| site_id | AC7 |
| Number of Residues | 23 |
| Details | BINDING SITE FOR RESIDUE HDD G 900 |
| Chain | Residue |
| G | ARG79 |
| G | VAL81 |
| G | HIS82 |
| G | ARG119 |
| G | GLY138 |
| G | VAL153 |
| G | GLY154 |
| G | ASN155 |
| G | PHE160 |
| G | PHE168 |
| G | VAL228 |
| G | HIS229 |
| G | PHE345 |
| G | LEU361 |
| G | ARG365 |
| G | SER368 |
| G | TYR369 |
| G | THR372 |
| G | GLN373 |
| G | ARG376 |
| G | HOH2057 |
| G | HOH2099 |
| G | HOH2269 |
| site_id | AC8 |
| Number of Residues | 24 |
| Details | BINDING SITE FOR RESIDUE HDD H 900 |
| Chain | Residue |
| H | ARG79 |
| H | VAL81 |
| H | HIS82 |
| H | ARG119 |
| H | GLY138 |
| H | VAL153 |
| H | GLY154 |
| H | ASN155 |
| H | PHE160 |
| H | PHE168 |
| H | VAL228 |
| H | HIS229 |
| H | PHE345 |
| H | LEU361 |
| H | ARG365 |
| H | SER368 |
| H | TYR369 |
| H | THR372 |
| H | GLN373 |
| H | ARG376 |
| H | HOH2030 |
| H | HOH2062 |
| H | HOH2206 |
| H | HOH2349 |
| site_id | AC9 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE CA A 1700 |
| Chain | Residue |
| A | HOH2334 |
| A | HOH2335 |
| A | HOH2519 |
| A | HOH2520 |
| B | HOH2254 |
| B | HOH2256 |
| site_id | BC1 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE CA A 1701 |
| Chain | Residue |
| A | HIS190 |
| A | SER192 |
| A | HOH2521 |
| site_id | BC2 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE CA B 1700 |
| Chain | Residue |
| B | HIS190 |
| B | ASP191 |
| B | SER192 |
| B | HOH2420 |
| site_id | BC3 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE CA C 1700 |
| Chain | Residue |
| C | HIS190 |
| C | ASP191 |
| C | SER192 |
| C | HOH2470 |
| site_id | BC4 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE CA D 1700 |
| Chain | Residue |
| D | HIS190 |
| D | SER192 |
| D | HOH2468 |
| site_id | BC5 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE CA E 1700 |
| Chain | Residue |
| E | ARG178 |
| E | HIS190 |
| E | ASP191 |
| E | SER192 |
| E | HOH2341 |
| site_id | BC6 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE CA F 1700 |
| Chain | Residue |
| F | ARG178 |
| F | HIS190 |
| F | SER192 |
| F | HOH2140 |
| F | HOH2381 |
| site_id | BC7 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE CA G 1700 |
| Chain | Residue |
| G | HIS190 |
| G | SER192 |
| G | HOH2454 |
| site_id | BC8 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE CA H 1700 |
| Chain | Residue |
| H | HIS190 |
| H | SER192 |
| H | HOH2350 |
Functional Information from PROSITE/UniProt
| site_id | PS00437 |
| Number of Residues | 9 |
| Details | CATALASE_1 Catalase proximal heme-ligand signature. RLFSYlDTQ |
| Chain | Residue | Details |
| A | ARG365-GLN373 |
| site_id | PS00438 |
| Number of Residues | 17 |
| Details | CATALASE_2 Catalase proximal active site signature. FdHervpERavHarGAG |
| Chain | Residue | Details |
| A | PHE71-GLY87 |
| site_id | PS00674 |
| Number of Residues | 20 |
| Details | AAA AAA-protein family signature. VgILaTTSessaLDqAAqlR |
| Chain | Residue | Details |
| A | VAL552-ARG571 |
