4AUM

Crystal structure, recombinant expression and mutagenesis studies of the bifunctional catalase-phenol oxidase from Scytalidium thermophilum

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004096	molecular_function	catalase activity
A	0004601	molecular_function	peroxidase activity
A	0006979	biological_process	response to oxidative stress
A	0020037	molecular_function	heme binding
A	0042744	biological_process	hydrogen peroxide catabolic process
A	0046872	molecular_function	metal ion binding
A	0098869	biological_process	cellular oxidant detoxification
B	0004096	molecular_function	catalase activity
B	0004601	molecular_function	peroxidase activity
B	0006979	biological_process	response to oxidative stress
B	0020037	molecular_function	heme binding
B	0042744	biological_process	hydrogen peroxide catabolic process
B	0046872	molecular_function	metal ion binding
B	0098869	biological_process	cellular oxidant detoxification
C	0004096	molecular_function	catalase activity
C	0004601	molecular_function	peroxidase activity
C	0006979	biological_process	response to oxidative stress
C	0020037	molecular_function	heme binding
C	0042744	biological_process	hydrogen peroxide catabolic process
C	0046872	molecular_function	metal ion binding
C	0098869	biological_process	cellular oxidant detoxification
D	0004096	molecular_function	catalase activity
D	0004601	molecular_function	peroxidase activity
D	0006979	biological_process	response to oxidative stress
D	0020037	molecular_function	heme binding
D	0042744	biological_process	hydrogen peroxide catabolic process
D	0046872	molecular_function	metal ion binding
D	0098869	biological_process	cellular oxidant detoxification

Functional Information from PDB Data

site_id	AC1
Number of Residues	23
Details	BINDING SITE FOR RESIDUE HDD A 900

Chain	Residue
A	ARG79
A	PHE168
A	VAL228
A	HIS229
A	PHE345
A	LEU361
A	ARG365
A	SER368
A	TYR369
A	THR372
A	GLN373
A	VAL81
A	ARG376
A	HOH2119
A	HOH2182
A	HOH2444
A	HIS82
A	ARG119
A	GLY138
A	VAL153
A	GLY154
A	ASN155
A	PHE160

---

site_id	AC2
Number of Residues	23
Details	BINDING SITE FOR RESIDUE HDD B 900

Chain	Residue
B	ARG79
B	VAL81
B	HIS82
B	ARG119
B	GLY138
B	VAL153
B	GLY154
B	ASN155
B	PHE160
B	PHE168
B	VAL228
B	HIS229
B	PHE345
B	LEU361
B	ARG365
B	SER368
B	TYR369
B	THR372
B	GLN373
B	ARG376
B	HOH2112
B	HOH2163
B	HOH2392

---

site_id	AC3
Number of Residues	24
Details	BINDING SITE FOR RESIDUE HDD C 900

Chain	Residue
C	ARG79
C	VAL81
C	HIS82
C	ARG119
C	GLY138
C	VAL153
C	GLY154
C	ASN155
C	PHE160
C	ALA165
C	PHE168
C	VAL228
C	HIS229
C	PHE345
C	LEU361
C	ARG365
C	SER368
C	TYR369
C	THR372
C	GLN373
C	ARG376
C	HOH2042
C	HOH2084
C	HOH2292

---

site_id	AC4
Number of Residues	24
Details	BINDING SITE FOR RESIDUE HDD D 900

Chain	Residue
D	ARG79
D	VAL81
D	HIS82
D	ARG119
D	GLY138
D	VAL153
D	GLY154
D	ASN155
D	PHE160
D	ALA165
D	PHE168
D	VAL228
D	HIS229
D	PHE345
D	LEU361
D	ARG365
D	SER368
D	TYR369
D	THR372
D	GLN373
D	ARG376
D	HOH2039
D	HOH2085
D	HOH2272

---

site_id	AC5
Number of Residues	7
Details	BINDING SITE FOR RESIDUE 1PE A 1698

Chain	Residue
A	HOH2770
C	GLU492
A	LYS235
A	ASP236
A	ASP237
A	HOH2146
A	HOH2330

---

site_id	AC6
Number of Residues	6
Details	BINDING SITE FOR RESIDUE CA A 910

Chain	Residue
A	HOH2473
A	HOH2474
A	HOH2768
A	HOH2769
B	HOH2419
D	HOH2286

---

site_id	AC7
Number of Residues	2
Details	BINDING SITE FOR RESIDUE CA C 1698

Chain	Residue
C	HIS190
C	SER192

---

site_id	AC8
Number of Residues	3
Details	BINDING SITE FOR RESIDUE CA B 1699

Chain	Residue
B	HIS190
B	ASP191
B	SER192

---

site_id	AC9
Number of Residues	3
Details	BINDING SITE FOR RESIDUE CA D 1698

Chain	Residue
D	HIS190
D	SER192
D	HOH2473

---

site_id	BC1
Number of Residues	4
Details	BINDING SITE FOR RESIDUE CA A 1699

Chain	Residue
A	HIS190
A	ASP191
A	SER192
A	HOH2771

---

site_id	BC2
Number of Residues	2
Details	BINDING SITE FOR RESIDUE CA C 1699

Chain	Residue
C	SER255
C	HOH2189

---

site_id	BC3
Number of Residues	2
Details	BINDING SITE FOR RESIDUE CA D 1699

Chain	Residue
D	SER255
D	HOH2168

---

site_id	BC4
Number of Residues	3
Details	BINDING SITE FOR RESIDUE CA B 1700

Chain	Residue
B	SER255
B	PHE476
B	HOH2267

---

site_id	BC5
Number of Residues	4
Details	BINDING SITE FOR RESIDUE CA B 1701

Chain	Residue
A	ARG449
A	HOH2569
B	THR437
B	HOH2694

---

site_id	BC6
Number of Residues	3
Details	BINDING SITE FOR RESIDUE CA A 1700

Chain	Residue
A	THR437
A	HOH2772
B	ARG449

---

Functional Information from PROSITE/UniProt

site_id	PS00437
Number of Residues	9
Details	CATALASE_1 Catalase proximal heme-ligand signature. RLFSYlDTQ

Chain	Residue	Details
A	ARG365-GLN373

---

site_id	PS00438
Number of Residues	17
Details	CATALASE_2 Catalase proximal active site signature. FdHervpERavHarGAG

Chain	Residue	Details
A	PHE71-GLY87

---

site_id	PS00674
Number of Residues	20
Details	AAA AAA-protein family signature. VgILaTTSessaLDqAAqlR

Chain	Residue	Details
A	VAL552-ARG571

---